The effect of peritoneal macrophage incubates on the spermatozoa assay.

Karol J. Chacho; K J Chacho; Paul J. Andresen; P J Andresen; Antonio Scommegna; A Scommegna

doi:10.1016/s0015-0282(16)59490-4

The effect of peritoneal macrophage incubates on the spermatozoa assay.

Karol J. Chacho, K J Chacho, Paul J. Andresen, P J Andresen, Antonio Scommegna, A Scommegna

Fertility and Sterility · 1987 · vol. 48(4) , pp. 694–6 · doi:10.1016/s0015-0282(16)59490-4 · PMID:3653426 · W2411839173

article OA: closed CC0 ⤵ 7 in-corpus citations

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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

Medium incubated with peritoneal macrophages significantly decreased sperm penetration in a dose-dependent manner, with the active substance being heat-stable and released by both live and dead macrophages.

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Abstract

PFM have been implicated as a possible cause of infertility in endometriosis. Previous work from our laboratory has indicated that medium incubated with PFM caused a significant decrease in sperm penetration when added to the sperm penetration assay (SPA). To further delineate this finding, medium incubated with killed macrophages, heat-inactivated medium, and various concentrations of macrophage media were added to the SPA. Analysis of the data implied that there is a 1.536% decrease in penetration for every 1% increase in the concentration of PFM medium. Heat-inactivated medium and medium incubated with dead PFM both produced penetrations statistically different from the control. We conclude that medium incubated with PFM is capable of decreasing sperm penetration as measured in the SPA in a dose-dependent fashion. Furthermore, the substance responsible for this decrease appears to be heat-stable and released from dead as well as live macrophages.

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Condition tags

endometriosisinfertility

MeSH descriptors

Ascitic Fluid Endometriosis Infertility, Female Macrophages Sperm-Ovum Interactions Animals Ascitic Fluid Cells, Cultured Cricetinae Culture Media Endometriosis Endometriosis Endometriosis Female Hot Temperature Humans Infertility, Female Macrophages Male

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References (4)

Increased activation of pelvic macrophages in infertile women with mild endometriosis via openalex
Peritoneal fluid in patients with and without endometriosis: Prostanoids and macrophages and their effect on the spermatozoa penetration assay via openalex
W2409510670 via openalex
W2409896932 via openalex

Cited by (7)

Source provenance

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openalex: last seen: 2026-06-10T17:14:06.276822+00:00
pubmed: last seen: 2026-05-13T22:09:25.758913+00:00

License: CC0 · commercial use OK

[1] Increased activation of pelvic macrophages in infertile women with mild endometriosis via openalex

[2] Peritoneal fluid in patients with and without endometriosis: Prostanoids and macrophages and their effect on the spermatozoa penetration assay via openalex

[3] W2409510670 via openalex

[4] W2409896932 via openalex