Hepatoprotective effect of Bersama abyssinica Fresen leaf methanolic extract on paracetamol-induced hepatotoxicity in Swiss albino Mice

Hepatoprotective effect of Bersama abyssinica Fresen leaf methanolic extract on paracetamol-induced hepatotoxicity in Swiss albino Mice | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 17 September 2025 V1 Latest version Share on Hepatoprotective effect of Bersama abyssinica Fresen leaf methanolic extract on paracetamol-induced hepatotoxicity in Swiss albino Mice Authors : Shashitu Mekuria , Tesaka Wondimnew , Minale Fekadie , Abebe Dukessa Dubiwak , Nuredin Chura Waritu 0009-0000-1468-4640 [email protected] , Fikadu Seyoum , and Mohammed Jemal Authors Info & Affiliations https://doi.org/10.22541/au.175811054.43793741/v1 Published Global Advances in Integrative Medicine and Health Version of record Peer review timeline 131 views 74 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background: Paracetamol is ones of the popular causes of liver injury mediated by oxidative stress within the liver. Bersama abyssinica is traditionally used to treat liver diseases. However, its effects have not been confirmed by in vivo studies. Objective: This study was aimed to evaluate the effect of a methanolic extract of Bersama abyssinica leaf on paracetamol-induced hepatotoxicity in Swiss albino mice. Methods: The study was conducted on thirty male Swiss albino mice, randomly grouped into; Normal, negative, and positive control groups received 1 ml/kg of distilled water, paracetamol 250 mg/kg, and Silymarin 100 mg/kg orally with paracetamol, respectively. While treatment groups I, II, and III received 200, 400, and 600 mg/kg of the leaf extract with paracetamol 250 mg/kg, respectively. At the end of the experiment, the mice were sacrificed after being anesthetized, and blood samples were collected via cardiac puncture, and the liver was removed for histopathological investigation. Data were analysed using SPSS version 25 and a one-way ANOVA model. Results: The higher levels of ALT, AST, ALP, GGT, and bilirubin were observed in the negative control group than the normal control. However, significant hepatoprotective effects were noted in the treatment and positive control groups, as evidenced by the substantially reduced levels of these liver biomarkers. Abnormal liver architecture was observed in the negative control; however, these changes were reversed following administration of the leaf extract. Conclusion: The higher levels of ALT, AST, ALP, GGT, and bilirubin were observed in the negative control group and reduced after giving the leaf extract of Bersama abyssinicathat makes it to have a hepatoprotective effects against paracetamol-induced liver injury. Supplementary Material File (main manuscript.docx) Download 169.98 KB File (tables.docx) Download 21.81 KB Information & Authors Information Version history V1 Version 1 17 September 2025 Peer review timeline Published Global Advances in Integrative Medicine and Health Version of Record 26 Nov 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Shashitu Mekuria Debre Markos University View all articles by this author Tesaka Wondimnew Jimma University View all articles by this author Minale Fekadie Jimma University View all articles by this author Abebe Dukessa Dubiwak Jimma University View all articles by this author Nuredin Chura Waritu 0009-0000-1468-4640 [email protected] Wolaita Sodo University View all articles by this author Fikadu Seyoum Ambo University College of Medicine and Health Science View all articles by this author Mohammed Jemal Debre Markos University View all articles by this author Metrics & Citations Metrics Article Usage 131 views 74 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Shashitu Mekuria, Tesaka Wondimnew, Minale Fekadie, et al. Hepatoprotective effect of Bersama abyssinica Fresen leaf methanolic extract on paracetamol-induced hepatotoxicity in Swiss albino Mice. Authorea . 17 September 2025. DOI: https://doi.org/10.22541/au.175811054.43793741/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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