ULI-NChIP assay protocol

Abstract This protocol presents ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) assay to generate high quality and complexity genome-wide histone mark profiles from rare oocytes andembryos populations. The procedure of ULI-NChIP-seq assay typically consists of five parts including Binding antibodies to magnatic beads, Chromatin shearing and nuclear membrane solubilization, Magnetic immunoprecipitation, Washes and DNA isolation. Sample preparation involves to remove the zona Pellucida of oocyte and polar body to avoid the genomic contamination of polar bodies.