Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate

Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate Cecilia Taulé, Analía Lima, Martín Beracochea, Rosario Durán, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4034332/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 03 Dec, 2024 Read the published version in Plant and Soil → Version 1 posted 6 You are reading this latest preprint version Abstract Backgroun : Plant-associated microbiotas play a key role in plant health, growth, and stresses resilience. These microbiotas are considered the second plant genome by expanding its genetic potential. One of the main components of the plant microbiota is the endophytic bacterial communities, which live in the plant's internal tissues. The main sources of this microbiota are the seed and the soil where the plant grows. Particularly, soil bacteria are attracted by different signals present in plant root exudates, to later colonize the rhizoplane and infect internal tissues. Although the colonization and infection processes of endophytic bacteria are well documented, the molecular bases of the mechanisms involved in the plant-endophyte interaction are still poorly understood. Previously it was shown that strain Kosakonia radicincitans UYSO10 promotes the growth of sugarcane plants and was defined as a true endophyte. Moreover, it was demonstrated that the biological nitrogen fixation process is involved in the plant growth promotion observed. The aim of this work is to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the diazotrophic endophytic strain UYSO10 and sugarcane plants. Methodology : a proteomic approach was conducted in the strain UYSO10 exposed or not to sugarcane exudates. Results showed that in the presence of root exudates, strain UYSO10 senses the environment and adapts its proteome to transport and metabolize different nutrients, and to interact with the host plant. These results deepen the knowledge of the potential mechanisms involved in the early stage of plant-bacteria endophyte interaction. Kosakonia radicincitans sugarcane exudates proteomics Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Introduction The plant holobiont concept has an integrative view on the understanding of the plant phenotype. This holobiont is defined as the assemblage of the plant and all the macro and microorganisms (bacteria, archaea, fungi, viruses, protozoa, and algae), that live on or inside the plant, being the plant phenotype the result of the interaction of all of them and the environment (Rosenberg and ZIlber-Rosenberg 2016). One of the main components of the plant holobiont is its associated microbiota communities, which play a key role in the growth and health status of the plant (Berg et al. 2020). Particularly, the endophytic bacterial microbiota, which refers to those bacteria communities that inhabit the internal tissues of the plant, are involved in key processes such as the modulation of plant secondary metabolism (i.e. by producing indole acetic acid, gibberellin, cytokinins, ACC-deaminase), the improvement of nutrient acquisition (by biological N 2 fixation), and enhancing plant defenses and tolerance to abiotic stresses (Adeleke et al. 2021; Taulé et al. 2021). Studying the mechanisms involved in the interactions between the different components of the endophytic microbiota, as well as with their host, is of fundamental importance to understanding the resulting phenotype of the plant holobiont. Among the main sources of endophytic bacteria are the soil bacteria and the endophytic bacteria associated with the seeds (Taulé et al. 2021). During plant development, different signals are exchanged between the components of these microbiotas, as well as with the host plant, triggering a series of bacterial activities resulting in the establishment of the following endophytic bacterial microbiotas (Hassani et al. 2018; Taulé et al. 2021). Particularly, the colonization of plants by soil bacteria begins when they are attracted to the rhizosphere by chemical signals exuded by the plant (Kandel et al. 2017). Subsequently, the bacteria colonize the surface of the rhizoplane, particularly at the sites of active exudation (emergence of lateral roots or hairs, and root tips); to finally enter the internal tissues (Kandel et al. 2017). Although the processes of colonization and infection of endophyte bacteria are well documented, the molecular bases of the mechanisms involved in the plant-endophyte interaction are still poorly understood. Previously, it was shown that sugarcane cultivars grown in Uruguay can obtain significant amounts of N through the biological nitrogen-fixation (BNF) process. From those N 2 -fixing cultivars, a collection of associated plant growth-promoting bacteria (PGPB) was constructed and characterized (Taulé et al. 2012). Through greenhouse assays, it was shown that strain UYSO10 promotes the growth of micropropagated and cutting sugarcane plants cv. LCP 85-384 (LCP) (Taulé et al. 2016, 2019). In addition, the strain UYSO10 was defined as a diazotroph and as a true endophyte of sugarcane plants (Taulé et al. 2012, 2016). Subsequently, the genome was sequenced, analyzed, and the strain was defined as belonging to the species Kosakonia radicincitans (Beracochea et al. 2019). In silico genome analysis showed the presence of two nitrogenase-encoding operons, Mo- and Fe-nitrogenase (Beracochea et al. 2019). Simple and double mutants in the nitrogenase structural nifH and anfH genes were constructed, and their in vitro characterization showed that both enzymes were active. Moreover, by inoculation of these mutants into sugarcane plants in greenhouse assays, it was demonstrated that the BNF process is involved in the PGP observed (Taulé et al. 2019). This work aims to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the strain Kosakonia radicincitans UYSO10 and sugarcane plants through two orthogonal proteomic approaches. Materials and methods Bacterial strain and plant cultivar Endophytic bacterial strain K. radicincitans UYSO10 was isolated from sugarcane cultivar FAM 81-72 (Taulé et al. 2012). Micropropagated sugarcane plants cv. LCP were produced and supplied by the Biotechnology Unit, Instituto de Investigaciones Agropecuarias (INIA) as previously described (Taulé et al. 2016). In the interaction studies, micropropagated plants were cultivated in 1/10 MS liquid culture medium supplemented with Staba vitamins, and without N source (MS-N) (Reis et al. 1999; Taulé et al. 2016). Bacterial exposure to root exudates The strain UYSO10 was grown in 20 ml of TY culture medium for 24 h. Cells were harvested by centrifugation, the pellet was suspended in MS-N culture medium, and incubated for 16 h at 30 ºC (adapted cells). In parallel, micropropagated sugarcane plants were grown in flasks containing 40 ml of MS-N culture medium. On the 3rd day plants were removed and the culture media was filtered (MSE medium). Adapted cells were inoculated to flasks containing 40 ml of MS-N or MSE media (1.25 x 10 9 cells/ml), and incubated at 30 °C. After 6 h of incubation, cells were harvested by centrifugation for 10 min at 6,000 g at 4 ºC, the pellet was washed and frozen at -20 ºC until use. Four independent biological replicates were used, where a biological replica was considered as the pool of cells obtained from 4 flasks. Protein extraction and quantification Bacterial pellets obtained were suspended in extraction buffer containing 8 M urea, 2 M thiourea, 4% w/v CHAPS, and 10 mM dithiothreitol (DTT), with agitation for 30 min at 4 ºC. Samples were sonicated 3 times for 15 s on ice and again incubated with agitation for 30 min. Finally, samples were centrifuged for 20 min at 20.000 g and 4 °C, and the supernatants were conserved at -20°C for further analysis. Protein concentration was estimated using the Bradford method (Bradford 1976), and protein quality extraction was evaluated by SDS-PAGE (12 % acrylamide). 2D-Difference Gel Electrophoresis (DIGE) For DIGE analysis, 300 µl of 10% trichloroacetic acid (TCA) were added to 200 µg of protein samples and incubated for 15 min on ice with agitation. Subsequently, 300 µl of 0.2% deoxycholate were added to the samples, centrifuged at 15.000 g for 5 min at 4 ºC, suspended in 40 µl of 0.2% deoxycholate and additionally centrifuged for 5 min. Pellets obtained were suspended in 25 µl of H 2 O with vigorous agitation and incubated immediately with 1 ml of pre-cooled acetone overnight at –20 ºC. After that, samples were centrifuged at 15.000 g for 10 min at 4 °C, and the pellets obtained were dry at room temperature and suspended in 40 µl of solubilization buffer (8 M urea, 2 M thiourea, 4 % w/v CHAPS, 30 mM Tris pH 8.5) with agitation for 1 h on ice. Finally, the samples were centrifuged at 20.000 g for 15 min at 4 ºC, and the supernatant was collected. For protein labeling with the CyDye DIGE fluor, the GE Healthcare Kit (GE, Chicago USA) was used. Briefly, 50 µg of each protein sample (bacteria exposed to or not to plant exudates) were labeled with 400 pmol of Cy3 and Cy5 fluor. An internal standard was prepared including 25 µg of each protein sample and labeled with the Cy2 fluor. To compensate for any labeling bias, Cy3 and Cy5 were alternatively used to label the incubated and not incubated protein samples, while Cy2 was exclusively used for labeling the standard. The labeling reaction was carried out for 30 min, in the dark on ice, and stopped by incubation with 10 mM lysine. Equal amounts of sample (from different experimental groups and labeled with different dyes) and the internal standard were mixed and separated in an individual 2-D electrophoresis. For that samples were loaded into 24 cm IPG-strips (pH 3–10 non-linear) on IPGbox (GE Healthcare, Chicago USA), and they were separated by isoelectric focusing on an IPGphor III equipment (GE Healthcare, Chicago USA), employing the recommended voltage profile. Next, disulfide bonds were reduced with 1% w/v dithiothreitol and subsequently alkylated with 4,7% w/v iodoacetamide. The second dimension was performed on a 12 % polyacrylamide SDS-PAGE hand-cast gel, using the Ettan DALT six Electrophoresis System (GE Healthcare), equipped with the Multitemp III cooling unit (GE Healthcare) thermostatted at 20 ºC. The current applied was settled for the first 45 min at 2 W per gel and then increased for 4-5 h to 17 W per gel. Gels were scanned on a Typhoon FLA 9500 scanner (GE Healthcare), using the ImageQuant TL v8.1 software (GE Healthcare) at a resolution of 100 μm, and using the laser wavelength and the filter settings indicated for each dye. The photomultiplier tube voltage was adjusted on each channel to give maximum pixel values below saturation levels (60,000–90,000 counts). Subsequently, the images were exported to the DeCyder 2D Differential Analysis Software 7.2 program (GE Healthcare) for analysis. Then, all the spots of each gel were detected, quantified, and normalized with the internal standard using the Difference In Gel Analysis module. After that, the relative abundance of each spot between the gels was compared by using the Biological Variation Analysis module. Finally, those spots that presented a change rate greater than 1.5 folds and were statistically supported with a Student test (p-value of 0.05), were selected for identification. Protein identification by MALDI-TOF/TOF Gels were fixed for 30 min with ethanol: acetic acid: water (5:1:4) and stained overnight with a solution of Coomassie Blue G-250 in ethanol (8% w/v ammonium sulfate, 0.8% v/v phosphoric acid, 0.08% w/v Coomassie Blue G-250, 20% v/v ethanol). The excess dye was removed by successive washings with distilled water. The spots recognized as differential were located on the gel and carefully excised. The spots were processed as previously reported (Lima et al. 2021), and analyzed by mass spectrometry in a 4800 MALDI-TOF/TOF equipment (Abi Sciex). Mass spectra were calibrated using a mixture of peptides standard (Applied Biosystems) and trypsin autolysis products. Some peptides from all protein spots were selected for MS/MS analyses using the following settings: 8 kV and 15 kV for sources 1 and 2, respectively. The spectra obtained were compared with the proteome of the strain UYSO10, using the MASCOT search server ( http://www.matrixscience.com ) in Sequence Query Mode. The search parameters in Mascot were: i- allowed trypsin cut jumps: 1; ii- variable modifications: methionine oxidation and fixed modification: carbamidomethylation of cysteines; iii- peptide mass tolerance: 0.08 Da and MS/MS tolerance: 0.3-0.5 Da. The m/z values used correspond to the monoisotopic values. The identification of a protein was considered when the Mascot protein score was statistically significant (p < 0.05) and at least one fragmentation was assigned with a significant Mascot peptide ion score (p < 0.05). Nano LC-MS/MS analysis and protein identification Protein samples (15 µg) were loaded onto a pre-made polyacrylamide gel with a 4-12% non-linear gradient (NuPAGE, MES System, Invitrogen), and run for 1 h 15 min under a constant current of 160 V. Next, the gel was fixed and stained with Coomassie Blue G-250 as described above. Each gel lane was cut into 6 fragments, which were first destained with a solution of 50 % acetonitrile (ACN), 0.2 M ammonium bicarbonate (1:1) in ultrapure water for 1 h, with agitation at 30°C. Then, each gel fragment was reduced with 10 mM DTT for 1 h at 56 °C and alkylated with 55 mM iodoacetamide for 45 min at room temperature. Subsequently, the samples were washed with 0.2 M ammonium bicarbonate pH 8, for 15 min and dehydrated by two incubations of 10 min with ACN 100 % under agitation. Finally, the ACN was removed and the gel fragments dried completely with the tube open at room temperature in a laminar flux cabinet. In gel -digestion with trypsin was carried out overnight at 37 °C and peptide extraction was obtained by vigorously incubation (1400 rpm) the samples for 2 h on phase B (60 % ACN, 0.1 % formic acid), at 30 ºC. Peptide samples were vacuum dried, the pellet resuspended with 0.1 % formic acid (phase A), with sonication for 15 s, and centrifuged for 20 min at 16.000 g and 4 ºC. The supernatants were kept as samples. Samples were separated using a nano-HPLC system (EASY-nLC 1000, Thermo Scientific), coupled to an LTQ Velos mass spectrometer (Thermo Scientific). Peptide mixtures were injected into an Acclaim PepMap 100, Nanoviper C18 nano-trap column (75 μm × 2 cm, Thermo Scientific) and separated on a PepMap RSLC C18 (50 μm × 150 mm, Thermo Scientific). Peptide elution was achieved with a flow rate of 250 nl/min and a 100 min gradient from 0 % to 50% of mobile phase B. Peptide analysis was performed in an LTQ Velos nano-ESI linear ion trap equipment (Thermo Scientific) in a data-dependent acquisition mode. Xcalibur 2.1 was used for data acquisition in two steps: 1. acquisition of full MS scan in the positive ion mode with m/z between 300 and 1800 Da, 2. sequential fragmentation of the ten most intense ions, using a dynamic exclusion list (exclusion duration 30 s). A normalized collision energy of 35 and an isolation width of 2 m/z were set. The activation Q was set at 0.25, and the activation time was 15 ms. MS source parameters were set as follows: 2.3 kV electrospray voltage and 260 ◦C capillary temperature. For data analysis, the program PatternLab for Proteomics v4.0 (http://www.patternlabforproteomics.org/) was used (Carvalho et al. 2016). First and using the proteome of the strain UYSO10, a new database of the target reverse type was generated, which included the 127 most common contaminants (e.g. keratin, BSA, among others). Next, the identification of the peptides in the sample was carried out by comparing the data obtained in the mass spectrometer, with the generated database using the Comet Search Engine. In this procedure the following parameters were taken into account: i- precursor mass tolerance: 800 ppm from the measured precursor m/z , ii- enzyme: trypsin with full specificity, accepting a maximum of 2 cuts skipped by the enzyme, iii- fixed modification: carbamidomethylation of cysteines and variable: oxidation of methionine, and iv- at most two variable modifications per peptide. Subsequently, peptide spectrum matches were filtered using the Search Engine Processor (SEPro), and acceptable false discovery rate (FDR) criteria were set as follows: ≤ 3% at the spectra level, ≤ 2% at the peptide level, ≤ 1% at the protein level, and a minimum of two sequences peptides assigned by identified protein. PatternLab’s statistical model for the Approximately Area Proportional Venn Diagram module was used to compare conditions and determine proteins uniquely detected in each experimental condition, using as a criterion that they are present in at least 3 of 4 replicates of the treatment, but in 0 or 1 of the other treatment. PatternLab’s T-Fold module was used to detect proteins present in both conditions at significantly different levels by spectral count analysis and (p-value of 0.05). Proteomic data availability Proteomics data generated in the present work have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD050409. Evaluation of the capacity to grow on different C and N sources The utilization of different carbon sources by the strain UYSO10 was evaluated using the KB009 HiCarbohydrate™ kit (Himedia). Complementary five microliters of strain UYSO10 washed cells, from an overnight LGI medium culture, were inoculated on plates containing LGI medium with C and N substitution. The sources to be tested were added at the final concentrations as per the original protocol (Baldani et al. 1984). The C sources tested were: non-refined sugar, sucrose, glucose, mannitol, lactose, and the organic acids: malate, succinate, fumarate, pyruvate, and acetate. In addition, 100 and 200 g/l of sucrose and non-refined sugar were also tested. The N sources tested were: ammonia, nitrate, L-asparagine, glutamic and aspartic acids, and urea. Additionally, the use of apoplast and root exudates was tested both as C and N sources. The apoplast was obtained by centrifugation, from stem internodes of field sugarcane plants (Dong et al 1994), while root exudates were obtained as described above. All the aforementioned determinations were performed in triplicate. Results Proteome response of strain UYSO10 to sugarcane root exudates analyzed by DIGE The gel images analyzed detected 1950 spots, of which 59 (3% of the total) showed differential relative abundance (34 spots increased, 25 spots decreased in the presence of exudates) (Figure 1 and S1). From this set of differentially expressed proteins, 34 were located in the stained gel and excised for their identification by MALDI-TOF/TOF. From that, 16 were identified and validated by comparing the theoretical mass and the predicted isoelectric point observed experimentally (Table 1). Proteins upregulated in the presence of root exudates were associated with carbohydrate and amino acid metabolism (Table 1). On the other hand, downregulated proteins were related to membrane biogenesis, signal transduction, carbohydrate metabolism, and adaptation to stress. Proteome response of strain UYSO10 to sugarcane root exudates analyzed by nano LC-MS/MS Using a shotgun proteomic approach, a total of 897 proteins were identified, representing 15.1% of the genome CDS. From that, 4 and 59 proteins were exclusively identified in the presence and absence of roots exudate respectively (Table 2). From the total proteins evaluated, 834 were identified in both conditions, where 50 proteins showed differential relative abundance. Among those, in the presence of exudates 14 proteins (0.2 %) were identified as upregulated, while 36 proteins (0.6 %) as downregulated (Figure 2 and Table 3). Taking together unique proteins showing differential relative abundance, we observed that in the presence of exudates, 0.3 % of the total proteome was up-regulated, while 1.6 % was down-regulated (Figure S1). The cell localization of the differential expressed proteins was achieved using PSORTB and Cello tools. Proteins were classified into cytoplasmic (55), cytoplasmic membrane (19), periplasmic (15), outer membrane (7), and extracellular (3) (Tables 2 and 3). Concerning COG functional categories, differentially expressed proteins were mainly associated with carbohydrate and amino acid transport and metabolism, biogenesis and membrane structure, and energy production and conversion (Tables 2 and 3, Figure S2). Strain UYSO10's ability to use different C and N sources Results indicate that strain UYSO10 could utilize a great variety of C sources including sucrose, non-refined sugar, trehalose, glucose, fructose, mannitol, xylose, maltose, dextrose, L-arabinose, mannose, sorbose, acetate, citrate, malate, pyruvate, succinate, fumarate