Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia

Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia Yuan Fang, Tambo Ernest, Jing-Bo Xue, Yi Zhang, Xiao-Nong Zhou, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-35782/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract Background: Gene mutations on target sites can be a valuable reference to the status of insecticide resistance. Jeddah, a global commercial and major port-of-entry city, is bearing the most (>70%) dengue disease burden and the population of the dengue vector Aedes aegypti (Linnaeus, 1762) in Saudi Arabia. Culex quinquefasciatus Say, 1823 is a second example as one of the major arboviral vectors in the region. However, the status of insecticide resistance and targeted site mutations on the responsible genes are not fully characterized. Methods: We randomly sampled both mosquito species, Ae. aegypti (25 individuals) and Cx. quinquefasciatus (24 individuals) across Jeddah by daily mosquito surveillance in 2016 to detect the resistance-associated target site mutations on the voltage gated sodium channel ( VGSC ) and acetylcholinesterase 1 ( ace-1 ) genes by PCR amplification and direct sequencing. Results: Our findings showed that Ae. aegypti resistance-associated VGSC gene mutations revealed polymorphic mutations on the 989, 1016, and 1534 sites. Additionally, we documented two types of introns between exons 20 and 21, however, the I1011M point mutation was undetected. Linkage disequilibrium associations were shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. Furthermore, no mutation on ace-1 was identified in Ae. aegypti . In Cx. quinquefasciatus , homozygous L1014F/L1014F (95.23%) on the VGSC and heterozygous G119/G119S (100%) on ace-1 were widely distributed in the samples studied. Analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (P = 0.000) allele type, and may reflect the history of insecticide treatment in different continents. Conclusions: To the best of our knowledge, this is the first record of the intron types between exons 20 and 21 on VGSC of Ae. aegypti populations. It can serve as a reference marker to evaluate the local insecticide resistance status. In addition, the first report of insecticide resistance gene mutation being present in field caught Cx. quinquefasciatus in Saudi Arabia. High prevalence of insecticide resistance gene mutations in local primary mosquito vector species alert the urgent need to carry out a comprehensive insecticide resistance surveillance. Parasitology acetylcholinesterase 1 gene dengue knockdown resistance gene voltage gated sodium channel gene Figures Figure 1 Figure 2 Introduction Mosquitoes-borne diseases, including dengue, malaria, West Nile, Japanese encephalitis, Rift Valley fever, Chikungunya, Zika, and other emerging arboviral diseases are prevalent and are enormous threat to public health worldwide. Intensive international trade and travel, influenced by global economic integration, accelerated the spread of mosquito-borne viruses, resulting in occasional disease outbreaks even in previously endemic or disease-free areas. Since no protective vaccines and effective drugs are available for majority of these infectious diseases, the strategies on reducing the risk of transmission is mainly focused on mosquito vector control. Vector control has been relying heavily on insecticides application to reduce mosquito larval and adult populations in their natural habitats as well as personal protection using anti-mosquito repellents [ 1 ]. Selection pressure on insecticide target sites of mosquito vectors, resulting in the occurrence and developing of single or multiple-site mutations in the responsible genes have been reported and subsequently detected globally in major mosquito vector species belonging to the genera Anopheles , Aedes and Culex in the last few decades [ 2 , 3 ]. Increased insecticide target site insensitivity due to gene point mutations, and the increased or elevated activity of insecticide detoxifying enzymes are two of the major mechanisms evolved and developed by mosquito vectors for surviving the lethal effect of insecticides [ 4 , 5 ]. At least four vector-borne viruses, including dengue virus (DENV), Alkhurma hemorrhagic fever virus, Rift Valley fever virus, and Crimean-Congo hemorrhagic fever virus have been circulating in Saudi Arabia [ 6 ]. The most important of them is dengue, with most of the disease burden and severity, mainly prevalent in the western and southwestern regions in Jeddah and Makkah, of Saudi Arabia [ 7-9 ]. A total of 5,345 confirmed dengue cases were reported in 2018, with incidence rate of 16/100,000 population, with most of cases (92.46%, n = 4,942) in Jeddah and 3.05% (n = 163) in Makkah, according to the Ministry of Health of Saudi Arabia recent reports [ 10 ]. Jeddah is the financial and diplomatic center in Saudi Arabia, and is the major point-of-entry to the Holy city of Makkah (only 80 km SE of Jeddah) for millions of visitors of perennial religious rituals for pilgrimage (Hajj) and Umrah as well as workers and other visitors. Aedes aegypti (Linnaeus, 1762), the major mosquito vector of dengue, chikungunya and Zika, and Cx. quinquefasciatus Say, 1823, vector of West Nile, Rift Valley fever and Japanese encephalitis, are two dominant mosquitoes in Jeddah [ 11 , 12 ]. Although there have been no reports of West Nile virus outbreaks in Jeddah, it has been reported in countries neighbouring Saudi Arabia, such as Pakistan, Iran, Egypt and Israel [ 13-15 ]. Since the distribution of the two vectors Ae. aegypti and Cx. quinquefasciatus are widespread and both are domestic and endophagic species, there is an ever-present risk of arboviral disease outbreaks. In our previous paper, DENV-2 virus and four strains of insect-specific flaviviruses, including one of cell-fusing agent virus (CFAV) and three of Phlebotomus-associated flavivirus (PAFV) were detected in pools of Ae. aegypti , and 10 strains of Culex flavivirus (CxFV) in pools of Cx. quinquefasciatus population from Jeddah[ 16 ]. Meanwhile, the two species suffered from extensive and long-time insecticide exposure for routine mosquito control operations using larvicides spraying of their natural breeding habitats and indoor residual spraying (IRS) using adulticides, resulting in developing of insecticide resistance, which subsequently reduced the efficacy of insecticide treatments [ 7 , 9 , 15 , 17 ]. The extensive use of insecticide result in the occurrence and development of insecticide resistance in local populations. Investigations on the pattern of pesticide uses in integrated vector management (IVM) programs revealed that chemical insecticides control has been the last resort and major control approach of mosquito vector and public health pest in the Kingdom. Insecticide application methods were mainly space spraying (e.g., fogging, ULV), larviciding residual spraying. Over a period of 20 years, an overall volume of insecticides used in IVM were 64,000 liters, 140,000 liters and 268,868 liters (mainly pyrethroid and organophosphate insecticides) from 2002–2006, 2007–2012 and 2012 to date by the government and others private contractors in routine mosquito control operations, respectively. The pyrethroid group (mainly deltamethrin, cypermethrin, and cyfluthrin), sporadically the organophosphates pirimiphos-methyl and diazinon, have been the most common insecticides used in different commercial formulations over the last 20 years in the Kingdom. Aedes aegypti populations from Jeddah and Makkah showed high resistance to permethrin, deltamethrin, bendiocarb, and were tolerant to fenitrothion, based on conventional insecticide bioassays performed on adult mosquito samples collected in 2015 [ 18 ]. Noteworthy, in a tenf-year period, the mortalities to pyrethroids decreased from 75–93% in 2008 to < 20% in 2017 in the Ae. aegypti population in Makkah [ 18 ]. In addition, the Jazan population was only sensitive to cyfluthrin, tolerant to deltamethrin, permethrin, fenitrothion, and resistant to Lambda-cyhalothrin, DDT, and bendiocarb [ 19 ]. Whereas, resistance to DDT, permethrin and deltamethrin in Cx. quinquefasciatus from Jeddah developed as early as 1984 [ 20 ]. However, to our knowledge, no relevant record reported on insecticide resistance in Cx. quinquefasciatus from Saudi Arabia after then, especially in field-caught individuals. The most prevalent insecticide resistance gene mutation is the knockdown resistance ( kdr ) mutation on the para voltage sodium channel gene ( VGSC ). The mutation sites on the amino acid 1014 position on VGSC IIS for both Anopheles and Culex , and 1016 position for Aedes , were well confirmed to reduce the mosquito sensitivity to pyrethroid, and the organochlorine DDT due to cross-resistance effect [ 21-23 ]. In specific, several point mutation sites on VGSC are speculated to get involved in pyrethroid and DDT resistance in Ae. aegypti , including G923V, L982W, S989P, M1011I/G, V1016G, F1269C in domain II, and T1520I, F1552C, F1534C, D1763Y in domain III of VGSC [ 1 , 24-26 ]. Among them, two point mutations, V1016G and F1534C that confer resistance to pyrethroids and DDT, are the most widespread [ 27-32 ]. Four non-synonymous (A99S, L1014F/S/C, V1016G, W1594R) and six synonymous, (L884L, G923G, A1262A, D1266D, P1270P, G1754G) mutations were found to be linked with the levels of permethrin resistance in Cx. quinquefasciatus populations [ 33-35 ]. In addition, the replacement of the amino glycine (Gly, G) with serine (Ser, S) on position 119 of acetylcholinesterase 1 ( ace-1 ) was found to be associated with mosquito resistance to organophosphate and carbamate insecticide groups [ 36 ]. The G119S mutation was commonly detected in Anopheles and Culex , while seldomly recorded in Aedes species [ 1 ]. In Saudi Arabia, S989P, V1016G and F1534C mutations on VGSC were reported in Ae. aegypti samples collected from Jeddah and Makkah in 2015 [ 18 ]. It is the first on insecticide resistance gene detection in Ae. aegypti populations from Saudi Arabia. However, little is documented on the trend and impact of insecticides application in IVM programs implementation nearly for 20 years (1995–2016) on vector-borne disease threats and epidemics prevention and control in the Kingdom of Saudi Arabia. Additionally, point mutations in the VGSC gene of Ae. aegypti populations in Jeddah has not been fully characterized, especially the types of introns between exons 20 and 21, as well as whether the ace-1 mutations in Jeddah Ae. aegypti populations. In Cx. quinquefasciatus , the V1016G mutation was detected in the JPP-B strain, originated from Jeddah and selected by permethrin for 20 consecutive generations [ 20 , 34 ]. This mutation has not been reported in other populations of Cx. pipiens complex [ 37 ]. To the best of our knowledge since the first studies performed in the mid of 1980s, no study on insecticide resistance gene(s) in Cx. quinquefasciatus populations have been reported in the so far in Saudi Arabia. Thus, currently, there are limited information on insecticide resistance gene mutations in Cx. quinquefasciatus against chemical insecticides in Jeddah. For the bioassay test requires numerous live mosquitoes and time-consuming [ 38 ]. Early detection and characterization of target mutations of insecticide resistance genes, as potentially valuable molecular markers, can provide a reference to estimate the local insecticide resistance level for monitoring, planning and evaluation of vector control strategy [ 1 , 39 , 40 ]. This study aimed