Polyhydroxyalkanoate synthesis ability of the salt-tolerant bacterial strain Salinicola lusitanus BTNC26 isolated from coastal zone in Vietnam and its application in food packaging film production

Polyhydroxyalkanoate synthesis ability of the salt-tolerant bacterial strain Salinicola lusitanus BTNC26 isolated from coastal zone in Vietnam and its application in food packaging film production | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Polyhydroxyalkanoate synthesis ability of the salt-tolerant bacterial strain Salinicola lusitanus BTNC26 isolated from coastal zone in Vietnam and its application in food packaging film production THI TAM THU NGUYEN, LE THI NHI-CONG, THI HUYEN NGA TRAN, KIEN CUONG PHAM, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9265218/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Recently, polyhydroxyalkanoates (PHA) have garnered systematic attention as biodegradable alternatives to petrochemical plastics. This study aimed to identify bacterial strains synthesizing poly(3-hydroxybutyrate) (PHB) from Vietnam's coastal zones. Strain BTNC26, isolated as a PHA producer, was identified as Salinicola lusitanus BTNC26 via 16S rRNA gene sequencing (GenBank: PZ087081). Optimization of carbon (glucose) and nitrogen (yeast extract) sources, pH (7), temperature (30°C), and NaCl (5%) enhanced cell growth and PHA accumulation. Under these conditions, biopolymer yield reached 73.07% after 3 days. FTIR, GC–MS, and NMR confirmed PHB identity: FTIR showed a carbonyl peak at 1710.52 cm⁻¹; ¹H/¹³C NMR revealed resonances at 169.14 (C = O), 67.62 (–CH–), 40.80 (–CH₂–), and 19.76 ppm (–CH₃); GC–MS displayed the 3-hydroxybutyric acid methyl ester molecular ion. Scale-up fermentation (200 mL to 100 L) with 10 g/L glucose supplementation yielded PHB concentrations of 4.76, 4.23, 3.98, and 3.59 g/L at 200 mL, 1 L, 10 L, and 20 L, respectively. Composite films incorporating extracted PHB with PBAT, CaCO₃, PEG, and glycerol exhibited thin, strong, and flexible properties, with biodegradability confirmed in laboratory seawater assays. These findings highlight the potential of halophilic S. lusitanus for seawater-degradable PHB production, informing strain- and condition-specific strategies for industrial scalability. Food packaging film Polyhydroxyalkanoate Salinicola lusitanus Salt-tolerant bacterium Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9265218","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":625604305,"identity":"a2388870-37fa-43af-b7e1-266476283950","order_by":0,"name":"THI TAM THU NGUYEN","email":"","orcid":"","institution":"Academy of Military Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"THI","middleName":"TAM THU","lastName":"NGUYEN","suffix":""},{"id":625604306,"identity":"28ca104e-cc1c-4ea9-b079-24898ee6a54c","order_by":1,"name":"LE THI NHI-CONG","email":"","orcid":"","institution":"Vietnam Academy of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"LE","middleName":"THI","lastName":"NHI-CONG","suffix":""},{"id":625604307,"identity":"6daee22c-5843-46d0-aaef-0739786ab4c5","order_by":2,"name":"THI HUYEN NGA TRAN","email":"","orcid":"","institution":"Vietnam National University, Hanoi","correspondingAuthor":false,"prefix":"","firstName":"THI","middleName":"HUYEN NGA","lastName":"TRAN","suffix":""},{"id":625604308,"identity":"93cc0f4d-47b9-40c8-8610-ae456ff165f9","order_by":3,"name":"KIEN CUONG PHAM","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA50lEQVRIiWNgGAWjYDACHsYGKIv54AMQn48ELWzJBiA+G2EtCJaZBFgnIR3mPIcbHxfUHJYz519gVvk1x06GjYH54aMbeLRY9jY2G884dtjYcsaDtNuy25KBDmMzNs7Bo8XgPGObNG/D4cQNNw4cuy25jRmohYdNmoCW9t8QLQfbiiW31ROh5WxjGzNYy/lmNsaP2w4T1mLZc7BZmudYurHBDTZmacZtx3nYmAn4xZwn/eFnnhprOYPz5z9+/Lmt2p6fvfnhY7wOg7MkEhiYwZHEjEc5qhb+AwyMPwioHgWjYBSMgpEJADNBSJRL/vs/AAAAAElFTkSuQmCC","orcid":"","institution":"Academy of Military Science and Technology","correspondingAuthor":true,"prefix":"","firstName":"KIEN","middleName":"CUONG","lastName":"PHAM","suffix":""},{"id":625604309,"identity":"6a29e211-6150-46c6-bd7e-3b552d3f49a8","order_by":4,"name":"MINH TRI LE","email":"","orcid":"","institution":"Academy of Military Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"MINH","middleName":"TRI","lastName":"LE","suffix":""}],"badges":[],"createdAt":"2026-03-30 09:56:38","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9265218/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9265218/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":109076619,"identity":"8f5d4747-b1f6-40c1-a180-3dfeb337f8a8","added_by":"auto","created_at":"2026-05-12 11:03:51","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":3075645,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptBTNC26.