Negative association of fat mass index and Alzheimer’s disease cerebrospinal fluid biomarkers in cognitively impaired patients

Negative association of fat mass index and Alzheimer’s disease cerebrospinal fluid biomarkers in cognitively impaired patients | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Negative association of fat mass index and Alzheimer’s disease cerebrospinal fluid biomarkers in cognitively impaired patients Karl Götze, Agathe Vrillon, Manuel Sanchez, Inès Petit-Damico, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7980996/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 22 Jan, 2026 Read the published version in Alzheimer's Research & Therapy → Version 1 posted 15 You are reading this latest preprint version Abstract INTRODUCTION Alzheimer's disease (AD) is associated with early weight loss, with unclear underlying mechanisms. Using real-world data, we aimed to investigate the links between biological AD profile, body composition, and nutritional status in patients with cognitive impairment. METHODS Cross-sectional study of patients with cognitive decline explored with cerebrospinal fluid (CSF) AD biomarkers and bioelectrical impedance analysis. Nutritional status was assessed using GLIM criteria. Analyses were stratified by sex and adjusted for age, MMSE, comorbidities, and the presence of a caregiver at home. RESULTS Among 420 patients, the fat mass index was positively associated with the Aβ42/40 ratio and negatively with pTau181 levels (p < 0.05). Malnutrition was more frequent in women than men (p = 0.0059). In women, malnutrition was associated with higher t-Tau and pTau181 levels (p < 0.02). In both sexes, pTau181 levels were inversely associated with muscle mass (p = 0.041). CONCLUSION These results suggest possible pathophysiological links between AD CSF biomarkers and nutritional status. Alzheimer’s disease Neurofilament proteins Cognitive domains performance Neuroimaging Neurodegenerative diseases Cognitive decline Figures Figure 1 Figure 2 1. Background A large body of evidence links nutritional status and body composition with the pathophysiology of neurodegenerative disorders, specifically in Alzheimer’s disease (AD). Chronic inflammation, altered energy metabolism, and dysregulation of appetite-related hormones were suggested to explain the association between weight loss and AD [1]. In addition, reciprocal relationships between nutritional status and cognitive decline were consistently reported. Notably, unintentional weight loss or lower Body Mass Index (BMI) were described several years before the onset of clinically detectable neurocognitive disorders, as evidenced by large-scale prospective cohort studies [2–5]. In AD, accelerated cognitive decline was also reported in individuals with notable weight loss during the first year of follow-up [6] or with lower BMI at baseline [7]. Similar results were found in a general population cohort of 3035 patients, in which higher baseline BMI and smaller weight loss were associated with a slower rate of cognitive decline [8]. These findings are further supported by the results of the Dominantly Inherited Alzheimer Network (DIAN) study[9], in which asymptomatic carriers began to lose weight approximately 11 years earlier than asymptomatic non-carriers. Moreover, in the same cohort, lower BMI in the preclinical stage was associated with earlier symptom onset and higher amyloid burden. The use of bioelectrical impedance analysis assesses the body composition by notably estimating lean mass, muscle mass, and fat mass. Indeed, low muscle mass is a phenotypic criterion for sarcopenia and for malnutrition[10] [11]. Findings about the association of incident dementia and body composition are inconsistent in the current literature. Two general population studies based on the UK Biobank cohort reported an inverse association between incident dementia and the fat-to-muscle ratio [12,13]. Other studies reported incident dementia to be associated with lower lean mass [14]. In contrast, other results found no association between incident dementia and body composition [15] or fat mass [14]. A few studies examined body composition specifically in AD. They reported lower lean mass in AD patients compared to controls [16], a higher prevalence of sarcopenia in moderate versus mild AD [17], and in AD patients compared with controls [18,19]. Interestingly, lower fat mass and lower lean mass were also associated with lower cortical thickness in AD-affected brain regions [20,21]. However, in those studies, AD diagnoses were made according to former AD diagnosis criteria (Mc Kahn 1984) without AD CSF biomarkers. Using CSF biomarker evidence [22,23], a publication by Doorduijn et al. observed a significant inverse association between phosphorylated Tau on threonine 181 (pTau181) and total-Tau (t-tau) with fat-free mass index, but no association with amyloid biomarkers [24] in 558 participants, including AD, mild cognitive impairment, and controls. They did not explore the sex differences. Overall, the available evidence on the relationship between cognitive decline, nutritional status, and biological biomarkers of AD remains limited, particularly regarding to sex differences and possible confounding factors. In the present study, we aimed to examine the relationships between AD cerebrospinal fluid (CSF) biomarkers, nutritional status, and body composition. Our objectives were: (1) to investigate the prevalence of malnutrition and low muscle mass in cognitive disorders; (2) to examine the association between AD CSF biomarkers and body composition, possibly suggesting a potential pathophysiological link, and (3) to identify the factors associated with malnutrition in these patients. 2. Methods 2.1. Study Design and Participants We performed a prospective multicentric study including patients evaluated for neurocognitive disorders who underwent CSF analysis for AD biomarker testing. Patients were enrolled from January 2022 to July 2025 at three centers at the Université Paris Cité GHU AP-HP Nord, Paris, France, including Lariboisière Hospital, a tertiary memory clinic, and two geriatric day-care hospitals: Bretonneau and Fernand Widal. 2.1.1. Inclusion and Exclusion Criteria All patients who had a lumbar puncture for CSF AD biomarker measurement and signed a written informed consent for the BioCogBank study (see § 2.6) were included. Patients with conditions likely to cause inflammation, such as active cancer, infectious diseases, and chronic inflammatory diseases, and any chronic gastrointestinal condition, that adversely impact food assimilation or absorption, were excluded. 2.1.2. Cognitive disease diagnostic assessment In each center, patients were evaluated by multidisciplinary teams composed of geriatricians, neurologists, neuropsychologists, biochemists, and neuroradiologists. Diagnoses were established after multidisciplinary team assessment including examination of CSF biomarkers, MRI and fluorodeoxyglucose positron emission tomography (PET) imaging. AD was diagnosed using the most recent diagnostic criteria for AD [25]. Other neurodegenerative diseases (OND) included dementia with Lewy Bodies (DLB) [26], frontotemporal dementia (FTD) [27], supranuclear palsy [28], corticobasal degeneration [29] or patients with cognitive decline and brain atrophy or hypometabolism on PET considered to have a neurodegenerative process after multidisciplinary case review. Patients were classified as having a non-neurodegenerative disorder (NND) if they did not meet criteria for neurodegenerative diseases (as defined above) and were considered as non-neurodegenerative by the clinician in charge, after multidisciplinary team assessment, including evaluation of CSF biomarkers, MRI, PET imaging, and cognitive follow-up. Patients without a confirmed diagnosis were classified as “unclassified dementia” and were excluded from the diagnostic-subgroup analysis. 2.2. CSF Biomarkers analysis After lumbar puncture, CSF samples were centrifuged at 1000 g for 10 min at 4°C within 2 h of collection and then aliquoted into 0.5 mL polypropylene tubes before being stored at − 80°C for further analysis. CSF t-tau, pTau181, and Aβ42 measurements were conducted in the Biochemistry Unit of Lariboisière Hospital using Elecsys® assays on the cobas e601 analyser (Roche Diagnostics). CSF Aβ40 measurement was performed using an Innotest ELISA assay (Fujirebio, Ghent, Belgium). Patients were considered as amyloid positive (A+) according to Aβ 42/40 ratio below 0.083, tau positive (T+) if pTau181 level was over 22 pg/mL and neurodegeneration positive (N+) if t-tau was over 220 pg/mL. Lumbar puncture for AD biomarker measurements was performed within six months of the body composition assessment (mean delay: 0.55 ± 1.94 months) 2.3. Nutritional status determination and bioelectrical impedance measurements Anthropometric measurements (weight and height) were obtained using standardized equipment. Multifrequency bioelectrical impedance analysis (BIA) was performed within six months before or after the lumbar puncture, using the InBody S10®. The data collected on fat mass, fat-free mass, and appendicular skeletal muscle were used to determine: Fat mass index: Fat mass (kg) / height (m²) Fat-free mass index: Fat-free mass (kg) / height(m²) Appendicular skeletal muscle mass (ASM, kg) Appendicular skeletal muscle mass index: appendicular skeletal muscle (kg)/ height (m²) Nutritional status, classified as no malnutrition, moderate malnutrition or severe malnutrition, was determined according to the 2019 Global Leadership Initiative on Malnutrition (GLIM) [10], requiring the combination of at least one phenotypic criteria and at least one aetiologic criteria. The phenotypic criteria are low BMI (< 20 for patients under 70 years old and 10% or low muscle mass. Low muscle mass was determined using the sex-specific thresholds of the revised European consensus on definition and diagnosis of sarcopenia, considering appendicular skeletal muscle mass < 20 kg in men or 15 kg in women and appendicular skeletal muscle mass index < 7 kg/m² in men or 5.5 kg/m² in women [30]. The aetiologic criteria for malnutrition were reduced appetite (using the Mini-Nutritional Assessment short form specific item), chronic diseases and major neurocognitive disorders. Based on blood tests, patients with biologically detectable inflammation or malabsorption were excluded. 2.4. Other Variables Additional variables, potentially affecting nutritional status and body composition, were also recorded: Functional status assessed by the 4-item Instrumental Activities of Daily Living (IADL) score [31] and the Activities of Daily Living (ADL) score [32] Home assistance evaluated with the presence of professional help at home (yes/no), and the presence of a relative living at home with the patient (yes/no) Comorbidities assessed using the Charlson Comorbidity Index (CCI) score [33]. The modified CCI was calculated excluding the age component to assess the comorbidity burden independently of age Cognitive impairment severity was measured using the Mini-Mental State Examination (MMSE) score [34]. 2.5. Statistical Analyses 2.5.1. Descriptive Analyses Due to substantial sex-related differences in body composition parameters, analyses were stratified by sex. Based on a hypothesis of a moderate correlation (r=-0.2) between CSF Aβ 42/40 ratio and fat mass[24] 193 patients are needed to reach a power of 80% with an alpha-risk at 5% (using Fisher Z transformation method) in each sex, meaning a total of 386 patients needed. Categorical variables were compared using the χ² test, or Fisher's exact test as appropriate. Continuous variables were compared using Student's t-test for variables following a parametric distribution and presented using mean ± standard deviation (SD). Variables of non-parametric distribution, of MMSE, ADL, and IADL scores were compared using the Wilcoxon test and presented using the median (1st ; 3rd quartiles). 