Differential Dopaminergic Modulation of the Antennal Lobe of Drosophila melanogaster

Differential Dopaminergic Modulation of the Antennal Lobe of Drosophila melanogaster | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Differential Dopaminergic Modulation of the Antennal Lobe of Drosophila melanogaster Moshe Parnas, Gavriel Avishai This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6737374/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 12 Nov, 2025 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Abstract Olfaction plays a key role in the ability of organisms to monitor their ever-changing chemical environment. Dopamine is a key modulator of the mammalian and insect nervous systems, including the fruit fly Drosophila melanogaster. While there are few indications for dopamine signaling in the fly’s first-order olfactory processing center, the antennal lobe (AL), the role of dopamine in Drosophila early olfactory processing remains unknown. Here we reveal broad dopamine receptor expression in key AL cell types. Dopamine application differentially modulates AL output in an odor-dependent manner. Furthermore, we identify AL-innervating neurons expressing the marker for dopaminergic cells, tyrosine hydroxylase, and show that activation of these neurons leads to dopamine release in the AL. Finally, we find that endogenous dopamine release affects AL output in a similar manner to dopamine application. Biological sciences/Neuroscience/Olfactory system Biological sciences/Neuroscience/Neural circuits Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SuppwithreferencesDONEreducedwithTableS1.pdf Supplementary material Cite Share Download PDF Status: Published Journal Publication published 12 Nov, 2025 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6737374","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":467176916,"identity":"c328cdcb-0fa9-4b28-bd3c-87d8dc13a403","order_by":0,"name":"Moshe Parnas","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA80lEQVRIiWNgGAWjYBADOTS+gQVBLcY8UKUwLRIEtST2oGphwK1Fd3bzs88FNXXp+9nPGD7mYfgjb3CA+eEHhgLcWszuHDOePePY4dwenhxjwxkMBoYbDrAZS+BzmNmNBGNmHrYDuT0MOWYSHxgMGDccYDDD6xezG+mfmXn+1aXz8L8x/5HAYGC/4QD7NwJacoyZeduYE3gkcswYgLYkbjjAQ8CWO2eKmXn7Dhv23HhWLDnDwDh55mGeYokEfFput29m5vlWJ8/en7zxM0+FnG3f8faNHz78scGpBS38QdHCDMQJuDXgi7JRMApGwSgYBVAAAMISSNVlrAudAAAAAElFTkSuQmCC","orcid":"","institution":"Tel Aviv University","correspondingAuthor":true,"prefix":"","firstName":"Moshe","middleName":"","lastName":"Parnas","suffix":""},{"id":467176917,"identity":"b3bbd25e-a63a-492b-8167-849e4dd59a70","order_by":1,"name":"Gavriel Avishai","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Gavriel","middleName":"","lastName":"Avishai","suffix":""}],"badges":[],"createdAt":"2025-05-24 07:12:52","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6737374/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6737374/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s42003-025-08922-y","type":"published","date":"2025-11-12T05:00:00+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":95809956,"identity":"8e5b6aa7-97a5-4c60-ac09-397322c8cebd","added_by":"auto","created_at":"2025-11-13 08:51:06","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":6236072,"visible":true,"origin":"","legend":"Article File","description":"","filename":"textfinalwithfiguresreduced.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6737374/v1_covered_70e90bba-3dc5-4a92-ac43-580e11d36b3b.pdf"},{"id":84250724,"identity":"81fd641b-dc02-40b7-a618-509489806e88","added_by":"auto","created_at":"2025-06-09 18:19:03","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":2914273,"visible":true,"origin":"","legend":"Supplementary material","description":"","filename":"SuppwithreferencesDONEreducedwithTableS1.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6737374/v1/eb30b67ca9a72da077aa6e1a.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"\u003cp\u003eDifferential Dopaminergic Modulation of the Antennal Lobe of \u003cem\u003eDrosophila melanogaster\u003c/em\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-6737374/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6737374/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Olfaction plays a key role in the ability of organisms to monitor their ever-changing chemical environment. 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