Green Biogenic Synthesis of Sn-ZrO2 Nanocomposites Using Musa paradisiaca Peel Extract Under Sonication for Biological and Adsorption Efficacy

Green Biogenic Synthesis of Sn-ZrO2 Nanocomposites Using Musa paradisiaca Peel Extract Under Sonication for Biological and Adsorption Efficacy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Green Biogenic Synthesis of Sn-ZrO2 Nanocomposites Using Musa paradisiaca Peel Extract Under Sonication for Biological and Adsorption Efficacy Diksha Bhardwaj, shobhana sharma This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4946370/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The production of nanoparticles using plant extracts has been the subject of much exploration and study in recent times since it is a cost-effective and environmentally friendly method that reduces the use of hazardous chemicals. In this work, Musa paradisiaca (banana) peel extract was used to synthesize Sn-ZrO 2 nanocomposites under ultrasonic irradiation. As a capping and reducing agent in the manufacture of Sn-ZrO 2 nanocomposites, banana peel extract is crucial. Sn-ZrO 2 nanocomposites were synthesized in a green manner were effectively evaluated using a FT-IR spectroscopy, UV-Vis spectrophotometer, X-ray diffraction analysis (XRD), energy-dispersive X-ray spectroscopy and scanning electron microscopy (SEM-EDS). Studies have been conducted on the antimicrobial properties of synthesized ZrO 2 nanocomposites doped with tin against both Gram positive and Gram negative pathogenic bacteria and fungus. Furthermore, free radical scavenging activity against the DPPH and ABTS assay was used to assess the antioxidant activity of green Sn-ZrO 2 nanocomposites. The biomimetic synthesised Sn-ZrO 2 nanocomposites demonstrated robust antioxidant activity and significant antimicrobial activity that was on par with standard. Further, Sn-ZrO 2 nanocomposites shows excellent adsorption capacity of malachite green dye. Musa paradisiaca antimicrobial adsorption capacity antioxidant Sn-ZrO2 nanocomposites Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Introduction Nanostructures are essential research tools in various fields, and nanotechnology is a rapidly expanding discipline with diverse applications in science and technology (Haleem et al. 2023). A great deal of research has been conducted on metal and metal oxide nanoparticles because of their exceptional electrical, optical, magnetic, catalytic, and medicinal properties (Bukhari et al. 2021). Conventional techniques for creating metal and metal oxide nanoparticles use stabilizing and reducing chemical agents, which can be costly and harmful to the environment (Nair et al. 2022). As a result, scientists are currently searching for different green synthesis techniques which minimize the hazardous substances used in the nanoparticles-producing process (Ying et al. 2022). In recent years, there has been extensive research on the eco-friendly, minimal-use method of green synthesis of metals and metal oxides nanoparticles employing plant extracts (Shafey et al. 2020). Zirconium oxide (ZrO 2 ) nanoparticles are a highly valuable material in various fields, particularly in the dentistry (Kumari et al. 2022). Their unique surface chemistry, high chemical and thermal stability, cost-effectiveness, non-hazardous and sustainable nature, clean photocatalytic properties, and impressive morphologies have significantly impacted both academia and industry (Hassaan et al. 2023). ZrO 2 is a valuable material for water pollutant removal due to its high electron mobility, direct band gap, anisotropic growth, chemical stability, high photocatalytic efficiency, and ease of morphological control. Zirconium oxide is environmentally friendly and possesses outstanding biocompatibility (Bannunah 2023). The usefulness of zirconia is limited by two intrinsic properties: a high band gap and quick electron-hole pair recombination (Khattab et al. 2021). The synthesis of metal nanoparticles (Ag, Au, Sn, Cu, Ru, Pd, etc.) doped on metallic oxide surfaces has recently attracted a lot of interest in the fields of material science and nanotechnology due to its significantly enhanced applications in a variety of fields (Szczyglewska et al. 2023). Furthermore, studies show that tin-doping controls the cytotoxicity of zirconia nanoparticles by killing human cancer cells alone while leaving healthy cells unharmed (Barbasz et al. 2024). It was recently discovered that Sn-ZrO 2 generate a reactive oxygen species (ROS) that can destroy microbial colonies in the absence of light irradiation (Mammari et al. 2022). Sn-ZrO 2 nanocomposites formation is a good choice because of their unique optical-physiological and biological properties, ease of synthesis at a low cost, high availability, and low toxicity. Sn-ZrO 2 nanocomposites have been synthesized using a variety of methods, including the thermal decomposition, sol-gel process, co-precipitation and electrochemical oxidation process (Narayanan et al.2019; Bharti and Sadhu 2022). Although, some of these physio-chemical methods not only violate environmental regulations but also cause poor dispersibility and irreversible nanoparticle agglomeration, which reduces biological activity (Joudeh and Linke 2022). Consequently, the synthesis of different nanomaterials utilizing plant extracts has recently caught the interest of researchers as a means of getting around these limitations (Ying et al. 2022). However, less research has been done on the synthesis of Sn-ZrO 2 nanocomposites utilizing plant extracts. The synthesis of nanocomposites typically requires two or more phases and a lengthy reaction procedure, although plant mediated synthesis support green approaches (Ying et al.2022). The ultrasonic energy used as a non-conventional source act as a workable and environmentally beneficial alternative for material production (Shen et al. 2023). Some research indicates that the cavitational action of ultrasonic waves during nanomaterial manufacturing reduced the reaction time and produced smaller particles (Sandhya et al. 2021). During cavitation, bubbles form and burst, rapidly producing large quantities of non-aggregated nanoparticles (Khairani et al. 2023). In light of the many uses and paucity of research on plant-mediated nanocomposites synthesis and as part of our continuing endeavour on the designing and green synthesis of functional nanocomposites (Bhardwaj et al. 2021; Bhardwaj et al. 2019), we have shown here for the first time a green banana peel extract assisted synthesis of Sn-ZrO 2 nanocomposites under sonication. Biomolecules included in banana peel extract serve as stabilizing and reducing agents in the synthesis of nanomaterials (Ohiduzzaman et al. 2024; Bao et al. 2021). Materials and Methods Bananas were collected from the local area of Jaipur district, Rajasthan, India. The chemicals used in this study were zirconyl nitrate [ZrO(NO 3 ) 2 .xH 2 O], stannous chloride (SnCl 4 ), ethanol, ZrO 2 , 2, 2-Diphenyl1-picrylhydrazyl (DPPH), ABTS (2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)), etc. were purchased from Merck Chemical Company (Darmstadt, Germany) and used as such without further purification. Characterization methods and Instruments UV-Vis, FTIR, XRD, and SEM-EDS studies were used to characterize Sn-ZrO 2 nanocomposites that were green-synthesised. Using a quartz cuvette filled with de-ionized water as a reference, UV-Vis NIR spectrophotometer was employed. FTIR spectra were captured in a spectral range of 4000-400 cm -1 using KBr pellets. The X-ray diffractometer, which was outfitted with a CuKα radiation (λ=1.54060 Å) was used to perform XRD measurements. Surface morphology and form of the nanocomposites were evaluated using FE-SEM. The FE-SEM and EDS detector are connected so that elements included in nanoparticles can be identified and their chemical composition can be examined. The ultrasound assisted reactions were performed using an ultrasonic processor probe system. For ten minutes, the operating conditions involved a 30-second on and 30-second off pulse with 50% amplitude. Preparation of Musa paradisiaca (Banana) peel extract After gathering the Musa paradisiaca peels, they were carefully cleaned under running water to get rid of any associated dust particles, and then dried. About 20 gm of dried banana peel were powdered, and 100 ml of deionized water was added. In order to prepare the peel extract, the mixture was heated at 80°C for ten minutes. After allowing the combination to cool to room temperature, Whatman No. 1 filter paper was used to filter the mixture. After the residue was eliminated, the filtrate was utilized to create nanocomposites. Synthesis of Sn-ZrO 2 nanocomposites using Musa paradisiaca peel extract To create Sn-ZrO 2 nanocomposites in an environmentally friendly manner, a cylindrical glass vessel containing a precursor solution of 0.1M zirconyl nitrate and 15 ml deionized water was subjected to a 10-minute sonication period. Subsequently, a 1 mM stannous chloride solution was applied dropwise while being continuously sonicated to a 25 ml banana peel extract. The solution turned gray after ten minutes as a result of surface plasmon resonance (SPR) activation, which showed that Sn-ZrO 2 nanocomposites were forming. The precipitation of Sn-ZrO₂ nanocomposites was completed by centrifuging the solution for 20 minutes at 6000 rpm. After three water washes to eliminate byproducts, the precipitate was dried in an oven at 80 °C for an entire night, and it was then annealed for two hours at 500 °C. Antimicrobial activities The traditional well-diffusion method was employed to examine the antimicrobial potential of produced Sn-ZrO 2 nanocomposites, as previously described (Sharma et al. 2018; Sharma et al. 2019; Sharma et al. 2022). Two distinct fungi, C. albicans and C. tropicalis , were evaluated for their antifungal activity, while pathogenic microorganisms classified as Gram-negative ( K. pneumonia & P. aeruginosa ) and Gram-positive ( B. subtilis & S. aureus ) were tested for their antibacterial activity. Every strain was evenly swabbed onto the sterilized nutritional agar petriplates using cotton swabs. Then, using a sterile plastic rod, 8 mm diameter wells were pierced into the inoculation plates. Four concentrations (20, 40, 60, and 80 µg/ml) of produced Sn-ZrO 2 nanocomposites were added, accordingly, to the specified wells using a micropipette. After incubating for 24 hours at 37°C, the antimicrobial activity was assessed by measuring the diameter of the inhibitory zone in millimeters (mm) using a standard scale. The antimicrobial activity of the resulting Sn-ZrO 2 nanocomposites was compared with that of ZrO 2 nanoparticles in order to measure the effectiveness of Sn loading in nanocomposites and also compared with reference standard. Antioxidant activity The antioxidant potential of the green produced ZrO 2 nanoparticles and Sn-ZrO 2 nanocomposites was evaluated and compared with standard using DPPH and ABTS tests. DPPH assay According to a previous approach (Verma et al. 2022), the antioxidant activity of green synthesized Sn-ZrO 2 nanocomposites and ZrO 2 nanoparticles was examined using DPPH assay. One milliliter of DPPH solution (0.1 millimeter of DPPH in methanol) was filled with different concentrations of synthesized Sn-ZrO 2 nanocomposites and ZrO 2 nanoparticles, and the test tubes were labeled appropriately. After shaking the reaction mixture, it was left at room temperature for 30 minutes in a dark environment. At 517 nm, the absorbance was measured spectrophotometrically. ABTS assay The ABTS free radical scavenging activity of ZrO 2 nanoparticles and green synthesised Sn-ZrO 2 nanocomposites was examined using the previously reported methodology, with some minor adjustments (Haq et al. 2021). By reacting 2.45mM of potassium persulfate (K 2 S 2 O 8 ) with 7.mM of the ABTS stock solution, ABTS radical cation (ABTS +. ) stock solution was prepared. The absorbance at 734 nm was measured following an overnight incubation period at room temperature. ZrO 2 nanoparticles and synthesized Sn-ZrO 2 nanocomposites were added individually with ABTS +. at varying concentrations, and they were once more incubated for 15 minutes in a dark environment. As a control solution, ABTS reagent was employed without sample. Batch Adsorption Studies: Malachite green (MG) dye was made as a stock solution (100 ppm) in deionized water, and subsequently diluted to yield a range of samples from 1 to 12 ppm. The effects of various parameters on the removal of MG dye using Sn-ZrO 2 nanocomposites during the batch adsorption method are investigated. These parameters include adsorbent dosage (5, 10, 15, 20, 25, 30, 35 and 40 mg), contact time (5, 10, 15, 20, 25, 30 min), pH (2 to 12), and temperature (303, 323 and 353 K). The adsorption capacity of MG dye was determined