and salicin (Table S1). Concerning the ability to use different N-sources, results showed that strain UYSO10 can use all the tested compounds (Table S1). Finally, results also showed that the strain UYSO10 can use either the sugarcane root exudates, as well as the apoplast as a sole C and N-sources (Table S1). Discussion Roots exudate in the establishment of the plant-bacteria interaction Plants exudate between 20-40 % of their photosynthetic products to the soil, which consists mainly of complex sugars, amino acids, organic acids, extracellular enzymes, phenols, vitamins, and nitrogenous macromolecules (Haichar et al. 2014; Canarini et al. 2019; Vives-Peris et al. 2020). Compounds present in root exudates serve as a source of C and N for soil bacteria, shaping the rhizospheric community's structure, diversity, and functionality (Haichar et al. 2014). Particularly, some exudates compounds serve also as signals including sugars, amino acids, carboxylic acids (succinate, fumarate, malate), and aromatic compounds (luteolin, catechols, acetosyringone, among others), which induce a flagella-dependent chemotaxis in soil bacteria towards the roots (Haichar et al. 2014). The main points of root exudates release are the root hairs, the apical zone, as well as the emergence sites of secondary roots, being the preferred colonization sites for the rhizospheric and endophytic bacteria (Haichar et al. 2014; Canarini et al. 2019; Compant et al. 2021). Diverse physical, chemical, and biological environmental factors such as the presence of biotic and abiotic stress, as well as the structure of the soil, modulate quantitatively and qualitatively the composition of the exudate (Canarini et al. 2019; Vives-Peris et al. 2020). At the same time, the exudation of certain specific molecules depends in part on the recognition of molecular signals secreted by soil bacteria in response to signals previously secreted by the plants (Mark et al. 2005; Witzel et al. 2017). In this sense, it is considered that the molecular signaling between bacteria and plants plays a fundamental role in establishing beneficial and pathogenic interactions. However, the molecular mechanisms underlying plant-endophytic bacterial interactions are poorly understood (Haichar et al. 2014; Coskun et al. 2017). Previously, it was demonstrated that the strain UYSO10 mainly colonized the emergence zone of lateral roots of sugarcane plants (Taulé et al. 2016). Therefore in this work, the bacterial response to sugarcane root exudates was evaluated in the same culture medium (Taulé et al. 2016). The MS-N culture medium employed has a low C source concentration (sucrose 2 g/l), and does not have an N-source which favors the interaction with diazotrophic bacteria (Reis et al. 1999; Lery et al. 2011; Taulé et al. 2016). But this low nutrient condition also constitutes a stress situation for both plants and bacteria and particularly defines a specific plant exudation profile. For instance, when N-nutrients are poorly available, plants stimulate lateral root proliferation, and reduce amino acid exudation, while increasing organic acid exudation (Canarini et al. 2019; Vives-Peris et al. 2020). Proteomic response of the endophytic bacteria Kosakonia radicincitans UYSO10 to root exudates In the present work, two proteomic strategies were used to address the strain UYSO10 response to sugarcane root exudates. Results obtained showed that all the proteins identified by applying the DIGE strategy were identified using nano LC-MS/MS. However, not all proteins showed the same expression trend in both approaches (Table S2). These differences may be associated with the nature of the techniques employed. The DIGE technique separates proteins and their proteoforms, and eventually being able to identify post-translational modifications; however, this technique is not sensitive to low-concentration proteins. On the other hand, by using the nano LC-MS/MS technique, the total populations of each protein (all the proteoforms), are quantified and it is sensitive to identify proteins expressed in lower concentrations. The following analysis integrates the results obtained by both proteomics approaches used in this work. Nutrient transport and metabolism As strain UYSO10 is an endophytic bacterium, it was expected that in the first steps of the interaction with the plants, it would be able to capture and metabolize the compounds exuded by the plant either as a nutritional source or as a signal. C metabolism. Strain UYSO10 constitutively expressed proteins associated with the transport and metabolization of different C-sources (Figure 3). Interestingly, in the presence of root exudates, no evidence of a specific C-source use was detected since no upregulation of any transporter was identified under the conditions evaluated. On the other hand, in the absence of exudates, proteins associated with the transport and metabolization of different C-sources were up-regulated, including fructose and acetate (Figure 3). The main C-source in the MS-N medium is sucrose and it seems that the monomer glucose is used in both conditions evaluated, while fructose is also used in the absence of root exudate. It should be noted that the strain UYSO10 can grow using sucrose, glucose, and fructose as the sole C-source (Table S1). Interestingly, the same behavior of differential use of sucrose monosaccharides has been reported in the strain E. coli W grown under low concentrations of sucrose (Sabri et al. 2013). Thus, it seems that in the absence of root exudates the strain UYSO10 expresses a battery of C-transporters to take any potential C-source from the growth medium. While, in the presence of root exudate the strain UYSO10 uptake the C-sources using the constitutively expressed C-transporters. In the presence of root exudates, some enzymes of the Krebs cycle and energy metabolism (e.g. F1F0-ATPase) were up-regulated, suggesting the bacteria is metabolically active (Figure 3). Particularly, in similar work, the succinyl-CoA ligase enzyme was also identified as up-regulated in the strain Gluconacetobcter diazotrophicus Pal5 growing in co-culture with sugarcane plants (Dos Santos et al. 2010). This expression was correlated with the production of EPS and certain succinic-glycans, indicating a possible role in the establishment of bacteria-plant associations (Dos Santos et al. 2010). N metabolism . Although the strain UYSO10 is diazotrophic, and the genome encodes for two nitrogenases (Beracochea et al. 2019), the enzymes were not detected, probably due to the presence of oxygen in both conditions evaluated (de Bruijn 2015). Interestingly, the down-regulation of specific proteins related to the uptake of different N-sources was observed in the presence of root exudates. This correlates with a shift in the N-sources disponibility, from an N-limiting to an N-no-limiting situation due to the presence of easily available and assimilable N-compounds present in the roots exudates (Zimmer et al. 2000). Particularly, it seems that one of the N sources used in the presence of root exudates is aspartate (Figure 4, Tables 1 and 3). This correlates with the fact that aminoacids are the main N-source in the sugarcane apoplast, being the aspartic acid the fourth most abundant (Tejera et al. 2006); as well as the availability of the strain UYSO10 to use aspartate as a sole N-source (Table S1). Osmotic stress. The proteomic analysis showed the constitutive expression of the gluconeogenesis pathway in both conditions evaluated and the up-regulation of two of its enzymes in the presence of root exudates (Figure 3). This observation, together with the repression of the synthesis of structural components such as glycogen, cellulose, and their derivatives, suggests that the strain UYSO10 may be synthesizing trehalose in the presence of root exudates (Figure 3). Since the synthesis and storage of trehalose was described as associated with osmotic stress, it is likely that the strain UYSO10 detects the presence of the plant from the exudates components and prepares its metabolism for future osmotic changes during the interaction (Ormeño-Orrillo et al. 2012). It is important to emphasize that strain UYSO10 was isolated from plates containing LGI-P culture media (LGI with 100 g/l of sucrose) and can grow on LGI medium containing 200 g/l of sucrose, as well as non-refined sucrose. From these results, strain UYSO10 could be considered an osmotolerant bacteria. In addition, a subunit of the first enzyme in the biosynthesis pathway of fatty acids (acetyl-CoA-carboxylase), was also identified as up-regulated in the presence of root exudates (Figure 3). Gluconacetobacter diazotrhophicus Pal5 mutants in another subunit of this enzyme are affected at high osmotic environments, highlighting its role in the adaptation to osmotic stress (Leandro et al. 2021). Moreover, fatty acids and modified fatty acids were described as important molecules during plant colonization by microorganisms (Pohl and Kock 2014; Uranga et al. 2016). More studies are necessary in the strain UYSO10 to understand the role of fatty acids during the plant-bacteria interaction. Mobility and chemotaxis. The bacterial transition from a mobile state to biofilm formation requires the expression of transcriptional regulators, which respond to environmental signals such as root exudates (Han et al. 2023; Liu et al. 2024). In this sense in the presence of root exudates, the strain UYSO10 up-regulated the universal stress protein G, as well as the stringent response regulator DksA and the CheZ protein (Figure 5). Those proteins are associated in bacteria with an increase in the formation of aggregates and a decrease in chemotaxis and motility (Nachin et al. 2005; Min and Yoon 2020). Moreover, under the conditions mentioned, the structural protein of the flagellum FliF and four methyl-accepting chemotaxis proteins (MCP) were identified as down-regulated. The repression of genes linked to flagellum synthesis in plant-associated bacteria has been postulated as a strategy to evade plant defenses, since some of them like flagellin, were described as MAMP (Mark et al. 2005; Cheng et al. 2009; Shidore et al. 2012; Paungfoo-Lonhienne et al. 2016). However, the inhibition of the flagellum is not universal, for instance, in H. seropedicae SmR1 growing in the presence of sugarcane extracts, the up-regulation of flagellin was reported, and associated with plant colonization and the formation of biofilms (Cordeiro et al. 2013). Probably, a fine-tuning of the flagellar apparatus during the endophytic bacterial colonization takes place intending to avoid the plant defense and allow root colonization. Finally, the up-regulation of the aerotaxis sensory receptor (AerC) in the presence of root exudates, together with the low concentration of N-sources in the culture medium, could indicate that the strain UYSO10 is searching for a microaerophilic condition that allows the BNF process, as was reported in other diazotrophs (Yao and Allen 2007; Xie et al. 2010). Bacterial cell envelope Membrane proteins play an important role in processes such as signal transduction, and transport, as well as in cell-cell interaction, among others. The results obtained showed that in the presence of root exudates, the strain UYSO10 presented large changes in the expression of proteins associated with the biogenesis and structure of the cell envelope, suggesting a strong reorganization and therefore adaptation (Figure 5). Probably, some of these changes are associated with a potential compatible interaction with the plant perceived by the bacteria (Balsanelli et al. 2016; Pankievicz et al. 2016). For example, OmpF and OmpH proteins were up-regulated in the presence of root exudates. Both proteins were previously reported as up-regulated in Pseudomonas putida and G. diazotrophicus in the presence of root exudates and co-culture with sugarcane respectively. The proposed role for those proteins includes the adhesion to the plant cell wall, the transport of small molecules like ACC, as well as receptors, and chaperon, beyond others (Cheng et al. 2009; Lery et al. 2011). On the other hand, several membrane structural proteins (OmpC, OmpA, SecE, and TolB), and membrane biogenesis proteins (YaeT, MsbA, LpoA), as well as proteins associated with the synthesis and transport of LPS, were down-regulated in the presence of root exudates. Many of them have been described as immunogenic antigens, so their down-regulation could be linked to preventing the activation of the plant's immune system (Boller and Felix 2009). Chaperones and stress-related functions Plant-bacteria interaction and nutrient starvation could be considered stressful situations for the bacteria. Therefore in the context of the experimental conditions evaluated, the identification of proteins related to chaperones and stress was expected. In this sense, in the presence of root exudates was identified as up-regulated the stringent response regulator DskA and the chaperone Heat shock GroES protein (Figure 5). The versatile role of the DskA regulator including its role in the virulence of the strain Pseudomonas aeruginosa was described (Min and Yoon 2020). The chaperons GroES and GroEL were identified as up-regulated in the strain G. diazotrophicus , in co-culture with sugarcane plants (Dos Santos et al. 2010; Lery et al. 2011). In addition, the expression of the GroEL protein in Sinorhizobium meliloti has been associated with the regulation of the nod genes (Ogawa and Long 1995). Together these reports suggest that during the strain UYSO10-sugarcane interaction, the GroES chaperones could be involved in a specific adaptation and survival response. Finally and in the presence of root exudates, was identified as up-regulated the Alkyl hydroperoxide reductase protein C (AhpC) (Figure 5), an oxidoreductase protein described as involved in cellular and redox homeostasis (Mongkolsuk et al. 2000). The up-regulation of this enzyme was also identified in the endophytic strain Burkholderia phytofirmans growing endophytically in potato plants (Sheibani-Tezerji et al. 2015). Moreover, the expression of several antioxidant enzymes has been observed in endophytic strains grown in co-culture with their host plants (Dos Santos et al. 2010; Lery et al. 2011; Alquéres et al. 2013). Together these reports suggest a probable role of the protein AhpC in the antioxidant response during the interaction with sugarcane plants. Conclusions Exposure of strain UYSO10 to sugarcane root exudates induces quantifiable changes in the bacterial proteome. Those changes include the remodeling of several pathways toward the metabolic adaptation to the nutrients present in the root exudates. the change from a planktonic lifestyle to the formation of bacterial aggregates, as well as changes in the bacterial membrane. Together are correlated with the necessary steps for bacterial adaptation to the rhizosphere conditions toward rhizoplane colonization and root infection. Declarations Funding This work was supported by grants from the Uruguayan Fund for the Promotion of Agricultural Technology (Fondo de Promoción de Tecnología Agropecuaria FPTA-275 and 331-INIA), the Uruguayan Program for the Development of the Basic Sciences (Programa de Desarrollo de las Ciencias Básicas-PEDECIBA), the Posgrade Academic Commission-UdelaR (Comisión Académica de Posgrado), and the Uruguayan National Agency for Innovation and Research (Agencia Nacional de Innovación e Investigación-ANII). The authors are very grateful to Ing. Agr. Fernando Hackembruch from the Agriculture Department of the Alcoholes Uruguay S.A., and Alicia Castillo from the Biotechnology Unit, Estación Experimental Wilson Ferreira Aldunate, Instituto de Investigaciones Agropecuarias (INIA), Uruguay. Competing Interests The authors have no relevant financial or non-financial interests to disclose References Adeleke BS, Babalola OO, Glick BR (2021) Plant growth-promoting root-colonizing bacterial endophytes. Rhizosphere 20:100433. https://doi.org/10.1016/j.rhisph.2021.100433 Alquéres S, Meneses C, Rouws L, et al (2013) The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by Gluconacetobacter diazotrophicus PAL5. Mol Plant Microbe Interact 26:937–45. https://doi.org/10.1094/MPMI-12-12-0286-R Baldani JI, Baldani VLD, Sampaio MJAM, Doberainer J (1984) A fourth Azospirillum species from cereal roots. An Acad Bras Cienc 56:365 Balsanelli E, Tadra-Sfeir MZ, Faoro H, et al (2016) Molecular adaptations of Herbaspirillum seropedicae during colonization of the maize rhizosphere. Environ Microbiol 18:2343–2356. https://doi.org/10.1111/1462-2920.12887 Beracochea M, Taulé C, Battistoni F (2019) Draft Genome Sequence of Kosakonia radicincitans UYSO10, an Endophytic Plant Growth-Promoting Bacterium of Sugarcane ( Saccharum officinarum . Microbiol Resour Announc 8:e01000-19. https://doi.org/10.1128/MRA.01000-19 Berg G, Rybakova D, Fischer D, et al (2020) Microbiome definition re-visited: old concepts and new challenges. Microbiome 8:1–22. https://doi.org/10.1186/s40168-020-00875-0 Boller T, Felix G (2009) A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors. Annu Rev Plant Biol 60:379–406. https://doi.org/10.1146/annurev.arplant.57.032905.105346 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3 Canarini A, Kaiser C, Merchant A, et al (2019) Root exudation of primary metabolites: Mechanisms and their roles in plant responses to environmental stimuli. Front Plant Sci 10:157. https://doi.org/10.3389/fpls.2019.00157 Carvalho PC, Lima DB, Leprevost, Felipe V Santos, Marlon D.M. Fischer, Juliana S.G. Aquino PF, et al (2016) Integrated analysis of shotgun proteomic data with PatternLab for proteomics 4.0. Nat Protoc 11:102–117 Cheng Z, Duan J, Hao Y, et al (2009) Identification of Bacterial Proteins Mediating the Interactions Between Pseudomonas putida UW4 and Brassica napus (Canola). Mol Plant-Microbe Interact 22:686–694. https://doi.org/10.1094/MPMI-22-6-0686 Compant S, Cambon MC, Vacher C, et al (2021) The plant endosphere world – bacterial life within plants. Environ Microbiol 23:1812–1829. https://doi.org/10.1111/1462-2920.15240 Cordeiro FA, Tadra-Sfeir MZ, Huergo LF, et al (2013) Proteomic analysis of Herbaspirillum seropedicae cultivated in the presence of sugar cane extract. J Proteome Res 12:1142–1150. https://doi.org/10.1021/pr300746j Coskun D, Britto DT, Shi W, Kronzucker HJ (2017) How plant root exudates shape the nitrogen cycle. Trends Plant Sci 22:661–673. https://doi.org/10.1016/j.tplants.2017.05.004 de Bruijn FJ (2015) Biological nitrogen fixation. In: Lugtenberg B (ed) Principles of Plant-Microbe Interactions. Microbes for sustainable agriculture. Springer, pp 215–224 Dos Santos MF, Muniz de Padua VL, Nogueira EDM, et al (2010) Proteome of Gluconacetobacter diazotrophicus co-cultivated with sugarcane plantlets. J Proteomics 73:917–931. https://doi.org/https://doi.org/10.1016/j.jprot.2009.12.005 Haichar F el Z, Santaella C, Heulin T, Achouak W (2014) Root exudates mediated interactions belowground. Soil Biol Biochem 77:69–80. https://doi.org/10.1016/J.SOILBIO.2014.06.017 Han L, Zhang H, Bai X, Jiang B (2023) The peanut root exudate increases the transport and metabolism of nutrients and enhances the plant growth-promoting effects of Burkholderia pyrrocinia strain P10. BMC Microbiol 23:1–16. https://doi.org/10.1186/s12866-023-02818-9 Hassani MA, Durán P, Hacquard S (2018) Microbial interactions within the plant holobiont. Microbiome 6:58. https://doi.org/10.1186/s40168-018-0445-0 Kandel S, Joubert P, Doty S (2017) Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms 5:77. https://doi.org/10.3390/microorganisms5040077 Leandro M, Andrade L, Vespoli L, et al (2021) Comparative proteomics reveals essential mechanisms for osmotolerance in Gluconacetobacter diazotrophicus . Res Microbiol 172:103785. https://doi.org/10.1016/j.resmic.2020.09.005 Lery LMS, Hemerly AS, Nogueira EM, et al (2011) Quantitative proteomic analysis of the interaction between the endophytic plant-growth-promoting bacterium Gluconacetobacter diazotro phicus and sugarcane. Mol plant-microbe Interact 24:562–576 Lima A, Leyva A, Rivera B, et al (2021) Proteome remodeling in the Mycobacterium tuberculosis PknG knockout: molecular evidence for the role of this kinase in cell envelope biogenesis and hypoxia response. J Proteomics 244:104276. https://doi.org/https://doi.org/10.1016/j.jprot.2021.104276 Liu Y, Xu Z, Chen L, et al (2024) Root colonization by beneficial rhizobacteria. FEMS Microbiol Rev 48:1–20. https://doi.org/10.1093/femsre/fuad066 Mark GL, Dow JM, Kiely PD, et al (2005) Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. Proc Natl Acad Sci 102:17454–17459. https://doi.org/10.1073/pnas.0506407102 Min KB, Yoon SS (2020) Transcriptome analysis reveals that the RNA polymerase-binding protein DksA1 has pleiotropic