2.5.2. Multivariate Analyses Missing data for covariates (age, modified CCI, presence of a caregiver at home) were treated by multiple imputation. All numerical variables were centered and scaled considering the mean and standard deviation of the population, both for linear and logistic regressions. Linear regression models examining BMI, fat mass index, fat-free mass index, and appendicular skeletal muscle mass index on the one hand, and CSF Aβ-42/Aβ-40 ratio, pTau181, and t-tau on the other hand, were adjusted for age, modified CCI score, MMSE score, and the presence of a caregiver at home, as clinically relevant variables, linked to malnutrition. Diagnostic subgroup analyses were performed to evaluate the relationship between AD CSF biomarkers and body composition metrics in each diagnosis group. These analyses were also adjusted for sex, age, modified CCI score, MMSE score, and presence of a caregiver at home, but not stratified by sex. The association between malnutrition and CSF AD biomarkers was assessed using multiple logistic regression, adjusting for age, modified CCI score, MMSE score, and presence of a caregiver at home. Analysis across the global population was also adjusted for sex. All multivariate analyses were stratified by sex to account for sex-specific differences in fat mass and lean mass. In all analyses, the type I error risk α was set at 0.05 and the power at 80%. Statistical analyses were performed using the R software (Version 4.3.1). 2.6. Ethics All data and samples analyzed were part of the BioCogBank study (NCT06244875). All participants gave written informed consent. This study was approved by the “Comité de Protection des Personnes Est III” (2023-A01413-42, 23-10-02) and by the "Commission Nationale Informatique et Libertés" (CNIL) 3. Results 3.1. Population’s characteristics Patients’ characteristics are presented in Table 1 . A total of 420 participants were included: 240 (57.1%) women and 180 (42.9%) men. Our study sample mainly comprised mild-stage neurocognitive disorders, with a median MMSE of 23 [interquartile range 19; 26]. The most frequent diagnosis was AD (57.4%), followed by NND (13.6%), OND (12.1%), and 16.9% of the population had unclassified dementia at the time of the study. Women had a lower comorbidity index (mean CCI score 4.09 ± 1.38 vs 4.51 ± 1.66 for men, p = 0.0065) and lived alone more often than men (52.1% vs 21.1%, p < 0.001). 3.2. Prevalence of malnutrition and muscle mass loss in neurocognitive disorders Regarding nutritional assessment, malnutrition was diagnosed in 31.7% of the patients, including 15.5% with severe malnutrition ( Table 1 ). Low muscle mass was observed in 18.1% of the patients, low BMI in 27.1% and > 10% weight loss in 11.7%. Women were more frequently malnourished than men (37.1% vs 24.4%, p = 0.0059). The prevalence of severe malnutrition was higher in women (20.8%) than in men (8.3%, p = 0.0034). In addition, women also had a more frequent lower muscle mass (22.9%) than men (11.7%), p = 0.0033. 3.3. Association between CSF AD biomarkers and body composition All data are presented in Table 2 and Fig. 1 . Table 2 Association of Aβ-42/Aβ-40 ratio, pTau 181, and total Tau with body composition according to sex. Women Men Aβ42/40 ratio pTau 181 Total Tau Aβ42/Aβ40 ratio pTau 181 Total Tau Estimate (CI) p Estimate (CI) p Estimate (CI) p Estimate (CI) p Estimate (CI) p Estimate (CI) p BMI Model 1 0.15 (0.03 ; 0.28) 0.018 -0.19 (-0.31 ; -0.06) 0.004 -0.19 (-0.32 ; -0.07) 0.003 0.24 (0.09 ; 0.38) 0.001 -0.11 (-0.26 ; 0.03) 0.12 -0.13 (-0.28 ; -0.01) 0.073 Model 2 0.12 (-0.01 ; 0.26) 0.071 -0.19 (-0.32 ; -0.07) 0.003 -0.21 (-0.33 ; -0.08) 0.002 0.23 (0.08 ;0.38) 0.002 -0.10 (-0.26 ; 0.06) 0.20 -0.13 (-0.29 ; 0.03) 0.11 FMI Model 1 0.15 (0.02 ; 0.27) 0.024 -0.15 (-0.28 ; -0.03) 0.017 -0.16 (-0.29 ; -0.03) 0.013 0.23 (0.08 ; 0.37) 0.002 -0.11 (-0.26 ; 0.04) 0.15 -0.09 (-0.24 ; 0.06) 0.22 Model 2 0.15 (0.01 ; 0.28) 0.034 -0.17 (-0.30 ; -0.04) 0.011 -0.18 (-0.31 ; -0.05) 0.006 0.27 (0.12 ; 0.41) < 0.001 -0.16 (-0.32 ; -0.00) 0.047 -0.15 (-0.30 ; 0.01) 0.073 FFMI Model 1 0.07 (-0.06 ; 0.20) 0.27 -0.13 (-0.26 ; -0.001) 0.043 -0.13 (-0.26 ; -0.01) 0.040 0.11 (-0.03 ; 0.26) 0.13 -0.03 (-0.18 ; 0.12) 0.69 -0.05 (-0.20 ; 0.09) 0.48 Model 2 0.01 (-0.12 ; 0.14) 0.89 -0.13 (-0.25 ; -0.001) 0.049 -0.13 (-0.26 ; -0.01) 0.037 0.05 (-0.09 ; 0.19) 0.48 0.06 (-0.09 ; 0.21) 0.44 0.02 (-0.13 ; 0.18) 0.75 ASMI Model 1 0.02 (-0.10 ; 0.15) 0.70 -0.15 (-0.29 ; -0.02) 0.022 -0.15 (-0.28 ; -0.03) 0.017 0.10 (-0.04 ; 0.25) 0.17 -0.01 (-0.16 ; 0.13) 0.85 -0.02 (-0.17 ; 0.13) 0.78 Model 2 -0.05(-0.18 ; 0.09) 0.49 -0.14 (-0.26 ; -0.01) 0.034 -0.15 (-0.27 ; -0.02) 0.022 0.05 (-0.10 ; 0.19) 0.53 0.06 (-0.09 ; 0.22) 0.42 0.05 (-0.10 ; 0.20) 0.53 Model 1: no adjustment. Model 2: adjustment for age, modified Charlson score (excluding age), MMSE and the presence of a caregiver at home. All variables have been centered and scaled. BMI: body mass index, FMI: fat mass index, ASMI: appendicular skeletal muscle mass index, FFMI: Fat free mass index All continuous variables were standardized for analysis. Results should therefore be interpreted as “the concerned body composition parameters grows of β standard deviation units by standard deviation majoration of the concerned biomarker, after adjustment on confounding variables In both sexes, after adjustment for confounding factors, we observed lower fat mass index to be significantly associated with lower Aβ-42/Aβ-40 ratio, reflecting higher amyloid burden (women β = 0.15, p = 0.034; men β = 0.27, p < 0.001). Similarly, higher pTau181 levels were significantly associated with lower fat mass index (women β=-0.17, p = 0.011, men β=-0.16, p = 0.047). There was no association between the Aβ-42/Aβ-40 ratio and muscle mass (fat-free mass index and skeletal muscle index). Sex-related differences in the associations between nutritional status and CSF AD biomarkers were also detected. Lower BMI was significantly associated with a lower Aβ-42/Aβ-40 ratio (reflecting higher amyloid burden) in men (β = 0.23, p = 0.002) but not in women (β = 0.12, p = 0.071). Lower BMI was also associated with higher pTau181 level (reflecting phospho-tau accumulation) in women (β = -0.19, p = 0.003), but not in men (β = -0.10, p = 0.20). In addition, lower fat mass index was associated with higher total tau level (β=-0.18, p = 0.006) in women, but not in men (β=-0.15, p = 0.073). Conversely, lower fat-free mass index (β = − 0.13, p = 0.049) and appendicular skeletal muscle mass index (β = − 0.14, p = 0.034) were associated with higher pTau-181 level and with higher t-tau levels (β = − 0.13, p = 0.037; β = − 0.15, p = 0.022) only in women. We performed a subgroup analysis by diagnosis for the AD, NND, and OND groups, adjusting for age, sex, modified Charlson score, MMSE, and presence of a caregiver at home ( supplementary table 1 and supplementary Fig. 1 ). In the AD subgroup, a lower BMI was associated with higher pTau181 and t-tau levels (respectively β=-0.14, p = 0.024 and β=-0.15, p = 0.011). Lower fat mass index was associated with higher t-tau (β=-0.12, p = 0.032), and low appendicular skeletal muscle mass index was related to higher pTau and t-tau levels (respectively β=-0.10, p = 0.028 and β=-0.11, p = 0.024). No association was observed between biomarkers and body composition in NND patients. In OND patients, a lower BMI was associated with a lower Aβ-42/Aβ-40 ratio (β = 0.33, p = 0.011), and a lower fat mass index was also associated with a lower Aβ-42/Aβ-40 ratio (β = 0.27, p = 0.021) (reflecting higher amyloid burden). 3.4. Clinical factors associated with malnutrition Clinical and social factors associated with malnutrition in the univariate analysis are presented in Supplementary Table 2. In our total cohort, malnourished patients were older (76.5 ± 7.17 years) than non-malnourished ones (74.1 ± 8.21 years, p = 0.0031), and women were more represented in the malnourished group than in the non-malnourished group (66.9% vs 52.6%, p = 0.0059). Median IADL score was lower in malnourished patients (3.0 [2;4]) than in non-malnourished patients (4.0 [2;4] p < 0.001). The mean CCI score was higher in malnourished patients (4.51 ± 1.35) than in non-malnourished individuals (4.16 ± 1.59, p = 0.031), and social isolation was more frequent in malnourished patients (49.6% vs 33.8%, p = 0.0035). The median MMSE score was also lower in malnourished patients (22, [19; 25]) than in their non-malnourished counterparts (24, [20; 27], p = 0.0096). Clinical characteristics associated with malnutrition in the multivariate analysis are reported in Table 3 and Fig. 2 . In the total sample, malnutrition was positively associated with T-tau (OR 1.24, [95% CI 1.005–1.56], p = 0.048). Malnutrition was not associated with Aβ42/Aβ40 ratio or pTau181 after adjustment for age, modified CCI score, MMSE, and the presence of a non-professional caregiver at home. Table 3 Effect of clinical outcomes in the relationship between Alzheimer’s disease CSF biomarkers and malnutrition Total (n = 420) Men (n = 180) Women (n = 240) OR (CI) p OR (CI) p OR (CI) p Ratio Aβ 42/40 Model 1 0.73 (0.56 ; 0.93) 0.018 0.84 (0.53 ; 1.20) 0.39 0.73 (0.54 ; 0.97) 0.035 Model 2 0.87 (0.65 ; 1.11) 0.29 0.92 (0.58 ; 1.33) 0.69 0.81 (0.59 ; 1.11) 0.20 pTau181 Model 1 1.38 (1.13 ; 1.70) 0.002 0.98 (0.69 ; 1.37) 0.92 1.49 (1.14 ; 1.98) 0.004 Model 2 1.21 (0.98 ; 1.51) 0.078 0.85 (0.57 ; 1.24) 0.41 1.41(1.07 ; 1.88) 0.017 t-Tau Model 1 1.42 (1.16 ; 1.75) 0.001 1.01 (0.71 ; 1.41) 0.95 1.53 (1.16 ; 2.04) 0.003 Model 2 1.24 (1.005 ; 1.56) 0.048 0.89 (0.60 ; 1.31) 0.58 1.45 (1.10 ; 1.96) 0.012 Multiple logistic regression based on the biomarker alone (model a), and after adjustment for age, modified Charlson score (excluding age), MMSE, and the presence of a caregiver at home (model b). Analysis on total population were further adjusted on sex All variables were standardized for analysis. Results can be interpreted as xx% change in risk of malnutrition associated with one standard deviation majoration in the biomarker of interest, after adjustment for confounding variable In women, malnutrition was positively associated with pTau181 (OR 1.41 [95% CI 1.07–1.88], p = 0.017) and T-tau (OR 1.45 [95% CI 1.10–1.96], p = 0.012), after adjustment for confounding factors. Despite an association of the Aβ42/Aβ40 ratio with malnutrition in our univariate analysis, this association was no longer significant after adjustment for confounding factors. No association between CSF biomarkers and malnutrition was found in men. Logistic regression models odds ratio adjusted for age, modified CCI score, MMSE score, and presence of a caregiver at home. Global population analysis is further adjusted on sex. 4. Discussion In this study, based on clinical practice, using the most recent diagnosis tools and nutritional status criteria, we described the specific prevalence of malnutrition in these populations. In the whole cohort, we found in both sexes that the fat mass index was positively correlated with amyloid ratio CSF levels and inversely correlated to pTau181 levels. In summary, a low fat mass index is associated with high amyloid and pTau181 brain accumulations. In AD patients, a lower fat mass index was inversely correlated with CSF T-tau levels, reflecting brain T-tau accumulation. In women, the muscle mass was inversely associated with pTau181 and t-tau. pTau181 and t-tau were independently associated with malnutrition