to determine the status of insecticide resistance in Ae. aegypti and Cx. quinquefasciatus mosquito vectors, by mapping the point mutations associated with kdr mutations and the phylogeny of the introns between the exons 20 and 21 on VGSC , and ace-1 . The results of our study will be very important for the early-detection of resistance development and in guiding IVM and insecticide application policies for the prevention and control of mosquito-borne diseases in Saudi Arabia. Materials And Methods Mosquito Collection A total of 25 Ae. aegypti and 24 Cx. quinquefasciatus female mosquitoes were analyzed, representing different districts and municipalities of Jeddah Governorate. These mosquitoes were collected during routine mosquito surveillance in 2016. Collection sites for detection of target site mutations on insecticide resistance genes are shown in Fig. 1, and a map was generated using ArcGIS 10.1 ArcMap software (ESRI, Redlands, CA, USA). The species name, geographic information (including the latitude and longitude information), and GenBank accession numbers of each randomly selected samples are shown in Additional file 1. DNA extraction and target gene mutation sequencing For DNA extraction, individual mosquito samples, each was homogenized in a mixer mill (Jingxin, Shanghai, China) in 250 μl of ATL (Qiagen, Hilden, Germany) and 20 μl of proteinase K (Qiagen, Hilden, Germany) with two 3-mm steel balls were added to each tube. The homogenates were incubated at 56 °C overnight in an oscillating thermo-block. The homogenate tubes were then centrifuged at 1000 × rpm for 3 min at room temperature. A 200 μl aliquot of supernatant from each homogenate was added to the Roche ® MagNA Pure 96 sample plate, and placed into to the MagNA Pure 96 System for automated DNA extraction (MagNA Pure 96 DNA and Viral NA Small Volume Kit, Roche, Basel, Switzerland) according to the manufacturer’s instructions DNA was eluted in 100 μl of buffer solution. For Ae. aegypti , the primer set IIS5-6(3)F and IIS5-6(3)R were used to amplify the IIS6 domain of the VGSC , spanning the codons 989, 1011, 1016, and the introns between exons 20 and 21; while the codon 1534 domain IIIS6 was amplified by the primer set AaNa31F and AaNa31R [ 41 ]. The ace-1 gene region spanning the position 119 was amplified by the primer set Ace-1F and Ace-1R [ 42 ]. For Cx. quinquefasciatus , the VGSC region spanning the 1014 codon was amplified by the primer set Cq1 and Cq2 [ 43 ]. The fragment containing the ace-1 mutation was amplified by the primer set Moustdir and Moustrev [ 44 ]. The PCR amplification products were separated on 1.5% agarose gel electrophoresis, purified, and sequenced in both directions by Sangon Biotech (Shanghai, China). The peak spectrum (chromatogram) of the sequencing data was determined using Bio-edit v.7.2.6 [ 45 ]. The allelic information for certain ambiguous sequences, cloning and plasmid sequencing were performed for confirmation. Phylogenetic analysis The obtained VGSC IIS6 fragments spanning the introns flanked by exons 20 and 21 were aligned with available homologous sequences retrieved from GenBank using ClustalW2 [ 46 ] with default settings, which were manually adjusted when was necessary. Based on the Akaike information criterion, the best-fit model for the alignment was determined using Model test 3.7 [ 47 ], in cooperation with PAUP* v.4.0b10 [ 48 ]. Consequently, calculation of the maximum likelihood (ML) and Bayesian likelihood trees were completed under the TVM+ G model for introns between exons 20 and 21 on the VGSC . Both the ML and neighbour-joining trees were constructed using MEGA v.7.0 software [ 49 ], with 1,000 bootstrap replicates. The Bayesian tree was built with MrBayes v3.2.6 [ 50 ] on the CIPRES portal ( www.phylo.org/ ) [ 51 ], run for 10 million generations, with the first 25% generations discarded as burn-in. The trees were visualised using Figtree v1.4.2 ( http://tree.bio.ed.ac.uk/software/figtree/ ). Both intra- and inter-specific divergences of introns spanning between exons 20 and 21 were calculated using K2P distance model [ 52 ] in MEGA v.7.0 . Statistical analysis Pearson’s Chi-squared test was applied to analyze associations among mutation codons and type of introns between exons 20 and 21 on VGSC . The tests were performed in SPSS v.20 (IBM Corp., Armonk, NY, USA). Results Mapping of the target resistance-associated genes in Ae. aegypti and Cx. quinquefasciatus The target resistance-associated genes successfully amplified from a total of 20 Ae. aegypti and 21 Cx. quinquefasciatus . The sequences originally obtained in this study have been deposited in the GenBank under the accession numbers MN997310–MN997399. Three non-synonymous VGSC mutation alleles, S989P, V1016G and F1534C were identified from specimens of wild Ae. aegypti . The presence of a silent mutation (TTG to TTA) at position (Leu, L) 982 (14/20) was found. No I1011M and F1552C mutations were identified. In addition, two types of introns between exons 20 and 21, type A (250 bp) and type B (234 bp) were identified. Six genotypes of the combination 989, 1016, and 1534 positions were documented including SVF/PGF, SVF/SVC, SVC/SVC, PGF/PGF, PGF/PGC, PGC/PGC (Table 1). Among them, PGF/PGC was the dominant genotype, detected in 45% of the examined samples, followed by the SVC/SVC genotype with 25%. Genotypes of SVF/PGF, SVF/SVC, and PGC/PGC were observed less frequently. Allele frequencies are shown in Table 2. The V1016G mutation seems to be more widely distributed with high frequency (67.50%) in Jeddah, than that of the F1534C mutation (55.00%). Both TTG982TTA (χ 2 = 22.000, df = 2, P < 0.001), and S989P (χ 2 = 44.000, df = 4, P < 0.001) co-occur with the presence of V1016G. All TTG982TTA, and S989P coexist with the haplotype harboring the V1016G mutation. In individuals carrying homozygous F1534C mutation in the IIIS6 region, no coexistence of other mutations on 982, 989, and 1016 codons in IIS6 domain was observed. The type A intron between exons 20 and 21 was strongly linked to the V1016G mutation. The haplotype with type A intron was in association with V1016G mutation (χ 2 = 22.00, df = 2, P < 0.001) and wild-type F1534 (χ 2 = 11.75, df = 2, P = 0.003). By contrast, the F1534C mutation was strongly linked with the type B intron with all of the SVC/SVC genotype linked with the type B intron. The allele type on 1016 position was highly correlated with the other two mutations, 989 and 1534, especially the 989 position (χ 2 = 44.00, df = 4, P < 0.001). All homozygous V1016G mutations linked with homozygous S989P mutation, V1016G heterozygous mutation linked with heterozygous S989P mutation, whereas wild-type of V1016 is linked with the S989. Individuals possessing homozygous wild-type V1016 harbored homozygous (5/6) or heterozygous (1/6) of the F1534C mutation, however, no wild-type homozygous F1534 coexisted. Phylogenetic analysis of intron types identified in the voltage-gated sodium channel gene ( VSGC ) of Aedes aegypti The intron located between exons 20 and 21 on VGSC identified in our study and those retrieved from the GenBank were compared and used to calculate the evolution of the kdr mutations. The studied 158 sequences can be clearly divided into two clades with high bootstrap values, one clade contained the sequences possessing type A intron, while the second clade contained those sequences having type B intron (Fig. 2). The intra-group genetic distances of type A and type B introns were 0.001 and 0.049, respectively, whereas the inter-group was 0.355. The two intron types are detected in four continents, Africa, Asia and Latin and North America, suggesting that they were distributed paraphyletically. In Latin America (Brazil, El-Salvador, Guatemala) and North America (USA: California and Hawaii), the ratio of type A to type B intron were 5:1 and 4:0, respectively; while in Asia (China, India, Indonesia, Malaysia, Myanmar, Philippines, Saudi Arabia, Singapore, Sri Lanka, Thailand, Viet Nam), the ratio was 88:37; and in Africa (Ghana, Kenya, Malawi, Nigeria, Zambia, Zimbabwe), the ratio was 4:17. In addition, for global analysis the associations among mutation codons and type of introns between exons 20 and 21 on VGSC , we added specific 989, 1016 and 1534 allele types of those sequences, available from other studies. The results of analysis of the associations between the three codons and the intron types reported, showed that the allele type of 1016 position was more related to the 989 position (χ 2 = 49.910, df = 4, P < 0.001) than to the 1534 position (χ 2 = 16.297, df = 4, P = 0.003). While the intron polymorphism was more correlated to the 1016 position (χ 2 = 34.333, df = 2, P < 0.001) than the 1534 position (χ 2 = 18.005, df = 2, P < 0.001) (Table 3). Point mutations and polymorphisms on VGSC and ace-1 in Cx. quinquefasciatus The majority of the entire successfully sequenced partial VGSC gene with kdr mutation in Cx. quinquefasciatus was the homozygous genotype TTT/TTT (90.48%), except two of them was the heterozygous genotype TTT/TTA. All the detected ace-1 gene was the heterozygous genotype GGC/AGC. Discussion The application of IRS and larvicides to reduce and control populations abundance of Aedes , Culex and Anopheles mosquitoes responsible for dengue, Rift Valley, West Nile and malaria infections witnessed significant decline in the years 1970s and 1980s. With the persistent mosquito-borne disease foci and endemicity in the major Saudi cities, Jeddah, Makkah, Madinah, Aseer and Jazan, in the South-Western and southern borders regions, renewed governments efforts ushered in enhanced pest surveillance coupled with integrated vector control programs implementation showed significant decline of total national cases from 2000 till now. However, as a side-effect, insecticide resistance in local populations has occurred and developed. In the current study, three non-synonymous point mutations on 989, 1016 and 1534 positions and type A and B introns on VGSC were reported detected in field-caught Ae. aegypti mosquito samples in 2016 from Jeddah, Saudi Arabia. No samples that harbored the wild-type at the three mutation loci simultaneously were identified. The I1011M/V mutation was absent in Jeddah, even though this substitution of isoleucine on 1011 position is prevalent in Latin America countries [ 39 , 41 , 53-55 ], and spread in Thailand and Vietnam but at low frequencies [ 56 , 57 ]. So far, to our knowledge, Saudi Arabia is the place with the most variation in kdr mutations associated with high resistance to pyrethroids in the Middle East countries, probably due to long-term usage of high doses of pyrethroids in vector control programs in and all major cities, especially in Jeddah since the first dengue epidemics in 1994. This is different from the reported kdr polymorphisms of Ae. aegypti populations from India and Pakistan. High prevalence of the F1534C mutation, and a low frequency of I1011M/V and V1016I mutations, with the absence of the 989P allele, were observed in India [ 25 ]. In Pakistan, all the studied mosquito samples harbored the homozygous F1534C mutation, whereas no mutation on the 1016 position has been identified yet [ 58 ]. The percentage of homozygous V1016G and S989P mutations co-occurrence was high (65.0%) in Jeddah. The V1016G mutation plays a critical role in kdr resistance against pyrethroid compounds [ 22 , 23 , 58 ]. Linkage association has been observed between the 989 and 1016 loci in Ae. aegypti populations of Saudi Arabia [ 18 ], Myanmar [ 59 ], Thailand [ 60 ]. It