Based on Deep Eutectic Solvents Combined with Gas Chromatography Determination of γ-nonolactone in Coconut Juice

Determination of γ-nonolactone in coconut juice by deep eutectic solvents (DES) combined with gas chromatography was developed. Acetonitrile added deep eutectic solvents was used to extract γ-nonolactone from coconut juice with ultrasonic assist. The effects of different proportions of DES on the extraction efficiency of γ-nonolactone in coconut juice were investigated. The results showed that the extraction efficiency of γ-nonolactone in coconut juice with acetonitrile solution added with deep eutectic solvents was the highest, and the samples could be directly injected through membrane after high-speed refrigerated centrifugation. γ-nonolactone has a good linear relationship in the range of 0.1 ~ 20.0 µg/mL, the detection limit of the method is 0.2 mg/kg, the limit of quantification is 0.5 mg/kg, and the addition level is 0.2, 0.5, 10.0 mg/kg recovery the range is 81.5 ~ 105.0%,and the relative standard deviation (n = 6) is in the range of 0.6 ~ 5.8%. The method is simple to operate, has high sensitivity, and good precision. It can provide a reference for the analysis and detection of whether coconut water is added with γ-nonolactone .