after adjustment for confounding factors. Finally, regarding the other factors associated with malnutrition, we observed that age, sex, social isolation, and comorbidity burden were all associated with malnutrition in the univariate analysis. With regards to our first objective, the already reported prevalence of malnutrition in cognitively impaired individuals, as measured by the MNA questionnaire, varies widely from 2.9% to 17.4% according to previous studies [35–37]. In our results, the prevalence of malnutrition and low muscle mass was much higher, including a significant sex-related disparity in disfavor of women. Indeed, we used the GLIM criteria, which include age-specific BMI thresholds, assessment of muscle mass and weight loss over an extended period. These criteria allow for a more specific and objective determination of the nutritional status, and could explain for the discordant findings between previous studies and our results. As illustrated by Ozer et al. , in a population without cognitive decline, 32.2% of older adults without dementia were malnourished using the GLIM criteria, whereas the MNA and MNA-SF only identified malnutrition risk in 12.7% and 13.1% of subjects [38]. MNA primarily identifies the risk for malnutrition [17,21,24,35,37] without any confirmation and independently of age and sex; furthermore, the reliability of the questionnaire could be inferior in patients with cognitive decline, altogether potentially leading to an underestimated malnutrition. Overall, these results underscore the important need to implement rigorous nutritional assessment and management strategies, aiming at detecting, understanding, preventing, and treating malnutrition in this population. Our second objective was to investigate the relationships between AD CSF biomarkers and body composition parameters. Previously, a cross-sectional study from Doorduijn et al. using the MNA score in a prospective cohort from daily practice of more than 500 AD patients and controls, reported an inverse association between both pTau181 and t-tau and fat-free mass, but no association with the Aβ42 peptide. However, analyses were not stratified by sex in this study, but were only adjusted for sex.[24]. In our study, we observed similar results, but only in women associated with an inverse association of pTau 181 and t-tau with muscle mass parameters. We mainly found an association of amyloid CSF biomarker (amyloid accumulation) and CSF pTau181 (pTau accumulation) with fat mass, which was not previously described in relation to CSF biomarkers, in mild cognitive impairment. In AD patients, a lower fat mass index was linked to total tau brain accumulation. These results imply that in neurocognitive disorders, low levels of body fat are associated with amyloid and pTau 181 brain accumulations and that in AD patients, a lower fat mass index was linked to increased neurodegeneration. Two studies in the general population have specifically examined the relationship between fat mass or muscle mass and plasma AD biomarkers and found different results. De Crom et al. showed that fat mass was positively associated with t-tau [39], while Hermesdorf et al . found fat mass was inversely associated with β-amyloid 42 levels and positively associated with Aβ-40/Aβ-42 ratio [40]. These two studies focused on the general population without cognitive impairment, younger age, and higher BMI, which can explain, at least partially, the difference with our results. Moreover, unlike CSF biomarkers, the relationships between plasma biomarkers and body composition may be confounded by variations in volume of distribution, making it difficult to establish a true pathophysiological connection [41]. Interestingly, all the reported links between AD biomarkers and nutritional status suggest a possible common pathophysiological hypothesis. We found a marked association between amyloid, tau biomarkers and fat mass, both in men and women, suggesting that AD-related pathology and neurodegeneration may be linked to a preferential loss of fat mass relative to muscle mass. Leptin deficit may be a cause of this association [42]. Leptin is mainly produced by adipocytes and is involved in the regulation of appetite and energy expenditure; it also plays a neuroprotective role, stimulating neurogenesis and participating in neurocognitive functions such as memory or learning [43]. Ishii et al. showed that AD mouse models exhibited reduced fat mass and lower leptin levels compared with wild-type controls. In response to these low leptin levels, AD mice revealed low levels of neuropeptide Y (NPY), an orexigenic peptide, and not high levels as expected [44]. In another AD mouse model, Robison et al . suggested that impaired hypothalamic signaling could explain the inadequate response to low leptin levels observed in the context of AD, thus favoring weight and fat mass loss [45]. Data from these preclinical models provide insights into potential mechanisms underlying our findings, highlighting the association of tau and amyloid CSF biomarkers with fat mass loss in both sexes. In AD patients, the links between neurodegeneration and low fat mass index could be associated with reduced leptin neuroprotection, as shown in our previous studies [42,46]. Moreover, our diagnostic subgroup results support a specific link between brain amyloid deposition and fat mass reduction, even in OND patients. Nevertheless, further studies investigating markers of neuroinflammation or neurodegeneration in relation to body composition changes across different neurodegenerative diseases are needed to better clarify the nature of this association. Another key finding of this study is the association between malnutrition and CSF AD biomarkers in women. The sex difference observed in our study is consistent with preclinical studies in this field. Lopez-Gamboro et al. reported that neuroinflammation was associated with reduced food intake and subsequent weight loss in female mutant mice [47], in line with our results. However, in humans, other socio-environmental factors can be involved. Notably, women experienced more social isolation than men in our study, which could contribute to malnutrition. The strengths of our study include its multicenter design, involving both geriatric and neurological centers, allowing the inclusion of patients across a broad age range. We also specifically performed sex-stratified analyses, enabling sex specificities that could lead to adapted care according to sex. However, several limitations should be acknowledged. The absence of longitudinal data and missing information on prior weight loss likely led to an underestimation of the prevalence of malnutrition. Given the hypothesis of progressive, gradual weight loss due to reduced appetite in neurodegenerative diseases, cross-sectional measurements of fat and muscle mass do not capture individuals’ changes in body composition during the disease. Moreover, despite exclusion criteria and adjustment for confounders, malnutrition is often multifactorial, and it remains challenging to account for all relevant factors, such as mood disorders, hospitalizations and environmental or caregiving conditions. Finally, limited statistical power may restrict the generalizability of our findings in men. 5. Conclusion Our study highlights a close association between malnutrition, loss of fat and muscle mass, and AD CSF biomarkers, which could suggest a common pathophysiological link. Further longitudinal investigations, including the evaluation of appetite-regulating hormones (such as glucagon-like peptide-1 and others), are needed. In parallel, preventive strategies aimed at maintaining adequate nutritional status—through balanced diets, early screening for malnutrition, and tailored nutritional interventions—may help limit or delay cognitive decline and neurodegeneration potentially associated with malnutrition. Declarations Ethics approval All participants participated in the BioCogBank protocol NCT06244875), and gave written consent for CSF and plasma collection for further analysis. They also provided consent for the use of their clinical data and the results of their CSF analyses. This study was approved by local and national Ethics Committees ("Comité d'évaluation et d'Ethique pour la recherche Paris Nord" and by the "Commission Nationale Informatique et Libertés" (CNIL)). Consent for publication Not applicable Competing interest None Funding None Author Contribution Karl Götze: Conceptualization, Data curation, formal analysis, methodology, writing – original draft, writing – review and editingAgathe Vrillon: Data curation, writing – original draft, writing – review and editingManuel Sanchez : Methodology, writing – review and editingInès Petit-Damico : Data curation, writing – original draft, writing – review and editingElodie Bouaziz-Amar : formal analysis, writing – review and editingSophie Lacaille :Data curation, writing – review and editingClaire Hourrègue : Data curation, writing – review and editingEmmanuel Cognat : Data curation, writing – review and editingJacques Hugon : Data curation, writing – review and editingThéodore Decaix : Data curation, writing – review and editingJulien Dumurgier : Data curation, writing – review and editingAgathe Raynaud-Simon : Methodology, writing – review and editingMatthieu Lilamand : Conceptualization, writing – review and editingClaire Paquet : Conceptualization, writing – original draft, writing – review and editing Acknowledgement None Data Availability The dataset used and/or analysed during the current study are available from the corresponding author on reasonable request References G S, M DR, A C, Em I, E M. 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08:47:30","extension":"html","order_by":9,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":115126,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7980996/v1/410f95b6231e53e6367ad257.html"},{"id":95806679,"identity":"38f3a165-9552-460e-8c5a-fb8ad8c8ee3b","added_by":"auto","created_at":"2025-11-13 08:47:46","extension":"jpeg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":202005,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cem\u003eBody composition and AD CSF biomarkers in women and men.\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003eA: Relation between Aβ42/40 ratio and body composition in women\u003c/p\u003e\n\u003cp\u003eB: Relation between pTau-181 and body composition in women\u003c/p\u003e\n\u003cp\u003eC: Relation between Total Tau and body composition in women\u003c/p\u003e\n\u003cp\u003eD: Relation between Aβ42/40 ratio and body composition in men\u003c/p\u003e\n\u003cp\u003eE: Relation between pTau-181 and body composition in men\u003c/p\u003e\n\u003cp\u003eF: Relation between Total Tau and body composition in men\u003c/p\u003e\n\u003cp\u003eBMI: body mass index, FMI: fat mass index, ASMI: skeletal muscle mass index, FFMI: Fat free mass index\u003c/p\u003e","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7980996/v1/cb79ad187227c34c8ba0fbaf.jpeg"},{"id":95806728,"identity":"6fe8fd7e-44e9-4f05-a284-72f1870f3007","added_by":"auto","created_at":"2025-11-13 08:47:50","extension":"jpeg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":106266,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cem\u003eEffect of clinical outcomes and sex on the relationship between Alzheimer’s disease CSF biomarkers and malnutrition.\u003c/em\u003e\u003c/p\u003e","description":"","filename":"floatimage2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7980996/v1/3b61a3cc9329be338125ec58.jpeg"},{"id":101151799,"identity":"7f5af700-af96-4fc0-99c3-5626dd77a7a9","added_by":"auto","created_at":"2026-01-26 16:05:45","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1369773,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7980996/v1/ce34c128-d609-4dc7-8e09-e9127377dfdc.pdf"},{"id":95806565,"identity":"7af8004a-cda5-4da3-ae50-6a4b09b0fd15","added_by":"auto","created_at":"2025-11-13 08:47:38","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":254437,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementarymaterialsBodycomp.docx","url":"https://assets-eu.researchsquare.com/files/rs-7980996/v1/436c8c2734e2db923a7f0cfb.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Negative association of fat mass index and Alzheimer’s disease cerebrospinal fluid biomarkers in cognitively impaired patients","fulltext":[{"header":"1. Background","content":"\u003cp\u003eA large body of evidence links nutritional status and body composition with the pathophysiology of neurodegenerative disorders, specifically in Alzheimer\u0026rsquo;s disease (AD). Chronic inflammation, altered energy metabolism, and dysregulation of appetite-related hormones were suggested to explain the association between weight loss and AD [1].\u003c/p\u003e\u003cp\u003eIn addition, reciprocal relationships between nutritional status and cognitive decline were consistently reported. Notably, unintentional weight loss or lower Body Mass Index (BMI) were described several years before the onset of clinically detectable neurocognitive disorders, as evidenced by large-scale prospective cohort studies [2\u0026ndash;5]. In AD, accelerated cognitive decline was also reported in individuals with notable weight loss during the first year of follow-up [6] or with lower BMI at baseline [7]. Similar results were found in a general population cohort of 3035 patients, in which higher baseline BMI and smaller weight loss were associated with a slower rate of cognitive decline [8]. These findings are further supported by the results of the Dominantly Inherited Alzheimer Network (DIAN) study[9], in which asymptomatic carriers began to lose weight approximately 11 years earlier than asymptomatic non-carriers. Moreover, in the same cohort, lower BMI in the preclinical stage was associated with earlier symptom onset and higher amyloid burden.\u003c/p\u003e\u003cp\u003eThe use of bioelectrical impedance analysis assesses the body composition by notably estimating lean mass, muscle mass, and fat mass. Indeed, low muscle mass is a phenotypic criterion for sarcopenia and for malnutrition[10] [11]. Findings about the association of incident dementia and body composition are inconsistent in the current literature. Two general population studies based on the UK Biobank cohort reported an inverse association between incident dementia and the fat-to-muscle ratio [12,13]. Other studies reported incident dementia to be associated with lower lean mass [14]. In contrast, other results found no association between incident dementia and body composition [15] or fat mass [14].\u003c/p\u003e\u003cp\u003eA few studies examined body composition specifically in AD. They reported lower lean mass in AD patients compared to controls [16], a higher prevalence of sarcopenia in moderate versus mild AD [17], and in AD patients compared with controls [18,19]. Interestingly, lower fat mass and lower lean mass were also associated with lower cortical thickness in AD-affected brain regions [20,21]. However, in those studies, AD diagnoses were made according to former AD diagnosis criteria (Mc Kahn 1984) without AD CSF biomarkers.\u003c/p\u003e\u003cp\u003eUsing CSF biomarker evidence [22,23], a publication by Doorduijn et al. observed a significant inverse association between phosphorylated Tau on threonine 181 (pTau181) and total-Tau (t-tau) with fat-free mass index, but no association with amyloid biomarkers [24] in 558 participants, including AD, mild cognitive impairment, and controls. They did not explore the sex differences.\u003c/p\u003e\u003cp\u003eOverall, the available evidence on the relationship between cognitive decline, nutritional status, and biological biomarkers of AD remains limited, particularly regarding to sex differences and possible confounding factors.\u003c/p\u003e\u003cp\u003eIn the present study, we aimed to examine the relationships between AD cerebrospinal fluid (CSF) biomarkers, nutritional status, and body composition. Our objectives were: (1) to investigate the prevalence of malnutrition and low muscle mass in cognitive disorders; (2) to examine the association between AD CSF biomarkers and body composition, possibly suggesting a potential pathophysiological link, and (3) to identify the factors associated with malnutrition in these patients.\u003c/p\u003e"},{"header":"2. Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\u003ch2\u003e2.1. Study Design and Participants\u003c/h2\u003e\u003cp\u003eWe performed a prospective multicentric study including patients evaluated for neurocognitive disorders who underwent CSF analysis for AD biomarker testing. Patients were enrolled from January 2022 to July 2025 at three centers at the Universit\u0026eacute; Paris Cit\u0026eacute; GHU AP-HP Nord, Paris, France, including Lariboisi\u0026egrave;re Hospital, a tertiary memory clinic, and two geriatric day-care hospitals: Bretonneau and Fernand Widal.\u003c/p\u003e\u003cdiv id=\"Sec4\" class=\"Section3\"\u003e\u003ch2\u003e2.1.1. Inclusion and Exclusion Criteria\u003c/h2\u003e\u003cp\u003eAll patients who had a lumbar puncture for CSF AD biomarker measurement and signed a written informed consent for the BioCogBank study (see \u0026sect;\u0026nbsp;2.6) were included. Patients with conditions likely to cause inflammation, such as active cancer, infectious diseases, and chronic inflammatory diseases, and any chronic gastrointestinal condition, that adversely impact food assimilation or absorption, were excluded.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec5\" class=\"Section3\"\u003e\u003ch2\u003e2.1.2. Cognitive disease diagnostic assessment\u003c/h2\u003e\u003cp\u003eIn each center, patients were evaluated by multidisciplinary teams composed of geriatricians, neurologists, neuropsychologists, biochemists, and neuroradiologists. Diagnoses were established after multidisciplinary team assessment including examination of CSF biomarkers, MRI and fluorodeoxyglucose positron emission tomography (PET) imaging. AD was diagnosed using the most recent diagnostic criteria for AD [25]. Other neurodegenerative diseases (OND) included dementia with Lewy Bodies (DLB) [26], frontotemporal dementia (FTD) [27], supranuclear palsy [28], corticobasal degeneration [29] or patients with cognitive decline and brain atrophy or hypometabolism on PET considered to have a neurodegenerative process after multidisciplinary case review. Patients were classified as having a non-neurodegenerative disorder (NND) if they did not meet criteria for neurodegenerative diseases (as defined above) and were considered as non-neurodegenerative by the clinician in charge, after multidisciplinary team assessment, including evaluation of CSF biomarkers, MRI, PET imaging, and cognitive follow-up. Patients without a confirmed diagnosis were classified as \u0026ldquo;unclassified dementia\u0026rdquo; and were excluded from the diagnostic-subgroup analysis.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec6\" class=\"Section2\"\u003e\u003ch2\u003e2.2. CSF Biomarkers analysis\u003c/h2\u003e\u003cp\u003eAfter lumbar puncture, CSF samples were centrifuged at 1000\u003cem\u003eg\u003c/em\u003e for 10 min at 4\u0026deg;C within 2 h of collection and then aliquoted into 0.5 mL polypropylene tubes before being stored at \u0026minus;\u0026thinsp;80\u0026deg;C for further analysis.\u003c/p\u003e\u003cp\u003eCSF t-tau, pTau181, and Aβ42 measurements were conducted in the Biochemistry Unit of Lariboisi\u0026egrave;re Hospital using Elecsys\u0026reg; assays on the cobas e601 analyser (Roche Diagnostics). CSF Aβ40 measurement was performed using an Innotest ELISA assay (Fujirebio, Ghent, Belgium). Patients were considered as amyloid positive (A+) according to Aβ 42/40 ratio below 0.083, tau positive (T+) if pTau181 level was over 22 pg/mL and neurodegeneration positive (N+) if t-tau was over 220 pg/mL.\u003c/p\u003e\u003cp\u003eLumbar puncture for AD biomarker measurements was performed within six months of the body composition assessment (mean delay: 0.55\u0026thinsp;\u0026plusmn;\u0026thinsp;1.94 months)\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e\u003ch2\u003e2.3. Nutritional status determination and bioelectrical impedance measurements\u003c/h2\u003e\u003cp\u003eAnthropometric measurements (weight and height) were obtained using standardized equipment. Multifrequency bioelectrical impedance analysis (BIA) was performed within six months before or after the lumbar puncture, using the InBody S10\u0026reg;. The data collected on fat mass, fat-free mass, and appendicular skeletal muscle were used to determine:\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eFat mass index: Fat mass (kg) / height (m\u0026sup2;)\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eFat-free mass index: Fat-free mass (kg) / height(m\u0026sup2;)\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eAppendicular skeletal muscle mass (ASM, kg)\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eAppendicular skeletal muscle mass index: appendicular skeletal muscle (kg)/ height (m\u0026sup2;)\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003cp\u003eNutritional status, classified as no malnutrition, moderate malnutrition or severe malnutrition, was determined according to the 2019 Global Leadership Initiative on Malnutrition (GLIM) [10], requiring the combination of at least one phenotypic criteria and at least one aetiologic criteria. The phenotypic criteria are low BMI (\u0026lt;\u0026thinsp;20 for patients under 70 years old and \u0026lt;\u0026thinsp;22 for patients aged 70 and over), weight loss\u0026thinsp;\u0026gt;\u0026thinsp;10% or low muscle mass. Low muscle mass was determined using the sex-specific thresholds of the revised European consensus on definition and diagnosis of sarcopenia, considering appendicular skeletal muscle mass\u0026thinsp;\u0026lt;\u0026thinsp;20 kg in men or 15 kg in women and appendicular skeletal muscle mass index\u0026thinsp;\u0026lt;\u0026thinsp;7 kg/m\u0026sup2; in men or 5.5 kg/m\u0026sup2; in women [30]. The aetiologic criteria for malnutrition were reduced appetite (using the Mini-Nutritional Assessment short form specific item), chronic diseases and major neurocognitive disorders. Based on blood tests, patients with biologically detectable inflammation or malabsorption were excluded.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\u003ch2\u003e2.4. Other Variables\u003c/h2\u003e\u003cp\u003eAdditional variables, potentially affecting nutritional status and body composition, were also recorded:\u003c/p\u003e\u003cp\u003e\u003cul\u003e\u003cli\u003e\u003cp\u003eFunctional status assessed by the 4-item Instrumental Activities of Daily Living (IADL) score [31] and the Activities of Daily Living (ADL) score [32]\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eHome assistance evaluated with the presence of professional help at home (yes/no), and the presence of a relative living at home with the patient (yes/no)\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eComorbidities assessed using the Charlson Comorbidity Index (CCI) score [33]. The modified CCI was calculated excluding the age component to assess the comorbidity burden independently of age\u003c/p\u003e\u003c/li\u003e\u003cli\u003e\u003cp\u003eCognitive impairment severity was measured using the Mini-Mental State Examination (MMSE) score [34].