is speculated the S989P may serve as a compensatory mutation to the V1016G, to reduce the fitness cost on the mosquito [ 22 ] and strengthen the response of V1016G to permethrin and deltamethrin [ 61 ]. Additionally, double homozygous mutations at positions 989 and 1016 combined with either wild-type or heterozygous mutation at 1534 position were common in Jeddah’s population, in agreement with the speculation that the 1016 and 1534 loci were not independent for linkage disequilibrium analysis [ 62 ]. Further, the genotype with the triple mutation haplotype predominated in the tested mosquito samples, with an occupation of 45% (PGC/PGF). Additionally, one individual possessing triple homozygous mutation on 989, 1016, and 1534 loci was found in this study. In field-collected mosquitoes, the triple homozygous mutation was in low frequency in the population [ 32 , 58 , 59 , 63 , 64 ], probably due to fitness cost exerted on the mosquito by this triple mutation [ 61 , 65 ]. Whereas, the artificially-introduced triple mutation, using the Xenopus oocyte expression systems, appeared to confer a higher level of resistance to both permethrin and deltamethrin than those carrying the individual mutations, probably for synergistic effect of the combination of mutant alleles [ 22 , 23 , 61 ]. Li et al., [ 30 ] suggested that the F1534C mutation could possibly serve as a compensatory mutation for reducing the fitness cost on the mosquito induced by the V1016G mutation. Thus, whether the homozygous triple mutations on 989, 1016, and 1534 loci showing insecticide resistance in field mosquitoes is yet to be ascertained. The intron polymorphism of VGSC could serve as a marker to study the evolution of kdr mutations [ 38 , 53 ]. Based on sequence size, the introns located between exons 20 and 21 were classified into type A (250 pb) and type B (234 pb) [ 53 ]. Analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (χ 2 = 34.333, df = 2, P = 0.000), and 1534 (χ 2 = 18.005, df = 2, P = 0.000) allele type. Further, all the individuals possessing the V1016G mutation, either heterozygous or homozygous harbored the type A intron, but not vice versa. This result is in contrast to Saavedra-Rodriguez et al.,’s [ 39 ] suggestion that the V1016G mutation was distributed independently in haplotypes possessing either the type A or type B intron. Additionally, the global data analysis showed that the two intron types were distributed evenly in both heterozygous or homozygous F1534C mutation, but the homozygous wild-type F1534 was mainly associated with the type A intron, and not vice versa as well. In Jeddah, six of the examined mosquito individuals were harboring the homozygous F1534C mutation. Among them, five individuals with the SVC/SVC genotype possessed the type B intron, while the individual carrying the homozygous triple mutation (PGC/PGC) was coupled with the type A intron. In Taiwan Province, China, the F1534C was found to be strictly associated with the type B intron, and the V1016G mutation coexisted with the type A intron [ 38 ]. By contrast, in Africa, the point mutation at 1534 site was found to be strongly linked with the type A intron in Ghana, but only one heterozygote point mutation V1016I was recorded, except the F1534C mutations [ 66 ]. Additionally, V1016I was reported to locate at the haplotype possessing the type A intron, with no 1534 variation observed in Brazil [ 53 , 67 ]. The mutation at position 1016 is associated with resistance to both type I and II pyrethroids, while the F1534C allele is primarily associated with resistance to type I pyrethroids, and the V1016I mutation did not alter channel sensitivity to pyrethroids in Xenopus oocytes [ 22 , 23 ]. Based on the evidences above, we speculate that kdr mutations incline to coexist with the type A intron. When a mutation occurred in either 1016 or 1534 positions, it would be linked to the type A intron. While in case of co-mutation, the type A intron distributes more frequently with the V1016G/I mutation rather than the mutation F1534C. It is probably due to balancing the fitness cost on the mosquito by increasing compensatory advantage or linkage disequilibrium [ 61 , 68 ]. It is speculated that the type B intron is the ancestral clade of clade 1, because that the Ae. aegypti was originally from Africa and the majority of African individuals belong to type B [ 66 , 69 ]. In view of the phylogenetic tree obtained with available global data of the intron sequences, 100%, 83.33% and 70.40% individuals from North America, Latin America and Asian countries respectively, were type A intron. By contrast, 73.91% of sequences from Africa belonged to the type B intron. According to our analyses above, the type A intron coexistence with kdr mutations is common, especially the V1016G mutation; while the type B couples with the wild-type or F1534C mutation when the V1016G mutation is absent. This variable pattern of association, may reflect the history of insecticide treatment in different continents. The major disease burden of malaria is in Africa and South-East Asia [ 70 ]. The mutation on 1534 locus may have arisen in Aedes species due to earlier or current use of DDT for larvicides spraying and IRS to control malaria since the middle of the last century [ 41 , 71 ]. Whereas both in Latin and North America countries, vector control is mainly focused on Aedes species (mainly Ae. aegypti and Ae. albopictus ) vectors of dengue, Chikungunya, yellow fever, and Zika [ 72 ]. For the domestic and endophagic habitats of Ae. aegypti , pyrethroid insecticides are widely and randomly used in household aerosol insecticides for public and personal protection, since that pyrethroids have the advantages of high efficacy against mosquito vectors, low mammalian toxicity and short residual action [ 73 ]. In South Asia countries, the higher frequency of type A intron than that of type B may indicate different selective pressures resulting in different histories of intense and unrestricted insecticide usage. It is probably that first, the F1534C mutations occurred due to the extensive use of DDT or type I pyrethroids, as both insecticides have the same target site [ 41 ], then V1016G has been selected by the subsequent and wide use of type II pyrethroids, as cypermethrin and deltamethrin [ 59 ]. Both the non-synonymous mutation G119S (GGC/AGC) and silent mutation T506T on ace-1 [ 42 ] have not been detected in our sequences in Ae. aegypti samples from Jeddah. While it does not indicate that the Ae. aegypti population was completely susceptible to organophosphate and carbamate compounds, as both types of insecticides have the same target site. In bioassay tests, the Ae. aegypti population from Jeddah was tolerant to the fenitrothion [ 18 ], indicating that other than kdr mutations, or more likely, enzymatic mechanisms are involved in conferring resistance in mosquitoes. In Central Java, Indonesia, although the G119S mutation was absent, a degree of resistance to malathion was observed in Ae. aegypti [ 1 ]. Reports on target-site mutations on VGSC and ace-1 genes of Cx. quinquefasciatus are rare compared of that on Ae. aegypti . However, the insecticide resistance level of the Cx. quinquefasciatus in Jeddah may even worse as inferred from the fixation of mutation allele both on VGSC and ace-1 genes. In the studied mosquito samples in Jeddah, the kdr homozygous genotype TTT/TTT occupied most (90.48%) of the individuals. While the heterozygous genotype GGC/AGC on ace-1 gene was detected in all samples of Cx. quinquefasciatus . This is probably related to constant exposure due the extensive use of insecticides for routine spraying campaigns of public health, or for domestic use against mosquito vectors and other insect pests in Jeddah region. Similarity, the homozygous susceptible 1014 genotype was absent in samples collected in 2011 from Zanzibar, East Africa [ 74 ]. In India, most of the studied mosquito samples harbored the heterozygous genotype TTT/TTA, with the frequency of 75% in Cx. quinquefasciatus samples [ 43 ]. Limitations of this study One major limitation of our study is the small sample sizes of mosquitoes used for detection on target site mutations of insecticide resistance genes. Whereas, the primary survey reveals the widespread of kdr in predominant mosquitoes in Jeddah. Further, bioassay tests on both larvae and adult mosquitoes are required to evaluate the status of insecticide resistance in practice. And the underlying metabolic mechanism responsible for insecticide resistance is worth exploring. Conclusions The current study involved a wider geographic region of Ae. aegypti vector populations in Jeddah. The results showed that six genotypes of the three codon combinations, 989, 1016, and 1534 were spreading in Jeddah’s Ae. aegypti population, with the PGF/PGC as the dominate one. Two types of introns between exon 20 and 21 on VGSC for the first time are identified in Saudi Arabia. Linkage associations have been shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. High prevalence of kdr mutations implies that the local Ae. aegypti mosquitoes are pyrethroid-resistant, and the level of resistance may increase with the extensive and intensive use of pyrethroid, as the active ingredients in household insecticide and spatial repellents. To the best of our knowledge, this is the first report of resistant-associated mutations in field caught Cx. quinquefasciatus in Saudi Arabia. The fixation of L1014F allele on VGSC and G119S on ace-1 gene was detected in local Cx. quinquefasciatus populations, with the frequency of 95.24% and 100%, demonstrating that traditional insecticides are likely to be losing efficacy through fogging activities or IRS. In addition, analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (P = 0.000) allele type, and may reflect the history of insecticide treatment in different continents. The primary analyses showed the widespread of kdr mutation both in Ae. aegypti and Cx. quinquefasciatus . The situation may even worse with increasing urbanization, climate changes and sustained Aedes-Culex endemicity estimated at 31–56% prevalence rate, thus threating the efficiency of vector control against mosquito-borne diseases. It raises the attention that wide-range of surveillance the polymorphism and distribution of insecticide target site mutations in mosquitoes in this region. The mapping of the nation-wide distribution and polymorphism of target site mutation against insecticide resistance will provide valuable consensus for forecasting the status of insecticide resistance in mosquito populations of Jeddah and other regions of Saudi Arabia. Additionally, regular monitoring and documenting the dynamics of target mutation gene and their associations with the insecticide resistance phenotype is important in the surveillance system of preventing mosquito-borne diseases through long-term and sustainable vector control strategies. Meanwhile, the above findings suggest that alternative insecticides in areas, and proper use of insect growth regulator or biocontrol approaches for integrated vector control programs should be considered in Jeddah and neighborhoods to mitigate and slow down the spread and impact of insecticide resistance development in disease vector populations. Albeit, substantial Saudi government and Jeddah municipality commit and invest in innovative operational research, research and development on more early-detection of insecticide resistance and effective control measures and tools for discovery and utilization, including vaccines