Protective mechanism of Scutellarin against pyroptosis network in diabetic retinopathy

Objective: To investigate the regulation and network mechanism of SCU on pyrodeath gene in diabetic retinopathy. Methods The animal model of DM was established and the retinopathy was observed by HE staining, Ni staining and immunofluorescence staining. The retinopathy of SCU group was significantly improved. DR gene, cell pyrophoric gene and astragaloside IV gene were queried, Venny intersection was performed, GO and KEGG analysis was performed, PPI protein interaction was performed, and Hub gene was screened for molecular docking verification. Results 4084 genes related to DR were screened from GeneCards and OMIM databases. Using GeneCards, TCMSP and Swiss Target Prediction databases, 120 SCU therapeutic targets were obtained. Using GenenCards database, 357 targets related to coke decay were retrieved. Drug, disease and phenotypic targets were analyzed online using the Draw Venn Diagram website, and 12 cross targets were obtained. Through GO function and KEGG pathway enrichment analysis of common targets, 659 BP related items, 7 CC related items, 30 MF related items, and 70 signal pathways were screened out. Eleven proteins were screened to interact with the cross-target PPI network, and 11 proteins were subsequently docked with the SCU. The results show that SCU has good binding activity to the core. Conclusion SCU can regulate the death reaction of DR coke through multi-target and multi-pathway, and reveal its possible mechanism.