First record for an alien oriental river prawn Macrobrachium nipponense(De Haan, 1849) (Decapoda: Palaemonidae) distribution in the lower Danube, Bulgarian part confirmed with DNA barcoding

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Abstract

The alien shrimp Macrobrachium nipponense (De Haan, 1849), known as the oriental river prawn, has been identified in the Bulgarian part of the Lower Danube for the first time, based on morphological characteristics and DNA barcoding. Four specimens were caught in October 2023 in the Danube River near Pozharevo island (422.2 rkm). The diagnosis of the species was based on the number of teeth on the dorsal and ventral side of the rostrum, the large size of the second pair of pereopods, and the presence of the hepatic spine. A fragment of the cytochrome c oxidase subunit I (COI) gene of mitochondrial DNA was sequenced to supplement species identification. Phylogenetic analysis has revealed a high genetic similarity (over 99%) between the Bulgarian sample and samples from various geographic locations (Ukraine, China, Bangladesh, Iran, Iraq, and Russia). The record of the oriental river prawn confirms its wide expansion and successful naturalization in the Lower Danube region.
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Preprint ARPHA Preprints https://doi.org/10.3897/arphapreprints.e143823 (11 Dec 2024) https://doi.org/10.3897/arphapreprints.e143823 (11 Dec 2024) Published in: Nature Conservation https://doi.org/10.3897/natureconservation.58.142047 Other versions: - Preprint InfoPreprint Info - CiteCite - MetricsMetrics - CommentComment - RelatedRelated - CitedCited ARPHA Preprints doi: 10.3897/arphapreprints.e143823 First posted 11 Dec 2024 Author Violin Raykov - Corresponding author Institute of Oceanology - Bulgarian Academy of Sciences, Varna, Bulgaria Conflict of interest The author has declared that no competing interests exist. This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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