for local and pilgrims’ immunization programs implementation. With the establishment of the Public Health Pest Laboratory of Jeddah Governorate, sustainable funding is imperative to provide evidence for coherent and coordinated temporal and spatial epidemiological IVM and pesticide choices implementation amongst all stakeholders. Furthermore, strengthening laboratory and epidemiologic capacity building programs for health workers, health professional, and community engagement including and private-public sectors partnerships on mosquito vector, dengue and emerging threat and epidemics sustained control towards elimination. Abbreviations ace-1 : acetylcholinesterase 1; DENV: dengue virus; kdr : knockdown resistance; K2P: Kimura’s 2-parameter; ML: maximum likelihood; IRS: indoor residual spraying; IVM: integrated vector management; VGSC: voltage-gated sodium channel Declarations Ethics approval and consent to participate No specific permits were required for this study. The study did not involve endangered or protected species. Consent for publication Not applicable. Availability of data and materials All data generated or analyzed during this study are included in this published article. The newly generated sequences were submitted to the GenBank database under the accession numbers MN997310–MN997399. Competing interests The authors declare that they have no competing interests. Funding The Special Foundation of Basic Science and Technology Resources Survey of Ministry of Science and Technology of China (grant no. 2017FY101200), the Project of Basic Platform of National Science and Technology Resources of the Ministry of Sciences and Technology of China (grant no. TDRC-2019-194-30). Author’s contributions The paper was conceived Tambo Ernest, Yuan Fang, Emad I.M. Khater, Yi Zhang. The investigation was conducted by Yuan Fang, Emad I.M. Khater, Tambo Earnest, Data curation was performed by Tambo Ernest, Emad I.M. Khater for mosquito collection and providing reagents and materials, Yuan Fang for conducting molecular work and phylogenetic and statistical analyses, and Jing-Bo Xue for drawing map of mosquito collection sites. Funding acquisition: Yi Zhang, Tambo Ernest, Emad I.M. Khater and Xiao-Nong Zhou. Yuan Fang, Tambo Ernest and Yi Zhang wrote the first draft and Yuan Fang, Tambo Ernest, Yi Zhang, Jing-Bo Xue, Emad I.M. Khater reviewed and edited Acknowledgments Not applicable. References Sayono S, Hidayati APN, Fahri S, Sumanto D, Dharmana E, Hadisaputro S, et al. Distribution of voltage-gated sodium channel ( Nav ) alleles among the Aedes aegypti populations in Central Java Province and its association with resistance to pyrethroid insecticides. PLoS One. 2016;11:e0150577. Liu H, Xie L, Cheng P, Xu J, Huang X, Wang H, et al. Trends in insecticide resistance in Culex pipiens pallens over 20 years in Shandong, China. Parasite Vector. 2019;12:167. Moyes CL, Vontas J, Martins AJ, Ng LC, Koou SY, Dusfour I, et al. Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans. PLoS Neglect Trop Dis. 2017;11:e0005625. Brengues C, Hawkes NJ, Chandre F, Mccarroll L, Duchon S, Guillet P, et al. 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World malaria report 2019: Geneva: WHO; 2019. Balkew M, Ibrahim M, Koekemoer LL, Brooke BD, Engers H, Aseffa A, et al. Insecticide resistance in Anopheles arabiensis (Diptera: Culicidae) from villages in central, northern and south west Ethiopia and detection of kdr mutation. Parasit Vectors. 2010;3:40. Weaver SC, Charlier C, Vasilakis N, Lecuit M. Zika, chikungunya, and other emerging vector-borne viral diseases. Annu Rev Med. 2018;69:395–408. WHO. Test procedures for insecticide resistance monitoring in malaria vector mosquitoes. Geneva: WHO2013. Jones CM, Machin C, Mohammed K, Majambere S, Ali AS, Khatib BO, et al. Insecticide resistance in Culex quinquefasciatus from Zanzibar: implications for vector control programmes. Parasit Vectors. 2012;5:78. Tables Table 1. Frequency (percentage) of triple-locus kdr genotypes, shown as amino acid codon number 989, 1016 and 1534 in the voltage-gated sodium channel gene ( VSGC ) of Aedes aegypti collected from Jeddah, Saudi Arabia. Genotype SVF/PGF SVF/SVC SVC/SVC PGF/PGF PGF/PGC PGC/PGC No. 1 1 5 3 9 1 Percentage (%) 5.00 5.00 25.00 15.00 45.00 5.00 Table 2. Frequency (percentage) of point mutations (S989P, V1016G, F1534C) and intron types identified in the voltage-gated sodium channel gene ( VSGC ) of Aedes aegypti collected from Jeddah, Saudi Arabia. Codon 989 1016 1534 Intron type between exons 20 and 21 Allele type S P V G F C Type A Type B No. 13 27 13 27 18 22 28 12 Percentage (%) 32.50 67.50 32.50 67.50 45.00 55.00 70.00 30.00 Genotype SS SP PP VV VG GG FF FC CC Type A Type B No. 6 1 13 6 1 13 4 10 6 14 6 Percentage (%) 30.00 5.00 65.00 30.00 5.00 65.00 20.00 50.00 30.00 70.00 30.00 Table 3. Chi square analysis of the linkage between point mutations and type A and B introns located between exons 20 and 21 on voltage-gated sodium channel gene ( VGSC ) reported in Aedes aegypti from Jeddah, Saudi Arabia and homologous sequences retrieved from the GenBank from other geographic regions. χ 2 Df P 1016 982 Jeddah 22.000 2 0.000 989 Jeddah 44.000 4 0.000 Global 49.910 4 0.000 1534 Jeddah 15.402 4 0.004 Global 16.297 4 0.003 Intron type between exons 20 and 21 1016 Jeddah 22.000 2 0.000 Global 34.333 2 0.000 1534 Jeddah 11.746 2 0.003 Global 18.005 2 0.000 Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-35782","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":2895751,"identity":"a4d47a3d-8a08-4607-82e5-944f9612d3bb","order_by":0,"name":"Yuan Fang","email":"","orcid":"","institution":"National Institute of Parasitic Diseases","correspondingAuthor":false,"prefix":"","firstName":"Yuan","middleName":"","lastName":"Fang","suffix":""},{"id":2895752,"identity":"aabea8b4-6a90-4cda-8917-7ed62b8ba1a1","order_by":1,"name":"Tambo Ernest","email":"","orcid":"","institution":"Municipality of Jeddah Governorate","correspondingAuthor":false,"prefix":"","firstName":"Tambo","middleName":"","lastName":"Ernest","suffix":""},{"id":2895753,"identity":"8a0da762-3b9f-4926-a8f4-9fe3d6710f98","order_by":2,"name":"Jing-Bo Xue","email":"","orcid":"","institution":"National Institute of Parasitic Diseases","correspondingAuthor":false,"prefix":"","firstName":"Jing-Bo","middleName":"","lastName":"Xue","suffix":""},{"id":2895754,"identity":"0897df41-f74e-4b9b-ac1b-5d6496d96712","order_by":3,"name":"Yi Zhang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA9klEQVRIiWNgGAWjYDACCQgpx8AMZh/gQQgS0GJMshaGxAYI+wCyIHYgP7v52cOvbRbp29l5D95gqLkjY85++OGNHwx2ebi0MM45Zm4s2yaRu7OZL9mC4dgzHsueNGPLHobkYlxamCUSzKQlgVo2HOYxk2BsOMxjcCCHTRroQrBTsQE2ifRvIC3pBnAt59/g18IjkWMm+bFNIgGh5QYBWyQkcsqkGc5JGG44DPRLwjGQlmdAvxgk49QiPyN9m+SPsjp5g/NnD974UHPY3uB8MjDEKuxwagEHAS8b2I0MDAlwMQM86oGA8ccfqJZRMApGwSgYBdgAADzGUhkidYBqAAAAAElFTkSuQmCC","orcid":"","institution":"National Institute of Parasitic Diseases","correspondingAuthor":true,"prefix":"","firstName":"Yi","middleName":"","lastName":"Zhang","suffix":""},{"id":2895755,"identity":"89a93f60-7640-49cc-b27b-0fbbd0033d73","order_by":4,"name":"Xiao-Nong Zhou","email":"","orcid":"","institution":"National Institute of Parasitic Diseases","correspondingAuthor":false,"prefix":"","firstName":"Xiao-Nong","middleName":"","lastName":"Zhou","suffix":""},{"id":2895756,"identity":"9fb4f1eb-7d06-402b-a050-b9c9cb434010","order_by":5,"name":"Emad I.M. Khater","email":"","orcid":"","institution":"Ain Shams University","correspondingAuthor":false,"prefix":"","firstName":"Emad","middleName":"I.M.","lastName":"Khater","suffix":""}],"badges":[],"createdAt":"2020-06-15 13:12:55","currentVersionCode":2,"declarations":"","doi":"10.21203/rs.3.rs-35782/v2","doiUrl":"https://doi.org/10.21203/rs.3.rs-35782/v2","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":2789620,"identity":"ba36082e-867b-4613-9cc3-ee0c27263b94","added_by":"auto","created_at":"2020-10-05 20:36:29","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":43892,"visible":true,"origin":"","legend":"Map of mosquito collection sites for detection of target site mutations on insecticide resistance genes in 2016 in Jeddah, Saudi Arabia. Squares represent collection sites of Aedes aegypti, triangles represent sites of Culex quinquefasciatus.","description":"","filename":"Fig1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-35782/v2/Fig1.jpg"},{"id":2789621,"identity":"07339fd4-df4e-4cca-9bbd-25f69b15e2e1","added_by":"auto","created_at":"2020-10-05 20:36:29","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":37900,"visible":true,"origin":"","legend":"Maximum likelihood phylogeny of introns between exons 20 and 21 of the voltage-gated sodium channel gene of Aedes aegypti, constructed by 158 sequences under the TVM+ G model. The GenBank accession number, collection country/province and year of each sequence are noted. The sequences obtained in this study are marked in red. The numbers above the branch represent the bootstrap support of the maximum likelihood, neighbor-joining, and Bayesian analyses, respectively, based on 1000 replicates. The scale-bar indicates 0.01 nucleotide substitutions per site. Dark grey and light grey indicate the type A and B of the introns located between exons 20 and 21.","description":"","filename":"Fig2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-35782/v2/Fig2.jpg"},{"id":15669626,"identity":"b278bf72-d6a9-4a9c-a78d-a8ca4c33a1b8","added_by":"auto","created_at":"2021-11-18 13:54:27","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":517050,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-35782/v2/c2278ad3-7b70-483d-9d51-ddaaade38564.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eMolecular analysis of targeted insecticide resistance gene mutations in field \u003cem\u003eAedes aegypti \u003c/em\u003eand \u003cem\u003eCulex quinquefasciatus\u003c/em\u003e mosquito vectors of arboviruses from Saudi Arabia\u003c/p\u003e","fulltext":[{"header":"Introduction","content":"\u003cp\u003eMosquitoes-borne diseases, including dengue, malaria, West Nile, Japanese encephalitis, Rift Valley fever, Chikungunya, Zika, and other emerging arboviral diseases are prevalent and are enormous threat to public health worldwide. Intensive international trade and travel, influenced by global economic integration, accelerated the spread of mosquito-borne viruses, resulting in occasional disease outbreaks even in previously endemic or disease-free areas. Since no protective vaccines and effective drugs are available for majority of these infectious diseases, the strategies on reducing the risk of transmission is mainly focused on mosquito vector control. Vector control has been relying heavily on insecticides application to reduce mosquito larval and adult populations in their natural habitats as well as personal protection using anti-mosquito repellents [\u003ca href=\"#_ENREF_1\"\u003e1\u003c/a\u003e]. Selection pressure on insecticide target sites of mosquito vectors, resulting in the occurrence and developing of single or multiple-site mutations in the responsible genes have been reported and subsequently detected globally in major mosquito vector species belonging to the genera \u003cem\u003eAnopheles\u003c/em\u003e, \u003cem\u003eAedes\u003c/em\u003e and \u003cem\u003eCulex\u003c/em\u003e in the last few decades [\u003ca href=\"#_ENREF_2\"\u003e2\u003c/a\u003e, \u003ca href=\"#_ENREF_3\"\u003e3\u003c/a\u003e]. Increased insecticide target site insensitivity due to gene point mutations, and the increased or elevated activity of insecticide detoxifying