Primary mouse hepatocyte isolation
preprint
OA: closed
Abstract
Abstract This protocol is intended to isolate primary hepatocytes from mus musculus (mouse) for the purposes of culturing primary hepatocytes. Hepatocytes can be used for approximately 5 days from the time of isolation, and cannot be frozen for future use. A single isolation from a single mouse, including time to prepare reagents, takes approximately 2 hours.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00