Endometrial epithelial cells with high ALDH activity control uterine development and regeneration

Suni Tang, Anna Catherine Unser, Peixin Jiang, Sydney E Parks, Genesis J Herrera, Ting Geng, Linda Alpuing-Radilla, Brooke A Thigpen, Xiaoming Guan, Diana Monsivais
In: eLife · 2026 · doi:10.7554/elife.110975 · W7160433785
article OA: gold CC0
📄 Open PDF View on OpenAlex View at publisher

Abstract

Adult stem cells are thought to drive the regenerative potential of the endometrium and contribute to the pathogenesis of endometriosis, however, their identity and defining features remain to be characterized. Here, we used in vivo and in vitro approaches to demonstrate that cells with high aldehyde dehydrogenase 1 activity (ALDHHI cells) were long lived progenitors in the endometrial epithelium with a higher organoid formation capacity, long-term passaging potential, and stemness gene signatures. Using lineage tracing with an Aldh1a1cre/ERT2; ROSA26tdTomato reporter mouse, Aldh1a1+ cells expanded during postnatal development, estrus cycling, and following post-partum repair. In response to ovariectomy or exogenous estradiol, we found that ALDH1A1+ cells localized to glandular crypts of the endometrium or throughout the luminal epithelium, respectively, indicating that their spatial localization is hormone sensitive. Functionally, we found that selective ablation of ALDH1A1+ cells in Aldh1a1cre/ERT2; ROSA26-DTRflox/flox mice decreased endometrial gland number and FOXA2 expression. These findings were recapitulated in the human endometrium, where endometrial epithelial organoids with high ALDH activity (ALDHHI cells) showed a higher organoid formation capacity than ALDHLO cells and displayed unique transcriptomes with fewer luminal-like ciliated cells. Overall, our studies indicate that ALDH1A1+ cells are hormone-sensitive adult stem cells in the endometrium with regenerative potential that are critical for endometrial development and function.

My notes (saved in your browser only)

Condition tags

endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (61)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK