Scanning Various Nanomaterials and Variable Camouflages to Evaluate the Biocompatibility in Multi-organs of Embryos

Abstract Background:Nanomaterials are under a wide range of application prospects in human health and disease, such as medical imaging and drug delivery and treatment. With the gradual advent of the nano era, the safety of nanomaterials in biology must be evaluated more widely and deeply. Therefore, superficial safety assessment based on specific cell lines in vitro is difficult to meet the current needs. Multi-organs assessment in vivo will be beneficial in establishing a more comprehensive pathway for the understanding of nanomaterials-induced biotoxicity. Results: In this work, we employed a series of genetically modified zebrafish models for nanomaterials toxicity assessment. The results demonstrated that cadmium selenide (CdSe) quantum dot (QDs) was the most toxic after scanning some popular nanomaterials. Among modified methods of silica coating, core-shell structure development and organic molecular camouflage, the polyethylene glycol camouflage method exhibits a better performance of improving the biocompatibility of CdSe QDs in multiple-organs including hearts, nerves, blood vessels, and immune system. Conclusion: Our work provides an alternative paradigm for more in-depth and sensitive preclinical validation of biocompatibility, especially in neurotoxicity and cardiotoxicity of embryos, and a guidance for reducing the toxicity of biomedical materials.