functions in Pseudomonas aeruginosa . J Biol Chem 295:3851–3864. https://doi.org/10.1074/jbc.RA119.011692 Mongkolsuk S, Whangsuk W, Vattanaviboon P, et al (2000) A Xanthomonas alkyl hydroperoxide reductase subunit C ( ahpC ) mutant showed an altered peroxide stress response and complex regulation of the compensatory response of peroxide detoxification enzymes. J Bacteriol 182:6845–6849. https://doi.org/10.1128/JB.182.23.6845-6849.2000 Nachin L, Nannmark U, Nyström T, Nystro T (2005) Differential roles of the universal stress proteins of Escherichia coli in oxidative stress resistance, adhesion, and Motility. J Bacteriol 187:6265–6272. https://doi.org/10.1128/JB.187.18.6265 Ogawa J, Long SR (1995) The Rhizobium meliloti groELc locus is required for regulation of early nod genes by the transcription activator NodD. Genes Dev 9:714–729. https://doi.org/10.1101/gad.9.6.714 Ormeño-Orrillo E, Menna P, Almeida LGP, et al (2012) Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean ( Phaseolus vulgaris L.). BMC Genomics 13:735. https://doi.org/10.1186/1471-2164-13-735 Pankievicz VCS, Camilios-Neto D, Bonato P, et al (2016) RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat ( Triticum aestivum ) roots. Plant Mol Biol 90:589–603. https://doi.org/10.1007/s11103-016-0430-6 Paungfoo-Lonhienne C, Lonhienne TGA, Yeoh YK, et al (2016) Crosstalk between sugarcane and a plant-growth promoting Burkholderia species. Sci Rep 6:37389. DOI: 10.1038/srep37389 Pohl CH, Kock JLF (2014) Oxidized fatty acids as inter-kingdom signaling molecules. Molecules 19:1273–1285. https://doi.org/10.3390/molecules19011273 Reis VM, Olivares FL, de Oliveira ALM, et al (1999) Technical approaches to inoculate micropropagated sugar cane plants were Acetobacter diazotrophicus . Plant Soil 206:205–211. https://doi.org/10.1023/A:1004436611397 Rosenberg E, ZIlber-Rosenberg I (2016) Microbes Drive Evolution of Animals and Plants: the Hologenome Concept. MBio 7:e01395. https://doi.org/doi: 10.1128/mBio.01395-15 Sabri S, Nielsen LK, Vickers CE (2013) Molecular control of sucrose utilization in Escherichia coli W, an efficient sucrose-utilizing strain. Appl Environ Microbiol 79:478–487. https://doi.org/10.1128/AEM.02544-12 Sheibani-Tezerji R, Rattei T, Sessitsch A, et al (2015) Transcriptome profiling of the endophyte Burkholderia phytofirmans psjn indicates sensing of the plant environment and drought stress. MBio 6:e00621-15. https://doi.org/10.1128/mBio.00621-15 Shidore T, Dinse T, Ohrlein J, et al (2012) Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol 14:2775–2787. https://doi.org/10.1111/j.1462-2920.2012.02777.x Taulé C, Castillo A, Villar S, et al (2016) Endophytic colonization of sugarcane ( Saccharum officinarum ) by the novel diazotrophs Shinella sp. UYSO24 and Enterobacter sp. UYSO10. Plant Soil 403:403–418. https://doi.org/https://doi.org/10.1007/s11104-016-2813-5 Taulé C, Luizzi H, Beracochea M, et al (2019) The Mo- and Fe-nitrogenases of the endophyte Kosakonia sp. UYSO10 are necessary for growth promotion of sugarcane. Ann Microbiol 69:741–750. https://doi.org/https://doi.org/10.1007/s13213-019-01466-7 Taulé C, Mareque C, Barlocco C, et al (2012) The contribution of nitrogen fixation to sugarcane ( Saccharum officinarum L.), and the identification and characterization of part of the associated diazotrophic bacterial community. Plant Soil 356:35–49. https://doi.org/10.1007/s11104-011-1023-4 Taulé C, Vaz-Jauri P, Battistoni F (2021) Insights into the early stages of plant–endophytic bacteria interaction. World J Microbiol Biotechnol 37:1–9. https://doi.org/10.1007/s11274-020-02966-4 Tejera N, Ortega E, Rodes R, Lluch C (2006) Nitrogen compounds in the apoplastic sap of sugarcane stem: some implications in the association with endophytes. J Plant Physiol 163:80–85. https://doi.org/10.1016/j.jplph.2005.03.010 Uranga C, Beld J, Mrse A, et al (2016) Fatty acid esters produced by Lasiodiplodia theobromae function as growth regulators in tobacco seedlings. Biochem Biophys Res Commun 472:339–345. https://doi.org/10.1016/j.bbrc.2016.02.104 Vives-Peris V, de Ollas C, Gómez-Cadenas A, Pérez-Clemente RM (2020) Root exudates: from plant to rhizosphere and beyond. Plant Cell Rep 39:3–17. https://doi.org/10.1007/s00299-019-02447-5 Witzel K, Strehmel N, Baldermann S, et al (2017) Arabidopsis thaliana root and root exudate metabolism is altered by the growth-promoting bacterium Kosakonia radicincitans DSM 16656T. Plant Soil 419:557–573. https://doi.org/10.1007/s11104-017-3371-1 Xie Z, Ulrich LE, Zhulin IB, Alexandre G (2010) PAS domain containing chemoreceptor couples dynamic changes in metabolism with chemotaxis. PNAS 107:2235–2240. https://doi.org/https://doi.org/10.1073/pnas.0910055107 Yao J, Allen C (2007) The plant pathogen Ralstonia solanacearum needs aerotaxis for normal biofilm formation and interactions with its tomato host. J Bacteriol 189:6415–6424. https://doi.org/10.1128/JB.00398-07 Zimmer DP, Soupene E, Lee HL, et al (2000) Nitrogen regulatory protein C-controlled genes of Escherichia coli : scavenging as a defense against nitrogen limitation. Proc Natl Acad Sci U S A 97:14674–14679. https://doi.org/10.1073/pnas.97.26.14674 Tables Table 1. Identity and general features of the spots with differential expression detected by DIGE, in the proteome of the strain UYSO10 grown in the presence of sugarcane root exudates. Fold change p value Encoding gene in UYSO10 Mascot score a Sequence coverage Number of peptide asigned Mass (kDa) PI Identified protein b COG category c Celular localization d Up-regulated in the presence of root exudates 2.51 0.01 UYSO10_0935 89 14 % 6 52.2 5.06 Aspartate ammonia-lyase (EC 4.3.1.1) E C 2.21 0.00037 UYSO10_3581 243 17 % 5 46.3 6.28 Urea ABC transporter, substrate binding protein UrtA E P 1.55 0.012 UYSO10_3581 233 24 % 9 46.3 6.28 Urea ABC transporter, substrate binding protein UrtA E P 1.45 0.045 UYSO10_3581 102 11 % 4 46.3 6.28 Urea ABC transporter, substrate binding protein UrtA E P 1.45 0.04 UYSO10_0469 65 21 % 7 41.1 5.08 Phosphoglycerate kinase (EC 2.7.2.3) G C 1.89 0.022 UYSO10_3373 324 47 % 11 25.0 4.27 Uncharacterized protein conserved in bacteria S E 1.7 0.028 UYSO10_2573 91 14 % 4 45,9 7.08 Glucose-1-phosphatase (EC 3.1.3.10) S P 1.55 0.042 UYSO10_2573 75 9 % 3 45.9 7.08 Glucose-1-phosphatase (EC 3.1.3.10) S P Down-regulated in the presence of root exudates 1.69 0.0063 UYSO10_0266 171 24 % 7 32.7 8.99 Maltose operon periplasmic protein MalM G P 1.3 0.016 UYSO10_5888 454 65 % 22 30.8 6.58 Ribose ABC transporter system, periplasmic ribose-binding protein Rbs (TC 3.A.1.2.1) G P 1.77 0.0079 UYSO10_2505 47 7 % 1 24.6 4.35 FIG00955836: hypothetical protein J E 1.58 0.0075 UYSO10_2413 277 41 % 14 37.3 6.12 Outer membrane protein A precursor M OM 1.37 0.025 UYSO10_2413 549 54 % 16 37.3 6.12 Outer membrane protein A precursor M OM 1.37 0.039 UYSO10_0940 164 25 % 12 57.3 4.85 Heat shock protein 60 family chaperone GroEL O C 1.37 0.039 UYSO10_1105 84 22 % 5 23.2 5.06 FIG00975563: hypothetical protein S P* 1.49 0.019 UYSO10_3704 96 14 % 8 63.4 5.78 Trehalase (EC 3.2.1.28) T P a All Mascot scores reported were statistically significant (p <0.05). b The annotation of the proteins was obtained using the genome of the strain UYSO10. c COG categories: J- translation; O- chaperone molecules and related functions; M- structure and biogenesis of the outer membrane; T- signal transduction; G- carbohydrate transport and metabolism; E- transport and metabolism of amino acids; S- no functional prediction. d The results showed correspond to prediction using PSORTb algorithm, and when no output was obtained, Cello algorithm was applied and the result reported is indicated with a "*". OM- Outer membrane, C- Cytoplasmic, P- Periplasmic, E- Extracellular. Table 2. Proteins exclusively identified in the samples of the strain UYSO10 exposed to sugarcane root exudates, detected by the shotgun approach. Gene in UYSO10 Identified protein a COG category b # replicates Spectrum counts c Localization Unique in the presence of root exudates UYSO10_0953 Fumarate reductase iron-sulfur protein (EC 1.3.5.4) C 3 24 MC/P* UYSO10_1506 RNA polymerase-binding transcription factor DksA E 3 25 C UYSO10_0266 Maltose operon periplasmic protein MalM G 4 22 P UYSO10_3373 Uncharacterized protein conserved in bacteria S 4 23 E Unique in the absence of root exudates UYSO10_5355 Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16) C 3 48 MC/C* UYSO10_2947 Fumarate hydratase class II (EC 4.2.1.2) C 4 35 C UYSO10_4156 D-lactate dehydrogenase (EC 1.1.1.28) C 3 27 MC/C* UYSO10_4140 Bifunctional protein: zinc-containing alcohol dehydrogenase C 3 20 C UYSO10_4591 Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-) C 4 18 MC UYSO10_3055 Methionine synthase II (cobalamin-independent) E 3 41 C UYSO10_5669 Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2) E 4 24 MC/C* UYSO10_1401 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8) E 4 21 C UYSO10_0105 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) E 3 19 C UYSO10_0251 Aspartokinase (EC 2.7.2.4) E 3 19 C UYSO10_5671 Dipeptide transport system permease protein DppB (TC 3.A.1.5.2) E 3 17 MC UYSO10_2314 Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2) E 3 16 MC UYSO10_2235 Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4) E 3 16 MC UYSO10_0233 IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) F 3 31 C UYSO10_1623 Xanthine-guanine phosphoribosyltransferase (EC 2.4.2.22) F 3 25 C UYSO10_4191 1-phosphofructokinase (EC 2.7.1.56) G 4 82 C UYSO10_5520 Limit dextrin alpha-1,6-maltotetraose-hydrolase (EC 3.2.1.196) G 4 60 C UYSO10_1103 Dihydroxyacetone kinase, ATP-dependent (EC 2.7.1.29) G 4 45 C* UYSO10_0357 Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1) G 3 20 MC UYSO10_0834 Fructokinase (EC 2.7.1.4) G 3 17 MC/C* UYSO10_1616 Acyl-CoA dehydrogenase I 4 45 MC UYSO10_5362 Ribonuclease G J 3 29 C UYSO10_0499 Peptide chain release factor 2 J 3 19 C UYSO10_3460 TsaC/YrdC paralog J 3 16 C UYSO10_1783 ROK family sugar kinase or transcriptional regulator K 3 31 C UYSO10_5275 Penicillin-binding protein activator LpoA L 3 22 I UYSO10_0281 Replicative DNA helicase (DnaB) (EC 3.6.4.12) L 3 16 C UYSO10_0226 NADH pyrophosphatase (EC 3.6.1.22) L 3 9 C UYSO10_2683 Transcription-repair coupling factor L 3 29 C UYSO10_3519 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55) M 3 31 C UYSO10_4804 Translation elongation factor LepA M 4 21 MC/C* UYSO10_4044 Protein YeeZ M 3 21 C UYSO10_4627 Outer membrane porin for chitooligosaccharides ChiP M 3 20 ME* UYSO10_0778 Membrane-bound lytic murein transglycosylase B M 3 20 MC/P* UYSO10_2237 Apolipoprotein N-acyltransferase (EC 2.3.1.-) M 3 17 MC UYSO10_1030 Methyl-accepting chemotaxis sensor/transducer protein N 3 72 MC UYSO10_3724 TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA O 3 13 C* UYSO10_5397 Trk potassium uptake system protein TrkA P 3 31 MC/C* UYSO10_0721 Nitrate ABC transporter, permease protein P 3 30 MC UYSO10_1733 Periplasmic hemin-binding protein P 3 13 MC UYSO10_2405 Bis-ABC ATPase Uup R 4 36 C UYSO10_5852 Chromate reductase (EC 1.6.5.2) R 3 36 C UYSO10_2150 5-methylthioribose kinase (EC 2.7.1.100) R 3 25 C UYSO10_0341 Acetate permease ActP (cation/acetate symporter) R 4 24 MC UYSO10_3617 Hydrolase, alpha/beta fold family R 4 22 P* UYSO10_5883 ATPase RavA R 3 16 C UYSO10_1220 Multimeric flavodoxin WrbA R 3 14 E UYSO10_0640 Hypothetical protein S 3 50 E UYSO10_5767 Uncharacterized protein YicH S 3 31 P UYSO10_3147 hypothetical protein S 3 18 MC/ME* UYSO10_5622 hypothetical protein S 3 17 P UYSO10_4435 FIG00626109: hypothetical protein S 3 16 P UYSO10_2446 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97) S 3 14 I UYSO10_5361 Uncharacterized protein YhdP S 3 13 I UYSO10_0239 Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-) T 4 22 C UYSO10_0208 Protein translocase subunit SecE U 4 25 MC UYSO10_3136 Beta-lactamase (EC 3.5.2.6) V 4 21 P/ME* UYSO10_2773 hypothetical protein V 3 19 C UYSO10_2374 Lipid A export permease/ATP-binding protein MsbA V 3 15 MC a The protein annotation is obtained from the search using the strain UYSO10 genome database. b COG categories: J- translation; L- replication, recombination and repair; K- transcription; O- chaperone molecules and related functions; M- structure and biogenesis of the cell wall and the outer membrane; N- secretion, mobility and chemotaxis; T-signal transduction; P-transport and metabolism of inorganic ions; C-energy production and conversion; G-transport and carbohydrate metabolism; E-transport and metabolism of amino acids; F-transport and nucleotide metabolism; U- intracellular traffic and secretion; V- defense mechanism; I-lipid metabolism; R-general functional prediction; S-no functional prediction. c The total signal corresponds to the sum of the number of spectra assigned to each protein in each replica. It is considered a measure of their relative abundance. d The results showed correspond to prediction using PSORTb algorithm, and when no output was obtained, Cello algorithm was applied and the result reported is indicated with a "*". OM- Outer membrane, CM- Cytoplasmic membrane, C- Cytoplasmic, P- Periplasmic, E- Extracellular, I- Indeterminate. Table 3. Proteins with statistical differential relative abundance of the UYSO10 strain exposed to root exudates, identified by the shotgun approach. Fold change p value Gene in UYSO10 Identified protein a COG category b Localization c Increased relative abundance in the presence of root exudates 1.79 0.00008 UYSO10_5873 ATP synthase F0 sector subunit b (EC 3.6.3.14) C MC 2.20 0.0004 UYSO10_4596 Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) C C 2.67 0.0081 UYSO10_0935 Aspartate ammonia-lyase (EC 4.3.1.1) E C 3.87 0.0013 UYSO10_1052 Fructose-1,6-bisphosphatase, type I (EC 3.1.3.11) G C 1.75 0.00001 UYSO10_1546 Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2) I C 3.24 0.0035 UYSO10_5421 LSU ribosomal protein L16p (L10e) J C 2.30 0.0105 UYSO10_2391 Outer membrane porin OmpF M ME 3.12 0.0121 UYSO10_1538 Outer membrane chaperone Skp (OmpH) precursor @ Outer membrane protein H precursor M P 2.09 0.0106 UYSO10_3810 Chemotaxis response - phosphatase CheZ N C 4.20 0.0123 UYSO10_5222 Aerotaxis sensor receptor protein N MC 1.50 0.0004 UYSO10_1850 Cell division trigger factor (EC 5.2.1.8) O C 2.30 0.0032 UYSO10_2197 Alkyl hydroperoxide reductase protein C (EC 1.11.1.15) O C 2.42 0.0259 UYSO10_0939 Heat shock protein 60 family co-chaperone GroES O C 2.86 0.0136 UYSO10_2199 Universal stress protein G T C Decreased relative abundance in the presence of root exudates 2.80 0.0246 UYSO10_2907 N-ethylmaleimide reductase (EC 1.-.-.-) C C 2.18 0.02 UYSO10_2694 Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24) C C 1.89 0.0058 UYSO10_3648 Aldehyde dehydrogenase (EC 1.2.1.3) C C 3.73 0.0229 UYSO10_3584 Allophanate hydrolase (EC 3.5.1.54) E C 3.46 0.0441 UYSO10_3583 Urea carboxylase (EC 6.3.4.6) E C 2.75 0.0422 UYSO10_3581 Urea ABC transporter, substrate binding protein UrtA E P* 3.00 0.0043 UYSO10_3416 Periplasmic Murein Peptide-Binding Protein MppA E P 2.93 0.0006 UYSO10_3304 ABC transporter periplasmic-binding protein YdcS E P 2.63 0.0244 UYSO10_5340 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) E C 2.14 0.0097 UYSO10_3047 Dipeptidyl carboxypeptidase Dcp (EC 3.4.15.5) E C 1.69 0.0013 UYSO10_5628 Oligopeptidase A (EC 3.4.24.70) E C 2.26 0.0089 UYSO10_2938 Adenosine deaminase (EC 3.5.4.4) F C 3.23 0.0044 UYSO10_5518 Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27) G C 2.89 0.0175 UYSO10_3704 Trehalase (EC 3.2.1.28) @ Periplasmic trehalase (EC 3.2.1.28) G P 2.42 0.0374 UYSO10_0252 Glucose-6-phosphate isomerase (EC 5.3.1.9) G C 2.39 0.0094 UYSO10_4192 Bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein G C 4.30 0.0241 UYSO10_0119 Outer membrane vitamin B12 receptor BtuB H ME 5.51 0.0102 UYSO10_0197 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) I C 1.65 0.0014 UYSO10_4361 Long-chain fatty acid transport protein I ME 5.29 0.00001 UYSO10_4543 Excinuclease ABC subunit B L C 2.58 0.0028 UYSO10_3747 Tail-specific protease precursor (EC 3.4.21.102) M MC 2.02 0.0045 UYSO10_1422 Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor M ME 1.98 0.0034 UYSO10_4265 Outer membrane porin OmpC M ME 1.78 0.002 UYSO10_1537 Outer membrane protein assembly factor YaeT M ME 3.64 0.0019 UYSO10_3853 Flagellar M-ring protein FliF N MC/ME* 3.26 0.003 UYSO10_3514 Methyl-accepting chemotaxis protein N MC 2.33 0.0378 UYSO10_3814 Methyl-accepting chemotaxis protein IV (dipeptide chemoreceptor protein) N MC 2.02 0.0007 UYSO10_2027 Methyl-accepting chemotaxis sensor/transducer protein N MC 6.22 0.0018 UYSO10_5347 Outer membrane stress sensor protease DegQ, serine protease O P 2.26 0.0278 UYSO10_1856 Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8) O P 3.43 0.0131 UYSO10_2599 Ferrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain P P 3.43 0.0233 UYSO10_3369 Uncharacterized protein YdcJ S C 2.67 0.0211 UYSO10_1105 FIG00975563: hypothetical protein S P* 2.38 0.0001 UYSO10_2026 domain of unknown function DUF1745 S C 1.70 0.0018 UYSO10_2023 response regulator receiver modulated metal dependent phosphohydrolase T C 2.35 0.0109 UYSO10_4584 Tol-Pal system beta propeller repeat protein TolB U P a The protein annotation is obtained from the search using the strain UYSO10 database. b COG categories: J-translation; L- replication, recombination and repair; O- chaperone molecules and related functions; M- structure and biogenesis of the cell envelope and outer membrane; N- secretion, mobility and chemotaxis; T- signal transduction; P- transport and metabolism of inorganic ions; C- energy production and conversion; G- carbohydrate transport and metabolism; E- transport and metabolism of amino acids; F- nucleotide transport and metabolism; H- coenzymatic metabolism; I- lipid metabolism; U- intracellular traffic and secretion; S- no functional prediction. c The results showed correspond to prediction using PSORTb algorithm, and when no output was obtained, Cello algorithm was applied and the result reported is indicated with a "*". OM- Outer membrane, CM- Cytoplasmic membrane, C- Cytoplasmic, P- Periplasmic. Supplementary Files renamedcfd82.docx Cite Share Download PDF Status: Published Journal Publication published 03 Dec, 2024 Read the published version in Plant and Soil → Version 1 posted Editorial decision: Major revisions 28 Apr, 2024 Reviewers agreed at journal 12 Mar, 2024 Reviewers invited by journal 11 Mar, 2024 Editor invited by journal 10 Mar, 2024 Editor assigned by journal 10 Mar, 2024 First submitted to journal 07 Mar, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4034332","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":278247182,"identity":"6f975f19-1606-41f4-821f-99d133946d39","order_by":0,"name":"Cecilia Taulé","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Cecilia","middleName":"","lastName":"Taulé","suffix":""},{"id":278247183,"identity":"c97b292c-8a55-430d-acd1-5938eeff9c15","order_by":1,"name":"Analía Lima","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Analía","middleName":"","lastName":"Lima","suffix":""},{"id":278247184,"identity":"8538952c-3f94-4577-9fca-a6f9f6036547","order_by":2,"name":"Martín Beracochea","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Martín","middleName":"","lastName":"Beracochea","suffix":""},{"id":278247185,"identity":"f0230c9d-44f5-4f4f-89e1-843a1b6d1c9d","order_by":3,"name":"Rosario Durán","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Rosario","middleName":"","lastName":"Durán","suffix":""},{"id":278247186,"identity":"ffc80650-b2ff-42c2-ab3b-414301cc3e2a","order_by":4,"name":"Federico Battistoni","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA8klEQVRIiWNgGAWjYBAC9hkg0gBEMDY+SDA4ABaVwKeF5wYDY8MBA5AixmYDErRAFLEBCWK0SDc/f/yhoK6OX7q5reJBwR15fgbmg7d5GA4nNuDSInPMEOiwwxKScw623UgweGY4s4Et2RqfFnuJBJCWAxIGNxJBWg4DvcNjJo3XFon0j0AtdWAtBSAt9gf4vxHQkgOyhRmshQFsCwMPG34tMmcKZ5wxOCw5c0ZiswTILzMOsxlbzjFIN8apRbp9w4eKP3X8/BLpDz/++AMMsfbmhzfeVFjL4tKCBTCDCAMGRxK0QIE9yTpGwSgYBaNguAIAliJcDKgAvQwAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0002-5648-1304","institution":"IIBCE","correspondingAuthor":true,"prefix":"","firstName":"Federico","middleName":"","lastName":"Battistoni","suffix":""}],"badges":[],"createdAt":"2024-03-08 01:27:22","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4034332/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4034332/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s11104-024-07112-9","type":"published","date":"2024-12-03T15:57:48+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":52580855,"identity":"6e59ddec-cd95-4939-b2fb-8efd735679be","added_by":"auto","created_at":"2024-03-13 08:18:07","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":612543,"visible":true,"origin":"","legend":"\u003cp\u003eRepresentative 2D-DIGE image of total protein extracts from strain \u003cem\u003eK. radicincitans\u003c/em\u003e UYSO10 with and without sugarcane root exudates. The resultant image is an overlay of strain UYSO10 growth with exudates (labeled with Cy3, green), without exudates (labeled with Cy5, red), and the internal standard (labeled with Cy2, blue). Arrows indicate spots with differential abundance between treatments.