Inonotus obliquus (Fr.) Pilat (IOPE) ameliorates immune response via LKB1- Bax-Casepase-3-PARP pathways in B16-F10 cells induced low immunity BALB/c mice model

Objective: Inonotus obliquus (Fr.) Pilat extract (IOPE) is a major active carbohydrate, an important herb used in traditional medicine, the aim of this study was to investigate the immunomodulatory effects and possible underlying mechanisms of IOPE in B16-F10 cells induced low immunity mice. Material: and Methods IOPE was extracted and prepared, the inhibitory effect on B16-F10 cells was determined in vitro, different concentrations of the IOPE (0.1, 0.2, 0.4, 0.8 mg/mL) were added to cells for 2, 12, 24, 48 h. BALB/c mice model was established by B16-F10 cells, and the IOPE (500, 250, 125 mg/kg/bw) were oral administration into mice. Results: : The IOPE could not only owned inhibitory effect on B16-F10 cells (0.8 mg/mL), but also had a potent immune response improvement (250 mg/kg/bw), and effectively improved spleen immune index, CD 3+ and CD 8+ cell expression (79.8%,20.1%), strengthened phagocytosis of macrophages, significantly inhibited the over expression of inflammatory factors IL-6(308.02±6.48 pg/mL), IL-8(381.84±8.93 pg/mL), TNF-α(1877.50±11.11 pg/mL), promoted the expression of anti-inflammatory factor IL-10 (419.90±5.55 pg/mL), finally activated the LKB1-Bax-Casepase-3-PARP pathway. Conclusions: : IOPE might be potentially used as an effective regulator for the immunity improvement in future.