\u003c/p\u003e\u003c/li\u003e\u003c/ul\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec9\" class=\"Section2\"\u003e\u003ch2\u003e2.5. Statistical Analyses\u003c/h2\u003e\u003cdiv id=\"Sec10\" class=\"Section3\"\u003e\u003ch2\u003e2.5.1. Descriptive Analyses\u003c/h2\u003e\u003cp\u003eDue to substantial sex-related differences in body composition parameters, analyses were stratified by sex. Based on a hypothesis of a moderate correlation (r=-0.2) between CSF Aβ 42/40 ratio and fat mass[24] 193 patients are needed to reach a power of 80% with an alpha-risk at 5% (using Fisher Z transformation method) in each sex, meaning a total of 386 patients needed.\u003c/p\u003e\u003cp\u003eCategorical variables were compared using the χ\u0026sup2; test, or Fisher's exact test as appropriate. Continuous variables were compared using Student's t-test for variables following a parametric distribution and presented using mean\u0026thinsp;\u0026plusmn;\u0026thinsp;standard deviation (SD). Variables of non-parametric distribution, of MMSE, ADL, and IADL scores were compared using the Wilcoxon test and presented using the median (1st ; 3rd quartiles).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec11\" class=\"Section3\"\u003e\u003ch2\u003e2.5.2. Multivariate Analyses\u003c/h2\u003e\u003cp\u003eMissing data for covariates (age, modified CCI, presence of a caregiver at home) were treated by multiple imputation. All numerical variables were centered and scaled considering the mean and standard deviation of the population, both for linear and logistic regressions. Linear regression models examining BMI, fat mass index, fat-free mass index, and appendicular skeletal muscle mass index on the one hand, and CSF Aβ-42/Aβ-40 ratio, pTau181, and t-tau on the other hand, were adjusted for age, modified CCI score, MMSE score, and the presence of a caregiver at home, as clinically relevant variables, linked to malnutrition. Diagnostic subgroup analyses were performed to evaluate the relationship between AD CSF biomarkers and body composition metrics in each diagnosis group. These analyses were also adjusted for sex, age, modified CCI score, MMSE score, and presence of a caregiver at home, but not stratified by sex.\u003c/p\u003e\u003cp\u003eThe association between malnutrition and CSF AD biomarkers was assessed using multiple logistic regression, adjusting for age, modified CCI score, MMSE score, and presence of a caregiver at home. Analysis across the global population was also adjusted for sex. All multivariate analyses were stratified by sex to account for sex-specific differences in fat mass and lean mass.\u003c/p\u003e\u003cp\u003eIn all analyses, the type I error risk α was set at 0.05 and the power at 80%. Statistical analyses were performed using the R software (Version 4.3.1).\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\u003ch2\u003e2.6. Ethics\u003c/h2\u003e\u003cp\u003eAll data and samples analyzed were part of the BioCogBank study (NCT06244875). All participants gave written informed consent. This study was approved by the \u0026ldquo;Comit\u0026eacute; de Protection des Personnes Est III\u0026rdquo; (2023-A01413-42, 23-10-02) and by the \"Commission Nationale Informatique et Libert\u0026eacute;s\" (CNIL)\u003c/p\u003e\u003c/div\u003e"},{"header":"3. Results","content":"\u003cdiv id=\"Sec14\" class=\"Section2\"\u003e\u003ch2\u003e3.1. Population\u0026rsquo;s characteristics\u003c/h2\u003e\u003cp\u003ePatients\u0026rsquo; characteristics are presented in \u003cb\u003eTable\u0026nbsp;1\u003c/b\u003e. A total of 420 participants were included: 240 (57.1%) women and 180 (42.9%) men. Our study sample mainly comprised mild-stage neurocognitive disorders, with a median MMSE of 23 [interquartile range 19; 26]. The most frequent diagnosis was AD (57.4%), followed by NND (13.6%), OND (12.1%), and 16.9% of the population had unclassified dementia at the time of the study. Women had a lower comorbidity index (mean CCI score 4.09\u0026thinsp;\u0026plusmn;\u0026thinsp;1.38 vs 4.51\u0026thinsp;\u0026plusmn;\u0026thinsp;1.66 for men, p\u0026thinsp;=\u0026thinsp;0.0065) and lived alone more often than men (52.1% vs 21.1%, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec15\" class=\"Section2\"\u003e\u003ch2\u003e3.2. Prevalence of malnutrition and muscle mass loss in neurocognitive disorders\u003c/h2\u003e\u003cp\u003eRegarding nutritional assessment, malnutrition was diagnosed in 31.7% of the patients, including 15.5% with severe malnutrition (\u003cb\u003eTable\u0026nbsp;1\u003c/b\u003e). Low muscle mass was observed in 18.1% of the patients, low BMI in 27.1% and \u0026gt;\u0026thinsp;10% weight loss in 11.7%.\u003c/p\u003e\u003cp\u003eWomen were more frequently malnourished than men (37.1% vs 24.4%, p\u0026thinsp;=\u0026thinsp;0.0059). The prevalence of severe malnutrition was higher in women (20.8%) than in men (8.3%, p\u0026thinsp;=\u0026thinsp;0.0034). In addition, women also had a more frequent lower muscle mass (22.9%) than men (11.7%), p\u0026thinsp;=\u0026thinsp;0.0033.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec16\" class=\"Section2\"\u003e\u003ch2\u003e3.3. Association between CSF AD biomarkers and body composition\u003c/h2\u003e\u003cp\u003eAll data are presented in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e2\u003c/span\u003e \u003cb\u003eand\u003c/b\u003e Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eAssociation of Aβ-42/Aβ-40 ratio, pTau 181, and total Tau with body composition according to sex.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"14\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c9\" colnum=\"9\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c10\" colnum=\"10\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c11\" colnum=\"11\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c12\" colnum=\"12\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c13\" colnum=\"13\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c14\" colnum=\"14\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colspan=\"7\" nameend=\"c8\" namest=\"c2\"\u003e\u003cp\u003eWomen\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colspan=\"6\" nameend=\"c14\" namest=\"c9\"\u003e\u003cp\u003eMen\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\"\u003e\u003cp\u003eAβ42/40 ratio\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c4\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c5\"\u003e\u003cp\u003epTau 181\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c6\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c7\"\u003e\u003cp\u003eTotal Tau\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c8\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAβ42/Aβ40 ratio\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c10\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c11\"\u003e\u003cp\u003epTau 181\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c12\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c13\"\u003e\u003cp\u003eTotal Tau\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c14\"\u003e\u0026nbsp;\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e\u003cem\u003eEstimate (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e\u003cem\u003eEstimate (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e\u003cem\u003eEstimate (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e\u003cem\u003eEstimate (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e\u003cem\u003eEstimate (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e\u003cem\u003eEstimate (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eBMI\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 1\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.15 (0.03\u0026nbsp;;\u0026nbsp;0.28)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.018\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.19 (-0.31 ; -0.06)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.004\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.19 (-0.32 ; -0.07)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.003\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.24 (0.09\u0026nbsp;;\u0026nbsp;0.38)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e\u003cb\u003e0.001\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e-0.11 (-0.26 ; 0.03)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.12\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e-0.13 (-0.28\u0026nbsp;;\u0026nbsp;-0.01)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.073\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 2\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.12 (-0.01\u0026nbsp;; 0.26)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.071\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.19 (-0.32 ; -0.07)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.003\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.21 (-0.33 ; -0.08)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.002\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.23 (0.08\u0026nbsp;;0.38)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e\u003cb\u003e0.002\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e-0.10 (-0.26 ; 0.06)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.20\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e-0.13 (-0.29\u0026nbsp;;\u0026nbsp;0.03)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.11\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eFMI\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 1\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.15 (0.02 ; 0.27)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.024\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.15 (-0.28\u0026nbsp;;\u0026nbsp;-0.03)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.017\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.16 (-0.29 ; -0.03)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.013\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.23 (0.08 ; 0.37)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e\u003cb\u003e0.002\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e-0.11 (-0.26\u0026nbsp;;\u0026nbsp;0.04)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.15\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e-0.09 (-0.24\u0026nbsp;;\u0026nbsp;0.06)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.22\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 2\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.15 (0.01 ; 0.28)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.034\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.17 (-0.30\u0026nbsp;; -0.04)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.011\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.18 (-0.31\u0026nbsp;;\u0026nbsp;-0.05)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.006\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.27 (0.12 ; 0.41)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e\u003cb\u003e\u0026lt;\u0026thinsp;0.001\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e-0.16 (-0.32\u0026nbsp;; -0.00)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e\u003cb\u003e0.047\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e-0.15 (-0.30 ; 0.01)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.073\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eFFMI\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 1\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.07 (-0.06 ; 0.20)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.27\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.13 (-0.26\u0026nbsp;;\u0026nbsp;-0.001)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.043\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.13 (-0.26\u0026nbsp;;\u0026nbsp;-0.01)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.040\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.11 (-0.03 ; 0.26)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e0.13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e-0.03 (-0.18\u0026nbsp;;\u0026nbsp;0.12)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.69\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e-0.05 (-0.20\u0026nbsp;; 0.09)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.48\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 2\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.01 (-0.12 ; 0.14)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.89\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.13 (-0.25\u0026nbsp;; -0.001)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.049\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.13 (-0.26\u0026nbsp;;\u0026nbsp;-0.01)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.037\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.05 (-0.09 ; 0.19)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e0.48\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e0.06 (-0.09\u0026nbsp;; 0.21)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.44\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e0.02 (-0.13\u0026nbsp;;\u0026nbsp;0.18)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.75\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eASMI\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 1\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.02 (-0.10 ; 0.15)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.70\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.15 (-0.29\u0026nbsp;;\u0026nbsp;-0.02)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.022\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.15 (-0.28\u0026nbsp;;\u0026nbsp;-0.03)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.017\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.10 (-0.04 ; 0.25)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e0.17\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e-0.01 (-0.16\u0026nbsp;;\u0026nbsp;0.13)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.85\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e-0.02 (-0.17\u0026nbsp;;\u0026nbsp;0.13)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.78\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eModel 2\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e-0.05(-0.18 ; 0.09)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.49\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-0.14 (-0.26\u0026nbsp;;\u0026nbsp;-0.01)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e0.034\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e-0.15 (-0.27 ;\u0026nbsp;-0.02)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.022\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003e0.05 (-0.10 ; 0.19)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003e0.53\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003e0.06 (-0.09\u0026nbsp;;\u0026nbsp;0.22)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003e0.42\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003e0.05 (-0.10\u0026nbsp;;\u0026nbsp;0.20)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003e0.53\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003ctfoot\u003e\u003ctr\u003e\u003ctd colspan=\"14\"\u003eModel 1: no adjustment. Model 2: adjustment for age, modified Charlson score (excluding age), MMSE and the presence of a caregiver at home. All variables have been centered and scaled. BMI: body mass index, FMI: fat mass index, ASMI: appendicular skeletal muscle mass index, FFMI: Fat free mass index\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd colspan=\"14\"\u003e\u003cem\u003eAll continuous variables were standardized for analysis. Results should therefore be interpreted as \u0026ldquo;the concerned body composition parameters grows of β standard deviation units by standard deviation majoration of the concerned biomarker, after adjustment on confounding variables\u003c/em\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tfoot\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eIn both sexes, after adjustment for confounding factors, we observed lower fat mass index to be significantly associated with lower Aβ-42/Aβ-40 ratio, reflecting higher amyloid burden (women β\u0026thinsp;=\u0026thinsp;0.15, p\u0026thinsp;=\u0026thinsp;0.034; men β\u0026thinsp;=\u0026thinsp;0.27, p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). Similarly, higher pTau181 levels were significantly associated with lower fat mass index (women β=-0.17, p\u0026thinsp;=\u0026thinsp;0.011, men β=-0.16, p\u0026thinsp;=\u0026thinsp;0.047). There was no association between the Aβ-42/Aβ-40 ratio and muscle mass (fat-free mass index and skeletal muscle index).\u003c/p\u003e\u003cp\u003eSex-related differences in the associations between nutritional status and CSF AD biomarkers were also detected. Lower BMI was significantly associated with a lower Aβ-42/Aβ-40 ratio (reflecting higher amyloid burden) in men (β\u0026thinsp;=\u0026thinsp;0.23, p\u0026thinsp;=\u0026thinsp;0.002) but not in women (β\u0026thinsp;=\u0026thinsp;0.12, p\u0026thinsp;=\u0026thinsp;0.071). Lower BMI was also associated with higher pTau181 level (reflecting phospho-tau accumulation) in women (β = -0.19, p\u0026thinsp;=\u0026thinsp;0.003), but not in men (β = -0.10, p\u0026thinsp;=\u0026thinsp;0.20). In addition, lower fat mass index was associated with higher total tau level (β=-0.18, p\u0026thinsp;=\u0026thinsp;0.006) in women, but not in men (β=-0.15, p\u0026thinsp;=\u0026thinsp;0.073). Conversely, lower fat-free mass index (β = \u0026minus;\u0026thinsp;0.13, p\u0026thinsp;=\u0026thinsp;0.049) and appendicular skeletal muscle mass index (β = \u0026minus;\u0026thinsp;0.14, p\u0026thinsp;=\u0026thinsp;0.034) were associated with higher pTau-181 level and with higher t-tau levels (β = \u0026minus;\u0026thinsp;0.13, p\u0026thinsp;=\u0026thinsp;0.037; β = \u0026minus;\u0026thinsp;0.15, p\u0026thinsp;=\u0026thinsp;0.022) only in women.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eWe performed a subgroup analysis by diagnosis for the AD, NND, and OND groups, adjusting for age, sex, modified Charlson score, MMSE, and presence of a caregiver at home (\u003cb\u003esupplementary table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003e1\u003c/span\u003e\u003c/b\u003e and \u003cb\u003esupplementary Fig.\u0026nbsp;1\u003c/b\u003e). In the AD subgroup, a lower BMI was associated with higher pTau181 and t-tau levels (respectively β=-0.14, p\u0026thinsp;=\u0026thinsp;0.024 and β=-0.15, p\u0026thinsp;=\u0026thinsp;0.011). Lower fat mass index was associated with higher t-tau (β=-0.12, p\u0026thinsp;=\u0026thinsp;0.032), and low appendicular skeletal muscle mass index was related to higher pTau and t-tau levels (respectively β=-0.10, p\u0026thinsp;=\u0026thinsp;0.028 and β=-0.11, p\u0026thinsp;=\u0026thinsp;0.024).\u003c/p\u003e\u003cp\u003eNo association was observed between biomarkers and body composition in NND patients. In OND patients, a lower BMI was associated with a lower Aβ-42/Aβ-40 ratio (β\u0026thinsp;=\u0026thinsp;0.33, p\u0026thinsp;=\u0026thinsp;0.011), and a lower fat mass index was also associated with a lower Aβ-42/Aβ-40 ratio (β\u0026thinsp;=\u0026thinsp;0.27, p\u0026thinsp;=\u0026thinsp;0.021) (reflecting higher amyloid burden).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec17\" class=\"Section2\"\u003e\u003ch2\u003e3.4. Clinical factors associated with malnutrition\u003c/h2\u003e\u003cp\u003eClinical and social factors associated with malnutrition in the univariate analysis are presented in \u003cb\u003eSupplementary Table\u0026nbsp;2.\u003c/b\u003e In our total cohort, malnourished patients were older (76.5\u0026thinsp;\u0026plusmn;\u0026thinsp;7.17 years) than non-malnourished ones (74.1\u0026thinsp;\u0026plusmn;\u0026thinsp;8.21 years, p\u0026thinsp;=\u0026thinsp;0.0031), and women were more represented in the malnourished group than in the non-malnourished group (66.9% vs 52.6%, p\u0026thinsp;=\u0026thinsp;0.0059). Median IADL score was lower in malnourished patients (3.0 [2;4]) than in non-malnourished patients (4.0 [2;4] p\u0026thinsp;\u0026lt;\u0026thinsp;0.001).\u003c/p\u003e\u003cp\u003eThe mean CCI score was higher in malnourished patients (4.51\u0026thinsp;\u0026plusmn;\u0026thinsp;1.35) than in non-malnourished individuals (4.16\u0026thinsp;\u0026plusmn;\u0026thinsp;1.59, p\u0026thinsp;=\u0026thinsp;0.031), and social isolation was more frequent in malnourished patients (49.6% vs 33.8%, p\u0026thinsp;=\u0026thinsp;0.0035). The median MMSE score was also lower in malnourished patients (22, [19; 25]) than in their non-malnourished counterparts (24, [20; 27], p\u0026thinsp;=\u0026thinsp;0.0096).\u003c/p\u003e\u003cp\u003eClinical characteristics associated with malnutrition in the multivariate analysis are reported in Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e3\u003c/span\u003e \u003cb\u003eand\u003c/b\u003e Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e. In the total sample, malnutrition was positively associated with T-tau (OR 1.24, [95% CI 1.005\u0026ndash;1.56], p\u0026thinsp;=\u0026thinsp;0.048). Malnutrition was not associated with Aβ42/Aβ40 ratio or pTau181 after adjustment for age, modified CCI score, MMSE, and the presence of a non-professional caregiver at home.\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003e\u003cem\u003eEffect of clinical outcomes in the relationship between Alzheimer\u0026rsquo;s disease CSF biomarkers and malnutrition\u003c/em\u003e\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"8\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/th\u003e\u003cth align=\"left\" colspan=\"2\" nameend=\"c4\" namest=\"c3\"\u003e\u003cp\u003eTotal (n\u0026thinsp;=\u0026thinsp;420)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colspan=\"2\" nameend=\"c6\" namest=\"c5\"\u003e\u003cp\u003eMen (n\u0026thinsp;=\u0026thinsp;180)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colspan=\"2\" nameend=\"c8\" namest=\"c7\"\u003e\u003cp\u003eWomen (n\u0026thinsp;=\u0026thinsp;240)\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u0026nbsp;\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e\u003cem\u003eOR (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e\u003cem\u003eOR (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e\u003cem\u003eOR (CI)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cem\u003ep\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eRatio Aβ 42/40\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eModel 1\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.73 (0.56 ; 0.93)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.018\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e0.84 (0.53\u0026nbsp;; 1.20)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e0.39\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e0.73 (0.54\u0026nbsp;; 0.97)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.035\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eModel 2\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e0.87 (0.65 ; 1.11)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.29\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e0.92 (0.58\u0026nbsp;; 1.33)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e0.69\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e0.81 (0.59\u0026nbsp;; 1.11)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0.20\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003epTau181\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eModel 1\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.38 (1.13 ; 1.70)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.002\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e0.98 (0.69\u0026nbsp;; 1.37)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e0.92\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e1.49 (1.14\u0026nbsp;; 1.98)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.004\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eModel 2\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.21 (0.98 ; 1.51)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e0.078\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e0.85 (0.57\u0026nbsp;; 1.24)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e0.41\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e1.41(1.07\u0026nbsp;; 1.88)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.017\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003et-Tau\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eModel 1\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.42 (1.16 ; 1.75)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.001\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e1.01 (0.71\u0026nbsp;; 1.41)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e0.95\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e1.53 (1.16\u0026nbsp;; 2.04)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.003\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eModel 2\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.24 (1.005 ; 1.56)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e0.048\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e0.89 (0.60\u0026nbsp;; 1.31)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e0.58\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e1.45 (1.10\u0026nbsp;; 1.96)\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e0.012\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003ctfoot\u003e\u003ctr\u003e\u003ctd colspan=\"8\"\u003eMultiple logistic regression based on the biomarker alone (model a), and after adjustment for age, modified Charlson score (excluding age), MMSE, and the presence of a caregiver at home (model b). Analysis on total population were further adjusted on sex\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd colspan=\"8\"\u003e\u003cem\u003eAll variables were standardized for analysis. Results can be interpreted as xx% change in risk of malnutrition associated with one standard deviation majoration in the biomarker of interest, after adjustment for confounding variable\u003c/em\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tfoot\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eIn women, malnutrition was positively associated with pTau181 (OR 1.41 [95% CI 1.07\u0026ndash;1.88], p\u0026thinsp;=\u0026thinsp;0.017) and T-tau (OR 1.45 [95% CI 1.10\u0026ndash;1.96], p\u0026thinsp;=\u0026thinsp;0.012), after adjustment for confounding factors. Despite an association of the Aβ42/Aβ40 ratio with malnutrition in our univariate analysis, this association was no longer significant after adjustment for confounding factors. No association between CSF biomarkers and malnutrition was found in men.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e Logistic regression models odds ratio adjusted for age, modified CCI score, MMSE score, and presence of a caregiver at home. Global population analysis is further adjusted on sex.\u003c/p\u003e\u003c/div\u003e"},{"header":"4. Discussion","content":"\u003cp\u003eIn this study, based on clinical practice, using the most recent diagnosis tools and nutritional status criteria, we described the specific prevalence of malnutrition in these populations. In the whole cohort, we found in both sexes that the fat mass index was positively correlated with amyloid ratio CSF levels and inversely correlated to pTau181 levels. In summary, a low fat mass index is associated with high amyloid and pTau181 brain accumulations. In AD patients, a lower fat mass index was inversely correlated with CSF T-tau levels, reflecting brain T-tau accumulation. In women, the muscle mass was inversely associated with pTau181 and t-tau. pTau181 and t-tau were independently associated with malnutrition after adjustment for confounding factors. Finally, regarding the other factors associated with malnutrition, we observed that age, sex, social isolation, and comorbidity burden were all associated with malnutrition in the univariate analysis.\u003c/p\u003e\u003cp\u003eWith regards to our first objective, the already reported prevalence of malnutrition in cognitively impaired individuals, as measured by the MNA questionnaire, varies widely from 2.9% to 17.4% according to previous studies [35\u0026ndash;37]. In our results, the prevalence of malnutrition and low muscle mass was much higher, including a significant sex-related disparity in disfavor of women. Indeed, we used the GLIM criteria, which include age-specific BMI thresholds, assessment of muscle mass and weight loss over an extended period. These criteria allow for a more specific and objective determination of the nutritional status, and could explain for the discordant findings between previous studies and our results. As illustrated by Ozer \u003cem\u003eet al.\u003c/em\u003e, in a population without cognitive decline, 32.2% of older adults without dementia were malnourished using the GLIM criteria, whereas the MNA and MNA-SF only identified malnutrition risk in 12.7% and 13.1% of subjects [38]. MNA primarily identifies the risk for malnutrition [17,21,24,35,37] without any confirmation and independently of age and sex; furthermore, the reliability of the questionnaire could be inferior in patients with cognitive decline, altogether potentially leading to an underestimated malnutrition. Overall, these results underscore the important need to implement rigorous nutritional assessment and management strategies, aiming at detecting, understanding, preventing, and treating malnutrition in this population.\u003c/p\u003e\u003cp\u003eOur second objective was to investigate the relationships between AD CSF biomarkers and body composition parameters. Previously, a cross-sectional study from Doorduijn et al. using the MNA score in a prospective cohort from daily practice of more than 500 AD patients and controls, reported an inverse association between both pTau181 and t-tau and fat-free mass, but no association with the Aβ42 peptide. However, analyses were not stratified by sex in this study, but were only adjusted for sex.[24]. In our study, we observed similar results, but only in women associated with an inverse association of pTau 181 and t-tau with muscle mass parameters. We mainly found an association of amyloid CSF biomarker (amyloid accumulation) and CSF pTau181 (pTau accumulation) with fat mass, which was not previously described in relation to CSF biomarkers, in mild cognitive impairment.\u003c/p\u003e\u003cp\u003eIn AD patients, a lower fat mass index was linked to total tau brain accumulation. These results imply that in neurocognitive disorders, low levels of body fat are associated with amyloid and pTau 181 brain accumulations and that in AD patients, a lower fat mass index was linked to increased neurodegeneration. Two studies in the general population have specifically examined the relationship between fat mass or muscle mass and plasma AD biomarkers and found different results. De Crom et al. showed that fat mass was positively associated with t-tau [39], while Hermesdorf \u003cem\u003eet al\u003c/em\u003e. found fat mass was inversely associated with β-amyloid 42 levels and positively associated with Aβ-40/Aβ-42 ratio [40]. These two studies focused on the general population without cognitive impairment, younger age, and higher BMI, which can explain, at least partially, the difference with our results. Moreover, unlike CSF biomarkers, the relationships between plasma biomarkers and body composition may be confounded by variations in volume of distribution, making it difficult to establish a true pathophysiological connection [41].\u003c/p\u003e\u003cp\u003eInterestingly, all the reported links between AD biomarkers and nutritional status suggest a possible common pathophysiological hypothesis. We found a marked association between amyloid, tau biomarkers and fat mass, both in men and women, suggesting that AD-related pathology and neurodegeneration may be linked to a preferential loss of fat mass relative to muscle mass. Leptin deficit may be a cause of this association [42]. Leptin is mainly produced by adipocytes and is involved in the regulation of appetite and energy expenditure; it also plays a neuroprotective role, stimulating neurogenesis and participating in neurocognitive functions such as memory or learning [43]. Ishii \u003cem\u003eet al.\u003c/em\u003e showed that AD mouse models exhibited reduced fat mass and lower leptin levels compared with wild-type controls. In response to these low leptin levels, AD mice revealed low levels of neuropeptide Y (NPY), an orexigenic peptide, and not high levels as expected [44]. In another AD mouse model, Robison \u003cem\u003eet al\u003c/em\u003e. suggested that impaired hypothalamic signaling could explain the inadequate response to low leptin levels observed in the context of AD, thus favoring weight and fat mass loss [45].\u003c/p\u003e\u003cp\u003eData from these preclinical models provide insights into potential mechanisms underlying our findings, highlighting the association of tau and amyloid CSF biomarkers with fat mass loss in both sexes. In AD patients, the links between neurodegeneration and low fat mass index could be associated with reduced leptin neuroprotection, as shown in our previous studies [42,46].\u003c/p\u003e\u003cp\u003eMoreover, our diagnostic subgroup results support a specific link between brain amyloid deposition and fat mass reduction, even in OND patients. Nevertheless, further studies investigating markers of neuroinflammation or neurodegeneration in relation to body composition changes across different neurodegenerative diseases are needed to better clarify the nature of this association.\u003c/p\u003e\u003cp\u003eAnother key finding of this study is the association between malnutrition and CSF AD biomarkers in women. The sex difference observed in our study is consistent with preclinical studies in this field. Lopez-Gamboro \u003cem\u003eet al.\u003c/em\u003e reported that neuroinflammation was associated with reduced food intake and subsequent weight loss in female mutant mice [47], in line with our results. However, in humans, other socio-environmental factors can be involved. Notably, women experienced more social isolation than men in our study, which could contribute to malnutrition.\u003c/p\u003e\u003cp\u003eThe strengths of our study include its multicenter design, involving both geriatric and neurological centers, allowing the inclusion of patients across a broad age range. We also specifically performed sex-stratified analyses, enabling sex specificities that could lead to adapted care according to sex. However, several limitations should be acknowledged. The absence of longitudinal data and missing information on prior weight loss likely led to an underestimation of the prevalence of malnutrition. Given the hypothesis of progressive, gradual weight loss due to reduced appetite in neurodegenerative diseases, cross-sectional measurements of fat and muscle mass do not capture individuals\u0026rsquo; changes in body composition during the disease. Moreover, despite exclusion criteria and adjustment for confounders, malnutrition is often multifactorial, and it remains challenging to account for all relevant factors, such as mood disorders, hospitalizations and environmental or caregiving conditions. Finally, limited statistical power may restrict the generalizability of our findings in men.