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/496debff06b8072810c633f8.png"},{"id":52580859,"identity":"f2a3ec7c-771f-4442-8fe3-01cc760cd8e2","added_by":"auto","created_at":"2024-03-13 08:18:07","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":88820,"visible":true,"origin":"","legend":"\u003cp\u003eDifferentially abundant proteins between strain \u003cem\u003eK. radicincitans\u003c/em\u003eUYSO10 exposed or not to root exudates. The volcano plot shows the rate of change versus the statistical significance for each protein. Each dot represents an experimental detected protein and blue dots correspond to proteins that satisfy the significance change rate criteria used.\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/5290aafc4635829383ebf284.png"},{"id":52580857,"identity":"280149dc-4785-4ae5-9ead-15f298f93ba0","added_by":"auto","created_at":"2024-03-13 08:18:07","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":99134,"visible":true,"origin":"","legend":"\u003cp\u003eSchematic representation of the main pathways of C metabolism identified in the strain UYSO10 exposed to root exudates.\u003c/p\u003e\n\u003cp\u003eThe pathways marked with black: are constitutively expressed in both conditions, green: up-regulated, and red: down-regulated in the presence of toot exudates respectively. The broken black arrows indicate possible pathways for which the expressed enzyme was not found. Asterisks: enzyme expressed only in the presence (green) or absence (red) of root exudates. When the same pathway is indicated with more than one indication, it refers to the fact that proteins or subunits with different tendencies were observed. The analysis of each subunit of the enzyme is represented. Gray boxes indicate a possible function of the protein.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/37261c160552f457cb418cd1.png"},{"id":52580856,"identity":"706b861f-4fa6-4727-8952-d78f1b869f23","added_by":"auto","created_at":"2024-03-13 08:18:07","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":85093,"visible":true,"origin":"","legend":"\u003cp\u003eSchematic representation of the possible N-sources and their metabolization, identified in the strain UYSO10 under the conditions evaluated. The proteins and pathways in black: are constitutively expressed, in green: are up-regulated, and in red: down-regulated in the presence of root exudates respectively.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/bc11f8e31534c0424550f251.png"},{"id":52581113,"identity":"a5cb880b-a5b3-46e8-bb25-a84139e6ec5f","added_by":"auto","created_at":"2024-03-13 08:26:07","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":129972,"visible":true,"origin":"","legend":"\u003cp\u003eSchematic representation of strain UYSO10 proteins associated with cell envelope, signal transduction, mobility, and adhesion in the presence of root exudates. Green and red proteins are up- and down-regulated in the presence of root exudates respectively. Gray boxes indicate a possible function\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/b5fc7dadba1e2d2c43603a04.png"},{"id":70964826,"identity":"1be6d50c-7a96-4dd2-b20b-260ac71111ef","added_by":"auto","created_at":"2024-12-09 16:16:20","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2198337,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/03293319-d810-478e-a183-ea6f80177aea.pdf"},{"id":52580860,"identity":"9388669f-77bc-4785-ab52-ca1143603ffb","added_by":"auto","created_at":"2024-03-13 08:18:08","extension":"docx","order_by":7,"title":"","display":"","copyAsset":false,"role":"supplement","size":176230,"visible":true,"origin":"","legend":"","description":"","filename":"renamedcfd82.docx","url":"https://assets-eu.researchsquare.com/files/rs-4034332/v1/6d218be4ef6071743eefd422.docx"}],"financialInterests":"","formattedTitle":"Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate","fulltext":[{"header":"Introduction","content":"\u003cp\u003eThe plant holobiont concept has an integrative view on the understanding of the plant phenotype. This holobiont is defined as the assemblage of the plant and all the macro and microorganisms (bacteria, archaea, fungi, viruses, protozoa, and algae), that live on or inside the plant, being the plant phenotype the result of the interaction of all of them and the environment (Rosenberg and ZIlber-Rosenberg 2016). One of the main components of the plant holobiont is its associated microbiota communities, which play a key role in the growth and health status of the plant (Berg et al. 2020). Particularly, the endophytic bacterial microbiota, which refers to those bacteria communities that inhabit the internal tissues of the plant, are involved in key processes such as the modulation of plant secondary metabolism (i.e. by producing indole acetic acid, gibberellin, cytokinins, ACC-deaminase), the improvement of nutrient acquisition (by biological N\u003csub\u003e2\u003c/sub\u003e fixation), and enhancing plant defenses and tolerance to abiotic stresses (Adeleke et al. 2021; Taul\u0026eacute; et al. 2021). Studying the mechanisms involved in the interactions between the different components of the endophytic microbiota, as well as with their host, is of fundamental importance to understanding the resulting phenotype of the plant holobiont. Among the main sources of endophytic bacteria are the soil bacteria and the endophytic bacteria associated with the seeds (Taul\u0026eacute; et al. 2021). During plant development, different signals are exchanged between the components of these microbiotas, as well as with the host plant, triggering a series of bacterial activities resulting in the establishment of the following endophytic bacterial microbiotas\u0026nbsp;(Hassani et al. 2018; Taul\u0026eacute; et al. 2021).\u0026nbsp;Particularly, the colonization of plants by soil bacteria begins when they are attracted to the rhizosphere by chemical signals exuded by the plant (Kandel et al. 2017). Subsequently, the bacteria colonize the surface of the rhizoplane, particularly at the sites of active exudation (emergence of lateral roots or hairs, and root tips); to finally enter the internal tissues (Kandel et al. 2017). \u0026nbsp;Although the processes of colonization and infection of endophyte bacteria are well documented, the molecular bases of the mechanisms involved in the plant-endophyte interaction are still poorly understood.\u003c/p\u003e\n\u003cp\u003ePreviously, it was shown that sugarcane cultivars grown in Uruguay can obtain significant amounts of N through the biological nitrogen-fixation (BNF) process. From those N\u003csub\u003e2\u003c/sub\u003e-fixing cultivars, a collection of associated plant growth-promoting bacteria (PGPB) was constructed and characterized (Taul\u0026eacute; et al. 2012). Through greenhouse assays, it was shown that strain UYSO10 promotes the growth of micropropagated and cutting sugarcane plants cv. LCP 85-384 (LCP) (Taul\u0026eacute; et al. 2016, 2019). In addition, the strain UYSO10 was defined as a diazotroph and as a true endophyte of sugarcane plants (Taul\u0026eacute; et al. 2012, 2016). Subsequently, the genome was sequenced, analyzed, and the strain was defined as belonging to the species \u003cem\u003eKosakonia radicincitans\u003c/em\u003e (Beracochea et al. 2019).\u003cem\u003e\u0026nbsp;In silico\u003c/em\u003e genome analysis showed the presence of two nitrogenase-encoding operons, Mo- and Fe-nitrogenase (Beracochea et al. 2019). Simple and double mutants in the nitrogenase structural \u003cem\u003enifH\u0026nbsp;\u003c/em\u003eand \u003cem\u003eanfH\u0026nbsp;\u003c/em\u003egenes were constructed, and their \u003cem\u003ein vitro\u003c/em\u003e characterization showed that both enzymes were active. Moreover, by inoculation of these mutants into sugarcane plants in greenhouse assays, it was demonstrated that the BNF process is involved in the PGP observed (Taul\u0026eacute; et al. 2019).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThis work aims to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the strain \u003cem\u003eKosakonia radicincitans\u003c/em\u003e UYSO10 and sugarcane plants through two orthogonal proteomic approaches.\u003c/p\u003e"},{"header":"Materials and methods","content":"\u003cp\u003e\u003cstrong\u003eBacterial strain and plant cultivar\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eEndophytic bacterial strain \u003cem\u003eK. radicincitans\u003c/em\u003e UYSO10 was isolated from sugarcane cultivar FAM 81-72 (Taulé et al. 2012). Micropropagated sugarcane plants cv. LCP were produced and supplied by the Biotechnology Unit, Instituto de Investigaciones Agropecuarias (INIA) as previously described (Taulé et al. 2016). In the interaction studies, micropropagated plants were cultivated in 1/10 MS liquid culture medium supplemented with Staba vitamins, and without N source (MS-N)\u0026nbsp;(Reis et al. 1999; Taulé et al. 2016).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eBacterial exposure to root exudates\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe strain UYSO10 was grown in 20 ml of TY culture medium for 24 h. Cells were harvested by centrifugation, the pellet was suspended in MS-N culture medium, and incubated for 16 h at 30 ºC (adapted cells). In parallel, micropropagated sugarcane plants were grown in flasks containing 40 ml of MS-N culture medium. On the 3rd day plants were removed and the culture media was filtered (MSE medium). Adapted cells were inoculated to flasks containing 40 ml of MS-N or MSE media (1.25 x 10\u003csup\u003e9\u003c/sup\u003e cells/ml), and incubated at 30 °C. After 6 h of incubation, cells were harvested by centrifugation for 10 min at 6,000 g at 4 ºC, the pellet was washed and frozen at -20 ºC until use. Four independent biological replicates were used, where a biological replica was considered as the pool of cells obtained from 4 flasks.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eProtein extraction and quantification\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eBacterial pellets obtained were suspended in extraction buffer containing 8 M urea, 2 M thiourea, 4% w/v CHAPS, and 10 mM dithiothreitol (DTT), with agitation for 30 min at 4 ºC. Samples were sonicated 3 times for 15 s on ice and again incubated with agitation for 30 min. Finally, samples were centrifuged for 20 min at 20.000 g and 4 °C, and the supernatants were conserved at -20°C for further analysis.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eProtein concentration was estimated using the Bradford method (Bradford 1976), and protein quality extraction was evaluated by SDS-PAGE (12 % acrylamide).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2D-Difference Gel Electrophoresis (DIGE)\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFor DIGE analysis, 300 µl of 10% trichloroacetic acid (TCA) were added to 200 µg of protein samples and incubated for 15 min on ice with agitation. Subsequently, 300 µl of 0.2% deoxycholate were added to the samples, centrifuged at 15.000 g for 5 min at 4 ºC, suspended in 40 µl of 0.2% deoxycholate and additionally centrifuged for 5 min. Pellets obtained were suspended in 25 µl of H\u003csub\u003e2\u003c/sub\u003eO with vigorous agitation and incubated immediately with 1 ml of pre-cooled acetone overnight at –20 ºC. After that, samples were centrifuged at 15.000 g for 10 min at 4 °C, and the pellets obtained were dry at room temperature and suspended in 40 µl of solubilization buffer (8 M urea, 2 M thiourea, 4 % w/v CHAPS, 30 mM Tris pH 8.5) with agitation for 1 h on ice. Finally, the samples were centrifuged at 20.000 g for 15 min at 4 ºC, and the supernatant was collected.\u003c/p\u003e\n\u003cp\u003eFor protein labeling with the CyDye DIGE fluor, the GE Healthcare Kit (GE, Chicago USA) was used. Briefly, 50 µg of each protein sample (bacteria exposed to or not to plant exudates) were labeled with 400 pmol of Cy3 and Cy5 fluor. An internal standard was prepared including 25 µg of each protein sample and labeled with the Cy2 fluor. To compensate for any labeling bias, Cy3 and Cy5 were alternatively used to label the incubated and not incubated protein samples, while Cy2 was exclusively used for labeling the standard. The labeling reaction was carried out for 30 min, in the dark on ice, and stopped by incubation with 10 mM lysine.\u003c/p\u003e\n\u003cp\u003eEqual amounts of sample (from different experimental groups and labeled with different dyes) and the internal standard were mixed and separated in an individual 2-D electrophoresis. For that samples were loaded into 24 cm IPG-strips (pH 3–10 non-linear) on IPGbox (GE Healthcare, Chicago USA), and they were separated by isoelectric focusing on an IPGphor III equipment (GE Healthcare, Chicago USA), employing the recommended voltage profile. Next, disulfide bonds were reduced with 1% w/v dithiothreitol and subsequently alkylated with 4,7% w/v iodoacetamide. The second dimension was performed on a 12 % polyacrylamide SDS-PAGE hand-cast gel, using the Ettan DALT six Electrophoresis System (GE Healthcare), equipped with the Multitemp III cooling unit (GE Healthcare) thermostatted at 20 ºC. The current applied was settled for the first 45 min at 2 W per gel and then increased for 4-5 h to 17 W per gel.\u003c/p\u003e\n\u003cp\u003eGels were scanned on a Typhoon FLA 9500 scanner (GE Healthcare), using the ImageQuant TL v8.1 software (GE Healthcare) at a resolution of 100 μm, and using the laser wavelength and the filter settings indicated for each dye. The photomultiplier tube voltage was adjusted on each channel to give maximum pixel values below saturation levels (60,000–90,000 counts). Subsequently, the images were exported to the DeCyder 2D Differential Analysis Software 7.2 program (GE Healthcare) for analysis. Then, all the spots of each gel were detected, quantified, and normalized with the internal standard using the Difference In Gel Analysis module. After that, the relative abundance of each spot between the gels was compared by using the Biological Variation Analysis module. Finally, those spots that presented a change rate greater than 1.5 folds and were statistically supported with a Student test (p-value of 0.05), were selected for identification.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eProtein identification by MALDI-TOF/TOF\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eGels were fixed for 30 min with ethanol: acetic acid: water (5:1:4) and stained overnight with a solution of Coomassie Blue G-250 in ethanol (8% w/v ammonium sulfate, 0.8% v/v phosphoric acid, 0.08% w/v Coomassie Blue G-250, 20% v/v ethanol). The excess dye was removed by successive washings with distilled water. The spots recognized as differential were located on the gel and carefully excised.\u003c/p\u003e\n\u003cp\u003eThe spots were processed as previously reported (Lima et al. 2021), and analyzed by mass spectrometry in a 4800 MALDI-TOF/TOF equipment (Abi Sciex).\u0026nbsp;Mass spectra were calibrated using a mixture of peptides standard (Applied Biosystems) and trypsin autolysis products. Some peptides from all protein spots were selected for MS/MS analyses using the following settings: 8 kV and 15 kV for sources 1 and 2, respectively. \u0026nbsp;The spectra obtained were compared with the proteome of the strain UYSO10, using the MASCOT search server (\u003cu\u003ehttp://www.matrixscience.com\u003c/u\u003e) in Sequence Query Mode. The search parameters in Mascot were: i- allowed trypsin cut jumps: 1; ii- variable modifications: methionine oxidation and fixed modification: carbamidomethylation of cysteines; iii- peptide mass tolerance: 0.08 Da and MS/MS tolerance: 0.3-0.5 Da. The \u003cem\u003em/z\u003c/em\u003e values used correspond to the monoisotopic values. The identification of a protein was considered when the Mascot protein score was statistically significant (p \u0026lt; 0.05) and at least one fragmentation was assigned with a significant Mascot peptide ion score (p \u0026lt; 0.05).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eNano LC-MS/MS analysis and protein identification\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eProtein samples (15 µg) were loaded onto a pre-made polyacrylamide gel with a 4-12% non-linear gradient (NuPAGE, MES System, Invitrogen), and run for 1 h 15 min under a constant current of 160 V. Next, the gel was fixed and stained with Coomassie Blue G-250 as described above. Each gel lane was cut into 6 fragments, which were first destained with a solution of 50 % acetonitrile (ACN), 0.2 M ammonium bicarbonate (1:1) in ultrapure water for 1 h, with agitation at 30°C. Then, each gel fragment was reduced with 10 mM DTT for 1 h at 56 °C and alkylated with 55 mM iodoacetamide for 45 min at room temperature. Subsequently, the samples were washed with 0.2 M ammonium bicarbonate pH 8, for 15 min and dehydrated by two incubations of 10 min with ACN 100 % under agitation. Finally, the ACN was removed and the gel fragments dried completely with the tube open at room temperature in a laminar flux cabinet. \u003cem\u003eIn gel\u003c/em\u003e-digestion with trypsin was carried out overnight at 37 °C and peptide extraction was obtained by vigorously incubation (1400 rpm) the samples for 2 h on phase B (60 % ACN, 0.1 % formic acid), at 30 ºC. Peptide samples were vacuum dried, the pellet resuspended with 0.1 % formic acid (phase A), with sonication for 15 s, and centrifuged for 20 min at 16.000 g and 4 ºC. The supernatants were kept as samples.\u003c/p\u003e\n\u003cp\u003eSamples were separated using a nano-HPLC system (EASY-nLC 1000, Thermo Scientific), coupled to an LTQ Velos mass spectrometer (Thermo Scientific). Peptide mixtures were injected into an Acclaim PepMap 100, Nanoviper C18 nano-trap column (75 μm × 2 cm, Thermo Scientific) and separated on a PepMap RSLC C18 (50 μm × 150 mm, Thermo Scientific). Peptide elution was achieved with a flow rate of 250 nl/min and a 100 min gradient from 0 % to 50% of mobile phase B. Peptide analysis was performed in an LTQ Velos nano-ESI linear ion trap equipment (Thermo Scientific) in a data-dependent acquisition mode. Xcalibur 2.1 was used for data acquisition in two steps: 1. acquisition of full MS scan in the positive ion mode with \u003cem\u003em/z\u003c/em\u003e between 300 and 1800 Da, 2. sequential fragmentation of the ten most intense ions, using a dynamic exclusion list (exclusion duration 30 s). A normalized collision energy of 35 and an isolation width of 2 \u003cem\u003em/z\u003c/em\u003e were set. The activation Q was set at 0.25, and the activation time was 15 ms. MS source parameters were set as follows: 2.3 kV electrospray voltage and 260 ◦C capillary temperature.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eFor data analysis, the program PatternLab for Proteomics v4.0 (http://www.patternlabforproteomics.org/) was used (Carvalho et al. 2016). First and using the proteome of the strain UYSO10, a new database of the target reverse type was generated, which included the 127 most common contaminants (e.g. keratin, BSA, among others). Next, the identification of the peptides in the sample was carried out by comparing the data obtained in the mass spectrometer, with the generated database using the\u0026nbsp;Comet Search Engine. In this procedure the following parameters were taken into account: i- precursor mass tolerance: 800 ppm\u0026nbsp;from the measured precursor \u003cem\u003em/z\u003c/em\u003e, ii- enzyme: trypsin with full specificity, accepting a maximum of 2 cuts skipped by the enzyme, iii- fixed modification: carbamidomethylation of cysteines and variable: oxidation of methionine, and iv- at most two variable modifications per peptide. Subsequently,\u0026nbsp;peptide spectrum matches were filtered using the Search Engine Processor (SEPro), and acceptable false discovery rate (FDR) criteria were set as follows: \u0026nbsp;≤ 3% at the spectra level, ≤ 2% at the peptide level, ≤ 1% at the protein level, and a minimum of two sequences peptides assigned by identified protein.\u0026nbsp;PatternLab’s statistical model for the Approximately Area Proportional Venn Diagram module was used to compare conditions and determine proteins uniquely detected in each experimental condition, using as a criterion that they are present in at least 3 of 4 replicates of the treatment, but in 0 or 1 of the other treatment. PatternLab’s T-Fold module was used to detect proteins present in both conditions at significantly different levels by spectral count analysis and (p-value of 0.05).