GC-MS Profiling and Antibacterial Activity of Secondary Metabolites from Termite-Associated Bacteria in Ogun State, Nigeria

GC-MS Profiling and Antibacterial Activity of Secondary Metabolites from Termite-Associated Bacteria in Ogun State, Nigeria | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 30 May 2025 V1 Latest version Share on GC-MS Profiling and Antibacterial Activity of Secondary Metabolites from Termite-Associated Bacteria in Ogun State, Nigeria Authors : LAWRENCE OWOLABI 0009-0002-2685-1316 [email protected] , Olusegun Adetoro , Hassan Anthony Olalekan , Rachel Modupe Kolawole , Ani Chiamaka Naomi , and Onita Tolulope Grace Authors Info & Affiliations https://doi.org/10.22541/au.174859219.92281153/v1 178 views 117 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Antimicrobial resistance (AMR) poses a growing global threat, compromising the effective treatment of infections and increasing morbidity and mortality rates. This study investigated the antibacterial potential of bacteria associated with termites as an alternative therapy. Thirty-three termites were processed for bacterial isolation and identification using biochemical tests, BD Phoenix, and 16S rRNA sequencing. Secondary metabolites from isolates were extracted with ethyl acetate and evaluated for antibacterial activity via agar diffusion methods. GC-MS identified bioactive compounds, Insilco technique was performed via STRING analysis. PCR targeting the 16S rRNA gene produced 1.0 kb DNA fragments. Sequencing identified all as Bacillus cereus (99.06–99.77% similarity; Accession Nos. PV660301–PV660305) with homology to GQ406846.1, MT103054.1, and OP830493.1. Antibacterial activity was observed against E. coli (12–15 mm), S. aureus (17–21 mm), Citrobacter sp. (13–15 mm), and S. typhi (12–15 mm), with T2 and T3 showing highest activity against S. aureus (21 mm) and Citrobacter sp . (15 mm), respectively. Ciprofloxacin (control) yielded 16, 24, 17, and 22 mm zones. PV660301–PV660305 produced 17–23 metabolites each, with major compounds including 1-Heptacosanol (53.17%), Squalene (32.45%), Dibutyl phthalate (up to 24.24%), and a Vitamin E derivative (14.77%). STRING network analysis of squalene in S. aureus identified 11 nodes and 55 edges, enriched in steroid biosynthesis (hsa00100), KEGG metabolic pathways (hsa01100, hsa00900), and sterol-related functions, highlighting squalene’s role in membrane integrity and virulence. Termite-associated Bacillus cereus showed strong antibacterial potential, warranting further elucidation of the metabolite via NMR Supplementary Material File (termite manuscript willey implemented.docx) Download 628.41 KB Information & Authors Information Version history V1 Version 1 30 May 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords bacillus cereus and termite-associated antibacterial activity gc-ms secondary metabolites Authors Affiliations LAWRENCE OWOLABI 0009-0002-2685-1316 [email protected] Babcock University Benjamin S Carson Snr School of Medicine View all articles by this author Olusegun Adetoro Moshood Abiola Polytechnic View all articles by this author Hassan Anthony Olalekan Moshood Abiola Polytechnic View all articles by this author Rachel Modupe Kolawole Lagos State University Department of History and International Studies View all articles by this author Ani Chiamaka Naomi Moshood Abiola Polytechnic View all articles by this author Onita Tolulope Grace Moshood Abiola Polytechnic View all articles by this author Metrics & Citations Metrics Article Usage 178 views 117 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation LAWRENCE OWOLABI, Olusegun Adetoro, Hassan Anthony Olalekan, et al. GC-MS Profiling and Antibacterial Activity of Secondary Metabolites from Termite-Associated Bacteria in Ogun State, Nigeria. Authorea . 30 May 2025. DOI: https://doi.org/10.22541/au.174859219.92281153/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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