using Equation 1, while the removal efficiency (%) was calculated with Equation 2. (q e ) = (C o -C e ) V/M Equation (1) Removal (%) = (C o -C e ) / C o × 100 Equation (2) where M (mg) is the mass of the Sn-ZrO 2 nanocomposites, V (L) is the volume of MG dye obtained in an aqueous solution, and C o (mg/L) and C e (mg/L) are the initial and equilibrium concentrations, respectively, of MG dye. Results and discussion UV–Vis Spectrophotometer: Fig. 1, comprises the room-temperature optical absorption of Sn-ZrO 2 nanocomposites in the range of 200–800 nm. The sharp absorption peak seen at around 260 nm can be attributed to ZrO 2. (Chelliah et al. 2023). The synthesized nanocomposites demonstrated absorbance band from 280-550 nm shows deposition of Sn nanoparticles on the surface of nanocomposites successfully (Sohail et al. 2020). FTIR analysis : Fig. 2 reveals the FTIR spectrum of green synthesis Sn-ZrO 2 nanocomposites. The characteristic broad peaks of OH groups are found at 3428.71 and 1628.34 cm -1 corresponds to the water molecules that have been absorbed on the surface of the nanoparticles. The stretching and bending vibrational modes of the water molecule are responsible for these broad peaks (Sagadevan et al. 2016). The weak bands at 2922.36, 2852.34, and 1384.31 cm −1 were associated with the vibrations of organic residuals (Alharthi et al. 2020). The absorption band that was most prominent and sharpest emerged at 1121.69 and 617.74, cm −1 potentially indicating a correlation with the Zr–O bonds. The bending vibration of hydroxyl groups attached to ZrO 2 is responsible for the peaks at 1121.69 cm−1 and 617.74 cm −1 . The Zr–O vibration is represented by the band at 617.74 cm −1 , and the breadth of the band indicates that the ZrO 2 powders are nano crystals (Yakout et al. 2014). XRD: X-ray diffraction measurements were used to investigate the crystallinity and phase form of the synthesised nanomaterials. Fig. 3 demonstrate the XRD pattern of Sn-ZrO 2 nanocomposites and bare ZrO 2 nanoparticles. The diffraction peaks in a wide range angle of 2θ are at 30.64°, 35.22°, 50.26°, and 60.17° corresponds to the crystal planes of (101), (110), (112), and (211), respectively, attributed to the preparation of monoclinic phase of ZrO 2 nanoparticles (Horti et al. 2020) (JCPDS card no. 01‐075‐2550). Comparison of XRD pattern reveals the appearance of strong intense peaks at 2θ = 30.10° and 35.05° to be (101) and (110) crystal planes of Sn doping on ZrO 2 as shown in Fig. 3 (Długosz et al. 2021). The XRD patterns do not exhibit distinctive peaks associated with Sn, indicating that all Sn atoms have successfully integrated into the ZrO 2 lattice. Additionally, the absence of any other diffraction peaks confirms the synthesized nanocomposites high purity. SEM and EDS analyses The detailed morphology, particle size, and shape of the biomorphic Sn-ZrO₂ nanocomposites were examined using SEM analysis. The results revealed a non-uniform distribution of spherical tin particles on the surface of zirconia nanoparicles. The average size of Sn-ZrO 2 nanocomposites is 25nm-50nm (Fig. 4a, b). The elemental composition of the Sn-ZrO₂ nanocomposites was determined by EDS analysis, confirming the presence of zirconium (Zr), tin (Sn), and oxygen (O) as shown in Fig. 5. Additional presence of elements (calcium) is also observed due to the presence of some phytochemicals from extract. Adsorption performance of Sn-ZrO 2 nanocomposites: This experimental work was conducted using a 10 ppm MG dye solution. 30 mg of Sn-ZrO 2 nanocomposites were put to a beaker containing 20ml of MG dye solution. The mixture is shaken constantly for 25 minutes or until equilibrium is reached. The Sn-ZrO 2 nanocomposites was used to study the adsorption of MG dye from an aqueous solution at different dye concentrations, contact times, dosages of the adsorbent, pH levels, and temperatures. The absorbent was changed from 5 to 40 mg/L while all other parameters remained same. The pH was adjusted to a range between 3 and 10 ppm using 0.1 M HCl and 0.1 M NaOH solutions. The shake took longer than five minutes to complete. The adsorbate solution was taken out and filtered once the allotted time had elapsed in order to isolate the adsorbent. Following filtering, a UV-Vis spectrophotometer that had previously been calibrated was used to measure the solution's concentration at 615 nm. Alterations in adsorbent dose: Doses are crucial during the adsorption process. To investigate the effect of the Sn-ZrO 2 nanocomposites dosage on the adsorption of MG dye (10 mg/L), adsorbent dosages ranging from 5 mg to 40 mg were used. The removal effectiveness was increased from 5 to 40 mg of Sn-ZrO 2 nanocomposites, as shown in Fig. 6(a). This is because larger surface areas and more active sites are present in adsorbent. It was shown that the Sn-ZrO 2 nanocomposites had an optimal dose of 30 mg. Once the optimal adsorbent dose has been reached, the removal efficiency falls. Variation with pH: Since pH influences the surface charge of the adsorbate (MG dye) and Sn-ZrO 2 nanocomposites adsorbent, it plays a significant part in adsorption processes. The influence of pH on the effectiveness of nanocomposites' removal of MG dye is shown in Fig. 6b. To adjust the required pH, 0.1 M sodium hydroxide or 0.1 M hydrochloric acid was used. As the pH rose from 8 to 10, the removal efficiency significantly declined after increasing from 3 to 8. Effect of temperature : Temperature is thought to be a significant element that affects how well the MG is adsorbed and removed. At various temperatures between 303 and 353 K, the adsorbent dosage (30 mg/L), pH (8), contact period is 25 min, and constant agitation speed (450 rpm) were maintained in order to assess the impact of temperature on MG dye adsortion. As the temperature was elevated from 303 to 353 K, the rate of MG adsorption on Sn-ZrO 2 nanocomposites decreased, as shown in Fig. 7(a). This suggests that the adsorption process was somewhat endothermic. Influence of contact time: Analyzing the effect of the time of contact is important because the results of this type of study provide basic information about the rate at which the adsorption process reaches equilibrium. The effect of altering the contact time within the range of 15 to 20 minutes on the adsorption capacity was examined, keeping all other parameters fixed. The experiment started with fast dye adsorption, which gradually slowed down when the equilibrium condition was approached after around 15-20 minutes, based on the results displayed in Fig 7(b). Fig. 8 outlines a proposed mechanism for the formation of Sn-ZrO 2 nanocomposites. Amorphous hydrous oxide precipitates are formed when zirconium nitrate is hydrolyzed in aqueous circumstances, resulting in Zr(OH) 4 , which is naturally unstable and prone to condensation processes (Muthulakshmi et al. 2023; Chelliah et al. 2023). These condensation reactions are catalyzed by the hydroxyl groups present in banana peel extract, which are renowned for their antioxidant properties. Meanwhile, in the aqueous solution, tin chloride quickly separates into tin and chloride ions. As seen in the Fig. 8, the reducing phytochemicals in the extract bind and cap the tin ions to produce stable nanoparticles. The primary organic substances accountable for this process include the carotenoids and other phytonutrients found in banana peel extract, as well as anthocyanins, delphinidin, cyaniding, and catecholamines (Hikal et al 2022). The resultant Sn-ZrO 2 nanocomposites are then subjected to a 500°C calcination process in order to remove remaining phytochemical residue and water molecules. Antimicrobial activities of synthesised Sn-ZrO 2 nanocomposites Green synthesized Sn-ZrO 2 nanocomposites and ZrO 2 nanoparticles were tested against Gram positive and Gram negative bacteria, including B. subtilis , S. aureus , K. pneumonia and P. aeruginosa . Fig. 9 illustrates the zone of inhibition observed in bacteria due to the synthesized Sn-ZrO₂ nanocomposites at four different concentrations, compared to ZrO₂ nanoparticles. At an 80 µg/mL concentration of Sn-ZrO₂ nanocomposites, the largest zone of inhibition was recorded for K. pneumoniae (21 mm), followed by B. subtilis (20 mm). The smallest inhibitory zone was observed for S. aureus (5 mm) at a 20 µg/mL concentration of Sn-ZrO₂ nanocomposites. Through conducting studies with varying concentrations of nanocomposites, we discovered that the zone of inhibition rises as the concentration of Sn-ZrO 2 nanocomposites increases (Table 1). Bacterial membrane integrity is further compromised by lipid peroxidation, which is influenced by elevated levels of ROS. As the concentration of nanoparticles rises, the breakdown of the bacterial cell wall causes the bacteria to die (Ozdal et al. 2022). The likelihood that the nanoparticles will penetrate and harm the bacterial membrane increases with their size (Ozdal et al. 2022). The passage of nanoparticles across the plasma membrane has been facilitated by the presence of ion channels and transporter protein (Chen et al. 2020). The increase level of ROS affects lipid peroxidation in bacteria which further influence the integrity of bacterial membrane. The destruction of the bacterial cell wall results bacterial death with increase in concentration of nanoparticles (Juan et al. 2021). In comparison to ZrO 2 nanoparticles, the Sn-ZrO 2 nanocomposites exhibits a larger zone of inhibition. When tin ions are released from a Sn-ZrO 2 nanocomposites, it strengthens its ability to connect with bacterial enzymes that inactivate bio cells by breaking through their cell walls and damaging the bacteria. (Nikolova et al. 2020). Table 1: Zone of inhibition (mm) at various concentrations of green-synthesised Sn-ZrO 2 nanocomposites and ZrO 2 nanoparticles. Zone of Inhibition in mm Gram negative bacteria Gram positive bacteria Concentration K. pneumoniae P. aeruginosa B. subtilis S. aureus ZrO 2 Sn-ZrO 2 ZrO 2 Sn-ZrO 2 ZrO 2 Sn-ZrO 2 ZrO 2 Sn-ZrO 2 20µg/ml 8 12 5 8 7 9 4 5 40 µg/ml 10 13 7 10 12 14 6 8 60 µg/ml 14 17 10 15 16 18 9 12 80 µg/ml 16 21 11 18 18 20 10 13 Similarly, ZrO 2 nanoparticles and green-synthesised Sn-ZrO 2 nanocomposites were tested for their antifungal properties against two different fungi, C. albicans and C. tropicalis , as shown in Fig. 10. The synthesized Sn-ZrO 2 nanocomposites had strong antifungal activity against C. albicans , but only weak activity against C. tropicalis . As shown in Table 2, a relatively small amount of green Sn-ZrO₂ nanocomposites (20 µg/mL) was sufficient to disrupt the fungal cell membrane and ultimately kill the fungi. Comparing the antifungal activity of the synthesized Sn-ZrO 2 nanocomposites to ZrO 2 nanoparticles, it demonstrates a greater zone of inhibition of Sn-ZrO 2 nanocomposites much like the antibacterial assay. The higher antifungal activity of synthesised Sn-ZrO 2 nanocomposites was partly attributed to the smaller particle size attained through sonication. This is because smaller particles have a larger surface to volume ratio, which permits more drug molecules to be adsorbed on the surface. These molecules are anticipated to act as a powerful agent in breaking down cell walls (Bruna et al. 2021). The findings show that, in comparison to ZrO 2 nanoparticles, synthesized Sn-ZrO 2 nanocomposites is a more effective antimicrobial agent with a greater potential to kill germs. Table 2: Zone of inhibition (mm) at different concentrations of green synthesised Sn-ZrO 2 nanocomposites and ZrO 2 nanoparticles against C. albicans and C. tropicalis Concentration Zone of Inhibition in mm C. albicans C. tropicalis ZrO 2 Sn-ZrO 2 ZrO 2 Sn-ZrO 2 20µg/ml 4 8 2 4 40 µg/ml 6 9 4.2 6.5 60 µg/ml 9.2 11 6 8.3 80 µg/ml 11.5 13 9 10.4 Nonetheless, Musa paradisiaca has long been a well-known traditional herb used in medicinal field. Its exceptional biological potentials are an added benefit, and combined with the potent biological properties of tin and ZrO 2 , these properties would greatly encourage the green synthesis of Sn-ZrO 2 nanocomposites utilizing this well-known herb. Previous research has also shown that biosynthesised nanoparticles have potent antimicrobial efficacy than pure chemically synthesized nanoparticles. (Khan et al. 2020). Antioxidant activity of Sn-ZrO 2 nanocomposites Free radicals are neutralized by an antioxidant substance, which halts the oxidation process. Green synthesised Sn-ZrO 2 nanocomposites and ZrO 2 nanoparticles were tested against DPPH at various doses in an antioxidant assay (Fig. 11a). Significantly, Sn-ZrO 2 nanocomposites showed higher radical scavenging activity than ZrO 2 nanoparticles, indicating that the inclusion of tin particles improves the antioxidant nature of nanocomposites by effectively separating electron-hole pairs (Tran et al. 2022). The findings demonstrated