enzymes are two of the major mechanisms evolved and developed by mosquito vectors for surviving the lethal effect of insecticides [\u003ca href=\"#_ENREF_4\"\u003e4\u003c/a\u003e, \u003ca href=\"#_ENREF_5\"\u003e5\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eAt least four vector-borne viruses, including dengue virus (DENV), Alkhurma hemorrhagic fever virus, Rift Valley fever virus, and Crimean-Congo hemorrhagic fever virus have been circulating in Saudi Arabia [\u003ca href=\"#_ENREF_6\"\u003e6\u003c/a\u003e]. The most important of them is dengue, with most of the disease burden and severity, mainly prevalent in the western and southwestern regions in Jeddah and Makkah, of Saudi Arabia [\u003ca href=\"#_ENREF_7\"\u003e7-9\u003c/a\u003e]. A total of 5,345 confirmed dengue cases were reported in 2018, with incidence rate of 16/100,000 population, with most of cases (92.46%, n = 4,942) in Jeddah and 3.05% (n = 163) in Makkah, according to the Ministry of Health of Saudi Arabia recent reports [\u003ca href=\"#_ENREF_10\"\u003e10\u003c/a\u003e]. Jeddah is the financial and diplomatic center in Saudi Arabia, and is the major point-of-entry to the Holy city of Makkah (only 80 km SE of Jeddah) for millions of visitors of perennial religious rituals for pilgrimage (Hajj) and Umrah as well as workers and other visitors. \u003cem\u003eAedes aegypti \u003c/em\u003e(Linnaeus, 1762), the major mosquito vector of dengue, chikungunya and Zika, and \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e Say, 1823, vector of West Nile, Rift Valley fever and Japanese encephalitis, are two dominant mosquitoes in Jeddah [\u003ca href=\"#_ENREF_11\"\u003e11\u003c/a\u003e, \u003ca href=\"#_ENREF_12\"\u003e12\u003c/a\u003e]. Although there have been no reports of West Nile virus outbreaks in Jeddah, it has been reported in countries neighbouring Saudi Arabia, such as Pakistan, Iran, Egypt and Israel [\u003ca href=\"#_ENREF_13\"\u003e13-15\u003c/a\u003e]. Since the distribution of the two vectors\u003cem\u003e Ae. aegypti\u003c/em\u003e and \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e are widespread and both are domestic and endophagic species, there is an ever-present risk of arboviral disease outbreaks. In our previous paper, DENV-2 virus and four strains of insect-specific flaviviruses, including one of cell-fusing agent virus (CFAV) and three of Phlebotomus-associated flavivirus (PAFV) were detected in pools of \u003cem\u003eAe. aegypti\u003c/em\u003e, and 10 strains of Culex flavivirus (CxFV) in pools of \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e population from Jeddah[\u003ca href=\"#_ENREF_16\"\u003e16\u003c/a\u003e]. Meanwhile, the two species suffered from extensive and long-time insecticide exposure for routine mosquito control operations using larvicides spraying of their natural breeding habitats and indoor residual spraying (IRS) using adulticides, resulting in developing of insecticide resistance, which subsequently reduced the efficacy of insecticide treatments [\u003ca href=\"#_ENREF_7\"\u003e7\u003c/a\u003e, \u003ca href=\"#_ENREF_9\"\u003e9\u003c/a\u003e, \u003ca href=\"#_ENREF_15\"\u003e15\u003c/a\u003e, \u003ca href=\"#_ENREF_17\"\u003e17\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eThe extensive use of insecticide result in the occurrence and development of insecticide resistance in local populations. Investigations on the pattern of pesticide uses in integrated vector management (IVM) programs revealed that chemical insecticides control has been the last resort and major control approach of mosquito vector and public health pest in the Kingdom. Insecticide application methods were mainly space spraying (e.g., fogging, ULV), larviciding residual spraying. Over a period of 20 years, an overall volume of insecticides used in IVM were 64,000 liters, 140,000 liters and 268,868 liters (mainly pyrethroid and organophosphate insecticides) from 2002\u0026ndash;2006, 2007\u0026ndash;2012 and 2012 to date by the government and others private contractors in routine mosquito control operations, respectively. The pyrethroid group (mainly deltamethrin, cypermethrin, and cyfluthrin), sporadically the organophosphates pirimiphos-methyl and diazinon, have been the most common insecticides used in different commercial formulations over the last 20 years in the Kingdom.\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eAedes aegypti\u003c/em\u003e populations from Jeddah and Makkah showed high resistance to permethrin, deltamethrin, bendiocarb, and were tolerant to fenitrothion, based on conventional insecticide bioassays performed on adult mosquito samples collected in 2015 [\u003ca href=\"#_ENREF_18\"\u003e18\u003c/a\u003e]. Noteworthy, in a tenf-year period, the mortalities to pyrethroids decreased from 75\u0026ndash;93% in 2008 to \u0026lt; 20% in 2017 in the \u003cem\u003eAe. aegypti\u003c/em\u003e population in Makkah [\u003ca href=\"#_ENREF_18\"\u003e18\u003c/a\u003e]. In addition, the Jazan population was only sensitive to cyfluthrin, tolerant to deltamethrin, permethrin, fenitrothion, and resistant to Lambda-cyhalothrin, DDT, and bendiocarb [\u003ca href=\"#_ENREF_19\"\u003e19\u003c/a\u003e]. Whereas, resistance to DDT, permethrin and deltamethrin in \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e from Jeddah developed as early as 1984 [\u003ca href=\"#_ENREF_20\"\u003e20\u003c/a\u003e]. However, to our knowledge, no relevant record reported on insecticide resistance in \u003cem\u003eCx. quinquefasciatus \u003c/em\u003efrom Saudi Arabia after then, especially in field-caught individuals.\u003c/p\u003e\n\u003cp\u003eThe most prevalent insecticide resistance gene mutation is the knockdown resistance (\u003cem\u003ekdr\u003c/em\u003e) mutation on the para voltage sodium channel gene (\u003cem\u003eVGSC\u003c/em\u003e). The mutation sites on the amino acid 1014 position on \u003cem\u003eVGSC\u003c/em\u003e IIS for both \u003cem\u003eAnopheles\u003c/em\u003e and \u003cem\u003eCulex\u003c/em\u003e, and 1016 position for \u003cem\u003eAedes\u003c/em\u003e, were well confirmed to reduce the mosquito sensitivity to pyrethroid, and the organochlorine DDT due to cross-resistance effect [\u003ca href=\"#_ENREF_21\"\u003e21-23\u003c/a\u003e]. In specific, several point mutation sites on \u003cem\u003eVGSC \u003c/em\u003eare speculated to get involved in pyrethroid and DDT resistance in \u003cem\u003eAe. aegypti\u003c/em\u003e, including G923V, L982W, S989P, M1011I/G, V1016G, F1269C in domain II, and T1520I, F1552C, F1534C, D1763Y in domain III of \u003cem\u003eVGSC \u003c/em\u003e[\u003ca href=\"#_ENREF_1\"\u003e1\u003c/a\u003e, \u003ca href=\"#_ENREF_24\"\u003e24-26\u003c/a\u003e]. Among them, two point mutations, V1016G and F1534C that confer resistance to pyrethroids and DDT, are the most widespread [\u003ca href=\"#_ENREF_27\"\u003e27-32\u003c/a\u003e]. Four non-synonymous (A99S, L1014F/S/C, V1016G, W1594R) and six synonymous, (L884L, G923G, A1262A, D1266D, P1270P, G1754G) mutations were found to be linked with the levels of permethrin resistance in \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e populations [\u003ca href=\"#_ENREF_33\"\u003e33-35\u003c/a\u003e]. In addition, the replacement of the amino glycine (Gly, G) with serine (Ser, S) on position 119 of acetylcholinesterase 1 (\u003cem\u003eace-1\u003c/em\u003e) was found to be associated with mosquito resistance to organophosphate and carbamate insecticide groups [\u003ca href=\"#_ENREF_36\"\u003e36\u003c/a\u003e]. The G119S mutation was commonly detected in \u003cem\u003eAnopheles\u003c/em\u003e and \u003cem\u003eCulex\u003c/em\u003e, while seldomly recorded in \u003cem\u003eAedes \u003c/em\u003especies [\u003ca href=\"#_ENREF_1\"\u003e1\u003c/a\u003e]. In Saudi Arabia, S989P, V1016G and F1534C mutations on \u003cem\u003eVGSC\u003c/em\u003e were reported in \u003cem\u003eAe. aegypti\u003c/em\u003e samples collected from Jeddah and Makkah in 2015 [\u003ca href=\"#_ENREF_18\"\u003e18\u003c/a\u003e]. It is the first on insecticide resistance gene detection in \u003cem\u003eAe. aegypti\u003c/em\u003e populations from Saudi Arabia. However, little is documented on the trend and impact of insecticides application in IVM programs implementation nearly for 20 years (1995\u0026ndash;2016) on vector-borne disease threats and epidemics prevention and control in the Kingdom of Saudi Arabia. Additionally, point mutations in the \u003cem\u003eVGSC\u003c/em\u003e gene of \u003cem\u003eAe. aegypti\u003c/em\u003e populations in Jeddah has not been fully characterized, especially the types of introns between exons 20 and 21, as well as whether the \u003cem\u003eace-1 \u003c/em\u003emutations in Jeddah \u003cem\u003eAe. aegypti\u003c/em\u003e populations. In \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e, the V1016G mutation was detected in the JPP-B strain, originated from Jeddah and selected by permethrin for 20 consecutive generations [\u003ca href=\"#_ENREF_20\"\u003e20\u003c/a\u003e, \u003ca href=\"#_ENREF_34\"\u003e34\u003c/a\u003e]. This mutation has not been reported in other populations of \u003cem\u003eCx. pipiens \u003c/em\u003ecomplex [\u003ca href=\"#_ENREF_37\"\u003e37\u003c/a\u003e]. To the best of our knowledge since the first studies performed in the mid of 1980s, no study on insecticide resistance gene(s) in \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e populations have been reported in the so far in Saudi Arabia. Thus, currently, there are limited information on insecticide resistance gene mutations in \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e against chemical insecticides in Jeddah. For the bioassay test requires numerous live mosquitoes and time-consuming [\u003ca href=\"#_ENREF_38\"\u003e38\u003c/a\u003e]. Early detection and characterization of target mutations of insecticide resistance genes, as potentially valuable molecular markers, can provide a reference to estimate the local insecticide resistance level for monitoring, planning and evaluation of vector control strategy [\u003ca href=\"#_ENREF_1\"\u003e1\u003c/a\u003e, \u003ca href=\"#_ENREF_39\"\u003e39\u003c/a\u003e, \u003ca href=\"#_ENREF_40\"\u003e40\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eThis study aimed to determine the status of insecticide resistance in \u003cem\u003eAe. aegypti\u003c/em\u003e and \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e mosquito vectors, by mapping the point mutations associated with \u003cem\u003ekdr \u003c/em\u003emutations and the phylogeny of the introns between the exons 20 and 21 on \u003cem\u003eVGSC\u003c/em\u003e, and \u003cem\u003eace-1\u003c/em\u003e. The results of our study will be very important for the early-detection of resistance development and in guiding IVM and insecticide application policies for the prevention and control of mosquito-borne diseases in Saudi Arabia.\u003c/p\u003e"},{"header":"Materials And Methods","content":"\u003cp\u003e\u003cstrong\u003eMosquito Collection\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eA total of 25 \u003cem\u003eAe. aegypti\u003c/em\u003e and 24 \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e female mosquitoes were analyzed, representing different districts and municipalities of Jeddah Governorate. These mosquitoes were collected during routine mosquito surveillance in 2016. Collection sites for detection of target site mutations on insecticide resistance genes are shown in Fig. 1, and a map was generated using ArcGIS 10.1 ArcMap software (ESRI, Redlands, CA, USA). The species name, geographic information (including the latitude and longitude information), and GenBank accession numbers of each randomly selected samples are shown in Additional file 1.