Expression of plastin in the eutopic endometrium and ectopic foci of endometriosis

Objective To explore the candidate gene and its function in the eutopic endometrium of endometriosis;by the measurement of the expression of plastin(T-plastin and L-plastin)in endometrium,investigate its function in the development and progression of endometriosis.Methods Our group has screened the expression of differential genes in patients with endometriosis.Ⅰ searched the relative nucleotide in nucleotide database with the help of BLASTN(Nucleotide-nucleotide BLAST).After BLAST search,the gene with the highest homology was chosen.The first chosen gene was BC039049,GI:25058020 coding protein:homo plastin 3(T-plastin),mRNA(cDNA clone MGC:4910 IMAGE:3446726),and the second one was:BC022364,GI:18490212 coding protein:homo plastin 2(L-plastin),mRNA(cDNA clone MGC:23890 IMAGE:4730117).The eutopic endometria and ectopic endometria of 36 cases,which were pathologically proved endometriosis,were chosen as experimental group,and the eutopic endometria of 22 cases without endometriosis as control group.The expression of plastin-2 and plastin-3 mRNA was detected by hybridization in situ.The plastin expression in the proliferative phase was compared with the counterparts in the secretive phase among the study groups.Results The expression level of T-plastin in the ectopic endometria was significantly lower than those in control group and the eutopic endometria of patients.(P0.01).The expression level of L-plastin in the eutopic endometria and the ectopic endometria of patients were significantly greater than those in control group(P0.01).Only the statistically significant difference between L-plastin expression levels in the proliferative phase and the counterparts in secretive phase was found in the eutopic endometria(P0.05).Conclusion The unusually elevated levels of L-plastin may play an important role in the development and progression of endometriosis.