that the Sn-ZrO 2 nanocomposites inhibits DPPH activity in a dose-dependent manner, means that radical scavenging assay enhances with the increase in concentration of the nanocomposites. The antioxidant experiment was conducted against ABTS using varying quantities of ZrO 2 nanoparticles and green synthesised Sn-ZrO 2 nanocomposites (Fig. 11b). Peels of Musa paradisiaca are rich in bioactive substances (polyphenols and oils containing essential fatty acids) that may be able to be scavenged because of their hydroxyl groups (Widoyanti et al. 2023). Similar to DPPH scavenging, dose-dependent action for ABTS was also observed. At greater concentrations, the green-synthesised Sn-ZrO 2 nanocomposites demonstrated stronger activity in blocking the ABTS radical than ZrO 2 nanoparticles. Musa paradisiaca peel extract contains phytochemicals, which are well-known for their antioxidant qualities. Tin and ZrO 2 's inherent antioxidant properties also contributes to the extract's increased scavenging activity, making it more powerful and active. Based on the aforementioned findings, it can be concluded that the environmentally friendly green Sn-ZrO₂ nanocomposites, synthesized using Musa paradisiaca peel extract under ultrasound irradiation, is a more viable option for antioxidant drugs compared to ZrO₂ nanoparticles and serves as a superior alternative to chemically synthesized options. Plausible mechanism responsible for the different applied dimensions of Sn-ZrO 2 nanocomposites Fig. 12 illustrates a plausible mechanism for the application of Sn-ZrO₂ nanocomposites in the adsorption potential, antimicrobial activity and antioxidant activity. Through metabolic processes, biogenic Sn-ZrO 2 nanocomposites most likely interacts with the membranes and cell walls of bacteria. They are more likely to produce ROS, which can lead to a variety of problems, including the deactivation of vital enzyme and protein functions. Consequences include rupture of the cell membrane, cytoplasmic leakage, damage to proteins, DNA, and mitochondria, and finally, cell death (Kumar et al. 2020). The Sn-ZrO 2 nanocomposites, which was created from banana peel extract, demonstrated superior efficacy in scavenging a range of ROS when tested for antioxidant activity using the ABTS and DPPH free radical assay (Kumar et al. 2020). The adsorption mechanism of MG dye on nanocomposites surfaces is influenced by various interactions, which are affected by specific phytochemicals. These include π–π stacking interactions between the surface of Sn-ZrO 2 nanocomposites and aromatic rings of MB, hydrogen bonding interactions between the functional groups on the nanocomposites surface and the nitrogen atoms of MG dye, and electrostatic interactions between the positively charged nitrogen atoms present on the dye and the negatively charged functional groups on the nanocomposites surface (Trieu et al. 2023). Conclusion This work presents the synthesis of Sn-ZrO 2 nanocomposites by means of a straightforward, economical, and environmentally sustainable method that employs Musa paradisiaca peel extract as a bio-reductant under ultrasonic irradiation. The reduction and stability of the nanocomposites were accomplished using the aqueous peel extract that contained phytoconstituents. The newly synthesized Sn-ZrO 2 nanocomposites was characterized by using FTIR, UV-Vis, XRD, and SEM-EDS techniques. The Sn-ZrO 2 nanocomposites was tested for antimicrobial and antioxidant properties, and dose-dependent variation was observed. When compared to ZrO 2 nanoparticles, the green-synthesised Sn-ZrO 2 nanocomposites shown superior biological activities that were comparable to benchmark. It is observed that Sn-ZrO 2 nanocomposites possess excellent adsorption capacity than ZrO 2 for MG dye. The current sonochemical synthesis process has many benefits, including low temperature, fast reaction times, and good surface dispersibility of the doped metal, which results in smaller particles with higher yields. Declarations Acknowledgements The authors are highly thankful to MRC, MNIT Jaipur for providing spectroscopic facilities, and Applied Seminal Jaipur for doing antimicrobial and antioxidative studies of the samples. Authors' contributions S.S. - conceptualization, methodology, formal analysis, investigations, resources, supervision, writing original draft, visualization; D.B.— conceptualization, methodology, formal analysis, investigations, resources, data curation, supervision, visualization, writing — review and editing. All authors contributed to the manuscript and approved the final version for publication. Funding None Data Availability All supporting data are available in the article. Ethics approval Not applicable. Consent to participate Not applicable. Consent for publication All authors approved the final manuscript. Competing interests The authors declare no competing interests. References Alharthi F A, Alghamdi A A, Al-Zaqri N, H S. Alanazi, A A Alsyahi, A E Marghany, N Ahmad (2020) Facile one-pot green synthesis of Ag–ZnO Nanocomposites using potato peeland their Ag concentration dependent photocatalytic properties. Sci Rep 10: 20229. https://doi.org/10.1038/s41598-020-77426-y Bannunah A M (2023) Biomedical Applications of Zirconia-Based Nanomaterials: Challenges and Future Perspectives. Molecules, 28(14): 5428. https://doi.org/10.3390/molecules28145428 Bao Y, He J, Song K, Guo J, Zhou X, Liu S (2021) Plant-Extract-Mediated Synthesis of Metal Nanoparticles. J. Chem., 2021:| 6562687. https://doi.org/10.1155/2021/6562687 Barbasz A M, Dyba B (2024) Direct Interaction of Zirconia Nanoparticles with Human Immune Cells. Biophysica, 4(1): 83-91; https://doi.org/10.3390/biophysica4010006 Bhardwaj D, Singh A, Singh R (2019). Eco-compatible sonochemical synthesis of 8-aryl-7,8-dihydro-[1,3]-dioxolo[4,5-g]quinolin-6(5H)-ones using green TiO2. Heliyon. 5 (2), e01256. https://doi.org/10.1016/j.heliyon.2019.e01256. Bhardwaj D, Singh R (2021) Green biomimetic synthesis of Ag–TiO 2 nanocomposite using Origanum majorana leaf extract under sonication and their biological activities. Bioresour. Bioprocess. 8: 1. https://doi.org/10.1186/s40643-020-00357-z Bharti K, Sadhu K K (2022) Syntheses of metal oxide-gold nanocomposites for biological applications. Res. Chem. 4:100288.https://doi.org/10.1016/j.rechem.2022.100288 Bruna T, Maldonado-Bravo F, Jara P, Caro N (2021) Silver Nanoparticles and Their Antibacterial Applications. Int J Mol Sci. 22(13): 7202. doi: 10.3390/ijms22137202 Bukhari A, Ijaz I, Gilani E, Nazir A, Zain H, Saeed R, Alarfaji S S, Hussain S, Aftab R, Naseer Y (2021) Green Synthesis of Metal and Metal Oxide Nanoparticles Using Different Plants’ Parts for Antimicrobial Activity and Anticancer Activity: A Review Article. Coatings 11(11): 1374. https://doi.org/10.3390/coatings11111374 Chelliah P, Wabaidur S M, Sharma H P, Majdi H S, Smait D A, Najm M A, Iqbal A, Lai W-C (2023) Photocatalytic Organic Contaminant Degradation of Green Synthesized ZrO2 NPs and Their Antibacterial Activities. Separations, 10: 156. https://doi.org/10.3390/separations10030156 Chen L, Yang J, Li X, Liang T, Nie C, Xie F, Liu K, Peng X, Xie J (2020) Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells. J Nanobiotechnol. 18: 21. https://doi.org/10.1186/s12951-020-0581-0 Długosz O, Banach M, (2021) ZnO–SnO2–Sn nanocomposite as photocatalyst in ultraviolet and visible light. Appl Nanosci. 11: 1707–1719. https://doi.org/10.1007/s13204-021-01788-6. Haleem A, Javaid M, Singh R P, Rab S, Suman R (2023) Applications of nanotechnology in medical field: a brief review. Global Health Journal, 7: 70-77. https://doi.org/10.1016/j.glohj.2023.02.008 Haq S, Afsar H, Ali M B, Almalki M, Albogami B, Hedfi A (2021) Green Synthesis and Characterization of a ZnO-ZrO2 Heterojunction for Environmental and Biological Applications. Crystals,11(12):1502; https://doi.org/10.3390/cryst11121502 Hassaan M A, El-Nemr M A, Elkatory M R Ragab S, Niculescu V-C, Nemr A E (2023) Principles of Photocatalysts and Their Different Applications: A Review. Top Curr Chem (Z) 381: 31. https://doi.org/10.1007/s41061-023-00444-7 Hikal W M, Said-Al Ahl H A H, Bratovcic A, Tkachenko K G, Sharifi-Rad J, Kačániová M, Elhourri M, Atanassova M (2022) Banana Peels: A Waste Treasure for Human Being. Evid Based Complement Alternat Med., 2022: 7616452. doi: 10.1155/2022/7616452. eCollection 2022 Horti N C, Kamatagi M D, Nataraj S K, Wari M N, Inamdar S R (2020) Structural and optical properties of zirconium oxide (ZrO2) nanoparticles: effect of calcination temperature. Nano Express 1: 010022. DOI 10.1088/2632-959X/ab8684 Joudeh N, Linke D (2022) Nanoparticle classification, physicochemical properties, characterization, and applications: a comprehensive review for biologists. J Nanobiotechnol 20: 262. https://doi.org/10.1186/s12951-022-01477-8 Khairani I Y, Mínguez-Vega G, Doñate-Buendía C, Gökce B (2023), Green nanoparticle synthesis at scale: a perspective on overcoming the limits of pulsed laser ablation in liquids for high-throughput production.Phys. Chem. Chem. Phys., 25: 19380-19408. DOI: 10.1039/D3CP01214J Khan M, Shaik M R, Khan S T, Adil S F, Kuniyil M, Khan M, Al-Warthan A A, Siddiqui M R H, Tahir M N (2020) Enhanced Antimicrobial Activity of Biofunctionalized Zirconia Nanoparticles. ACS Omega 5: 1987–1996. Khattab E-S R, El Rehim S S A, Hassan W M I, El-Shazly T S (2021) Band Structure Engineering and Optical Properties of Pristine and Doped Monoclinic Zirconia (m-ZrO2): Density Functional Theory Theoretical Prospective. ACS Omega 6: 30061–30068. https://doi.org/10.1021/acsomega.1c04756 Kumar H, Bhardwaj K, Kuča K, Kalia A, Nepovimova E, Verma R, Kumar D (2020) Flower-based green synthesis of metallic nanoparticles:applications beyond fragrance. Nanomaterials , 10 (4): 766. https://doi.org/10.3390/nano10040766 Kumari N, Sareen S, Verma M, Sharma S, Sharma A, Sohal H S, Mehta S K, Park J, Mutreja V (2022) Zirconia-based nanomaterials: recent developments in synthesis and applications. Nanoscale Adv. 4(20): 4210–4236.doi: 10.1039/d2na00367h Lastra C J M P, Plou F J, Pérez-Lebeña E (2021) The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. Int. J. Mol. Sci. 22(9): 4642. https://doi.org/10.3390/ijms22094642 Mammari N, Lamouroux E, Boudier A, Duval R E (2022) Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles. Microorganisms, 10(2): 437. https://doi.org/10.3390/microorganisms10020437 Muthulakshmi N, Kathirvel A, Senthil M, Subramanian (2023) Green synthesis of zirconia nanoparticles and their characterization, anticancer activity and corrosion inhibition properties. R, J. Ind. Chem. Soc., 100 (10): 101076. https://doi.org/10.1016/j.jics.2023.101076 Nair G M, Sajini T, Mathew B (2022) Advanced green approaches for metal and metal oxide nanoparticles synthesis and their environmental applications. Talanta Open, 2022: 100080. https://doi.org/10.1016/j.talo.2021.100080 Narayanan K, Sakthivel C, Inbaraj P (2019) MgO-ZrO2 Mixed Nanocomposites: Fabrication Methods and Applications. Mater. Today Sustain., 3-4(2): 100007. 10.1016/j.mtsust.2019.100007 Nikolova M P, Chavali M S (2020) Metal Oxide Nanoparticles as Biomedical Materials. Biomimetics, 5(2), 27; https://doi.org/10.3390/biomimetics5020027 Ohiduzzaman M, Khan M N I, Khan K A, Paul B (2024) Biosynthesis of silver nanoparticles by banana pulp extract: Characterizations, antibacterial activity, and bioelectricity generation. Heliyon, 10: e25520. https://doi.org/10.1016/j.heliyon.2024.e25520 Ozdal M, Gurkok S (2022) Recent advances in nanoparticles as antibacterial agent. ADMET DMPK. 10(2): 115–129.doi: 10.5599/admet.1172 Sagadevan S, Podder J, Das I (2016) Hydrothermal synthesis of zirconium oxide nanoparticles and its Characterization. J Mater Sci: Mater Electron 27:5622–5627.DOI 10.1007/s10854-016-4469-6 Sandhya M, Ramasamy D, Sudhakar K, Kadirgama K, Haruna W S W (2021) Ultrasonication an intensifying tool for preparation of stable nanofluids and study the time influence on distinct properties of grapheme nanofluids – A systematic overview. Ultrason Sonochem. 