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eProteomic data availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eProteomics data generated in the present work have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier\u0026nbsp;PXD050409.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEvaluation of the capacity to grow on different C and N sources\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe utilization of different carbon sources by the strain UYSO10 was evaluated using the KB009 HiCarbohydrate™ kit (Himedia). Complementary five microliters of strain UYSO10 washed cells, from an overnight LGI medium culture, were inoculated on plates containing LGI medium with C and N substitution. The sources to be tested were added at the final concentrations as per the original protocol (Baldani et al. 1984). The C sources tested were: non-refined sugar, sucrose, glucose, mannitol, lactose, and the organic acids: malate, succinate, fumarate, pyruvate, and acetate. In addition, 100 and 200 g/l of sucrose and non-refined sugar were also tested. The N sources tested were: ammonia, nitrate, L-asparagine, glutamic and aspartic acids, and urea. Additionally, the use of apoplast and root exudates was tested both as C and N sources. The apoplast was obtained by centrifugation, from stem internodes of field sugarcane plants (Dong et al 1994), while root exudates were obtained as described above. All the aforementioned determinations were performed in triplicate.\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003eProteome response of strain UYSO10 to sugarcane root exudates analyzed by DIGE\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe gel images analyzed detected 1950 spots, of which 59 (3% of the total) showed differential relative abundance (34 spots increased, 25 spots decreased in the presence of exudates) (Figure 1 and S1). From this set of differentially expressed proteins, 34 were located in the stained gel and excised for their identification by MALDI-TOF/TOF. From that, 16 were identified and validated by comparing the theoretical mass and the predicted isoelectric point observed experimentally (Table 1).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eProteins upregulated in the presence of root exudates were associated with carbohydrate and amino acid metabolism (Table 1). On the other hand, downregulated proteins were related to membrane biogenesis, signal transduction, carbohydrate metabolism, and adaptation to stress.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eProteome response of strain UYSO10 to sugarcane root exudates analyzed by nano LC-MS/MS\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eUsing a shotgun proteomic approach, a total of 897 proteins were identified, representing 15.1% of the genome CDS. From that, 4 and 59 proteins were exclusively identified in the presence and absence of roots exudate respectively (Table 2).\u003c/p\u003e\n\u003cp\u003eFrom the total proteins evaluated, 834 were identified in both conditions, where 50 proteins showed differential relative abundance. Among those, in the presence of exudates 14 proteins (0.2 %) were identified as upregulated, while 36 proteins (0.6 %) as downregulated (Figure 2 and Table 3). Taking together unique proteins showing differential relative abundance, we observed that in the presence of exudates, 0.3 % of the total proteome was up-regulated, while 1.6 % was down-regulated (Figure S1).\u003c/p\u003e\n\u003cp\u003eThe cell localization of the differential expressed proteins was achieved using PSORTB and Cello tools. Proteins were classified into cytoplasmic (55), cytoplasmic membrane (19), periplasmic (15), outer membrane (7), and extracellular (3) (Tables 2 and 3). Concerning COG functional categories, differentially expressed proteins were mainly associated with carbohydrate and amino acid transport and metabolism, biogenesis and membrane structure, and energy production and conversion (Tables 2 and 3, Figure S2).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eStrain UYSO10's ability to use different C and N sources\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eResults indicate that strain UYSO10 could utilize a great variety of C sources including sucrose, non-refined sugar, trehalose, glucose, fructose, mannitol, xylose, maltose, dextrose, L-arabinose, mannose, sorbose, acetate, citrate, malate, pyruvate, succinate, fumarate and salicin (Table S1). Concerning the ability to use different N-sources, results showed that strain UYSO10 can use all the tested compounds (Table S1). Finally, results also showed that the strain UYSO10 can use either the sugarcane root exudates, as well as the apoplast as a sole C and N-sources (Table S1).\u0026nbsp;\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003e\u003cstrong\u003eRoots exudate in the establishment of the plant-bacteria interaction\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePlants exudate between 20-40 % of their photosynthetic products to the soil, which consists mainly of complex sugars, amino acids, organic acids, extracellular enzymes, phenols, vitamins, and nitrogenous macromolecules\u0026nbsp;(Haichar et al. 2014; Canarini et al. 2019; Vives-Peris et al. 2020).\u0026nbsp;Compounds present in root exudates serve as a source of C and N for soil bacteria, shaping the rhizospheric community's structure, diversity, and functionality (Haichar et al. 2014). Particularly, some exudates compounds serve also as signals including sugars, amino acids, carboxylic acids (succinate, fumarate, malate), and aromatic compounds (luteolin, catechols, acetosyringone, among others), which induce a flagella-dependent chemotaxis in soil bacteria towards the roots (Haichar et al. 2014). \u0026nbsp;The main points of root exudates release are the root hairs, the apical zone, as well as the emergence sites of secondary roots, being the preferred colonization sites for the rhizospheric and endophytic bacteria\u0026nbsp;(Haichar et al. 2014; Canarini et al. 2019; Compant et al. 2021).\u0026nbsp;Diverse physical, chemical, and biological environmental factors such as the presence of biotic and abiotic stress, as well as the structure of the soil, modulate quantitatively and qualitatively the composition of the exudate\u0026nbsp;(Canarini et al. 2019; Vives-Peris et al. 2020).\u0026nbsp;At the same time, the exudation of certain specific molecules depends in part on the recognition of molecular signals secreted by soil bacteria in response to signals previously secreted by the plants (Mark et al. 2005; Witzel et al. 2017). In this sense, it is considered that the molecular signaling between bacteria and plants plays a fundamental role in establishing beneficial and pathogenic interactions. However, the molecular mechanisms underlying plant-endophytic bacterial interactions are poorly understood (Haichar et al. 2014; Coskun et al. 2017).\u003c/p\u003e\n\u003cp\u003ePreviously, it was demonstrated that the strain UYSO10 mainly colonized the emergence zone of lateral roots of sugarcane plants (Taulé et al. 2016). Therefore in this work, the bacterial response to sugarcane root exudates was evaluated in the same culture medium (Taulé et al. 2016). The MS-N culture medium employed has a low C source concentration (sucrose 2 g/l), and does not have an N-source which favors the interaction with diazotrophic bacteria\u0026nbsp;(Reis et al. 1999; Lery et al. 2011; Taulé et al. 2016).\u0026nbsp;But this low nutrient condition also constitutes a stress situation for both plants and bacteria and particularly defines a specific plant exudation profile. For instance, when N-nutrients are poorly available, plants stimulate lateral root proliferation, and reduce amino acid exudation, while increasing organic acid exudation\u0026nbsp;(Canarini et al. 2019; Vives-Peris et al. 2020).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eProteomic response of the endophytic bacteria \u003cem\u003eKosakonia radicincitans\u003c/em\u003e UYSO10 to root exudates\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn the present work, two proteomic strategies were used to address the strain UYSO10 response to sugarcane root exudates. Results obtained showed that all the proteins identified by applying the DIGE strategy were identified using nano LC-MS/MS. However, not all proteins showed the same expression trend in both approaches (Table S2). These differences may be associated with the nature of the techniques employed. The DIGE technique separates proteins and their proteoforms, and eventually being able to identify post-translational modifications; however, this technique is not sensitive to low-concentration proteins. On the other hand, by using the nano LC-MS/MS technique, the total populations of each protein (all the proteoforms), are quantified and it is sensitive to identify proteins expressed in lower concentrations. The following analysis integrates the results obtained by both proteomics approaches used in this work.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eNutrient transport and metabolism\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003eAs strain UYSO10 is an endophytic bacterium, it was expected that in the first steps of the interaction with the plants, it would be able to capture and metabolize the compounds exuded by the plant either as a nutritional source or as a signal.\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eC metabolism.\u003c/em\u003e Strain UYSO10 constitutively expressed proteins associated with the transport and metabolization of different C-sources (Figure 3). Interestingly, in the presence of root exudates, no evidence of a specific C-source use was detected since no upregulation of any transporter was identified under the conditions evaluated. On the other hand, in the absence of exudates, proteins associated with the transport and metabolization of different C-sources were up-regulated, including fructose and acetate (Figure 3). The main C-source in the MS-N medium is sucrose and it seems that the monomer glucose is used in both conditions evaluated, while fructose is also used in the absence of root exudate. It should be noted that the strain UYSO10 can grow using sucrose, glucose, and fructose as the sole C-source (Table S1). Interestingly, the same behavior of differential use of sucrose monosaccharides has been reported in the strain \u003cem\u003eE. coli\u003c/em\u003e W grown under low concentrations of sucrose (Sabri et al. 2013). Thus, it seems that in the absence of root exudates the strain UYSO10 expresses a battery of C-transporters to take any potential C-source from the growth medium. While, in the presence of root exudate the strain UYSO10 uptake the C-sources using the constitutively expressed C-transporters. \u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn the presence of root exudates, some enzymes of the Krebs cycle and energy metabolism (e.g. F1F0-ATPase) were up-regulated, suggesting the bacteria is metabolically active (Figure 3). Particularly, in similar work, the succinyl-CoA ligase enzyme was also identified as up-regulated in the strain \u003cem\u003eGluconacetobcter diazotrophicus\u003c/em\u003e Pal5 growing in co-culture with sugarcane plants (Dos Santos et al. 2010). This expression was correlated with the production of EPS and certain succinic-glycans, indicating a possible role in the establishment of bacteria-plant associations (Dos Santos et al. 2010).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eN metabolism\u003c/em\u003e. Although the strain UYSO10 is diazotrophic, and the genome encodes for two nitrogenases (Beracochea et al. 2019), the enzymes were not detected, probably due to the presence of oxygen in both conditions evaluated (de Bruijn 2015). Interestingly, the down-regulation of specific proteins related to the uptake of different N-sources was observed in the presence of root exudates. This correlates with a shift in the N-sources disponibility, from an N-limiting to an N-no-limiting situation due to the presence of easily available and assimilable N-compounds present in the roots exudates (Zimmer et al. 2000). Particularly, it seems that one of the N sources used in the presence of root exudates is aspartate (Figure 4, Tables 1 and 3). \u0026nbsp;This correlates with the fact that aminoacids are the main N-source in the sugarcane apoplast, being the aspartic acid the fourth most abundant (Tejera et al. 2006); as well as the availability of the strain UYSO10 to use aspartate as a sole N-source\u0026nbsp;(Table S1).\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eOsmotic stress.\u0026nbsp;\u003c/em\u003eThe proteomic analysis showed the constitutive expression of the gluconeogenesis pathway in both conditions evaluated and the up-regulation of two of its enzymes in the presence of root exudates (Figure 3). This observation, together with the repression of the synthesis of structural components such as glycogen, cellulose, and their derivatives, suggests that the strain UYSO10 may be synthesizing trehalose in the presence of root exudates (Figure 3). Since the synthesis and storage of trehalose was described as associated with osmotic stress, it is likely that the strain UYSO10 detects the presence of the plant from the exudates components and prepares its metabolism for future osmotic changes during the interaction (Ormeño-Orrillo et al. 2012). It is important to emphasize that strain UYSO10 was isolated from plates containing LGI-P culture media (LGI with 100 g/l of sucrose) and can grow on LGI medium containing 200 g/l of sucrose, as well as non-refined sucrose. From these results, strain UYSO10 could be considered an osmotolerant bacteria. In addition, a subunit of the first enzyme in the biosynthesis pathway of fatty acids (acetyl-CoA-carboxylase), was also identified as up-regulated in the presence of root exudates (Figure 3). \u003cem\u003eGluconacetobacter diazotrhophicus\u0026nbsp;\u003c/em\u003ePal5 mutants in another subunit of this enzyme are affected at high osmotic environments, highlighting its role in the adaptation to osmotic stress (Leandro et al. 2021). Moreover, fatty acids and modified fatty acids were described as important molecules during plant colonization by microorganisms (Pohl and Kock 2014; Uranga et al. 2016). More studies are necessary in the strain UYSO10 to understand the role of fatty acids during the plant-bacteria interaction.\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eMobility and chemotaxis.\u0026nbsp;\u003c/em\u003eThe bacterial transition from a mobile state to biofilm formation requires the expression of transcriptional regulators, which respond to environmental signals such as root exudates\u0026nbsp;(Han et al. 2023; Liu et al. 2024). \u0026nbsp;\u0026nbsp;In this sense in the presence of root exudates, the strain UYSO10 up-regulated the universal stress protein G, as well as the stringent response regulator DksA and the CheZ protein (Figure 5). Those proteins are associated in bacteria with an increase in the formation of aggregates and a decrease in chemotaxis and motility (Nachin et al. 2005; Min and Yoon 2020). Moreover, under the conditions mentioned, the structural protein of the flagellum FliF and four methyl-accepting chemotaxis proteins (MCP) were identified as down-regulated. The repression of genes linked to flagellum synthesis in plant-associated bacteria has been postulated as a strategy to evade plant defenses, since some of them like flagellin, were described as MAMP (Mark et al. 2005; Cheng et al. 2009; Shidore et al. 2012; Paungfoo-Lonhienne et al. 2016). However, the inhibition of the flagellum is not universal, for instance, in \u003cem\u003eH. seropedicae\u003c/em\u003e SmR1 growing in the presence of sugarcane extracts, the up-regulation of flagellin was reported, and associated with plant colonization and the formation of biofilms (Cordeiro et al. 2013). Probably, a fine-tuning of the flagellar apparatus during the endophytic bacterial colonization takes place intending to avoid the plant defense and allow root colonization.\u003c/p\u003e\n\u003cp\u003eFinally, the up-regulation of the aerotaxis sensory receptor (AerC) in the presence of root exudates, together with the low concentration of N-sources in the culture medium, could indicate that the strain UYSO10 is searching for a microaerophilic condition that allows the BNF process, as was reported in other diazotrophs (Yao and Allen 2007; Xie et al. 2010).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eBacterial cell envelope\u0026nbsp;\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003eMembrane proteins play an important role in processes such as signal transduction, and transport, as well as in cell-cell interaction, among others. The results obtained showed that in the presence of root exudates, the strain UYSO10 presented large changes in the expression of proteins associated with the biogenesis and structure of the cell envelope, suggesting a strong reorganization and therefore adaptation (Figure 5). Probably, some of these changes are associated with a potential compatible interaction with the plant perceived by the bacteria (Balsanelli et al. 2016; Pankievicz et al. 2016). For example, OmpF and OmpH proteins were up-regulated in the presence of root exudates. Both proteins were previously reported as up-regulated in \u003cem\u003ePseudomonas putida\u0026nbsp;\u003c/em\u003eand \u003cem\u003eG. diazotrophicus\u0026nbsp;\u003c/em\u003ein the presence of root exudates and co-culture with sugarcane respectively. The proposed role for those proteins includes the adhesion to the plant cell wall, the transport of small molecules like ACC, as well as receptors, and chaperon, beyond others (Cheng et al. 2009; Lery et al. 2011).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eOn the other hand, several membrane structural proteins (OmpC, OmpA, SecE, and TolB), and membrane biogenesis proteins (YaeT, MsbA, LpoA), as well as proteins associated with the synthesis and transport of LPS, were down-regulated in the presence of root exudates. Many of them have been described as immunogenic antigens, so their down-regulation could be linked to preventing the activation of the plant's immune system (Boller and Felix 2009).\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eChaperones and stress-related functions\u0026nbsp;\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003ePlant-bacteria interaction and nutrient starvation could be considered stressful situations for the bacteria. Therefore in the context of the experimental conditions evaluated, the identification of proteins related to chaperones and stress was expected. In this sense, in the presence of root exudates was identified as up-regulated the stringent response regulator DskA and the chaperone Heat shock GroES protein (Figure 5). The versatile role of the DskA regulator including its role in the virulence of the strain \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e was described (Min and Yoon 2020). The chaperons GroES and GroEL were identified as up-regulated in the strain \u003cem\u003eG. diazotrophicus\u003c/em\u003e, in co-culture with sugarcane plants (Dos Santos et al. 2010; Lery et al. 2011). In addition, the expression of the GroEL protein in \u003cem\u003eSinorhizobium meliloti\u003c/em\u003e has been associated with the regulation of the \u003cem\u003enod\u003c/em\u003e genes (Ogawa and Long 1995). Together these reports suggest that during the strain UYSO10-sugarcane interaction, the GroES chaperones could be involved in a specific adaptation and survival response.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eFinally and in the presence of root exudates, was identified as up-regulated the Alkyl hydroperoxide reductase protein C (AhpC) (Figure 5), an oxidoreductase protein described as involved in cellular and redox homeostasis (Mongkolsuk et al. 2000). The up-regulation of this enzyme was also identified in the endophytic strain \u003cem\u003eBurkholderia phytofirmans\u003c/em\u003e growing endophytically in potato plants (Sheibani-Tezerji et al. 2015). Moreover, the expression of several antioxidant enzymes has been observed in endophytic strains grown in co-culture with their host plants (Dos Santos et al. 2010; Lery et al. 2011; Alquéres et al. 2013). Together these reports suggest a probable role of the protein AhpC in the antioxidant response during the interaction with sugarcane plants.