Stable isotope tracing technology reveals the water allocation mechanism in Populus euphratica seedlings

Stable isotope tracing technology reveals the water allocation mechanism in Populus euphratica seedlings | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 5 September 2025 V1 Latest version Share on Stable isotope tracing technology reveals the water allocation mechanism in Populus euphratica seedlings Authors : Wusong Li , Yuchen Wang , Junhao Tian , Huifang Wang , Zhoukang Li , Yudong Chen , and Guanghui Lv 0000-0002-2382-776X [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175704137.77349333/v1 145 views 124 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Roots and leaves are crucial organs for plant water uptake, yet existing research on water utilization has largely focused on either root or foliar absorption in isolation. This study employed stable isotopes D and 18 O tracing techniques to elucidate the temporal utilization patterns and allocation ratios of water absorbed by both roots and leaves within Populus euphratica seedlings. Results indicated that one hour after humidification treatment, δ D abundance values in leaves of both C1 and C2 P. euphratica seedlings were significantly higher than initial values ( P < 0.001), thus confirming direct foliar water uptake (FWU), likely from fog or mist. Aboveground organ water utilization exhibited distinct temporal dynamics: peak water uptake in C1R1 shoots, leaves, and stems occurred between 72 and 132 hours post-humidification. Overall, C2R3 demonstrated higher utilization due to abundant soil moisture. Canopy humidification markedly altered water allocation strategies within belowground organs. Specifically, for C1R1-T1 conditions, water was preferentially allocated to belowground organs before transferring to aboveground parts; C1R2-T1 conditions showed uniform allocation; while C1R3 under T1 conditions prioritized supply to belowground organs. Furthermore, humidification intensity significantly influenced allocation patterns: C2 showed a markedly higher proportion of root water uptake (RWU) investment in leaves compared to C1, with C2R3-T2 contributing up to 11.3% of its total water absorption to leaves. Moreover, when FWU was sufficient, it could reciprocally supply soil moisture. These findings provide isotopic evidence for the water adaptation mechanisms of P. euphratica seedlings in arid regions. Supplementary Material File (manuscript.docx) Download 6.88 MB Information & Authors Information Version history V1 Version 1 05 September 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords canopy humidification foliar water uptake root water uptake water allocation ratio water relations xylem transport Authors Affiliations Wusong Li Xinjiang University College of Ecology and Environment View all articles by this author Yuchen Wang Xinjiang University College of Ecology and Environment View all articles by this author Junhao Tian Xinjiang University College of Ecology and Environment View all articles by this author Huifang Wang Xinjiang University College of Ecology and Environment View all articles by this author Zhoukang Li Xinjiang Agricultural University View all articles by this author Yudong Chen Xinjiang University College of Ecology and Environment View all articles by this author Guanghui Lv 0000-0002-2382-776X [email protected] Xinjiang University College of Ecology and Environment View all articles by this author Metrics & Citations Metrics Article Usage 145 views 124 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Wusong Li, Yuchen Wang, Junhao Tian, et al. Stable isotope tracing technology reveals the water allocation mechanism in Populus euphratica seedlings. Authorea . 05 September 2025. DOI: https://doi.org/10.22541/au.175704137.77349333/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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