73: 105479.doi: 10.1016/j.ultsonch.2021.105479 Shafey A M E (2020) Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: A review. Green Processing and Synthesis, 9: 304-339. https://doi.org/10.1515/gps-2020-0031 Sharma S (2022) Structure-activity relationship of some pentacoordinated Tributyltin(IV) complexes derived from sterically hindered Schiff bases of heterocyclic β-diketones. J. Ind. Chem. Soc., 99(6): 100464. https://doi.org/10.1016/j.jics.2022.100464 Sharma S, Kumar P, Jain A, Saxena S (2018) Synergy Between DFT Calculations and Experimental Studies on the Optimized Structures and the Antibacterial Potential of Some Novel Tetra- and Penta Coordinated Organic- Inorganic Hybrid Complexes of Titanium(IV). (2018), Appl. Organomet. Chem., 32(6): e4321. https://doi.org/10.1002/aoc.4321 Sharma S, Kumar R, P Kumar, Jain A, Saxena S (2019) New insights into the predicament of DFT assisted optimized energy, stability and distortions of optimized topologies of some novel complexes of Zirconium (IV) and enhancement of antimicrobial potential. Appl. Organomet. Chem., 33(10): e5080. https://doi.org/10.1002/aoc.5080 Shen L, Pang S, Zhong M, Sun Y, Qayum A, Liu Y, Rashid A, Xu B, Liang Q, Ma H, Ren X (2023) A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies. Ultrasonics Sonochemistry, 101: 106646. https://doi.org/10.1016/j.ultsonch.2023.106646 Sohail M, Baig N, Sher M, Jamil R, Altaf M, Akhtar S, Sharif M (2020) A Novel Tin-Doped Titanium Oxide Nanocomposite for Efficient Photo-Anodic Water Splitting. ACS Omega, 5: 6405−6413. https://dx.doi.org/10.1021/acsomega.9b03876. Szczyglewska P, Feliczak-Guzik A, Nowak I (2023) Nanotechnology–General Aspects: A Chemical Reduction Approach to the Synthesis of Nanoparticles. Molecules. 28(13): 4932. doi: 10.3390/molecules28134932 Talam S, Karumuri S R, Gunnam N (2012) Synthesis, Characterization, and Spectroscopic Properties of ZnO Nanoparticles. ISRN Nanotechnol. 372505. https://doi.org/10.5402/2012/372505 Tran T V, Nguyen D T C, Kumar P S, Din A T M, Jalil A A, Vo D-V N (2022) Green synthesis of ZrO2 nanoparticles and nanocomposites for biomedical and environmental applications: a review. Environ Chem Lett. 20(2): 1309–1331. Trieu, N D T, Nguyen, T T N, Nguyen, T T T, Nguyen, T C D, Tran T V (2023) A comparative study on the malachite green dye adsorption of chemically synthesized and green MgFe2O4 nanoparticles using gerbera floral waste extract. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-023-29779-w Verma D, Sharma S, Vashishtha M (2022) Evaluation of optimized molecular structure-antimicrobial and antioxidant efficacy relationship of Schiff Bases. Environ. Sci. Poll. Res. https://doi.org/10.1007/s11356-022-23633-1 Widoyanti A A E, Chaikong K, Rangsinth P, Saengratwatchara P, Leung G P-H, Prasansuklab A (2023) Valorization of Nam Wah Banana (Musa paradisiaca L.) Byproducts as a Source of Bioactive Compounds with Antioxidant and Anti-inflammatory Properties: In Vitro and In Silico Studies. Foods, 12(21): 3955; https://doi.org/10.3390/foods12213955 Yakout S M, Hassan H S (2014) Adsorption Characteristics of Sol Gel-Derived Zirconia for Cesium Ions from Aqueous Solutions. Molecules, 19: 9160-9172. doi:10.3390/molecules19079160 Ying S, Guan Z, Ofoegbu P C, Club P, Rico C, He F, Hong J (2022) Green synthesis of nanoparticles: Current developments and limitations.Environmental Technology & Innovation, 26: 102336. https://doi.org/10.1016/j.eti.2022.102336 Ying S, Guan Z, Ofoegbu P C, Clubb P, Rico C, He F, J Hong (2022) Green synthesis of nanoparticles: Current developments and limitations. Environ. Technol. Innov., 26: 102336. https://doi.org/10.1016/j.eti.2022.102336 Supplementary Files abstractforBioresourse2.jpg Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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8","display":"","copyAsset":false,"role":"figure","size":78335,"visible":true,"origin":"","legend":"\u003cp\u003eMechanism for the formation of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites using banana peel extract\u003c/p\u003e","description":"","filename":"8.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4946370/v1/1b4f060209973f55d9aa138a.jpg"},{"id":65074426,"identity":"8959b8c6-f879-4390-92d7-4b6e89cd7655","added_by":"auto","created_at":"2024-09-23 10:41:17","extension":"jpg","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":122481,"visible":true,"origin":"","legend":"\u003cp\u003eGreen-synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles exhibit antibacterial activity against four pathogens: (a) \u003cem\u003eK. pneumoniae, \u003c/em\u003e(b)\u003cem\u003e P. aeruginosa, \u003c/em\u003e(c) \u003cem\u003eB. subtilis, \u003c/em\u003eand\u003cem\u003e 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tropicalis\u003c/em\u003e\u003c/p\u003e","description":"","filename":"10.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4946370/v1/8abee32a27220a1477bdaba0.jpg"},{"id":65072995,"identity":"d7870959-a814-4903-8092-48f6a0e8acbf","added_by":"auto","created_at":"2024-09-23 10:17:17","extension":"jpg","order_by":11,"title":"Figure 11","display":"","copyAsset":false,"role":"figure","size":53703,"visible":true,"origin":"","legend":"\u003cp\u003eComparision of antioxidant activity of green synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites with bare ZrO\u003csub\u003e2 \u003c/sub\u003eat different concentrations showing by (a) DPPH and (b) ABST assay\u003c/p\u003e","description":"","filename":"11.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4946370/v1/532e022429a82924d1a11ce4.jpg"},{"id":65073449,"identity":"5d545bf8-6ec1-4011-be3d-62647301ce72","added_by":"auto","created_at":"2024-09-23 10:25:17","extension":"jpg","order_by":12,"title":"Figure 12","display":"","copyAsset":false,"role":"figure","size":88970,"visible":true,"origin":"","legend":"\u003cp\u003ePlausible mechanism for the applied dimensions of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites in the fields of adsorption, antioxidation and antimicrobial potential.\u003c/p\u003e","description":"","filename":"12.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4946370/v1/1d2a053dccd62aa6d5854694.jpg"},{"id":78387256,"identity":"cc360aa0-3ce8-4da4-a152-62f3e2412b78","added_by":"auto","created_at":"2025-03-12 16:55:07","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1849256,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4946370/v1/b803d7de-960a-4165-8d8e-1c0ef4b23208.pdf"},{"id":65073897,"identity":"069216d2-fc63-4c1b-bdd2-67bfbb4ddec6","added_by":"auto","created_at":"2024-09-23 10:33:17","extension":"jpg","order_by":4,"title":"","display":"","copyAsset":false,"role":"supplement","size":63292,"visible":true,"origin":"","legend":"","description":"","filename":"abstractforBioresourse2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4946370/v1/3196ea8159c9e15d486decb5.jpg"}],"financialInterests":"","formattedTitle":"Green Biogenic Synthesis of Sn-ZrO2 Nanocomposites Using Musa paradisiaca Peel Extract Under Sonication for Biological and Adsorption Efficacy","fulltext":[{"header":"Introduction","content":"\u003cp\u003eNanostructures are essential research tools in various fields, and nanotechnology is a rapidly expanding discipline with diverse applications in science and technology (Haleem et al. 2023). A great deal of research has been conducted on metal and metal oxide nanoparticles because of their exceptional electrical, optical, magnetic, catalytic, and medicinal properties\u0026nbsp;(Bukhari et al. 2021). Conventional techniques for creating metal and metal oxide nanoparticles use stabilizing and reducing chemical agents, which can be costly and harmful to the environment\u0026nbsp;(Nair\u0026nbsp;et al. 2022). \u0026nbsp;As a result, scientists are currently searching for different green synthesis techniques which minimize the hazardous substances used in the nanoparticles-producing process (Ying et al. 2022). In recent years, there has been extensive research on the eco-friendly, minimal-use method of green synthesis of metals and metal oxides nanoparticles employing plant extracts\u0026nbsp;(Shafey\u0026nbsp;et al. 2020). \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eZirconium oxide (ZrO\u003csub\u003e2\u003c/sub\u003e)\u003csub\u003e\u0026nbsp;\u003c/sub\u003enanoparticles are a highly valuable material in various fields, particularly in the dentistry (Kumari\u0026nbsp;et al. 2022). Their unique surface chemistry, high chemical and thermal stability, cost-effectiveness, non-hazardous and sustainable nature, clean photocatalytic properties, and impressive morphologies have significantly impacted both academia and industry\u0026nbsp;(Hassaan\u0026nbsp;et al. 2023). ZrO\u003csub\u003e2\u0026nbsp;\u003c/sub\u003eis a valuable material for water pollutant removal due to its high electron mobility, direct band gap, anisotropic growth, chemical stability, high photocatalytic efficiency, and ease of morphological control. Zirconium oxide is environmentally friendly and possesses outstanding biocompatibility\u0026nbsp;(Bannunah 2023). The usefulness of zirconia is limited by two intrinsic properties: a high band gap and quick electron-hole pair recombination (Khattab\u0026nbsp;et al. 2021). The synthesis of metal nanoparticles (Ag, Au, Sn, Cu, Ru, Pd, etc.) doped on metallic oxide surfaces has recently attracted a lot of interest in the fields of material science and nanotechnology due to its significantly enhanced applications in a variety of fields (Szczyglewska et al. 2023). \u0026nbsp;\u003c/p\u003e\n\u003cp\u003eFurthermore, studies show that tin-doping controls the cytotoxicity of zirconia nanoparticles by killing human cancer cells alone while leaving healthy cells unharmed\u0026nbsp;(Barbasz et al. 2024). It was recently discovered that Sn-ZrO\u003csub\u003e2\u003c/sub\u003e generate a reactive oxygen species (ROS) that can destroy microbial colonies in the absence of light irradiation (Mammari et al. 2022). Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites formation is a good choice because of their unique optical-physiological and biological properties, ease of synthesis at a low cost, high availability, and low toxicity.\u003c/p\u003e\n\u003cp\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites have been synthesized using a variety of methods, including the thermal decomposition, sol-gel process, co-precipitation and electrochemical oxidation process (Narayanan et al.2019;\u0026nbsp;Bharti and\u0026nbsp;Sadhu 2022). Although, some of these physio-chemical methods not only violate environmental regulations but also cause poor dispersibility and irreversible nanoparticle agglomeration, which reduces biological activity\u0026nbsp;(Joudeh and\u0026nbsp;Linke 2022). Consequently, the synthesis of different nanomaterials utilizing plant extracts has recently caught the interest of researchers as a means of getting around these limitations (Ying et al. 2022). However, less research has been done on the synthesis of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites utilizing plant extracts.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe synthesis of nanocomposites typically requires two or more phases and a lengthy reaction procedure, although plant mediated synthesis support green approaches (Ying et al.2022). The ultrasonic energy used as a non-conventional source act as a workable and environmentally beneficial alternative for material production\u0026nbsp;(Shen et al. 2023). Some research indicates that the cavitational action of ultrasonic waves during nanomaterial manufacturing reduced the reaction time and produced smaller particles\u0026nbsp;(Sandhya\u0026nbsp;et al. 2021). During cavitation, bubbles form and burst, rapidly producing large quantities of non-aggregated nanoparticles\u0026nbsp;(Khairani\u0026nbsp;et al. 2023).\u003c/p\u003e\n\u003cp\u003eIn light of the many uses and paucity of research on plant-mediated nanocomposites synthesis and as part of our continuing endeavour on the designing and green synthesis of functional nanocomposites (Bhardwaj et al. 2021; Bhardwaj et al. 2019), we have shown here for the first time a green banana peel extract assisted synthesis of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites under sonication. Biomolecules included in banana peel extract serve as stabilizing and reducing agents in the synthesis of nanomaterials (Ohiduzzaman et al. 2024; Bao et al. 2021).\u003c/p\u003e"},{"header":"Materials and Methods","content":"\u003cp\u003eBananas were collected from the local area of Jaipur district, Rajasthan, India. The chemicals used in this study were zirconyl nitrate [ZrO(NO\u003csub\u003e3\u003c/sub\u003e)\u003csub\u003e2\u003c/sub\u003e.xH\u003csub\u003e2\u003c/sub\u003eO], stannous chloride (SnCl\u003csub\u003e4\u003c/sub\u003e), ethanol, ZrO\u003csub\u003e2\u003c/sub\u003e, 2, 2-Diphenyl1-picrylhydrazyl (DPPH), ABTS (2,2\u0026rsquo;-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)), etc. were purchased from Merck Chemical Company (Darmstadt, Germany) and used as such without further purification.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacterization methods and Instruments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eUV-Vis, FTIR, XRD, and SEM-EDS studies were used to characterize Sn-ZrO\u003csub\u003e2\u0026nbsp;\u003c/sub\u003enanocomposites that were green-synthesised. Using a quartz cuvette filled with de-ionized water as a reference, UV-Vis NIR spectrophotometer was employed. FTIR spectra were captured in a spectral range of 4000-400 cm\u003csup\u003e-1\u003c/sup\u003e using KBr pellets.