\u0026nbsp;\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003eExposure of strain UYSO10 to sugarcane root exudates induces quantifiable changes in the bacterial proteome. Those changes include the remodeling of several pathways toward the metabolic adaptation to the nutrients present in the root exudates. the change from a planktonic lifestyle to the formation of bacterial aggregates, as well as changes in the bacterial membrane. Together are correlated with the necessary steps for bacterial adaptation to the rhizosphere conditions toward rhizoplane colonization and root infection.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis work was supported by grants from the Uruguayan Fund for the Promotion of Agricultural Technology (Fondo de Promoci\u0026oacute;n de Tecnolog\u0026iacute;a Agropecuaria FPTA-275 and 331-INIA), the Uruguayan Program for the Development of the Basic Sciences (Programa de\u0026nbsp;Desarrollo de las Ciencias B\u0026aacute;sicas-PEDECIBA), the Posgrade\u0026nbsp;Academic Commission-UdelaR (Comisi\u0026oacute;n Acad\u0026eacute;mica de Posgrado),\u0026nbsp;and the Uruguayan National Agency for Innovation and Research (Agencia Nacional de Innovaci\u0026oacute;n e Investigaci\u0026oacute;n-ANII). The authors are very grateful to Ing. Agr. Fernando Hackembruch from the\u003c/p\u003e\n\u003cp\u003eAgriculture Department of the Alcoholes Uruguay S.A., and Alicia Castillo from the Biotechnology Unit, Estaci\u0026oacute;n Experimental Wilson Ferreira Aldunate, Instituto de Investigaciones Agropecuarias (INIA), Uruguay.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting Interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors have no relevant financial or non-financial interests to disclose\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eAdeleke BS, Babalola OO, Glick BR (2021) Plant growth-promoting root-colonizing bacterial endophytes. Rhizosphere 20:100433. https://doi.org/10.1016/j.rhisph.2021.100433\u003c/li\u003e\n\u003cli\u003eAlqu\u0026eacute;res S, Meneses C, Rouws L, et al (2013) The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by \u003cem\u003eGluconacetobacter diazotrophicus\u003c/em\u003e PAL5. Mol Plant Microbe Interact 26:937\u0026ndash;45. https://doi.org/10.1094/MPMI-12-12-0286-R\u003c/li\u003e\n\u003cli\u003eBaldani JI, Baldani VLD, Sampaio MJAM, Doberainer J (1984) A fourth \u003cem\u003eAzospirillum\u003c/em\u003e species from cereal roots. An Acad Bras Cienc 56:365\u003c/li\u003e\n\u003cli\u003eBalsanelli E, Tadra-Sfeir MZ, Faoro H, et al (2016) Molecular adaptations of \u003cem\u003eHerbaspirillum seropedicae\u003c/em\u003e during colonization of the maize rhizosphere. Environ Microbiol 18:2343\u0026ndash;2356. https://doi.org/10.1111/1462-2920.12887\u003c/li\u003e\n\u003cli\u003eBeracochea M, Taul\u0026eacute; C, Battistoni F (2019) Draft Genome Sequence of \u003cem\u003eKosakonia radicincitans\u003c/em\u003e UYSO10, an Endophytic Plant Growth-Promoting Bacterium of Sugarcane (\u003cem\u003eSaccharum officinarum\u003c/em\u003e. Microbiol Resour Announc 8:e01000-19. https://doi.org/10.1128/MRA.01000-19\u003c/li\u003e\n\u003cli\u003eBerg G, Rybakova D, Fischer D, et al (2020) Microbiome definition re-visited: old concepts and new challenges. Microbiome 8:1\u0026ndash;22. https://doi.org/10.1186/s40168-020-00875-0\u003c/li\u003e\n\u003cli\u003eBoller T, Felix G (2009) A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors. Annu Rev Plant Biol 60:379\u0026ndash;406. https://doi.org/10.1146/annurev.arplant.57.032905.105346\u003c/li\u003e\n\u003cli\u003eBradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u0026ndash;254. https://doi.org/10.1016/0003-2697(76)90527-3\u003c/li\u003e\n\u003cli\u003eCanarini A, Kaiser C, Merchant A, et al (2019) Root exudation of primary metabolites: Mechanisms and their roles in plant responses to environmental stimuli. Front Plant Sci 10:157. https://doi.org/10.3389/fpls.2019.00157\u003c/li\u003e\n\u003cli\u003eCarvalho PC, Lima DB, Leprevost, Felipe V Santos, Marlon D.M. Fischer, Juliana S.G. Aquino PF, et al (2016) Integrated analysis of shotgun proteomic data with PatternLab for proteomics 4.0. Nat Protoc 11:102\u0026ndash;117\u003c/li\u003e\n\u003cli\u003eCheng Z, Duan J, Hao Y, et al (2009) Identification of Bacterial Proteins Mediating the Interactions Between \u003cem\u003ePseudomonas putida\u003c/em\u003e UW4 and \u003cem\u003eBrassica napus\u003c/em\u003e (Canola). Mol Plant-Microbe Interact 22:686\u0026ndash;694. https://doi.org/10.1094/MPMI-22-6-0686\u003c/li\u003e\n\u003cli\u003eCompant S, Cambon MC, Vacher C, et al (2021) The plant endosphere world \u0026ndash; bacterial life within plants. Environ Microbiol 23:1812\u0026ndash;1829. https://doi.org/10.1111/1462-2920.15240\u003c/li\u003e\n\u003cli\u003eCordeiro FA, Tadra-Sfeir MZ, Huergo LF, et al (2013) Proteomic analysis of \u003cem\u003eHerbaspirillum seropedicae\u003c/em\u003e cultivated in the presence of sugar cane extract. J Proteome Res 12:1142\u0026ndash;1150. https://doi.org/10.1021/pr300746j\u003c/li\u003e\n\u003cli\u003eCoskun D, Britto DT, Shi W, Kronzucker HJ (2017) How plant root exudates shape the nitrogen cycle. Trends Plant Sci 22:661\u0026ndash;673. https://doi.org/10.1016/j.tplants.2017.05.004\u003c/li\u003e\n\u003cli\u003ede Bruijn FJ (2015) Biological nitrogen fixation. In: Lugtenberg B (ed) Principles of Plant-Microbe Interactions. Microbes for sustainable agriculture. Springer, pp 215\u0026ndash;224\u003c/li\u003e\n\u003cli\u003eDos Santos MF, Muniz de Padua VL, Nogueira EDM, et al (2010) Proteome of \u003cem\u003eGluconacetobacter diazotrophicus\u003c/em\u003e co-cultivated with sugarcane plantlets. J Proteomics 73:917\u0026ndash;931. https://doi.org/https://doi.org/10.1016/j.jprot.2009.12.005\u003c/li\u003e\n\u003cli\u003eHaichar F el Z, Santaella C, Heulin T, Achouak W (2014) Root exudates mediated interactions belowground. Soil Biol Biochem 77:69\u0026ndash;80. https://doi.org/10.1016/J.SOILBIO.2014.06.017\u003c/li\u003e\n\u003cli\u003eHan L, Zhang H, Bai X, Jiang B (2023) The peanut root exudate increases the transport and metabolism of nutrients and enhances the plant growth-promoting effects of \u003cem\u003eBurkholderia pyrrocinia\u003c/em\u003e strain P10. BMC Microbiol 23:1\u0026ndash;16. https://doi.org/10.1186/s12866-023-02818-9\u003c/li\u003e\n\u003cli\u003eHassani MA, Dur\u0026aacute;n P, Hacquard S (2018) Microbial interactions within the plant holobiont. Microbiome 6:58. https://doi.org/10.1186/s40168-018-0445-0\u003c/li\u003e\n\u003cli\u003eKandel S, Joubert P, Doty S (2017) Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms 5:77. https://doi.org/10.3390/microorganisms5040077\u003c/li\u003e\n\u003cli\u003eLeandro M, Andrade L, Vespoli L, et al (2021) Comparative proteomics reveals essential mechanisms for osmotolerance in \u003cem\u003eGluconacetobacter diazotrophicus\u003c/em\u003e. Res Microbiol 172:103785. https://doi.org/10.1016/j.resmic.2020.09.005\u003c/li\u003e\n\u003cli\u003eLery LMS, Hemerly AS, Nogueira EM, et al (2011) Quantitative proteomic analysis of the interaction between the endophytic plant-growth-promoting bacterium \u003cem\u003eGluconacetobacter diazotro\u003c/em\u003ephicus and sugarcane. Mol plant-microbe Interact 24:562\u0026ndash;576\u003c/li\u003e\n\u003cli\u003eLima A, Leyva A, Rivera B, et al (2021) Proteome remodeling in the \u003cem\u003eMycobacterium tuberculosis\u003c/em\u003e PknG knockout: molecular evidence for the role of this kinase in cell envelope biogenesis and hypoxia response. J Proteomics 244:104276. https://doi.org/https://doi.org/10.1016/j.jprot.2021.104276\u003c/li\u003e\n\u003cli\u003eLiu Y, Xu Z, Chen L, et al (2024) Root colonization by beneficial rhizobacteria. FEMS Microbiol Rev 48:1\u0026ndash;20. https://doi.org/10.1093/femsre/fuad066\u003c/li\u003e\n\u003cli\u003eMark GL, Dow JM, Kiely PD, et al (2005) Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. Proc Natl Acad Sci 102:17454\u0026ndash;17459. https://doi.org/10.1073/pnas.0506407102\u003c/li\u003e\n\u003cli\u003eMin KB, Yoon SS (2020) Transcriptome analysis reveals that the RNA polymerase-binding protein DksA1 has pleiotropic functions in \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e. J Biol Chem 295:3851\u0026ndash;3864. https://doi.org/10.1074/jbc.RA119.011692\u003c/li\u003e\n\u003cli\u003eMongkolsuk S, Whangsuk W, Vattanaviboon P, et al (2000) A \u003cem\u003eXanthomonas\u003c/em\u003e alkyl hydroperoxide reductase subunit C (\u003cem\u003eahpC\u003c/em\u003e) mutant showed an altered peroxide stress response and complex regulation of the compensatory response of peroxide detoxification enzymes. J Bacteriol 182:6845\u0026ndash;6849. https://doi.org/10.1128/JB.182.23.6845-6849.2000\u003c/li\u003e\n\u003cli\u003eNachin L, Nannmark U, Nystr\u0026ouml;m T, Nystro T (2005) Differential roles of the universal stress proteins of \u003cem\u003eEscherichia coli\u003c/em\u003e in oxidative stress resistance, adhesion, and Motility. J Bacteriol 187:6265\u0026ndash;6272. https://doi.org/10.1128/JB.187.18.6265\u003c/li\u003e\n\u003cli\u003eOgawa J, Long SR (1995) The \u003cem\u003eRhizobium meliloti\u003c/em\u003e groELc locus is required for regulation of early \u003cem\u003enod\u003c/em\u003e genes by the transcription activator NodD. Genes Dev 9:714\u0026ndash;729. https://doi.org/10.1101/gad.9.6.714\u003c/li\u003e\n\u003cli\u003eOrme\u0026ntilde;o-Orrillo E, Menna P, Almeida LGP, et al (2012) Genomic basis of broad host range and environmental adaptability of \u003cem\u003eRhizobium tropici\u003c/em\u003e CIAT 899 and \u003cem\u003eRhizobium\u003c/em\u003e sp. PRF 81 which are used in inoculants for common bean (\u003cem\u003ePhaseolus vulgaris\u003c/em\u003e L.). BMC Genomics 13:735. https://doi.org/10.1186/1471-2164-13-735\u003c/li\u003e\n\u003cli\u003ePankievicz VCS, Camilios-Neto D, Bonato P, et al (2016) RNA-seq transcriptional profiling of \u003cem\u003eHerbaspirillum seropedicae\u003c/em\u003e colonizing wheat (\u003cem\u003eTriticum aestivum\u003c/em\u003e) roots. Plant Mol Biol 90:589\u0026ndash;603. https://doi.org/10.1007/s11103-016-0430-6\u003c/li\u003e\n\u003cli\u003ePaungfoo-Lonhienne C, Lonhienne TGA, Yeoh YK, et al (2016) Crosstalk between sugarcane and a plant-growth promoting \u003cem\u003eBurkholderia\u003c/em\u003e species. Sci Rep 6:37389. DOI: 10.1038/srep37389\u003c/li\u003e\n\u003cli\u003ePohl CH, Kock JLF (2014) Oxidized fatty acids as inter-kingdom signaling molecules. Molecules 19:1273\u0026ndash;1285. https://doi.org/10.3390/molecules19011273\u003c/li\u003e\n\u003cli\u003eReis VM, Olivares FL, de Oliveira ALM, et al (1999) Technical approaches to inoculate micropropagated sugar cane plants were \u003cem\u003eAcetobacter diazotrophicus\u003c/em\u003e. Plant Soil 206:205\u0026ndash;211. https://doi.org/10.1023/A:1004436611397\u003c/li\u003e\n\u003cli\u003eRosenberg E, ZIlber-Rosenberg I (2016) Microbes Drive Evolution of Animals and Plants: the Hologenome Concept. MBio 7:e01395. https://doi.org/doi: 10.1128/mBio.01395-15\u003c/li\u003e\n\u003cli\u003eSabri S, Nielsen LK, Vickers CE (2013) Molecular control of sucrose utilization in \u003cem\u003eEscherichia coli\u003c/em\u003e W, an efficient sucrose-utilizing strain. Appl Environ Microbiol 79:478\u0026ndash;487. https://doi.org/10.1128/AEM.02544-12\u003c/li\u003e\n\u003cli\u003eSheibani-Tezerji R, Rattei T, Sessitsch A, et al (2015) Transcriptome profiling of the endophyte \u003cem\u003eBurkholderia phytofirmans\u003c/em\u003e psjn indicates sensing of the plant environment and drought stress. MBio 6:e00621-15. https://doi.org/10.1128/mBio.00621-15\u003c/li\u003e\n\u003cli\u003eShidore T, Dinse T, Ohrlein J, et al (2012) Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte \u003cem\u003eAzoarcus\u003c/em\u003e sp. strain BH72. Environ Microbiol 14:2775\u0026ndash;2787. https://doi.org/10.1111/j.1462-2920.2012.02777.x\u003c/li\u003e\n\u003cli\u003eTaul\u0026eacute; C, Castillo A, Villar S, et al (2016) Endophytic colonization of sugarcane (\u003cem\u003eSaccharum officinarum\u003c/em\u003e) by the novel diazotrophs \u003cem\u003eShinella\u003c/em\u003e sp. UYSO24 and \u003cem\u003eEnterobacter\u003c/em\u003e sp. UYSO10. Plant Soil 403:403\u0026ndash;418. https://doi.org/https://doi.org/10.1007/s11104-016-2813-5\u003c/li\u003e\n\u003cli\u003eTaul\u0026eacute; C, Luizzi H, Beracochea M, et al (2019) The Mo- and Fe-nitrogenases of the endophyte \u003cem\u003eKosakonia\u003c/em\u003e sp. UYSO10 are necessary for growth promotion of sugarcane. Ann Microbiol 69:741\u0026ndash;750. https://doi.org/https://doi.org/10.1007/s13213-019-01466-7\u003c/li\u003e\n\u003cli\u003eTaul\u0026eacute; C, Mareque C, Barlocco C, et al (2012) The contribution of nitrogen fixation to sugarcane (\u003cem\u003eSaccharum officinarum\u003c/em\u003e L.), and the identification and characterization of part of the associated diazotrophic bacterial community. Plant Soil 356:35\u0026ndash;49. https://doi.org/10.1007/s11104-011-1023-4\u003c/li\u003e\n\u003cli\u003eTaul\u0026eacute; C, Vaz-Jauri P, Battistoni F (2021) Insights into the early stages of plant\u0026ndash;endophytic bacteria interaction. World J Microbiol Biotechnol 37:1\u0026ndash;9. https://doi.org/10.1007/s11274-020-02966-4\u003c/li\u003e\n\u003cli\u003eTejera N, Ortega E, Rodes R, Lluch C (2006) Nitrogen compounds in the apoplastic sap of sugarcane stem: some implications in the association with endophytes. J Plant Physiol 163:80\u0026ndash;85. https://doi.org/10.1016/j.jplph.2005.03.010\u003c/li\u003e\n\u003cli\u003eUranga C, Beld J, Mrse A, et al (2016) Fatty acid esters produced by \u003cem\u003eLasiodiplodia theobromae\u003c/em\u003e function as growth regulators in tobacco seedlings. Biochem Biophys Res Commun 472:339\u0026ndash;345. https://doi.org/10.1016/j.bbrc.2016.02.104\u003c/li\u003e\n\u003cli\u003eVives-Peris V, de Ollas C, G\u0026oacute;mez-Cadenas A, P\u0026eacute;rez-Clemente RM (2020) Root exudates: from plant to rhizosphere and beyond. Plant Cell Rep 39:3\u0026ndash;17. https://doi.org/10.1007/s00299-019-02447-5\u003c/li\u003e\n\u003cli\u003eWitzel K, Strehmel N, Baldermann S, et al (2017) \u003cem\u003eArabidopsis thaliana\u003c/em\u003e root and root exudate metabolism is altered by the growth-promoting bacterium \u003cem\u003eKosakonia radicincitans\u003c/em\u003e DSM 16656T. Plant Soil 419:557\u0026ndash;573. https://doi.org/10.1007/s11104-017-3371-1\u003c/li\u003e\n\u003cli\u003eXie Z, Ulrich LE, Zhulin IB, Alexandre G (2010) PAS domain containing chemoreceptor couples dynamic changes in metabolism with chemotaxis. PNAS 107:2235\u0026ndash;2240. https://doi.org/https://doi.org/10.1073/pnas.0910055107\u003c/li\u003e\n\u003cli\u003eYao J, Allen C (2007) The plant pathogen Ralstonia solanacearum needs aerotaxis for normal biofilm formation and interactions with its tomato host. J Bacteriol 189:6415\u0026ndash;6424. https://doi.org/10.1128/JB.00398-07\u003c/li\u003e\n\u003cli\u003eZimmer DP, Soupene E, Lee HL, et al (2000) Nitrogen regulatory protein C-controlled genes of \u003cem\u003eEscherichia coli\u003c/em\u003e: scavenging as a defense against nitrogen limitation. Proc Natl Acad Sci U S A 97:14674\u0026ndash;14679. https://doi.org/10.1073/pnas.97.26.14674\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003eTable 1. Identity and general features of the spots with differential expression detected by DIGE, in the proteome of the strain UYSO10 grown in the presence of sugarcane root exudates.\u0026nbsp;\u003c/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"926\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eFold change\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003ep value\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eEncoding\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e\u0026nbsp;gene in UYSO10\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMascot score\u003csup\u003ea\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSequence coverage\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eNumber of peptide asigned\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMass (kDa)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003ePI\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eIdentified protein\u003csup\u003eb\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eCOG category\u003csup\u003ec\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eCelular localization\u003csup\u003ed\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"29.37365010799136%\" colspan=\"4\" valign=\"bottom\"\u003e\n \u003cp\u003e\u003cstrong\u003eUp-regulated in the presence of root exudates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.8034557235421165%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.559395248380129%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.291576673866091%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.8876889848812093%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.56155507559395%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.34341252699784%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.1792656587473%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e2.51\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.01\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0935\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e89\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e14\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e52.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e5.06\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eAspartate ammonia-lyase (EC 4.3.1.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eC\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e2.21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.00037\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3581\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e243\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e17\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e46.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e6.28\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eUrea ABC transporter, substrate binding protein UrtA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.55\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.012\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3581\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e233\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e24\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e46.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e6.28\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eUrea ABC transporter, substrate binding protein UrtA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.45\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.045\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3581\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e102\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e11\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e46.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e6.28\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eUrea ABC transporter, substrate binding protein UrtA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.45\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.04\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0469\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e65\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e21\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e41.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e5.08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003ePhosphoglycerate kinase (EC 2.7.2.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.89\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.022\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3373\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e324\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e47\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e25.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e4.27\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eUncharacterized protein conserved in bacteria\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eE\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.028\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2573\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e91\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e14\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e45,9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e7.08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eGlucose-1-phosphatase (EC 3.1.3.10)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.55\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.042\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2573\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e75\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e9\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e45.