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9265218/v1_covered_693fd924-cfb8-49a7-931f-2f576b66b7e1.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Polyhydroxyalkanoate synthesis ability of the salt-tolerant bacterial strain Salinicola lusitanus BTNC26 isolated from coastal zone in Vietnam and its application in food packaging film production","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Food packaging film, Polyhydroxyalkanoate, Salinicola lusitanus, Salt-tolerant bacterium","lastPublishedDoi":"10.21203/rs.3.rs-9265218/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9265218/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eRecently, polyhydroxyalkanoates (PHA) have garnered systematic attention as biodegradable alternatives to petrochemical plastics. This study aimed to identify bacterial strains synthesizing poly(3-hydroxybutyrate) (PHB) from Vietnam's coastal zones. Strain BTNC26, isolated as a PHA producer, was identified as \u003cem\u003eSalinicola lusitanus\u003c/em\u003e BTNC26 via 16S rRNA gene sequencing (GenBank: PZ087081). Optimization of carbon (glucose) and nitrogen (yeast extract) sources, pH (7), temperature (30\u0026deg;C), and NaCl (5%) enhanced cell growth and PHA accumulation. Under these conditions, biopolymer yield reached 73.07% after 3 days. FTIR, GC\u0026ndash;MS, and NMR confirmed PHB identity: FTIR showed a carbonyl peak at 1710.52 cm⁻\u0026sup1;; \u0026sup1;H/\u0026sup1;\u0026sup3;C NMR revealed resonances at 169.14 (C\u0026thinsp;=\u0026thinsp;O), 67.62 (\u0026ndash;CH\u0026ndash;), 40.80 (\u0026ndash;CH₂\u0026ndash;), and 19.76 ppm (\u0026ndash;CH₃); GC\u0026ndash;MS displayed the 3-hydroxybutyric acid methyl ester molecular ion. Scale-up fermentation (200 mL to 100 L) with 10 g/L glucose supplementation yielded PHB concentrations of 4.76, 4.23, 3.98, and 3.59 g/L at 200 mL, 1 L, 10 L, and 20 L, respectively. Composite films incorporating extracted PHB with PBAT, CaCO₃, PEG, and glycerol exhibited thin, strong, and flexible properties, with biodegradability confirmed in laboratory seawater assays. These findings highlight the potential of halophilic \u003cem\u003eS. lusitanus\u003c/em\u003e for seawater-degradable PHB production, informing strain- and condition-specific strategies for industrial scalability.\u003c/p\u003e","manuscriptTitle":"Polyhydroxyalkanoate synthesis ability of the salt-tolerant bacterial strain Salinicola lusitanus BTNC26 isolated from coastal zone in Vietnam and its application in food packaging film production","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-04-20 18:43:56","doi":"10.21203/rs.3.rs-9265218/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a73f2da3-745a-422a-a2df-1a6cbda2b78f","owner":[],"postedDate":"April 20th, 2026","published":true,"recentEditorialEvents":[{"type":"decision","content":"Rejected","date":"2026-05-12T09:24:45+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-10T18:15:30+00:00","index":29,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-09T12:24:05+00:00","index":28,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-05T06:49:29+00:00","index":27,"fulltext":""}],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-05-12T10:37:48+00:00","versionOfRecord":[],"versionCreatedAt":"2026-04-20 18:43:56","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-9265218","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-9265218","identity":"rs-9265218","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}