Induction of Fresh Chocolate Cyst Pulp into Endometriosis Mice Model

Background & Objective: A chocolate cyst is a type of endometriosis in addition to the appearance of endometrial lesions. The limitations of obtaining these samples led to trying to induce fresh chocolate cyst pulp to become a model of endometriosis mice. Chocolate cyst pulpy can be obtained through cyst puncture with no need to perform laparoscopy or laparotomy. This study was conducted with the aim of proving that induced mice with fresh chocolate cyst pulp samples succeeded in becoming endometriosis models of mice.Materials & Methods: Fresh chocolate cyst slurry was injected as much as 5.10-3 mL/g of mice weight intraperitoneally on day zero. Mice of Mus musculus strain Balb/c were injected with cyclosporine 1.4.10-2 mg/g of the weight of mice intraperitoneally daily for 14 days. On the 1st and 5th day, the mice were injected with estradiol 2.6.10-4 mL/g of the weight of the mice, intramuscularly. Induction research on the endometriosis mice model was conducted at the Pharmacology Laboratory of the Universitas Gadjah Mada, Yogyakarta, Indonesia. On the 15th day, the mice were necropsied for histopathological examination at the Anatomical Pathology Laboratory, Faculty of Medicine, Public Health & Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia, with Hematoxylin-Eosin staining.Results: A total of 6 mice induced with fresh chocolate cyst pulp histopathologically showed signs of endometriosis. Histopathologically readings showed external endometriosis cyst (1 mouse), endometriosis tissue (1 mouse), hemosiderosis (1 mouse), and stromatosis (3 mice).Conclusion: The six mice were induced with fresh chocolate cyst slurry were 100% successful in becoming endometriosis mice models, as a novelty in endometriosis studies using experimental animals