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e7.08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eGlucose-1-phosphatase (EC 3.1.3.10)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.177105831533474%\" colspan=\"5\"\u003e\n \u003cp\u003e\u003cstrong\u003eDown-regulated in the presence of root exudates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.559395248380129%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.291576673866091%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.8876889848812093%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.56155507559395%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.34341252699784%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.1792656587473%\" valign=\"bottom\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.69\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.0063\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0266\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e171\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e24\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e32.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e8.99\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eMaltose operon periplasmic protein MalM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.016\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5888\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e454\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e65\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e30.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e6.58\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eRibose ABC transporter system, periplasmic ribose-binding protein Rbs (TC 3.A.1.2.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.77\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.0079\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2505\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e47\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e7\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e24.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e4.35\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eFIG00955836: hypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eJ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eE\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.58\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.0075\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2413\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e277\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e41\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e37.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e6.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane protein A precursor\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eOM\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.37\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.025\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2413\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e549\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e54\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e37.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e6.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane protein A precursor\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eOM\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.37\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.039\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0940\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e164\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e25\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e57.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e4.85\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eHeat shock protein 60 family chaperone GroEL\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.37\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.039\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1105\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e84\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e22\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e23.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e5.06\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eFIG00975563: hypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e1.49\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.93311758360302%\" valign=\"top\"\u003e\n \u003cp\u003e0.019\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.218985976267529%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3704\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.148867313915858%\" valign=\"top\"\u003e\n \u003cp\u003e96\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.796116504854369%\" valign=\"top\"\u003e\n \u003cp\u003e14\u0026nbsp;%\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.5512405609492985%\" valign=\"top\"\u003e\n \u003cp\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"5.28586839266451%\" valign=\"top\"\u003e\n \u003cp\u003e63.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"3.883495145631068%\" valign=\"top\"\u003e\n \u003cp\u003e5.78\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.528586839266453%\" valign=\"top\"\u003e\n \u003cp\u003eTrehalase (EC 3.2.1.28)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"7.335490830636462%\" valign=\"top\"\u003e\n \u003cp\u003eT\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.169363538295578%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003ea\u003c/sup\u003eAll Mascot scores reported were statistically significant (p \u0026lt;0.05). \u003csup\u003eb\u003c/sup\u003eThe annotation of the proteins was obtained using the genome of the strain UYSO10. \u003csup\u003ec\u003c/sup\u003eCOG categories: J- translation; O- chaperone molecules and related functions; M- structure and biogenesis of the outer membrane; T- signal transduction; G- carbohydrate transport and metabolism; E- transport and metabolism of amino acids; S- no functional prediction. \u003csup\u003ed\u003c/sup\u003eThe results showed correspond to prediction using PSORTb algorithm, and when no output was obtained, Cello algorithm was applied and the result reported is indicated with a \u0026quot;*\u0026quot;. OM- Outer membrane, C- Cytoplasmic, P- Periplasmic, E- Extracellular.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTable 2. Proteins exclusively identified in the samples of the strain UYSO10 exposed to sugarcane root exudates, detected by the shotgun approach.\u0026nbsp;\u003c/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"969\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eGene in UYSO10\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eIdentified protein\u003csup\u003ea\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eCOG category\u003csup\u003eb\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e# replicates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSpectrum counts\u003csup\u003ec\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eLocalization\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"71.9298245614035%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eUnique in the presence of root exudates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0953\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eFumarate reductase iron-sulfur protein (EC 1.3.5.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e24\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/P*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1506\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eRNA polymerase-binding transcription factor DksA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0266\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eMaltose operon periplasmic protein MalM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3373\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eUncharacterized protein conserved in bacteria\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e23\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"71.9298245614035%\" colspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eUnique in the\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003eabsence\u0026nbsp;of root exudates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5355\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eSuccinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e48\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/C*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2947\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eFumarate hydratase class II (EC 4.2.1.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e35\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4156\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eD-lactate dehydrogenase (EC 1.1.1.28)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e27\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/C*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4140\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eBifunctional protein: zinc-containing alcohol dehydrogenase\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4591\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eCytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3055\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eMethionine synthase II (cobalamin-independent)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e41\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5669\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eDipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e24\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/C*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1401\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0105\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e19\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0251\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eAspartokinase (EC 2.7.2.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e19\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5671\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eDipeptide transport system permease protein DppB (TC 3.A.1.5.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2314\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ePutrescine transport ATP-binding protein PotG (TC 3.A.1.11.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2235\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eGlutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0233\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eIMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eF\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e31\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1623\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eXanthine-guanine phosphoribosyltransferase (EC 2.4.2.22)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eF\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4191\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e1-phosphofructokinase (EC 2.7.1.56)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e82\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5520\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eLimit dextrin alpha-1,6-maltotetraose-hydrolase (EC 3.2.1.196)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e60\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1103\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eDihydroxyacetone kinase, ATP-dependent (EC 2.7.1.29)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e45\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0357\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eRibose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0834\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eFructokinase (EC 2.7.1.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/C*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1616\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eAcyl-CoA dehydrogenase\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e45\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5362\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eRibonuclease G\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eJ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e29\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0499\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ePeptide chain release factor 2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eJ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e19\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3460\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;TsaC/YrdC paralog\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eJ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1783\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eROK family sugar kinase or transcriptional regulator\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eK\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e31\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5275\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ePenicillin-binding protein activator LpoA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eL\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0281\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eReplicative DNA helicase (DnaB) (EC 3.6.4.12)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eL\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0226\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eNADH pyrophosphatase (EC 3.6.1.22)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eL\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2683\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eTranscription-repair coupling factor\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eL\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e29\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3519\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e31\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4804\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eTranslation elongation factor LepA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/C*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4044\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eProtein YeeZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4627\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane porin for chitooligosaccharides ChiP\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eME*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0778\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eMembrane-bound lytic murein transglycosylase B\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/P*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2237\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eApolipoprotein N-acyltransferase (EC 2.3.1.-)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1030\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eMethyl-accepting chemotaxis sensor/transducer protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e72\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3724\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eTsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5397\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eTrk potassium uptake system protein TrkA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e31\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/C*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0721\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eNitrate ABC transporter, permease protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1733\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ePeriplasmic hemin-binding protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2405\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eBis-ABC ATPase Uup\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e36\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5852\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eChromate reductase (EC 1.6.5.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e36\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2150\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e5-methylthioribose kinase (EC 2.7.1.100)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0341\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eAcetate permease ActP (cation/acetate symporter)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e24\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3617\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eHydrolase, alpha/beta fold family\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eP*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5883\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eATPase RavA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1220\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eMultimeric flavodoxin WrbA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0640\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eHypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e50\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5767\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eUncharacterized protein YicH\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e31\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3147\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ehypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC/ME*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5622\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ehypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4435\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eFIG00626109: hypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2446\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003e2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5361\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eUncharacterized protein YhdP\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0239\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eIsocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eT\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0208\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eProtein translocase subunit SecE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eU\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3136\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eBeta-lactamase (EC 3.5.2.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eV\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eP/ME*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2773\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003ehypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eV\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e19\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"10.010319917440661%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2374\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"61.919504643962846%\" valign=\"top\"\u003e\n \u003cp\u003eLipid A export permease/ATP-binding protein MsbA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.501547987616099%\" valign=\"top\"\u003e\n \u003cp\u003eV\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.604747162022703%\" valign=\"top\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"6.707946336429308%\" valign=\"top\"\u003e\n \u003cp\u003e15\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.25593395252838%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003ea\u003c/sup\u003eThe protein annotation is obtained from the search using the strain UYSO10 genome database. \u003csup\u003eb\u003c/sup\u003eCOG categories: J- translation; L- replication, recombination and repair; K- transcription; O- chaperone molecules and related functions; M- structure and biogenesis of the cell wall and the outer membrane; N- secretion, mobility and chemotaxis; T-signal transduction; P-transport and metabolism of inorganic ions; C-energy production and conversion; G-transport and carbohydrate metabolism; E-transport and metabolism of amino acids; F-transport and nucleotide metabolism; U- intracellular traffic and secretion; V- defense mechanism; I-lipid metabolism; R-general functional prediction; S-no functional prediction. \u003csup\u003ec\u003c/sup\u003eThe total signal corresponds to the sum of the number of spectra assigned to each protein in each replica. It is considered a measure of their relative abundance. \u003csup\u003ed\u003c/sup\u003eThe results showed correspond to prediction using PSORTb algorithm, and when no output was obtained, Cello algorithm was applied and the result reported is indicated with a \u0026quot;*\u0026quot;. OM- Outer membrane, CM- Cytoplasmic membrane, C- Cytoplasmic, P- Periplasmic, E- Extracellular, I- Indeterminate.