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDNA extraction and target gene mutation sequencing\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFor DNA extraction, individual mosquito samples, each was homogenized in a mixer mill (Jingxin, Shanghai, China) in 250 \u0026mu;l of ATL (Qiagen, Hilden, Germany) and 20 \u0026mu;l of proteinase K (Qiagen, Hilden, Germany) with two 3-mm steel balls were added to each tube. The homogenates were incubated at 56 \u0026deg;C overnight in an oscillating thermo-block. The homogenate tubes were then centrifuged at 1000 \u0026times; rpm for 3 min at room temperature. A 200 \u0026mu;l aliquot of supernatant from each homogenate was added to the Roche\u003csup\u003e\u0026reg;\u003c/sup\u003e MagNA Pure 96 sample plate, and placed into to the MagNA Pure 96 System for automated DNA extraction (MagNA Pure 96 DNA and Viral NA Small Volume Kit, Roche, Basel, Switzerland) according to the manufacturer\u0026rsquo;s instructions DNA was eluted in 100 \u0026mu;l of buffer solution.\u003c/p\u003e\n\u003cp\u003eFor \u003cem\u003eAe. aegypti\u003c/em\u003e, the primer set IIS5-6(3)F and IIS5-6(3)R were used to amplify the IIS6 domain of the \u003cem\u003eVGSC\u003c/em\u003e, spanning the codons 989, 1011, 1016, and the introns between exons 20 and 21; while the codon 1534 domain IIIS6 was amplified by the primer set AaNa31F and AaNa31R [\u003ca href=\"#_ENREF_41\"\u003e41\u003c/a\u003e]. The \u003cem\u003eace-1\u003c/em\u003e gene region spanning the position 119 was amplified by the primer set Ace-1F and Ace-1R [\u003ca href=\"#_ENREF_42\"\u003e42\u003c/a\u003e]. For \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e, the \u003cem\u003eVGSC\u003c/em\u003e region spanning the 1014 codon was amplified by the primer set Cq1 and Cq2 [\u003ca href=\"#_ENREF_43\"\u003e43\u003c/a\u003e]. The fragment containing the \u003cem\u003eace-1\u003c/em\u003e mutation was amplified by the primer set Moustdir and Moustrev [\u003ca href=\"#_ENREF_44\"\u003e44\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eThe PCR amplification products were separated on 1.5% agarose gel electrophoresis, purified, and sequenced in both directions by Sangon Biotech (Shanghai, China). The peak spectrum (chromatogram) of the sequencing data was determined using Bio-edit v.7.2.6 [\u003ca href=\"#_ENREF_45\"\u003e45\u003c/a\u003e]. The allelic information for certain ambiguous sequences, cloning and plasmid sequencing were performed for confirmation.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhylogenetic analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe obtained \u003cem\u003eVGSC\u003c/em\u003e IIS6 fragments spanning the introns flanked by exons 20 and 21 were aligned with available homologous sequences retrieved from GenBank using ClustalW2 [\u003ca href=\"#_ENREF_46\"\u003e46\u003c/a\u003e] with default settings, which were manually adjusted when was necessary. Based on the Akaike information criterion, the best-fit model for the alignment was determined using Model test 3.7 [\u003ca href=\"#_ENREF_47\"\u003e47\u003c/a\u003e], in cooperation with PAUP* v.4.0b10 [\u003ca href=\"#_ENREF_48\"\u003e48\u003c/a\u003e]. Consequently, calculation of the maximum likelihood (ML) and Bayesian likelihood trees were completed under the TVM+ G model for introns between exons 20 and 21 on the \u003cem\u003eVGSC\u003c/em\u003e. Both the ML and neighbour-joining trees were constructed using MEGA v.7.0 software [\u003ca href=\"#_ENREF_49\"\u003e49\u003c/a\u003e], with 1,000 bootstrap replicates. The Bayesian tree was built with MrBayes v3.2.6 [\u003ca href=\"#_ENREF_50\"\u003e50\u003c/a\u003e] on the CIPRES portal (\u003ca href=\"http://www.phylo.org/\"\u003ewww.phylo.org/\u003c/a\u003e) [\u003ca href=\"#_ENREF_51\"\u003e51\u003c/a\u003e], run for 10 million generations, with the first 25% generations discarded as burn-in. The trees were visualised using Figtree v1.4.2 (\u003ca href=\"http://tree.bio.ed.ac.uk/software/figtree/\"\u003ehttp://tree.bio.ed.ac.uk/software/figtree/\u003c/a\u003e). Both intra- and inter-specific divergences of introns spanning between exons 20 and 21 were calculated using K2P distance model [\u003ca href=\"#_ENREF_52\"\u003e52\u003c/a\u003e] in MEGA v.7.0 .\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eStatistical analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePearson\u0026rsquo;s Chi-squared test was applied to analyze associations among mutation codons and type of introns between exons 20 and 21 on \u003cem\u003eVGSC\u003c/em\u003e. The tests were performed in SPSS v.20 (IBM Corp., Armonk, NY, USA).\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003eMapping of the target resistance-associated genes in \u003cem\u003eAe. aegypti\u003c/em\u003e and \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe target resistance-associated genes successfully amplified from a total of 20 \u003cem\u003eAe. aegypti\u003c/em\u003e and 21 \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e. The sequences originally obtained in this study have been deposited in the GenBank under the accession numbers MN997310\u0026ndash;MN997399. Three non-synonymous \u003cem\u003eVGSC \u003c/em\u003emutation alleles, S989P, V1016G and F1534C were identified from specimens of wild \u003cem\u003eAe. aegypti\u003c/em\u003e. The presence of a silent mutation (TTG to TTA) at position (Leu, L) 982 (14/20) was found. No I1011M and F1552C mutations were identified. In addition, two types of introns between exons 20 and 21, type A (250 bp) and type B (234 bp) were identified.\u003c/p\u003e\n\u003cp\u003eSix genotypes of the combination 989, 1016, and 1534 positions were documented including SVF/PGF, SVF/SVC, SVC/SVC, PGF/PGF, PGF/PGC, PGC/PGC (Table 1). Among them, PGF/PGC was the dominant genotype, detected in 45% of the examined samples, followed by the SVC/SVC genotype with 25%. Genotypes of SVF/PGF, SVF/SVC, and PGC/PGC were observed less frequently. Allele frequencies are shown in Table 2. The V1016G mutation seems to be more widely distributed with high frequency (67.50%) in Jeddah, than that of the F1534C mutation (55.00%). Both TTG982TTA (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 22.000, df = 2, P \u0026lt; 0.001), and S989P (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 44.000, df = 4, P \u0026lt; 0.001) co-occur with the presence of V1016G. All TTG982TTA, and S989P coexist with the haplotype harboring the V1016G mutation. In individuals carrying homozygous F1534C mutation in the IIIS6 region, no coexistence of other mutations on 982, 989, and 1016 codons in IIS6 domain was observed.\u003c/p\u003e\n\u003cp\u003eThe type A intron between exons 20 and 21 was strongly linked to the V1016G mutation. The haplotype with type A intron was in association with V1016G mutation (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 22.00, df = 2, P \u0026lt; 0.001) and wild-type F1534 (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 11.75, df = 2, P = 0.003). By contrast, the F1534C mutation was strongly linked with the type B intron with all of the SVC/SVC genotype linked with the type B intron. The allele type on 1016 position was highly correlated with the other two mutations, 989 and 1534, especially the 989 position (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 44.00, df = 4, P \u0026lt; 0.001). All homozygous V1016G mutations linked with homozygous S989P mutation, V1016G heterozygous mutation linked with heterozygous S989P mutation, whereas wild-type of V1016 is linked with the S989. Individuals possessing homozygous wild-type V1016 harbored homozygous (5/6) or heterozygous (1/6) of the F1534C mutation, however, no wild-type homozygous F1534 coexisted.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhylogenetic analysis of intron types identified in the voltage-gated sodium channel gene (\u003cem\u003eVSGC\u003c/em\u003e) of \u003cem\u003eAedes aegypti\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe intron located between exons 20 and 21 on \u003cem\u003eVGSC\u003c/em\u003e identified in our study and those retrieved from the GenBank were compared and used to calculate the evolution of the \u003cem\u003ekdr\u003c/em\u003e mutations. The studied 158 sequences can be clearly divided into two clades with high bootstrap values, one clade contained the sequences possessing type A intron, while the second clade contained those sequences having type B intron (Fig. 2). The intra-group genetic distances of type A and type B introns were 0.001 and 0.049, respectively, whereas the inter-group was 0.355. The two intron types are detected in four continents, Africa, Asia and Latin and North America, suggesting that they were distributed paraphyletically. In Latin America (Brazil, El-Salvador, Guatemala) and North America (USA: California and Hawaii), the ratio of type A to type B intron were 5:1 and 4:0, respectively; while in Asia (China, India, Indonesia, Malaysia, Myanmar, Philippines, Saudi Arabia, Singapore, Sri Lanka, Thailand, Viet Nam), the ratio was 88:37; and in Africa (Ghana, Kenya, Malawi, Nigeria, Zambia, Zimbabwe), the ratio was 4:17. In addition, for global analysis the associations among mutation codons and type of introns between exons 20 and 21 on \u003cem\u003eVGSC\u003c/em\u003e, we added specific 989, 1016 and 1534 allele types of those sequences, available from other studies. The results of analysis of the associations between the three codons and the intron types reported, showed that the allele type of 1016 position was more related to the 989 position (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 49.910, df = 4, P \u0026lt; 0.001) than to the 1534 position (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 16.297, df = 4, P = 0.003). While the intron polymorphism was more correlated to the 1016 position (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 34.333, df = 2, P \u0026lt; 0.001) than the 1534 position (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 18.005, df = 2, P \u0026lt; 0.001) (Table 3).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePoint mutations and polymorphisms on\u003cem\u003e VGSC\u003c/em\u003e and \u003cem\u003eace-1\u003c/em\u003e in \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe majority of the entire successfully sequenced partial \u003cem\u003eVGSC\u003c/em\u003e gene with \u003cem\u003ekdr\u003c/em\u003e mutation in \u003cem\u003eCx. quinquefasciatus \u003c/em\u003ewas the homozygous genotype TTT/TTT (90.48%), except two of them was the heterozygous genotype TTT/TTA.\u003c/p\u003e\n\u003cp\u003eAll the detected \u003cem\u003eace-1\u003c/em\u003e gene was the heterozygous genotype GGC/AGC.\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThe application of IRS and larvicides to reduce and control populations abundance of\u003cem\u003e Aedes\u003c/em\u003e, \u003cem\u003eCulex\u003c/em\u003e and \u003cem\u003eAnopheles\u003c/em\u003e mosquitoes responsible for dengue, Rift Valley, West Nile and malaria infections witnessed significant decline in the years 1970s and 1980s. With the persistent mosquito-borne disease foci and endemicity in the major Saudi cities, Jeddah, Makkah, Madinah, Aseer and Jazan, in the South-Western and southern borders regions, renewed governments efforts ushered in enhanced pest surveillance coupled with integrated vector control programs implementation showed significant decline of total national cases from 2000 till now. However, as a side-effect, insecticide resistance in local populations has occurred and developed.