A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics

A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics Raymond Thomas, Charles Manful, Thu Pham This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-713915/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 13 You are reading this latest preprint version Abstract Introduction Monoacetyldiglycerides (MAcDG), are acetylated triglycerides (TG) and an emerging class of bioactive or functional lipid with promising nutritional, medical, and industrial applications. A major challenge exists when analyzing MAcDG from other subclasses of TG in biological matrices, limiting knowledge on their applications and metabolism. Objective and Methods Here in we demonstrate a multimodal analytical method for resolution, identification and quantitation of MAcDG in biological samples based on thin layer chromatography-flame ionization detection complimentary with C30-reversed phase liquid chromatography-high resolution accurate mass tandem mass spectrometry. We further apply this method to determine the MAcDG molecular species composition and quantity in E. solidaginis larvae. Results and Conclusion These findings suggest that the proposed analytical method could simultaneously provide a fast, accurate, sensitive, high throughput analysis of MAcDG from other TG subclasses, including the fatty acids, isomers and molecular species composition. We believe this method will allow for MAcDG to be included during routine lipidomics analysis of biological samples and will have broad interests and applications in the scientific community in areas such as nutrition, climate change, medicine and biofuel innovations. Cardiac & Cardiovascular Systems Endocrinology & Metabolism Thin layer chromatography triglyceride analysis acylated triglyceride liquid chromatography mass spectrometry functional lipids Eurosta solidaginis Full Text Supplementary Files GraphicalAbstract.docx ManfuletalSupplementaryInformationMAcDGOct28.docx Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Major revision 29 Jul, 2021 Review # 2 received at journal 28 Jul, 2021 Review # 3 received at journal 27 Jul, 2021 Reviewer # 3 agreed at journal 24 Jul, 2021 Reviewer # 2 agreed at journal 22 Jul, 2021 Review # 1 received at journal 15 Jul, 2021 Reviews received at journal 14 Jul, 2021 Reviewers invited by journal 14 Jul, 2021 Editor assigned by journal 14 Jul, 2021 Reviewer # 1 agreed at journal 13 Jul, 2021 Submission checks completed at journal 13 Jul, 2021 Editor invited by journal 13 Jul, 2021 First submitted to journal 12 Jul, 2021 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-713915","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":39436730,"identity":"6e15ba66-d8d7-4e97-bfa8-44f61d4dfa48","order_by":0,"name":"Raymond 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major challenge exists when analyzing MAcDG from other subclasses of TG in biological matrices, limiting knowledge on their applications and metabolism. \u003c/p\u003e\u003cp\u003eObjective and Methods\u003c/p\u003e\u003cp\u003eHere in we demonstrate a multimodal analytical method for resolution, identification and quantitation of MAcDG in biological samples based on thin layer chromatography-flame ionization detection complimentary with C30-reversed phase liquid chromatography-high resolution accurate mass tandem mass spectrometry. 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