Culture of HULEC-5a Cells

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Abstract

Abstract This protocol describes the thawing, culturing, and cryopreservation of the human lung microvascular endothelial cell line, HULEC-5a (Mehta et al., 2006). Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use. NOTE: There is a consolidated PDF version of this protocol in the supplementary files section below.

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europepmc
last seen: 2026-05-19T01:45:01.086888+00:00