Research Article MiR-183 Regulates ITGB1P Expression and Promotes Invasion

Copyright © 2015 Jie Chen et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We applied in the previous study miRNA microarray screening analysis to identify several differentially expressed miRNAs, including miR-183 in normal, eutopic, and ectopic endometrium. Knockdown of miR-183 expression induced the invasiveness and inhibition of apoptosis in endometrial stromal cells. The current study aims to identify the miR-183 targets with relevance to cell functions in endometrial stromal cells, to verify the interaction of miR-183 with its target genes, and to confirm the role of miR-183 in the process of endometriosis. Using microarray analysis, we identified 27 differentially expressed genes (19 were upregulated and 8 downregulated), fromwhichwe selected 4 downregulated genes (ITGB1, AMIGO2,VAV3, and PSEN2) based onGOdatabases for functional analysis and significant pathway analysis. Western blotting analyses showed that integrin