\u0026nbsp;The X-ray diffractometer, which was outfitted with a CuK\u0026alpha; radiation (\u0026lambda;=1.54060 \u0026Aring;) was used to perform XRD measurements. Surface morphology and form of the nanocomposites were evaluated using FE-SEM. The FE-SEM and EDS detector are connected so that elements included in nanoparticles can be identified and their chemical composition can be examined. The ultrasound assisted reactions were performed using an ultrasonic processor probe system. For ten minutes, the operating conditions involved a 30-second on and 30-second off pulse with 50% amplitude.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation of \u003cem\u003eMusa paradisiaca\u0026nbsp;\u003c/em\u003e(Banana)\u003cem\u003e\u0026nbsp;\u003c/em\u003epeel extract\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAfter gathering the \u003cem\u003eMusa paradisiaca\u003c/em\u003e peels, they were carefully cleaned under running water to get rid of any associated dust particles, and then dried. About 20 gm of dried banana\u003cem\u003e\u0026nbsp;\u003c/em\u003epeel were powdered, and 100 ml of deionized water was added. In order to prepare the peel extract, the mixture was heated at 80\u0026deg;C for ten minutes. After allowing the combination to cool to room temperature, Whatman No. 1 filter paper was used to filter the mixture. After the residue was eliminated, the filtrate was utilized to create nanocomposites.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSynthesis of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites using \u003cem\u003eMusa paradisiaca\u0026nbsp;\u003c/em\u003epeel extract\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTo create Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites in an environmentally friendly manner, a cylindrical glass vessel containing a precursor solution of 0.1M zirconyl nitrate and 15 ml deionized water was subjected to a 10-minute sonication period. \u0026nbsp;Subsequently, a 1 mM stannous chloride solution was applied dropwise while being continuously sonicated to a 25 ml banana peel extract. The solution turned gray after ten minutes as a result of surface plasmon resonance (SPR) activation, which showed that Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites were forming. The precipitation of Sn-ZrO₂ nanocomposites was completed by centrifuging the solution for 20 minutes at 6000 rpm. After three water washes to eliminate byproducts, the precipitate was dried in an oven at 80 \u0026deg;C for an entire night, and it was then annealed for two hours at 500 \u0026deg;C.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntimicrobial activities\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe traditional well-diffusion method was employed to examine the antimicrobial potential of produced Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites, as previously described (Sharma et al. 2018; Sharma et al. 2019; Sharma et al. 2022). Two distinct fungi, \u003cem\u003eC. albicans\u003c/em\u003e and \u003cem\u003eC. tropicalis\u003c/em\u003e, were evaluated for their antifungal activity, while pathogenic microorganisms classified as Gram-negative (\u003cem\u003eK. pneumonia\u003c/em\u003e \u0026amp; \u003cem\u003eP. aeruginosa\u003c/em\u003e) and Gram-positive (\u003cem\u003eB. subtilis\u003c/em\u003e \u0026amp; \u003cem\u003eS. aureus\u003c/em\u003e) were tested for their antibacterial activity. Every strain was evenly swabbed onto the sterilized nutritional agar petriplates using cotton swabs. Then, using a sterile plastic rod, 8 mm diameter wells were pierced into the inoculation plates. Four concentrations (20, 40, 60, and 80 \u0026micro;g/ml) of produced Sn-ZrO\u003csub\u003e2\u0026nbsp;\u003c/sub\u003enanocomposites were added, accordingly, to the specified wells\u0026nbsp;using a micropipette. After incubating for 24 hours at 37\u0026deg;C, the antimicrobial activity was assessed by measuring the diameter of the inhibitory zone in millimeters (mm) using a standard scale. The antimicrobial activity of the resulting Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was compared with that of ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles in order to measure the effectiveness of Sn loading in nanocomposites and also compared with reference standard.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntioxidant activity\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe antioxidant potential of the green produced ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles and Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was evaluated and compared with standard using DPPH and ABTS tests. \u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDPPH\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003eassay\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAccording to a previous approach (Verma et al. 2022), the antioxidant activity of green synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles was examined using DPPH assay. One milliliter of DPPH solution (0.1 millimeter of DPPH in methanol) was filled with different concentrations of synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles, and the test tubes were labeled appropriately. After shaking the reaction mixture, it was left at room temperature for 30 minutes in a dark environment. At 517 nm, the absorbance was measured spectrophotometrically. \u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eABTS assay\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe ABTS free radical scavenging activity of ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles and green synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was examined using the previously reported methodology, with some minor adjustments (Haq et al. 2021). By reacting 2.45mM of potassium persulfate (K\u003csub\u003e2\u003c/sub\u003eS\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e8\u003c/sub\u003e) with 7.mM of the ABTS stock solution, ABTS radical cation (ABTS\u003csup\u003e+.\u003c/sup\u003e) stock solution was prepared. The absorbance at 734 nm was measured following an overnight incubation period at room temperature. ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles and synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites were added individually with ABTS\u003csup\u003e+.\u003c/sup\u003e at varying concentrations, and they were once more incubated for 15 minutes in a dark environment. As a control solution, ABTS reagent was employed without sample.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eBatch Adsorption Studies:\u0026nbsp;\u003c/strong\u003eMalachite green (MG) dye was made as a stock solution (100 ppm) in deionized water, and subsequently diluted to yield a range of samples from 1 to 12 ppm. The effects of various parameters on the removal of MG dye using Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites during the batch adsorption method are investigated. These parameters include adsorbent dosage (5, 10, 15, 20, 25, 30, 35 and 40 mg), contact time (5, 10, 15, 20, 25, 30 min), pH (2 to 12), and temperature (303, 323 and 353 K).\u003c/p\u003e\n\u003cp\u003eThe adsorption capacity of MG dye was determined using Equation 1, while the removal efficiency (%) was calculated with Equation 2.\u003c/p\u003e\n\u003cp\u003e(q\u003csub\u003ee\u003c/sub\u003e) = (C\u003csub\u003eo\u003c/sub\u003e-C\u003csub\u003ee\u003c/sub\u003e) V/M \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Equation (1)\u003c/p\u003e\n\u003cp\u003eRemoval (%) = (C\u003csub\u003eo\u003c/sub\u003e-C\u003csub\u003ee\u003c/sub\u003e) / C\u003csub\u003eo\u003c/sub\u003e \u0026times;\u0026nbsp;100 \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Equation (2)\u003c/p\u003e\n\u003cp\u003ewhere M (mg) is the mass of the Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites, V (L) is the volume of MG dye obtained in an aqueous solution, and C\u003csub\u003eo\u003c/sub\u003e (mg/L) and C\u003csub\u003ee\u003c/sub\u003e (mg/L) are the initial and equilibrium concentrations, respectively, of MG dye.\u003c/p\u003e"},{"header":"Results and discussion","content":"\u003cp\u003e\u003cstrong\u003eUV\u0026ndash;Vis Spectrophotometer:\u0026nbsp;\u003c/strong\u003eFig. 1, comprises the room-temperature optical absorption of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites in the range of 200\u0026ndash;800 nm. \u0026nbsp;The sharp absorption peak seen at around 260\u0026thinsp;nm can be attributed to ZrO\u003csub\u003e2.\u0026nbsp;\u003c/sub\u003e (Chelliah et al. 2023). The synthesized nanocomposites demonstrated absorbance band from 280-550 nm shows deposition of Sn nanoparticles on the surface of nanocomposites successfully (Sohail et al. 2020).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFTIR analysis\u003c/strong\u003e: Fig. 2 reveals the FTIR spectrum of green synthesis Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites. The characteristic broad peaks of OH groups are found at 3428.71 and 1628.34 cm\u003csup\u003e-1\u003c/sup\u003e corresponds to the water molecules that have been absorbed on the surface of the nanoparticles. The stretching and bending vibrational modes of the water molecule are responsible for these broad peaks (Sagadevan et al. 2016). The weak bands at 2922.36, 2852.34, and 1384.31 cm\u003csup\u003e\u0026minus;1\u003c/sup\u003ewere associated with the vibrations of organic residuals\u0026nbsp;(Alharthi et al. 2020). The absorption band that was most prominent and sharpest emerged at 1121.69 and 617.74, cm\u003csup\u003e\u0026minus;1\u003c/sup\u003e potentially indicating a correlation with the Zr\u0026ndash;O bonds. The bending vibration of hydroxyl groups attached to ZrO\u003csub\u003e2\u003c/sub\u003e is responsible for the peaks at 1121.69 cm\u0026minus;1 and 617.74 cm\u003csup\u003e\u0026minus;1\u003c/sup\u003e. The Zr\u0026ndash;O vibration is represented by the band at 617.74 cm\u003csup\u003e\u0026minus;1\u003c/sup\u003e, and the breadth of the band indicates that the ZrO\u003csub\u003e2\u003c/sub\u003e powders are nano crystals (Yakout et al. 2014).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eXRD:\u0026nbsp;\u003c/strong\u003eX-ray diffraction measurements were used to investigate the crystallinity and phase form of the synthesised nanomaterials. Fig. 3 demonstrate the XRD pattern of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and bare ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles. The diffraction peaks in a wide range angle of 2\u0026theta; are at 30.64\u0026deg;, 35.22\u0026deg;, 50.26\u0026deg;, and 60.17\u0026deg; corresponds to the crystal planes of (101), (110), (112), and (211), respectively, attributed to the preparation of monoclinic phase of ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles (Horti et al. 2020) (JCPDS card no. 01‐075‐2550). Comparison of XRD pattern reveals the appearance of strong intense peaks at 2\u0026theta; = 30.10\u0026deg; and 35.05\u0026deg; to be (101) and (110) crystal planes of Sn doping on ZrO\u003csub\u003e2\u003c/sub\u003e as shown in Fig. 3 (Długosz et al. 2021). The XRD patterns do not exhibit distinctive peaks associated with Sn, indicating that all Sn atoms have successfully integrated into the ZrO\u003csub\u003e2\u003c/sub\u003e lattice. Additionally, the absence of any other diffraction peaks confirms the synthesized nanocomposites high purity.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSEM and EDS analyses\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe detailed morphology, particle size, and shape of the biomorphic Sn-ZrO₂ nanocomposites were examined using SEM analysis. The results revealed a non-uniform distribution of spherical tin particles on the surface of zirconia nanoparicles. The average size of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites is 25nm-50nm (Fig. 4a, b). The elemental composition of the Sn-ZrO₂ nanocomposites was determined by EDS analysis, confirming the presence of zirconium (Zr), tin (Sn), and oxygen (O) as shown in Fig. 5. Additional presence of elements (calcium) is also observed due to the presence of some phytochemicals from extract. \u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdsorption performance of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites:\u0026nbsp;\u003c/strong\u003eThis experimental work was conducted using a 10 ppm MG dye solution. 30 mg of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites were put to a beaker containing 20ml of MG dye solution. The mixture is shaken constantly for 25 minutes or until equilibrium is reached. The Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was used to study the adsorption of MG dye from an aqueous solution at different dye concentrations, contact times, dosages of the adsorbent, pH levels, and temperatures. The absorbent was changed from 5 to 40 mg/L while all other parameters remained same. The pH was adjusted to a range between 3 and 10 ppm using 0.1 M HCl and 0.1 M NaOH solutions. The shake took longer than five minutes to complete. The adsorbate solution was taken out and filtered once the allotted time had elapsed in order to isolate the adsorbent. Following filtering, a UV-Vis spectrophotometer that had previously been calibrated was used to measure the solution\u0026apos;s concentration at 615 nm.