\u003c/p\u003e\n\u003cp\u003eIn the current study, three non-synonymous point mutations on 989, 1016 and 1534 positions and type A and B introns on \u003cem\u003eVGSC \u003c/em\u003ewere reported detected in field-caught \u003cem\u003eAe. aegypti\u003c/em\u003e mosquito samples in 2016 from Jeddah, Saudi Arabia. No samples that harbored the wild-type at the three mutation loci simultaneously were identified. The I1011M/V mutation was absent in Jeddah, even though this substitution of isoleucine on 1011 position is prevalent in Latin America countries [\u003ca href=\"#_ENREF_39\"\u003e39\u003c/a\u003e, \u003ca href=\"#_ENREF_41\"\u003e41\u003c/a\u003e, \u003ca href=\"#_ENREF_53\"\u003e53-55\u003c/a\u003e], and spread in Thailand and Vietnam but at low frequencies [\u003ca href=\"#_ENREF_56\"\u003e56\u003c/a\u003e, \u003ca href=\"#_ENREF_57\"\u003e57\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eSo far, to our knowledge, Saudi Arabia is the place with the most variation in \u003cem\u003ekdr\u003c/em\u003e mutations associated with high resistance to pyrethroids in the Middle East countries, probably due to long-term usage of high doses of pyrethroids in vector control programs in and all major cities, especially in Jeddah since the first dengue epidemics in 1994. This is different from the reported \u003cem\u003ekdr\u003c/em\u003e polymorphisms of \u003cem\u003eAe. aegypti\u003c/em\u003e populations from India and Pakistan. High prevalence of the F1534C mutation, and a low frequency of I1011M/V and V1016I mutations, with the absence of the 989P allele, were observed in India [\u003ca href=\"#_ENREF_25\"\u003e25\u003c/a\u003e]. In Pakistan, all the studied mosquito samples harbored the homozygous F1534C mutation, whereas no mutation on the 1016 position has been identified yet [\u003ca href=\"#_ENREF_58\"\u003e58\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eThe percentage of homozygous V1016G and S989P mutations co-occurrence was high (65.0%) in Jeddah. The V1016G mutation plays a critical role in \u003cem\u003ekdr\u003c/em\u003e resistance against pyrethroid compounds [\u003ca href=\"#_ENREF_22\"\u003e22\u003c/a\u003e, \u003ca href=\"#_ENREF_23\"\u003e23\u003c/a\u003e, \u003ca href=\"#_ENREF_58\"\u003e58\u003c/a\u003e]. Linkage association has been observed between the 989 and 1016 loci in \u003cem\u003eAe. aegypti\u003c/em\u003e populations of Saudi Arabia [\u003ca href=\"#_ENREF_18\"\u003e18\u003c/a\u003e], Myanmar [\u003ca href=\"#_ENREF_59\"\u003e59\u003c/a\u003e], Thailand [\u003ca href=\"#_ENREF_60\"\u003e60\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eIt is speculated the S989P may serve as a compensatory mutation to the V1016G, to reduce the fitness cost on the mosquito [\u003ca href=\"#_ENREF_22\"\u003e22\u003c/a\u003e] and strengthen the response of V1016G to permethrin and deltamethrin [\u003ca href=\"#_ENREF_61\"\u003e61\u003c/a\u003e]. Additionally, double homozygous mutations at positions 989 and 1016 combined with either wild-type or heterozygous mutation at 1534 position were common in Jeddah\u0026rsquo;s population, in agreement with the speculation that the 1016 and 1534 loci were not independent for linkage disequilibrium analysis [\u003ca href=\"#_ENREF_62\"\u003e62\u003c/a\u003e]. Further, the genotype with the triple mutation haplotype predominated in the tested mosquito samples, with an occupation of 45% (PGC/PGF). Additionally, one individual possessing triple homozygous mutation on 989, 1016, and 1534 loci was found in this study. In field-collected mosquitoes, the triple homozygous mutation was in low frequency in the population [\u003ca href=\"#_ENREF_32\"\u003e32\u003c/a\u003e, \u003ca href=\"#_ENREF_58\"\u003e58\u003c/a\u003e, \u003ca href=\"#_ENREF_59\"\u003e59\u003c/a\u003e, \u003ca href=\"#_ENREF_63\"\u003e63\u003c/a\u003e, \u003ca href=\"#_ENREF_64\"\u003e64\u003c/a\u003e], probably due to fitness cost exerted on the mosquito by this triple mutation [\u003ca href=\"#_ENREF_61\"\u003e61\u003c/a\u003e, \u003ca href=\"#_ENREF_65\"\u003e65\u003c/a\u003e]. Whereas, the artificially-introduced triple mutation, using the \u003cem\u003eXenopus\u003c/em\u003e oocyte expression systems, appeared to confer a higher level of resistance to both permethrin and deltamethrin than those carrying the individual mutations, probably for synergistic effect of the combination of mutant alleles [\u003ca href=\"#_ENREF_22\"\u003e22\u003c/a\u003e, \u003ca href=\"#_ENREF_23\"\u003e23\u003c/a\u003e, \u003ca href=\"#_ENREF_61\"\u003e61\u003c/a\u003e]. Li et al., [\u003ca href=\"#_ENREF_30\"\u003e30\u003c/a\u003e] suggested that the F1534C mutation could possibly serve as a compensatory mutation for reducing the fitness cost on the mosquito induced by the V1016G mutation. Thus, whether the homozygous triple mutations on 989, 1016, and 1534 loci showing insecticide resistance in field mosquitoes is yet to be ascertained.\u003c/p\u003e\n\u003cp\u003eThe intron polymorphism of \u003cem\u003eVGSC \u003c/em\u003ecould serve as a marker to study the evolution of \u003cem\u003ekdr \u003c/em\u003emutations [\u003ca href=\"#_ENREF_38\"\u003e38\u003c/a\u003e, \u003ca href=\"#_ENREF_53\"\u003e53\u003c/a\u003e]. Based on sequence size, the introns located between exons 20 and 21 were classified into type A (250 pb) and type B (234 pb) [\u003ca href=\"#_ENREF_53\"\u003e53\u003c/a\u003e]. Analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 34.333, df = 2, P = 0.000), and 1534 (\u0026chi;\u003csup\u003e2\u003c/sup\u003e = 18.005, df = 2, P = 0.000) allele type. Further, all the individuals possessing the V1016G mutation, either heterozygous or homozygous harbored the type A intron, but not vice versa. This result is in contrast to Saavedra-Rodriguez et al.,\u0026rsquo;s [\u003ca href=\"#_ENREF_39\"\u003e39\u003c/a\u003e] suggestion that the V1016G mutation was distributed independently in haplotypes possessing either the type A or type B intron. Additionally, the global data analysis showed that the two intron types were distributed evenly in both heterozygous or homozygous F1534C mutation, but the homozygous wild-type F1534 was mainly associated with the type A intron, and not vice versa as well. In Jeddah, six of the examined mosquito individuals were harboring the homozygous F1534C mutation. Among them, five individuals with the SVC/SVC genotype possessed the type B intron, while the individual carrying the homozygous triple mutation (PGC/PGC) was coupled with the type A intron. In Taiwan Province, China, the F1534C was found to be strictly associated with the type B intron, and the V1016G mutation coexisted with the type A intron [\u003ca href=\"#_ENREF_38\"\u003e38\u003c/a\u003e]. By contrast, in Africa, the point mutation at 1534 site was found to be strongly linked with the type A intron in Ghana, but only one heterozygote point mutation V1016I was recorded, except the F1534C mutations [\u003ca href=\"#_ENREF_66\"\u003e66\u003c/a\u003e]. Additionally, V1016I was reported to locate at the haplotype possessing the type A intron, with no 1534 variation observed in Brazil [\u003ca href=\"#_ENREF_53\"\u003e53\u003c/a\u003e, \u003ca href=\"#_ENREF_67\"\u003e67\u003c/a\u003e]. The mutation at position 1016 is associated with resistance to both type I and II pyrethroids, while the F1534C allele is primarily associated with resistance to type I pyrethroids, and the V1016I mutation did not alter channel sensitivity to pyrethroids in \u003cem\u003eXenopus\u003c/em\u003e oocytes [\u003ca href=\"#_ENREF_22\"\u003e22\u003c/a\u003e, \u003ca href=\"#_ENREF_23\"\u003e23\u003c/a\u003e]. Based on the evidences above, we speculate that \u003cem\u003ekdr\u003c/em\u003e mutations incline to coexist with the type A intron. When a mutation occurred in either 1016 or 1534 positions, it would be linked to the type A intron. While in case of co-mutation, the type A intron distributes more frequently with the V1016G/I mutation rather than the mutation F1534C. It is probably due to balancing the fitness cost on the mosquito by increasing compensatory advantage or linkage disequilibrium [\u003ca href=\"#_ENREF_61\"\u003e61\u003c/a\u003e, \u003ca href=\"#_ENREF_68\"\u003e68\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eIt is speculated that the type B intron is the ancestral clade of clade 1, because that the \u003cem\u003eAe. aegypti\u003c/em\u003e was originally from Africa and the majority of African individuals belong to type B [\u003ca href=\"#_ENREF_66\"\u003e66\u003c/a\u003e, \u003ca href=\"#_ENREF_69\"\u003e69\u003c/a\u003e]. In view of the phylogenetic tree obtained with available global data of the intron sequences, 100%, 83.33% and 70.40% individuals from North America, Latin America and Asian countries respectively, were type A intron. By contrast, 73.91% of sequences from Africa belonged to the type B intron. According to our analyses above, the type A intron coexistence with \u003cem\u003ekdr\u003c/em\u003e mutations is common, especially the V1016G mutation; while the type B couples with the wild-type or F1534C mutation when the V1016G mutation is absent. This variable pattern of association, may reflect the history of insecticide treatment in different continents. The major disease burden of malaria is in Africa and South-East Asia [\u003ca href=\"#_ENREF_70\"\u003e70\u003c/a\u003e]. The mutation on 1534 locus may have arisen in \u003cem\u003eAedes\u003c/em\u003e species due to earlier or current use of DDT for larvicides spraying and IRS to control malaria since the middle of the last century [\u003ca href=\"#_ENREF_41\"\u003e41\u003c/a\u003e, \u003ca href=\"#_ENREF_71\"\u003e71\u003c/a\u003e]. Whereas both in Latin and North America countries, vector control is mainly focused on \u003cem\u003eAedes\u003c/em\u003e species (mainly \u003cem\u003eAe. aegypti\u003c/em\u003e and \u003cem\u003eAe. albopictus\u003c/em\u003e) vectors of dengue, Chikungunya, yellow fever, and Zika [\u003ca href=\"#_ENREF_72\"\u003e72\u003c/a\u003e]. For the domestic and endophagic habitats of \u003cem\u003eAe. aegypti\u003c/em\u003e, pyrethroid insecticides are widely and randomly used in household aerosol insecticides for public and personal protection, since that pyrethroids have the advantages of high efficacy against mosquito vectors, low mammalian toxicity and short residual action [\u003ca href=\"#_ENREF_73\"\u003e73\u003c/a\u003e]. In South Asia countries, the higher frequency of type A intron than that of type B may indicate different selective pressures resulting in different histories of intense and unrestricted insecticide usage. It is probably that first, the F1534C mutations occurred due to the extensive use of DDT or type I pyrethroids, as both insecticides have the same target site [\u003ca href=\"#_ENREF_41\"\u003e41\u003c/a\u003e], then V1016G has been selected by the subsequent and wide use of type II pyrethroids, as cypermethrin and deltamethrin [\u003ca href=\"#_ENREF_59\"\u003e59\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eBoth the non-synonymous mutation G119S (GGC/AGC) and silent mutation T506T on \u003cem\u003eace-1\u003c/em\u003e [\u003ca href=\"#_ENREF_42\"\u003e42\u003c/a\u003e] have not been detected in our sequences in \u003cem\u003eAe. aegypti\u003c/em\u003e samples from Jeddah. While it does not indicate that the \u003cem\u003eAe. aegypti\u003c/em\u003e population was completely susceptible