Evaluating the stability of Tritrichomonas foetus in various media under simulated environmental conditions of shipment for RT-qPCR assays.

Boggan S, Williams RB, Koziol JH
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2025 · doi:10.1177/10406387251330297 · PMID:40237409 · PMC12003337
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Abstract

Tritrichomonas foetus is a notable reproductive pathogen in cattle, and the processes of sample collection, handling, transportation, and testing present considerable challenges for surveillance initiatives. Advancements include the development of a direct detection method for T. foetus using reverse-transcription quantitative real-time PCR. We compared the stability of samples in 3 different media: PBS, sterile saline, and lactated Ringer solution over periods of 24-120 h, with storage temperatures of 0°C, 20°C, 37°C, and 54.5°C to mimic shipping of samples in various environmental temperatures.
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