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlterations in adsorbent dose:\u0026nbsp;\u003c/strong\u003eDoses are crucial during the adsorption process. To investigate the effect of the Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites dosage on the adsorption of MG dye (10 mg/L), adsorbent dosages ranging from 5 mg to 40 mg were used. The removal effectiveness was increased from 5 to 40 mg of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites, as shown in Fig. 6(a). This is because larger surface areas and more active sites are present in adsorbent. It was shown that the Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites had an optimal dose of 30 mg. Once the optimal adsorbent dose has been reached, the removal efficiency falls.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eVariation with pH:\u0026nbsp;\u003c/strong\u003eSince pH influences the surface charge of the adsorbate (MG dye) and Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites adsorbent, it plays a significant part in adsorption processes. The influence of pH on the effectiveness of nanocomposites\u0026apos; removal of MG dye is shown in Fig. 6b. To adjust the required pH, 0.1 M sodium hydroxide or 0.1 M hydrochloric acid was used. \u0026nbsp;As the pH rose from 8 to 10, the removal efficiency significantly declined after increasing from 3 to 8.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEffect of temperature\u003c/strong\u003e: \u0026nbsp; Temperature is thought to be a significant element that affects how well the MG is adsorbed and removed. At various temperatures between 303 and 353 K, the adsorbent dosage (30 mg/L), pH (8), contact period is 25 min, and constant agitation speed (450 rpm) were maintained in order to assess the impact of temperature on MG dye adsortion. As the temperature was elevated from 303 to 353 K, the rate of MG adsorption on Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites decreased, as shown in Fig. 7(a). This suggests that the adsorption process was somewhat endothermic.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eInfluence of contact time:\u0026nbsp;\u003c/strong\u003eAnalyzing the effect of the time of contact is important because the results of this type of study provide basic information about the rate at which the adsorption process reaches equilibrium. The effect of altering the contact time within the range of 15 to 20 minutes on the adsorption capacity was examined, keeping all other parameters fixed. The experiment started with fast dye adsorption, which gradually slowed down when the equilibrium condition was approached after around 15-20 minutes, based on the results displayed in Fig 7(b).\u003c/p\u003e\n\u003cp\u003eFig. 8 outlines a proposed mechanism for the formation of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites. Amorphous hydrous oxide precipitates are formed when zirconium nitrate is hydrolyzed in aqueous circumstances, resulting in Zr(OH)\u003csub\u003e4\u003c/sub\u003e, which is naturally unstable and prone to condensation processes (Muthulakshmi et al. 2023; Chelliah et al. 2023). These condensation reactions are catalyzed by the hydroxyl groups present in banana peel extract, which are renowned for their antioxidant properties.\u003c/p\u003e\n\u003cp\u003eMeanwhile, in the aqueous solution, tin chloride quickly separates into tin and chloride ions. As seen in the Fig. 8, the reducing phytochemicals in the extract bind and cap the tin ions to produce stable nanoparticles. The primary organic substances accountable for this process include the carotenoids and other phytonutrients found in banana peel extract, as well as anthocyanins, delphinidin, cyaniding, and catecholamines (Hikal et al 2022). The resultant Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites are then subjected to a 500\u0026deg;C calcination process in order to remove remaining phytochemical residue and water molecules.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntimicrobial activities of synthesised\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eGreen synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles were tested against Gram positive and Gram negative bacteria, including \u003cem\u003eB. subtilis\u003c/em\u003e, \u003cem\u003eS. aureus\u003c/em\u003e, \u003cem\u003eK. pneumonia\u003c/em\u003e and \u003cem\u003eP. aeruginosa\u003c/em\u003e. Fig. 9 illustrates the zone of inhibition observed in bacteria due to the synthesized Sn-ZrO₂ nanocomposites at four different concentrations, compared to ZrO₂ nanoparticles. At an 80 \u0026micro;g/mL concentration of Sn-ZrO₂ nanocomposites, the largest zone of inhibition was recorded for \u003cem\u003eK. pneumoniae\u003c/em\u003e (21 mm), followed by \u003cem\u003eB. subtilis\u003c/em\u003e (20 mm). The smallest inhibitory zone was observed for \u003cem\u003eS. aureus\u003c/em\u003e (5 mm) at a 20 \u0026micro;g/mL concentration of Sn-ZrO₂ nanocomposites. Through conducting studies with varying concentrations of nanocomposites, we discovered that the zone of inhibition rises as the concentration of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites increases (Table 1). Bacterial membrane integrity is further compromised by lipid peroxidation, which is influenced by elevated levels of ROS. As the concentration of nanoparticles rises, the breakdown of the bacterial cell wall causes the bacteria to die (Ozdal et al. 2022). The likelihood that the nanoparticles will penetrate and harm the bacterial membrane increases with their size (Ozdal et al. 2022). The passage of nanoparticles across the plasma membrane has been facilitated by the presence of ion channels and transporter protein (Chen et al. 2020). The increase level of ROS affects lipid peroxidation in bacteria which further influence the integrity of bacterial membrane. The destruction of the bacterial cell wall results bacterial death with increase in concentration of nanoparticles (Juan et al. 2021).\u003c/p\u003e\n\u003cp\u003eIn comparison to ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles, the Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites exhibits a larger zone of inhibition. When tin ions are released from a Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites, it strengthens its ability to connect with bacterial enzymes that inactivate bio cells by breaking through their cell walls and damaging the bacteria. (Nikolova et al. 2020).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1:\u003c/strong\u003e Zone of inhibition (mm) at various concentrations of green-synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles.\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" align=\"\" width=\"678\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"121\" rowspan=\"2\" valign=\"top\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"557\" colspan=\"9\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eZone of Inhibition in mm\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"268\" colspan=\"5\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003eGram negative bacteria\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"289\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003eGram positive\u003c/strong\u003e \u003cstrong\u003ebacteria\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"121\" rowspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eConcentration\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"143\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003e\u003cem\u003eK. pneumoniae\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"126\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003e\u003cem\u003eP. aeruginosa\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"144\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003e\u003cem\u003eB. subtilis\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"144\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003e\u003cem\u003eS. aureus\u0026nbsp;\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"58\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"84\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"73\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"66\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp; ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"78\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"65\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"79\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"121\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e20\u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"58\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"84\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"73\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"66\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"78\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"65\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"79\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"121\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e40 \u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"58\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"84\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"73\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"66\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"78\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"65\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"79\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"121\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e60 \u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"58\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"84\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"73\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e15\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"66\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"78\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"65\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"79\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"121\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e80 \u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"58\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"84\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"73\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"66\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"78\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"65\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"79\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eSimilarly, ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles and green-synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites were tested for their antifungal properties against two different fungi, \u003cem\u003eC. albicans\u003c/em\u003e and \u003cem\u003eC. tropicalis\u003c/em\u003e, as shown in Fig. 10. The synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites had strong antifungal activity against \u003cem\u003eC. albicans\u003c/em\u003e, but only weak activity against \u003cem\u003eC. tropicalis\u003c/em\u003e. As shown in Table 2, a relatively small amount of green Sn-ZrO₂ nanocomposites (20 \u0026micro;g/mL) was sufficient to disrupt the fungal cell membrane and ultimately kill the fungi. Comparing the antifungal activity of the synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites to ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles, it demonstrates a greater zone of inhibition of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites much like the antibacterial assay. The higher antifungal activity of synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was partly attributed to the smaller particle size attained through sonication. This is because smaller particles have a larger surface to volume ratio, which permits more drug molecules to be adsorbed on the surface. These molecules are anticipated to act as a powerful agent in breaking down cell walls (Bruna et al. 2021). The findings show that, in comparison to ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles, synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites is a more effective antimicrobial agent with a greater potential to kill germs.