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTable 3. Proteins with statistical differential relative abundance of the UYSO10 strain exposed to root exudates, identified by the shotgun approach.\u0026nbsp;\u003c/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"947\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eFold change\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003ep value\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eGene in UYSO10\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eIdentified protein\u003csup\u003ea\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eCOG category\u003csup\u003eb\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eLocalization\u003csup\u003ec\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"82.5765575501584%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eIncreased relative abundance\u0026nbsp;in the presence of root exudates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.79\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.00008\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5873\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eATP synthase F0 sector subunit b (EC 3.6.3.14)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0004\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4596\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eSuccinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.67\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0081\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0935\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAspartate ammonia-lyase (EC 4.3.1.1)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.87\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0013\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1052\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eFructose-1,6-bisphosphatase, type I (EC 3.1.3.11)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.75\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.00001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1546\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAcetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.24\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0035\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5421\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eLSU ribosomal protein L16p (L10e)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eJ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0105\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2391\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane porin OmpF\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eME\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0121\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1538\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane chaperone Skp (OmpH) precursor @ Outer membrane protein H precursor\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.09\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0106\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3810\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eChemotaxis response - phosphatase CheZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e4.20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0123\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5222\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAerotaxis sensor receptor protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.50\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0004\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1850\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eCell division trigger factor (EC 5.2.1.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0032\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2197\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAlkyl hydroperoxide reductase protein C (EC 1.11.1.15)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.42\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0259\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0939\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eHeat shock protein 60 family co-chaperone GroES\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.86\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0136\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2199\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eUniversal stress protein G\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eT\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"82.5765575501584%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eDecreased relative abundance \u0026nbsp;in the presence of root exudates\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.80\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0246\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2907\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eN-ethylmaleimide reductase (EC 1.-.-.-)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.02\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2694\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eSuccinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.89\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0058\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3648\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAldehyde dehydrogenase (EC 1.2.1.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.73\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0229\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3584\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAllophanate hydrolase (EC 3.5.1.54)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.46\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0441\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3583\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eUrea carboxylase (EC 6.3.4.6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.75\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0422\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3581\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eUrea ABC transporter, substrate binding protein UrtA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.00\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0043\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3416\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003ePeriplasmic Murein Peptide-Binding Protein MppA\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.93\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0006\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3304\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eABC transporter periplasmic-binding protein YdcS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.63\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0244\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5340\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eGlutamate synthase [NADPH] small chain (EC 1.4.1.13)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0097\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3047\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eDipeptidyl carboxypeptidase Dcp (EC 3.4.15.5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.69\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0013\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5628\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOligopeptidase A (EC 3.4.24.70)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eE\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.26\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0089\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2938\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eAdenosine deaminase (EC 3.5.4.4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eF\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.23\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0044\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5518\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eGlucose-1-phosphate adenylyltransferase (EC 2.7.7.27)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.89\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0175\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3704\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eTrehalase (EC 3.2.1.28) @ Periplasmic trehalase (EC 3.2.1.28)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.42\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0374\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0252\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eGlucose-6-phosphate isomerase (EC 5.3.1.9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.39\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0094\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4192\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eBifunctional PTS system fructose-specific transporter subunit IIA/HPr protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eG\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e4.30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0241\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0119\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane vitamin B12 receptor BtuB\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eH\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eME\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e5.51\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0102\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_0197\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eEnoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.65\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0014\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4361\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eLong-chain fatty acid transport protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eI\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eME\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e5.29\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.00001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4543\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eExcinuclease ABC subunit B\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eL\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.58\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0028\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3747\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eTail-specific protease precursor (EC 3.4.21.102)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.02\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0045\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1422\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eME\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.98\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0034\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4265\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane porin OmpC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eME\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.78\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.002\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1537\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane protein assembly factor YaeT\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eM\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eME\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.64\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0019\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3853\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eFlagellar M-ring protein FliF\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC/ME*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.26\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.003\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3514\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eMethyl-accepting chemotaxis protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.33\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0378\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3814\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eMethyl-accepting chemotaxis protein IV (dipeptide chemoreceptor protein)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.02\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0007\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2027\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eMethyl-accepting chemotaxis sensor/transducer protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eN\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eMC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e6.22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0018\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_5347\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eOuter membrane stress sensor protease DegQ, serine protease\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.26\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0278\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1856\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003ePeptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.43\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0131\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2599\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eFerrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e3.43\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0233\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_3369\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eUncharacterized protein YdcJ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.67\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0211\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_1105\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eFIG00975563: hypothetical protein\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP*\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.38\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0001\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2026\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003edomain of unknown function DUF1745\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eS\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e1.70\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0018\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_2023\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eresponse regulator receiver modulated metal dependent phosphohydrolase\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eT\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eC\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e2.35\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003e0.0109\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.143611404435058%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eUYSO10_4584\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"53.537486800422386%\" valign=\"top\"\u003e\n \u003cp\u003eTol-Pal system beta propeller repeat protein TolB\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.447729672650475%\" valign=\"top\"\u003e\n \u003cp\u003eU\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"8.97571277719113%\" valign=\"top\"\u003e\n \u003cp\u003eP\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003ea\u003c/sup\u003eThe protein annotation is obtained from the search using the strain UYSO10 database. \u003csup\u003eb\u003c/sup\u003eCOG categories: J-translation; L- replication, recombination and repair; O- chaperone molecules and related functions; M- structure and biogenesis of the cell envelope and outer membrane; N- secretion, mobility and chemotaxis; T- signal transduction; P- transport and metabolism of inorganic ions; C- energy production and conversion; G- carbohydrate transport and metabolism; E- transport and metabolism of amino acids; F- nucleotide transport and metabolism; H- coenzymatic metabolism; I- lipid metabolism; U- intracellular traffic and secretion; S- no functional prediction. \u003csup\u003ec\u003c/sup\u003eThe results showed correspond to prediction using PSORTb algorithm, and when no output was obtained, Cello algorithm was applied and the result reported is indicated with a \u0026quot;*\u0026quot;. OM- Outer membrane, CM- Cytoplasmic membrane, C- Cytoplasmic, P- Periplasmic.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"plant-and-soil","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"plso","sideBox":"Learn more about [Plant and Soil](https://www.springer.com/journal/11104)","snPcode":"11104","submissionUrl":"https://submission.nature.com/new-submission/11104/3","title":"Plant and Soil","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Kosakonia radicincitans, sugarcane, exudates, proteomics","lastPublishedDoi":"10.21203/rs.3.rs-4034332/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4034332/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cem\u003eBackgroun\u003c/em\u003e: Plant-associated microbiotas play a key role in plant health, growth, and stresses resilience. These microbiotas are considered the second plant genome by expanding its genetic potential. One of the main components of the plant microbiota is the endophytic bacterial communities, which live in the plant's internal tissues. The main sources of this microbiota are the seed and the soil where the plant grows. Particularly, soil bacteria are attracted by different signals present in plant root exudates, to later colonize the rhizoplane and infect internal tissues. Although the colonization and infection processes of endophytic bacteria are well documented, the molecular bases of the mechanisms involved in the plant-endophyte interaction are still poorly understood. Previously it was shown that strain \u003cem\u003eKosakonia radicincitans\u003c/em\u003e UYSO10 promotes the growth of sugarcane plants and was defined as a true endophyte. Moreover, it was demonstrated that the biological nitrogen fixation process is involved in the plant growth promotion observed. The \u003cem\u003eaim\u003c/em\u003e of this work is to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the diazotrophic endophytic strain UYSO10 and sugarcane plants.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eMethodology\u003c/em\u003e: a proteomic approach was conducted in the strain UYSO10 exposed or not to sugarcane exudates.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eResults\u003c/em\u003e showed that in the presence of root exudates, strain UYSO10 senses the environment and adapts its proteome to transport and metabolize different nutrients, and to interact with the host plant. These results deepen the knowledge of the potential mechanisms involved in the early stage of plant-bacteria endophyte interaction.\u003c/p\u003e","manuscriptTitle":"Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-13 08:18:03","doi":"10.21203/rs.3.rs-4034332/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revisions","date":"2024-04-28T16:33:36+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2024-03-13T00:39:49+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-03-11T12:06:44+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"Plant and Soil","date":"2024-03-10T07:46:19+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-03-10T07:43:27+00:00","index":"","fulltext":""},{"type":"submitted","content":"Plant and Soil","date":"2024-03-07T20:27:17+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"plant-and-soil","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"plso","sideBox":"Learn more about [Plant and Soil](https://www.springer.com/journal/11104)","snPcode":"11104","submissionUrl":"https://submission.nature.com/new-submission/11104/3","title":"Plant and Soil","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"c54f0311-8e13-42ce-88d1-02f99c182853","owner":[],"postedDate":"March 13th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-12-09T16:04:19+00:00","versionOfRecord":{"articleIdentity":"rs-4034332","link":"https://doi.org/10.1007/s11104-024-07112-9","journal":{"identity":"plant-and-soil","isVorOnly":false,"title":"Plant and Soil"},"publishedOn":"2024-12-03 15:57:48","publishedOnDateReadable":"December 3rd, 2024"},"versionCreatedAt":"2024-03-13 08:18:03","video":"","vorDoi":"10.1007/s11104-024-07112-9","vorDoiUrl":"https://doi.org/10.1007/s11104-024-07112-9","workflowStages":[]},"version":"v1","identity":"rs-4034332","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4034332","identity":"rs-4034332","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}