\u003c/p\u003e"},{"header":"5. Conclusion","content":"\u003cp\u003eOur study highlights a close association between malnutrition, loss of fat and muscle mass, and AD CSF biomarkers, which could suggest a common pathophysiological link. Further longitudinal investigations, including the evaluation of appetite-regulating hormones (such as glucagon-like peptide-1 and others), are needed. In parallel, preventive strategies aimed at maintaining adequate nutritional status\u0026mdash;through balanced diets, early screening for malnutrition, and tailored nutritional interventions\u0026mdash;may help limit or delay cognitive decline and neurodegeneration potentially associated with malnutrition.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval\u003c/strong\u003e\u003cp\u003eAll participants participated in the BioCogBank protocol NCT06244875), and gave written consent for CSF and plasma collection for further analysis. They also provided consent for the use of their clinical data and the results of their CSF analyses. This study was approved by local and national Ethics Committees (\"Comit\u0026eacute; d'\u0026eacute;valuation et d'Ethique pour la recherche Paris Nord\" and by the \"Commission Nationale Informatique et Libert\u0026eacute;s\" (CNIL)).\u003c/p\u003e\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003cp\u003eNot applicable\u003c/p\u003e\u003c/p\u003e\u003cp\u003e\u003ch2\u003eCompeting interest\u003c/h2\u003e\u003cp\u003eNone\u003c/p\u003e\u003c/p\u003e\u003ch2\u003eFunding\u003c/h2\u003e\u003cp\u003eNone\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eKarl G\u0026ouml;tze: Conceptualization, Data curation, formal analysis, methodology, writing \u0026ndash; original draft, writing \u0026ndash; review and editingAgathe Vrillon: Data curation, writing \u0026ndash; original draft, writing \u0026ndash; review and editingManuel Sanchez\u0026nbsp;: Methodology, writing \u0026ndash; review and editingIn\u0026egrave;s Petit-Damico\u0026nbsp;: Data curation, writing \u0026ndash; original draft, writing \u0026ndash; review and editingElodie Bouaziz-Amar\u0026nbsp;: formal analysis, writing \u0026ndash; review and editingSophie Lacaille\u0026nbsp;:Data curation, writing \u0026ndash; review and editingClaire Hourr\u0026egrave;gue\u0026nbsp;: Data curation, writing \u0026ndash; review and editingEmmanuel Cognat\u0026nbsp;: Data curation, writing \u0026ndash; review and editingJacques Hugon\u0026nbsp;: Data curation, writing \u0026ndash; review and editingTh\u0026eacute;odore Decaix\u0026nbsp;: Data curation, writing \u0026ndash; review and editingJulien Dumurgier\u0026nbsp;: Data curation, writing \u0026ndash; review and editingAgathe Raynaud-Simon\u0026nbsp;: Methodology, writing \u0026ndash; review and editingMatthieu Lilamand\u0026nbsp;: Conceptualization, writing \u0026ndash; review and editingClaire Paquet\u0026nbsp;: Conceptualization, writing \u0026ndash; original draft, writing \u0026ndash; review and editing\u003c/p\u003e\u003ch2\u003eAcknowledgement\u003c/h2\u003e\u003cp\u003eNone\u003c/p\u003e\u003ch2\u003eData Availability\u003c/h2\u003e\u003cp\u003eThe dataset used and/or analysed during the current study are available from the corresponding author on reasonable request\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003e G S, M DR, A C, Em I, E M. 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J Psychiatr Res. 1975;12:189\u0026ndash;98. https://doi.org/10.1016/0022-3956(75)90026-6\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Guerin O, Soto ME, Brocker P, Robert PH, Benoit M, Vellas B, et al. Nutritional status assessment during Alzheimer\u0026rsquo;s disease: results after one year (the REAL French Study Group). J Nutr Health Aging. 2005;9:81\u0026ndash;4.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Soysal P, Dokuzlar O, Erken N, Dost G\u0026uuml;nay FS, Isik AT. The Relationship Between Dementia Subtypes and Nutritional Parameters in Older Adults. J Am Med Dir Assoc. 2020;21:1430\u0026ndash;5. https://doi.org/10.1016/j.jamda.2020.06.051\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Droogsma E, van Asselt DZB, Sch\u0026ouml;lzel-Dorenbos CJM, van Steijn JHM, van Walderveen PE, van der Hooft CS. 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Plasma Leptin Is Associated With Amyloid CSF Biomarkers and Alzheimer\u0026rsquo;s Disease Diagnosis in Cognitively Impaired Patients. J Gerontol A Biol Sci Med Sci. 2023;78:645\u0026ndash;52. https://doi.org/10.1093/gerona/glac234\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Garcia-Garcia I, Kamal F, Donica O, Dadar M, Alzheimer\u0026rsquo;s Disease Neuroimaging Initiative. Plasma levels of adipokines and insulin are associated with markers of brain atrophy and cognitive decline in the spectrum of Alzheimer\u0026rsquo;s Disease. Prog Neuropsychopharmacol Biol Psychiatry. 2024;134:111077. https://doi.org/10.1016/j.pnpbp.2024.111077\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Ishii M, Wang G, Racchumi G, Dyke JP, Iadecola C. Transgenic mice overexpressing amyloid precursor protein exhibit early metabolic deficits and a pathologically low leptin state associated with hypothalamic dysfunction in arcuate neuropeptide Y neurons. J Neurosci. 2014;34:9096\u0026ndash;106. https://doi.org/10.1523/JNEUROSCI.0872-14.2014\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Robison LS, Gannon OJ, Salinero AE, Abi-Ghanem C, Kelly RD, Riccio DA, et al. Sex differences in metabolic phenotype and hypothalamic inflammation in the 3xTg-AD mouse model of Alzheimer\u0026rsquo;s disease. Biol Sex Differ. 2023;14:51. https://doi.org/10.1186/s13293-023-00536-5\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e Bouaziz-Amar E, Neez J, Ibrahim F, Martinet M, Hourregue C, Dumurgier J, et al. Cerebrospinal fluid leptin in Alzheimer\u0026rsquo;s disease: relationship to plasma levels and to cerebrospinal amyloid. Clin Chem Lab Med. 2025; https://doi.org/10.1515/cclm-2025-0304\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003e L\u0026oacute;pez-Gambero AJ, Rosell-Valle C, Medina-Vera D, Navarro JA, Vargas A, Rivera P, et al. A Negative Energy Balance Is Associated with Metabolic Dysfunctions in the Hypothalamus of a Humanized Preclinical Model of Alzheimer\u0026rsquo;s Disease, the 5XFAD Mouse. Int J Mol Sci. 2021;22:5365. https://doi.org/10.3390/ijms22105365\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Table 1","content":"\u003cp\u003eTable 1 is not available with this version.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"alzheimers-research-and-therapy","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"azrt","sideBox":"Learn more about [Alzheimer's Research and Therapy](http://alzres.biomedcentral.com/)","snPcode":"13195","submissionUrl":"https://submission.nature.com/new-submission/13195/3","title":"Alzheimer's Research \u0026 Therapy","twitterHandle":"@AlzheimersRes","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Alzheimer’s disease, Neurofilament proteins, Cognitive domains performance, Neuroimaging, Neurodegenerative diseases, Cognitive decline","lastPublishedDoi":"10.21203/rs.3.rs-7980996/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7980996/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eINTRODUCTION\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAlzheimer's disease (AD) is associated with early weight loss, with unclear underlying mechanisms. Using real-world data, we aimed to investigate the links between biological AD profile, body composition, and nutritional status in patients with cognitive impairment.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMETHODS\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eCross-sectional study of patients with cognitive decline explored with cerebrospinal fluid (CSF) AD biomarkers and bioelectrical impedance analysis. Nutritional status was assessed using GLIM criteria. Analyses were stratified by sex and adjusted for age, MMSE, comorbidities, and the presence of a caregiver at home.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eRESULTS\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAmong 420 patients, the fat mass index was positively associated with the Aβ42/40 ratio and negatively with pTau181 levels (p \u0026lt; 0.05). Malnutrition was more frequent in women than men (p = 0.0059). In women, malnutrition was associated with higher t-Tau and pTau181 levels (p \u0026lt; 0.02). In both sexes, pTau181 levels were inversely associated with muscle mass (p = 0.041).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCONCLUSION\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThese results suggest possible pathophysiological links between AD CSF biomarkers and nutritional status.\u003c/p\u003e","manuscriptTitle":"Negative association of fat mass index and Alzheimer’s disease cerebrospinal fluid biomarkers in cognitively impaired patients","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-13 07:53:51","doi":"10.21203/rs.3.rs-7980996/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-11-17T21:43:20+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-17T21:21:19+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-15T21:21:11+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-13T13:14:35+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"249871945952006198600719813293749405320","date":"2025-11-05T16:08:23+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-05T10:58:19+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-03T15:20:46+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"324561521392078481043703959456565186390","date":"2025-11-03T13:24:46+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"2223227850816988681081889016606806635","date":"2025-11-03T08:30:20+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"255785990465351167000874458998609241067","date":"2025-10-31T19:18:35+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"153822217657127786913312376814103309779","date":"2025-10-31T15:53:51+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-10-31T15:50:02+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-10-31T05:48:49+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-10-31T05:48:25+00:00","index":"","fulltext":""},{"type":"submitted","content":"Alzheimer's Research \u0026 Therapy","date":"2025-10-29T14:28:34+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"alzheimers-research-and-therapy","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"azrt","sideBox":"Learn more about [Alzheimer's Research and Therapy](http://alzres.biomedcentral.com/)","snPcode":"13195","submissionUrl":"https://submission.nature.com/new-submission/13195/3","title":"Alzheimer's Research \u0026 Therapy","twitterHandle":"@AlzheimersRes","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"98ecdc8c-da33-40ab-b9d6-67932da171e6","owner":[],"postedDate":"November 13th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2026-01-26T16:02:12+00:00","versionOfRecord":{"articleIdentity":"rs-7980996","link":"https://doi.org/10.1186/s13195-026-01965-1","journal":{"identity":"alzheimers-research-and-therapy","isVorOnly":false,"title":"Alzheimer's Research \u0026 Therapy"},"publishedOn":"2026-01-22 15:56:56","publishedOnDateReadable":"January 22nd, 2026"},"versionCreatedAt":"2025-11-13 07:53:51","video":"","vorDoi":"10.1186/s13195-026-01965-1","vorDoiUrl":"https://doi.org/10.1186/s13195-026-01965-1","workflowStages":[]},"version":"v1","identity":"rs-7980996","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7980996","identity":"rs-7980996","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}