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2:\u003c/strong\u003e Zone of inhibition (mm) at different concentrations of green synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles against \u003cem\u003eC. albicans and C. tropicalis\u003c/em\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"601\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"120\" rowspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eConcentration\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"481\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003eZone of Inhibition in mm\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"222\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003e\u003cem\u003eC. albicans\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"259\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003e\u003cem\u003eC. tropicalis\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"122\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"126\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u003cstrong\u003eZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"133\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"120\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e20\u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"100\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"122\" valign=\"top\"\u003e\n \u003cp\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"126\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"133\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"120\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e40 \u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"100\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"122\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"126\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;4.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"133\" valign=\"top\"\u003e\n \u003cp\u003e6.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"120\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e60 \u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"100\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 9.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"122\" valign=\"top\"\u003e\n \u003cp\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"126\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"133\" valign=\"top\"\u003e\n \u003cp\u003e8.3\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"120\" valign=\"top\"\u003e\n \u003cp align=\"center\"\u003e80 \u0026micro;g/ml\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"100\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;11.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"122\" valign=\"top\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"126\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"133\" valign=\"top\"\u003e\n \u003cp\u003e10.4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eNonetheless, \u003cem\u003eMusa paradisiaca\u003c/em\u003e has long been a well-known traditional herb used in medicinal field. Its exceptional biological potentials are an added benefit, and combined with the potent biological properties of tin and ZrO\u003csub\u003e2\u003c/sub\u003e, these properties would greatly encourage the green synthesis of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites utilizing this well-known herb. Previous research has also shown that biosynthesised nanoparticles have potent antimicrobial efficacy than pure chemically synthesized nanoparticles. (Khan et al. 2020).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAntioxidant activity of\u003c/strong\u003e \u003cstrong\u003eSn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFree radicals are neutralized by an antioxidant substance, which halts the oxidation process. Green synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles were tested against DPPH at various doses in an antioxidant assay (Fig. 11a). Significantly, Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites showed higher radical scavenging activity than ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles, indicating that the inclusion of tin particles improves the antioxidant nature of nanocomposites by effectively separating electron-hole pairs (Tran et al. 2022). The findings demonstrated that the Sn-ZrO\u003csub\u003e2\u0026nbsp;\u003c/sub\u003enanocomposites inhibits DPPH activity in a dose-dependent manner, means that radical scavenging assay enhances with the increase in concentration of the nanocomposites. \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe antioxidant experiment was conducted against ABTS using varying quantities of ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles and green synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites (Fig. 11b). Peels of \u003cem\u003eMusa paradisiaca\u003c/em\u003e are rich in bioactive substances (polyphenols and oils containing essential fatty acids) that may be able to be scavenged because of their hydroxyl groups (Widoyanti et al. 2023). Similar to DPPH scavenging, dose-dependent action for ABTS was also observed. At greater concentrations, the green-synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites demonstrated stronger activity in blocking the ABTS radical than ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles.\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eMusa paradisiaca\u003c/em\u003e peel extract contains phytochemicals, which are well-known for their antioxidant qualities. Tin and ZrO\u003csub\u003e2\u003c/sub\u003e\u0026apos;s inherent antioxidant properties also contributes to the extract\u0026apos;s increased scavenging activity, making it more powerful and active. Based on the aforementioned findings, it can be concluded that the environmentally friendly green Sn-ZrO₂ nanocomposites, synthesized using \u003cem\u003eMusa paradisiaca\u003c/em\u003e peel extract under ultrasound irradiation, is a more viable option for antioxidant drugs compared to ZrO₂ nanoparticles and serves as a superior alternative to chemically synthesized options. \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePlausible mechanism responsible for the different applied dimensions of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites \u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFig. 12 illustrates a plausible mechanism for the application of Sn-ZrO₂ nanocomposites in the adsorption potential, antimicrobial activity and antioxidant activity. Through metabolic processes, biogenic Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites most likely interacts with the membranes and cell walls of bacteria. They are more likely to produce ROS, which can lead to a variety of problems, including the deactivation of vital enzyme and protein functions. Consequences include rupture of the cell membrane, cytoplasmic leakage, damage to proteins, DNA, and mitochondria, and finally, cell death (Kumar et al. 2020). The Sn-ZrO\u003csub\u003e2\u0026nbsp;\u003c/sub\u003enanocomposites, which was created from banana peel extract, demonstrated superior efficacy in scavenging a range of ROS when tested for antioxidant activity using the ABTS and DPPH free radical assay\u0026nbsp;(Kumar et al. 2020).\u0026nbsp;The adsorption mechanism of MG dye on nanocomposites surfaces is influenced by various interactions, which are affected by specific phytochemicals. These include \u0026pi;\u0026ndash;\u0026pi; stacking interactions between the surface of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites and aromatic rings of MB, hydrogen bonding interactions between the functional groups on the nanocomposites surface and the nitrogen atoms of MG dye, and electrostatic interactions between the positively charged nitrogen atoms present on the dye and the negatively charged functional groups on the nanocomposites surface (Trieu et al. 2023).\u0026nbsp;\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eThis work presents the synthesis of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites by means of a straightforward, economical, and environmentally sustainable method that employs \u003cem\u003eMusa paradisiaca\u003c/em\u003e peel extract as a bio-reductant under ultrasonic irradiation. The reduction and stability of the nanocomposites were accomplished using the aqueous peel extract that contained phytoconstituents. The newly synthesized Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was characterized by using FTIR, UV-Vis, XRD, and SEM-EDS techniques. The Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites was tested for antimicrobial and antioxidant properties, and dose-dependent variation was observed. When compared to ZrO\u003csub\u003e2\u003c/sub\u003e nanoparticles, the green-synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites shown superior biological activities that were comparable to benchmark. It is observed that Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites possess excellent adsorption capacity than ZrO\u003csub\u003e2\u003c/sub\u003e for MG dye. The current sonochemical synthesis process has many benefits, including low temperature, fast reaction times, and good surface dispersibility of the doped metal, which results in smaller particles with higher yields.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors are highly thankful to MRC, MNIT Jaipur for providing spectroscopic facilities, and Applied Seminal Jaipur for doing antimicrobial and antioxidative studies of the samples.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026apos; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eS.S. - conceptualization, methodology, formal analysis, investigations, resources, supervision, writing original draft, visualization; D.B.\u0026mdash;\u0026nbsp;conceptualization, methodology, formal analysis, investigations, resources, data curation, supervision, visualization, writing \u0026mdash; review and editing. All authors contributed to the manuscript and approved the final version for publication.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNone\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData Availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll supporting data are available in the article.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval\u003c/strong\u003e Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to participate\u003c/strong\u003e Not applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e All authors approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e The authors declare no competing interests.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eAlharthi F A, Alghamdi A A, Al-Zaqri N, H S. Alanazi, A A Alsyahi, A E Marghany, N Ahmad (2020) Facile one-pot green synthesis of Ag\u0026ndash;ZnO Nanocomposites using potato peeland their Ag concentration dependent photocatalytic properties. Sci Rep 10: 20229. https://doi.org/10.1038/s41598-020-77426-y\u003c/li\u003e\n\u003cli\u003eBannunah A M (2023) Biomedical Applications of Zirconia-Based Nanomaterials: Challenges and Future Perspectives. Molecules, 28(14): 5428. https://doi.org/10.3390/molecules28145428\u003c/li\u003e\n\u003cli\u003eBao Y, He J, Song K, Guo J, Zhou X, Liu S (2021) Plant-Extract-Mediated Synthesis of Metal Nanoparticles. J. Chem., 2021:| 6562687. https://doi.org/10.1155/2021/6562687\u003c/li\u003e\n\u003cli\u003eBarbasz A M, Dyba B (2024) Direct Interaction of Zirconia Nanoparticles with Human Immune Cells. Biophysica, 4(1): 83-91; https://doi.org/10.3390/biophysica4010006\u003c/li\u003e\n\u003cli\u003eBhardwaj D, Singh A, Singh R (2019). Eco-compatible sonochemical synthesis of 8-aryl-7,8-dihydro-[1,3]-dioxolo[4,5-g]quinolin-6(5H)-ones using green TiO2. Heliyon. 5 (2), e01256. https://doi.org/10.1016/j.heliyon.2019.e01256.\u003c/li\u003e\n\u003cli\u003eBhardwaj D, Singh R (2021) Green biomimetic synthesis of Ag\u0026ndash;TiO\u003csub\u003e2\u003c/sub\u003e nanocomposite using Origanum majorana leaf extract under sonication and their biological activities. Bioresour. Bioprocess. 8: 1. https://doi.org/10.1186/s40643-020-00357-z\u003c/li\u003e\n\u003cli\u003eBharti K, Sadhu K K (2022) Syntheses of metal oxide-gold nanocomposites for biological applications. Res. Chem. 4:100288.https://doi.org/10.1016/j.rechem.2022.100288\u003c/li\u003e\n\u003cli\u003eBruna T, Maldonado-Bravo F, Jara P, Caro N (2021) Silver Nanoparticles and Their Antibacterial Applications. Int J Mol Sci. 22(13): 7202. doi: 10.3390/ijms22137202\u003c/li\u003e\n\u003cli\u003eBukhari A, Ijaz I, Gilani E, Nazir A, Zain H, Saeed R, Alarfaji S S, Hussain S, Aftab R, Naseer Y (2021) Green Synthesis of Metal and Metal Oxide Nanoparticles Using Different Plants\u0026rsquo; Parts for Antimicrobial Activity and Anticancer Activity: A Review Article. Coatings 11(11): 1374. https://doi.org/10.3390/coatings11111374\u003c/li\u003e\n\u003cli\u003eChelliah P, Wabaidur S M, Sharma H P, Majdi H S, Smait D A, Najm M A, Iqbal A, Lai W-C (2023) Photocatalytic Organic Contaminant Degradation of Green Synthesized ZrO2 NPs and Their Antibacterial Activities. Separations, 10: 156. https://doi.org/10.3390/separations10030156\u003c/li\u003e\n\u003cli\u003eChen L, Yang J, Li X, Liang T, Nie C, Xie F, Liu K, Peng X, Xie J (2020) Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells. J Nanobiotechnol. 