Isolation and characterization of annonacin from Annona muricata L using semipreparative HPLC

Isolation and characterization of annonacin from Annona muricata L using semipreparative HPLC | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Isolation and characterization of annonacin from Annona muricata L using semipreparative HPLC Antônio Adailson de Sousa Siva, Rosa Helena Andrade Honorio Lima, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8604748/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The secondary metabolism of the species Annona muricata , popularly known as soursop (Annonaceae), produces a class of substances known as acetogenins. Given the significant pharmacological importance of these secondary metabolites, this work aims to apply chromatographic techniques to isolate and confirm the identity of the main acetogenin in Annona muricata L., highlighting the importance of this approach for large-scale production of the active principles of this compound. The ethanolic extract of A. muricata seeds was fractionated by classical column chromatography, and the resulting fraction was analyzed by TLC, yielding a fraction rich in acetogenins. From this fraction, 600 mg was isolated by semi-preparative reverse-phase liquid chromatography, yielding 150.52 mg of isolated acetogenin. After isolation, the sample was again analyzed by chromatography in analytical mode to confirm its purity and subjected to NMR for identity confirmation, which revealed it to be annonacin, the major acetogenin of Annona muricata L. in both leaves and seeds, corroborating literature data. Thus, this study contributed to the valorization of this species through spectroscopic and chromatographic analysis of its chemical constituents, as well as to the work of natural products chemistry in the prospecting and isolation of these constituents. Annonacin Annona muricata Nuclear magnetic resonance High-performance liquid chromatography Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8604748","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":585687037,"identity":"53502a4d-9fb1-4f0c-854a-36e1eb559d24","order_by":0,"name":"Antônio Adailson de Sousa Siva","email":"","orcid":"","institution":"Federal University of Ceará","correspondingAuthor":false,"prefix":"","firstName":"Antônio","middleName":"Adailson de Sousa","lastName":"Siva","suffix":""},{"id":585687038,"identity":"c391868f-f36d-46d8-aa19-6945d841bca2","order_by":1,"name":"Rosa Helena Andrade Honorio Lima","email":"","orcid":"","institution":"Federal 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