Reconstructing the Holy Loch Ecosystem: The Holy Loch Food Web Project A foundational framework for a long-term ecosystem census integrating barcoding, environmental DNA, classical taxonomy, and niche architecture.

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Abstract

The Holy Loch Food Web Project has the single aim of clarifying all of the definable taxa and their associated ecological niches in our entire ecosystem comprising sea loch, saltmarsh, temperate rainforest, vegetated shingle, carr woodland and freshwater swamp. Although a simple idea, in reality, there are huge numbers of hurdles to get there. This note founds the whole project which will comprise, probably, hundreds of checklists from fungal, plant and animal taxa, updated as results from ongoing DNA barcoding via UK BIOSCAN led by the Wellcome Sanger Centre, eDNA metabarcoding and updated taxonomic understanding continue to clarify my simple species inventory. The project has no obvious end point. At the time of writing on 30th December 2025, the total number of eukaryote species listed at the Holy Loch exceeds 3000, and that is before we embark on eDNA techniques for soil fungi and large, unexplored taxa such as Nematoda. I expect the number of species to increase significantly in future.
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This is a Preprint and has not been peer reviewed. This is version 1 of this Preprint. You must log in to post a comment. There are no comments or no comments have been made public for this article. This is a Preprint and has not been peer reviewed. This is version 1 of this Preprint. Add a Comment You must log in to post a comment. Comments There are no comments or no comments have been made public for this article. The Holy Loch Food Web Project has the single aim of clarifying all of the definable taxa and their associated ecological niches in our entire ecosystem comprising sea loch, saltmarsh, temperate rainforest, vegetated shingle, carr woodland and freshwater swamp. Although a simple idea, in reality, there are huge numbers of hurdles to get there. This note founds the whole project which will comprise, probably, hundreds of checklists from fungal, plant and animal taxa, updated as results from ongoing DNA barcoding via UK BIOSCAN led by the Wellcome Sanger Centre, eDNA metabarcoding and updated taxonomic understanding continue to clarify my simple species inventory. The project has no obvious end point. At the time of writing on 30th December 2025, the total number of eukaryote species listed at the Holy Loch exceeds 3000, and that is before we embark on eDNA techniques for soil fungi and large, unexplored taxa such as Nematoda. I expect the number of species to increase significantly in future. https://doi.org/10.32942/X2G94K Life Sciences biodiversity census, eDNA, DNA barcoding, long-term monitoring, Holy Loch, temperate rainforest, sea loch, independent research, provisional taxonomy, Biodiversity Index Numbers, BIN clusters, Ecosystem, saltmarsh, niche architecture, curiosity-driven science Published: 2025-12-31 06:02 Last Updated: 2025-12-31 06:02 CC BY Attribution 4.0 International Language: English

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