18: 21. https://doi.org/10.1186/s12951-020-0581-0 \u003c/li\u003e\n\u003cli\u003eDługosz O, Banach M, (2021) ZnO\u0026ndash;SnO2\u0026ndash;Sn nanocomposite as photocatalyst in ultraviolet and visible light. Appl Nanosci. 11: 1707\u0026ndash;1719. https://doi.org/10.1007/s13204-021-01788-6.\u003c/li\u003e\n\u003cli\u003eHaleem A, Javaid M, Singh R P, Rab S, Suman R (2023) Applications of nanotechnology in medical field: a brief review. Global Health Journal, 7: 70-77. https://doi.org/10.1016/j.glohj.2023.02.008\u003c/li\u003e\n\u003cli\u003eHaq S, Afsar H, Ali M B, Almalki M, Albogami B, Hedfi A (2021) Green Synthesis and Characterization of a ZnO-ZrO2 Heterojunction for Environmental and Biological Applications. Crystals,11(12):1502; https://doi.org/10.3390/cryst11121502\u003c/li\u003e\n\u003cli\u003eHassaan M A, El-Nemr M A, Elkatory M R Ragab S, Niculescu V-C, Nemr A E (2023) Principles of Photocatalysts and Their Different Applications: A Review. Top Curr Chem (Z) 381: 31. https://doi.org/10.1007/s41061-023-00444-7\u003c/li\u003e\n\u003cli\u003eHikal W M, Said-Al Ahl H A H, Bratovcic A, Tkachenko K G, Sharifi-Rad J, Kač\u0026aacute;niov\u0026aacute; M, Elhourri M, Atanassova M (2022) Banana Peels: A Waste Treasure for Human Being. Evid Based Complement Alternat Med., 2022: 7616452. doi: 10.1155/2022/7616452. eCollection 2022\u003c/li\u003e\n\u003cli\u003eHorti N C, Kamatagi M D, Nataraj S K, Wari M N, Inamdar S R (2020) Structural and optical properties of zirconium oxide (ZrO2) nanoparticles: effect of calcination temperature. Nano Express 1: 010022. DOI 10.1088/2632-959X/ab8684\u003c/li\u003e\n\u003cli\u003eJoudeh N, Linke D (2022) Nanoparticle classification, physicochemical properties, characterization, and applications: a comprehensive review for biologists. J Nanobiotechnol 20: 262. https://doi.org/10.1186/s12951-022-01477-8\u003c/li\u003e\n\u003cli\u003eKhairani I Y, M\u0026iacute;nguez-Vega G, Do\u0026ntilde;ate-Buend\u0026iacute;a C, G\u0026ouml;kce B (2023), Green nanoparticle synthesis at scale: a perspective on overcoming the limits of pulsed laser ablation in liquids for high-throughput production.Phys. Chem. Chem. Phys., 25: 19380-19408. DOI: 10.1039/D3CP01214J \u003c/li\u003e\n\u003cli\u003eKhan M, Shaik M R, Khan S T, Adil S F, Kuniyil M, Khan M, Al-Warthan A A, Siddiqui M R H, Tahir M N (2020) Enhanced Antimicrobial Activity of Biofunctionalized Zirconia Nanoparticles. ACS Omega 5: 1987\u0026ndash;1996.\u003c/li\u003e\n\u003cli\u003eKhattab E-S R, El Rehim S S A, Hassan W M I, El-Shazly T S (2021) Band Structure Engineering and Optical Properties of Pristine and Doped Monoclinic Zirconia (m-ZrO2): Density Functional Theory Theoretical Prospective. ACS Omega 6: 30061\u0026ndash;30068. https://doi.org/10.1021/acsomega.1c04756\u003c/li\u003e\n\u003cli\u003eKumar H, Bhardwaj K, Kuča K, Kalia A, Nepovimova E, Verma R, Kumar D (2020) Flower-based green synthesis of metallic nanoparticles:applications beyond fragrance. \u003cem\u003eNanomaterials\u003c/em\u003e, \u003cem\u003e10\u003c/em\u003e(4): 766. https://doi.org/10.3390/nano10040766\u003c/li\u003e\n\u003cli\u003eKumari N, Sareen S, Verma M, Sharma S, Sharma A, Sohal H S, Mehta S K, Park J, Mutreja V (2022) Zirconia-based nanomaterials: recent developments in synthesis and applications. Nanoscale Adv. 4(20): 4210\u0026ndash;4236.doi: 10.1039/d2na00367h\u003c/li\u003e\n\u003cli\u003eLastra C J M P, Plou F J, P\u0026eacute;rez-Lebe\u0026ntilde;a E (2021) The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. Int. J. Mol. Sci. 22(9): 4642. https://doi.org/10.3390/ijms22094642\u003c/li\u003e\n\u003cli\u003eMammari N, Lamouroux E, Boudier A, Duval R E (2022) Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles. Microorganisms, 10(2): 437. https://doi.org/10.3390/microorganisms10020437\u003c/li\u003e\n\u003cli\u003eMuthulakshmi N, Kathirvel A, Senthil M, Subramanian (2023) Green synthesis of zirconia nanoparticles and their characterization, anticancer activity and corrosion inhibition properties. R, J. Ind. Chem. Soc., 100 (10): 101076. https://doi.org/10.1016/j.jics.2023.101076\u003c/li\u003e\n\u003cli\u003eNair G M, Sajini T, Mathew B (2022) Advanced green approaches for metal and metal oxide nanoparticles synthesis and their environmental applications. Talanta Open, 2022: 100080. https://doi.org/10.1016/j.talo.2021.100080\u003c/li\u003e\n\u003cli\u003eNarayanan K, Sakthivel C, Inbaraj P (2019) MgO-ZrO2 Mixed Nanocomposites: Fabrication Methods and Applications. Mater. Today Sustain., 3-4(2): 100007. 10.1016/j.mtsust.2019.100007\u003c/li\u003e\n\u003cli\u003eNikolova M P, Chavali M S (2020) Metal Oxide Nanoparticles as Biomedical Materials. Biomimetics, 5(2), 27; https://doi.org/10.3390/biomimetics5020027\u003c/li\u003e\n\u003cli\u003eOhiduzzaman M, Khan M N I, Khan K A, Paul B (2024) Biosynthesis of silver nanoparticles by banana pulp extract: Characterizations, antibacterial activity, and bioelectricity generation. Heliyon, 10: e25520. https://doi.org/10.1016/j.heliyon.2024.e25520\u003c/li\u003e\n\u003cli\u003eOzdal M, Gurkok S (2022) Recent advances in nanoparticles as antibacterial agent. ADMET DMPK. 10(2): 115\u0026ndash;129.doi: 10.5599/admet.1172\u003c/li\u003e\n\u003cli\u003eSagadevan S, Podder J, Das I (2016) Hydrothermal synthesis of zirconium oxide nanoparticles and its Characterization. J Mater Sci: Mater Electron 27:5622\u0026ndash;5627.DOI 10.1007/s10854-016-4469-6\u003c/li\u003e\n\u003cli\u003eSandhya M, Ramasamy D, Sudhakar K, Kadirgama K, Haruna W S W (2021) Ultrasonication an intensifying tool for preparation of stable nanofluids and study the time influence on distinct properties of grapheme nanofluids \u0026ndash; A systematic overview. Ultrason Sonochem. 73: 105479.doi: 10.1016/j.ultsonch.2021.105479\u003c/li\u003e\n\u003cli\u003eShafey A M E (2020) Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: A review. Green Processing and Synthesis, 9: 304-339. https://doi.org/10.1515/gps-2020-0031\u003c/li\u003e\n\u003cli\u003eSharma S (2022) Structure-activity relationship of some pentacoordinated Tributyltin(IV) complexes derived from sterically hindered Schiff bases of heterocyclic \u0026beta;-diketones. J. Ind. Chem. Soc., 99(6): 100464. https://doi.org/10.1016/j.jics.2022.100464\u003c/li\u003e\n\u003cli\u003eSharma S, Kumar P, Jain A, Saxena S (2018) Synergy Between DFT Calculations and Experimental Studies on the Optimized Structures and the Antibacterial Potential of Some Novel Tetra- and Penta Coordinated Organic- Inorganic Hybrid Complexes of Titanium(IV). (2018), Appl. Organomet. Chem., 32(6): e4321. https://doi.org/10.1002/aoc.4321\u003c/li\u003e\n\u003cli\u003eSharma S, Kumar R, P Kumar, Jain A, Saxena S (2019) New insights into the predicament of DFT assisted optimized energy, stability and distortions of optimized topologies of some novel complexes of Zirconium (IV) and enhancement of antimicrobial potential. Appl. Organomet. Chem., 33(10): e5080. https://doi.org/10.1002/aoc.5080\u003c/li\u003e\n\u003cli\u003eShen L, Pang S, Zhong M, Sun Y, Qayum A, Liu Y, Rashid A, Xu B, Liang Q, Ma H, Ren X (2023) A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies. Ultrasonics Sonochemistry, 101: 106646. https://doi.org/10.1016/j.ultsonch.2023.106646\u003c/li\u003e\n\u003cli\u003eSohail M, Baig N, Sher M, Jamil R, Altaf M, Akhtar S, Sharif M (2020) A Novel Tin-Doped Titanium Oxide Nanocomposite for Efficient Photo-Anodic Water Splitting. ACS Omega, 5: 6405\u0026minus;6413. https://dx.doi.org/10.1021/acsomega.9b03876.\u003c/li\u003e\n\u003cli\u003eSzczyglewska P, Feliczak-Guzik A, Nowak I (2023) Nanotechnology\u0026ndash;General Aspects: A Chemical Reduction Approach to the Synthesis of Nanoparticles. Molecules. 28(13): 4932. doi: 10.3390/molecules28134932\u003c/li\u003e\n\u003cli\u003eTalam S, Karumuri S R, Gunnam N (2012) Synthesis, Characterization, and Spectroscopic Properties of ZnO Nanoparticles. ISRN Nanotechnol. 372505. https://doi.org/10.5402/2012/372505\u003c/li\u003e\n\u003cli\u003eTran T V, Nguyen D T C, Kumar P S, Din A T M, Jalil A A, Vo D-V N (2022) Green synthesis of ZrO2 nanoparticles and nanocomposites for biomedical and environmental applications: a review. Environ Chem Lett. 20(2): 1309\u0026ndash;1331.\u003c/li\u003e\n\u003cli\u003eTrieu, N D T, Nguyen, T T N, Nguyen, T T T,\u003csup\u003e \u003c/sup\u003eNguyen, T C D,\u003csup\u003e \u003c/sup\u003e Tran T V (2023) A comparative study on the malachite green dye adsorption of chemically synthesized and green MgFe2O4 nanoparticles using gerbera floral waste extract. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-023-29779-w\u003c/li\u003e\n\u003cli\u003eVerma D, Sharma S, Vashishtha M (2022) Evaluation of optimized molecular structure-antimicrobial and antioxidant efficacy relationship of Schiff Bases. Environ. Sci. Poll. Res. https://doi.org/10.1007/s11356-022-23633-1\u003c/li\u003e\n\u003cli\u003eWidoyanti A A E, Chaikong K, Rangsinth P, Saengratwatchara P, Leung G P-H, Prasansuklab A (2023) Valorization of Nam Wah Banana (Musa paradisiaca L.) Byproducts as a Source of Bioactive Compounds with Antioxidant and Anti-inflammatory Properties: In Vitro and In Silico Studies. Foods, 12(21): 3955; https://doi.org/10.3390/foods12213955\u003c/li\u003e\n\u003cli\u003eYakout S M, Hassan H S (2014) Adsorption Characteristics of Sol Gel-Derived Zirconia for Cesium Ions from Aqueous Solutions. Molecules, 19: 9160-9172. doi:10.3390/molecules19079160\u003c/li\u003e\n\u003cli\u003eYing S, Guan Z, Ofoegbu P C, Club P, Rico C, He F, Hong J (2022) Green synthesis of nanoparticles: Current developments and limitations.Environmental Technology \u0026amp; Innovation, 26: 102336. https://doi.org/10.1016/j.eti.2022.102336\u003c/li\u003e\n\u003cli\u003eYing S, Guan Z, Ofoegbu P C, Clubb P, Rico C, He F, J Hong (2022) Green synthesis of nanoparticles: Current developments and limitations. Environ. Technol. Innov., 26: 102336. https://doi.org/10.1016/j.eti.2022.102336\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Musa paradisiaca, antimicrobial, adsorption capacity, antioxidant, Sn-ZrO2 nanocomposites","lastPublishedDoi":"10.21203/rs.3.rs-4946370/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4946370/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe production of nanoparticles using plant extracts has been the subject of much exploration and study in recent times since it is a cost-effective and environmentally friendly method that reduces the use of hazardous chemicals. In this work, \u003cem\u003eMusa paradisiaca\u003c/em\u003e (banana) peel extract was used to synthesize Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites under ultrasonic irradiation. As a capping and reducing agent in the manufacture of Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites, banana peel extract is crucial. Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites were synthesized in a green manner were effectively evaluated using a FT-IR spectroscopy, UV-Vis spectrophotometer, X-ray diffraction analysis (XRD), energy-dispersive X-ray spectroscopy and scanning electron microscopy (SEM-EDS). Studies have been conducted on the antimicrobial properties of synthesized ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites doped with tin against both Gram positive and Gram negative pathogenic bacteria and fungus. Furthermore, free radical scavenging activity against the DPPH and ABTS assay was used to assess the antioxidant activity of green Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites. The biomimetic synthesised Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites demonstrated robust antioxidant activity and significant antimicrobial activity that was on par with standard. Further, Sn-ZrO\u003csub\u003e2\u003c/sub\u003e nanocomposites shows excellent adsorption capacity of malachite green dye.\u003c/p\u003e","manuscriptTitle":"Green Biogenic Synthesis of Sn-ZrO2 Nanocomposites Using Musa paradisiaca Peel Extract Under Sonication for Biological and Adsorption Efficacy","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-23 10:17:12","doi":"10.21203/rs.3.rs-4946370/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"88f92cb2-6798-4297-a133-a3f5c593527a","owner":[],"postedDate":"September 23rd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-03-12T16:46:59+00:00","versionOfRecord":[],"versionCreatedAt":"2024-09-23 10:17:12","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4946370","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4946370","identity":"rs-4946370","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}