SPECTRE-Plex: an automated, fast, high-resolution-enabled approach for multiplexed cyclic imaging and tissue spatial analysis

Abstract Mapping the spatial organization of tissues is critical to understanding organ biology in health and disease. Developments in multiplexed antibody-based, fluorescence labelling methods have provided unique insights into tissue microenvironments. However, many current methods have a variety of limitations that reduce their practical utilization. To address this, we developed SPECTRE-Plex; an end-to-end solution based on a series of methods that significantly improve speed, automation, and resolution of cyclic multiplex immunofluorescence imaging. Competing Interest Statement The authors (JRT, MDA) are named inventors on a patent application (PCT/US24/31703) related to the methods and designs described in this manuscript. Footnotes Conflict of interest statement: The authors (JRT, MDA) are named inventors on a patent application (PCT/US24/31703) related to the methods and designs described in this manuscript.