Study on Induction of Apoptosis by Fugorgning in Endometriotic Cells

To explore the mechanism of chinese drugs Fugongning on cures of Endometriosis at the molecular level，and to investigate whether apoptosis is induced in endometriosis by the medicine.After making EMT animal model the rats were treated with Fugongning.Danazol and both medicines .Then apoptotic cells were detected by flow cytometry(FCM). 1 .On gross examination the size of transplant in treated groups was significantly decresed as compared with that of control(P<0.05).The growth of Endometriotic cells were inhibited .2 .The apoptosis index in Fugongning, Danazol and the both medicine group was significantly higher than that of control(P<0.)1). 3.After treatment with Fugongning, Danazol and both medicines, there were apoptotic peaks in cell cycle analysis on FCM. The cells in S phase were obvionsly increased(P<0.01),which indicated that cell cycles were hindered in S phase .4. The serum E_(2) level in Fugongning, Danazol and the both medicine group was significantly lower that that of control(P<0.05). This study indicates that Fugongning can induce apoptosis of Endometriotic cells .The possible mechanism is from decreasing the serum E_(2) level to suppress bcl-2 expression in endometriotic cells.