to organophosphate and carbamate compounds, as both types of insecticides have the same target site. In bioassay tests, the \u003cem\u003eAe. aegypti\u003c/em\u003e population from Jeddah was tolerant to the fenitrothion [\u003ca href=\"#_ENREF_18\"\u003e18\u003c/a\u003e], indicating that other than \u003cem\u003ekdr\u003c/em\u003e mutations, or more likely, enzymatic mechanisms are involved in conferring resistance in mosquitoes. In Central Java, Indonesia, although the G119S mutation was absent, a degree of resistance to malathion was observed in \u003cem\u003eAe. aegypti\u003c/em\u003e [\u003ca href=\"#_ENREF_1\"\u003e1\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003eReports on target-site mutations on \u003cem\u003eVGSC\u003c/em\u003e and \u003cem\u003eace-1\u003c/em\u003e genes of \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e are rare compared of that on \u003cem\u003eAe. aegypti\u003c/em\u003e. However, the insecticide resistance level of the \u003cem\u003eCx. quinquefasciatus \u003c/em\u003ein Jeddah may even worse as inferred from the fixation of mutation allele both on \u003cem\u003eVGSC\u003c/em\u003e and \u003cem\u003eace-1\u003c/em\u003e genes. In the studied mosquito samples in Jeddah, the \u003cem\u003ekdr\u003c/em\u003e homozygous genotype TTT/TTT occupied most (90.48%) of the individuals. While the heterozygous genotype GGC/AGC on \u003cem\u003eace-1\u003c/em\u003e gene was detected in all samples of \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e. This is probably related to constant exposure due the extensive use of insecticides for routine spraying campaigns of public health, or for domestic use against mosquito vectors and other insect pests in Jeddah region. Similarity, the homozygous susceptible 1014 genotype was absent in samples collected in 2011 from Zanzibar, East Africa [\u003ca href=\"#_ENREF_74\"\u003e74\u003c/a\u003e]. In India, most of the studied mosquito samples harbored the heterozygous genotype TTT/TTA, with the frequency of 75% in \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e samples [\u003ca href=\"#_ENREF_43\"\u003e43\u003c/a\u003e].\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eLimitations of this study\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eOne major limitation of our study is the small sample sizes of mosquitoes used for detection on target site mutations of insecticide resistance genes. Whereas, the primary survey reveals the widespread of\u003cem\u003e kdr \u003c/em\u003ein predominant mosquitoes in Jeddah. Further, bioassay tests on both larvae and adult mosquitoes are required to evaluate the status of insecticide resistance in practice. And the underlying metabolic mechanism responsible for insecticide resistance is worth exploring.\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003eThe current study involved a wider geographic region of \u003cem\u003eAe. aegypti\u003c/em\u003e vector populations in Jeddah. The results showed that six genotypes of the three codon combinations, 989, 1016, and 1534 were spreading in Jeddah\u0026rsquo;s \u003cem\u003eAe. aegypti\u003c/em\u003e population, with the PGF/PGC as the dominate one. Two types of introns between exon 20 and 21 on \u003cem\u003eVGSC\u003c/em\u003e for the first time are identified in Saudi Arabia. Linkage associations have been shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. High prevalence of \u003cem\u003ekdr\u003c/em\u003e mutations implies that the local \u003cem\u003eAe. aegypti\u003c/em\u003e mosquitoes are pyrethroid-resistant, and the level of resistance may increase with the extensive and intensive use of pyrethroid, as the active ingredients in household insecticide and spatial repellents. To the best of our knowledge, this is the first report of resistant-associated mutations in field caught \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e in Saudi Arabia. The fixation of L1014F allele on \u003cem\u003eVGSC\u003c/em\u003e and G119S on \u003cem\u003eace-1\u003c/em\u003e gene was detected in local \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e populations, with the frequency of 95.24% and 100%, demonstrating that traditional insecticides are likely to be losing efficacy through fogging activities or IRS. In addition, analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (P = 0.000) allele type, and may reflect the history of insecticide treatment in different continents.\u003c/p\u003e\n\u003cp\u003eThe primary analyses showed the widespread of \u003cem\u003ekdr\u003c/em\u003e mutation both in \u003cem\u003eAe. aegypti\u003c/em\u003e and \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e. The situation may even worse with increasing urbanization, climate changes and sustained Aedes-Culex endemicity estimated at 31\u0026ndash;56% prevalence rate, thus threating the efficiency of vector control against mosquito-borne diseases. It raises the attention that wide-range of surveillance the polymorphism and distribution of insecticide target site mutations in mosquitoes in this region. The mapping of the nation-wide distribution and polymorphism of target site mutation against insecticide resistance will provide valuable consensus for forecasting the status of insecticide resistance in mosquito populations of Jeddah and other regions of Saudi Arabia. Additionally, regular monitoring and documenting the dynamics of target mutation gene and their associations with the insecticide resistance phenotype is important in the surveillance system of preventing mosquito-borne diseases through long-term and sustainable vector control strategies.\u003c/p\u003e\n\u003cp\u003eMeanwhile, the above findings suggest that alternative insecticides in areas, and proper use of insect growth regulator or biocontrol approaches for integrated vector control programs should be considered in Jeddah and neighborhoods to mitigate and slow down the spread and impact of insecticide resistance development in disease vector populations. Albeit, substantial Saudi government and Jeddah municipality commit and invest in innovative operational research, research and development on more early-detection of insecticide resistance and effective control measures and tools for discovery and utilization, including vaccines for local and pilgrims\u0026rsquo; immunization programs implementation. With the establishment of the Public Health Pest Laboratory of Jeddah Governorate, sustainable funding is imperative to provide evidence for coherent and coordinated temporal and spatial epidemiological IVM and pesticide choices implementation amongst all stakeholders. Furthermore, strengthening laboratory and epidemiologic capacity building programs for health workers, health professional, and community engagement including and private-public sectors partnerships on mosquito vector, dengue and emerging threat and epidemics sustained control towards elimination.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003e\u003cem\u003eace-1\u003c/em\u003e: acetylcholinesterase 1; DENV: dengue virus; \u003cem\u003ekdr\u003c/em\u003e: knockdown resistance; K2P: Kimura\u0026rsquo;s 2-parameter; ML: maximum likelihood; IRS: indoor residual spraying; IVM: integrated vector management; VGSC: voltage-gated sodium channel\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNo specific permits were required for this study. The study did not involve endangered or protected species.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll data generated or analyzed during this study are included in this published article. The newly generated sequences were submitted to the GenBank database under the accession numbers MN997310\u0026ndash;MN997399.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe Special Foundation of Basic Science and Technology Resources Survey of Ministry of Science and Technology of China (grant no. 2017FY101200), the Project of Basic Platform of National Science and Technology Resources of the Ministry of Sciences and Technology of China (grant no. TDRC-2019-194-30).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor\u0026rsquo;s contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe paper was conceived \u003c/strong\u003eTambo Ernest, Yuan Fang, Emad I.M. Khater, Yi Zhang.\u003cstrong\u003e The investigation was conducted\u003c/strong\u003e by Yuan Fang, Emad I.M. Khater, Tambo Earnest,\u003cstrong\u003e Data \u003c/strong\u003e\u003cstrong\u003ecuration was performed by \u003c/strong\u003eTambo Ernest, Emad I.M. Khater for mosquito collection and providing reagents and materials, Yuan Fang for conducting molecular work and phylogenetic and statistical analyses, and Jing-Bo Xue for drawing map of mosquito collection sites. \u003cstrong\u003eFunding acquisition:\u003c/strong\u003e Yi Zhang, Tambo Ernest, Emad I.M. Khater and Xiao-Nong Zhou. Yuan Fang, Tambo Ernest and Yi Zhang \u003cstrong\u003ewrote the first draft \u003c/strong\u003eand Yuan Fang, Tambo Ernest, Yi Zhang, Jing-Bo Xue, Emad I.M. Khater\u003cstrong\u003e reviewed and edited\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eSayono S, Hidayati APN, Fahri S, Sumanto D, Dharmana E, Hadisaputro S, et al. Distribution of voltage-gated sodium channel (\u003cem\u003eNav\u003c/em\u003e) alleles among the \u003cem\u003eAedes aegypti\u003c/em\u003e populations in Central Java Province and its association with resistance to pyrethroid insecticides. PLoS One. 2016;11:e0150577.\u003c/li\u003e\n\u003cli\u003eLiu H, Xie L, Cheng P, Xu J, Huang X, Wang H, et al. Trends in insecticide resistance in \u003cem\u003eCulex pipiens pallens\u003c/em\u003e over 20\u0026nbsp;years in Shandong, China. Parasite Vector. 2019;12:167.\u003c/li\u003e\n\u003cli\u003eMoyes CL, Vontas J, Martins AJ, Ng LC, Koou SY, Dusfour I, et al. 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Insecticide resistance in \u003cem\u003eAnopheles arabiensis\u003c/em\u003e (Diptera: Culicidae) from villages in central, northern and south west Ethiopia and detection of \u003cem\u003ekdr\u003c/em\u003e mutation. Parasit Vectors. 2010;3:40.\u003c/li\u003e\n\u003cli\u003eWeaver SC, Charlier C, Vasilakis N, Lecuit M. Zika, chikungunya, and other emerging vector-borne viral diseases. Annu Rev Med. 2018;69:395\u0026ndash;408.\u003c/li\u003e\n\u003cli\u003eWHO. Test procedures for insecticide resistance monitoring in malaria vector mosquitoes. Geneva: WHO2013.\u003c/li\u003e\n\u003cli\u003eJones CM, Machin C, Mohammed K, Majambere S, Ali AS, Khatib BO, et al. Insecticide resistance in \u003cem\u003eCulex quinquefasciatus\u003c/em\u003e from Zanzibar: implications for vector control programmes. Parasit Vectors. 2012;5:78.\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eTable 1. Frequency (percentage) of triple-locus \u003cem\u003ekdr\u003c/em\u003e genotypes, shown as amino acid codon number 989, 1016 and 1534 in the voltage-gated sodium channel gene (\u003cem\u003eVSGC\u003c/em\u003e) of \u003cem\u003eAedes aegypti\u003c/em\u003e collected from Jeddah, Saudi Arabia.\u003c/span\u003e\u003c/p\u003e\n\u003cdiv align=\"center\" style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:等线;\"\u003e\n \u003ctable style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.4pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:right;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eGenotype\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.9pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eSVF/PGF\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.9pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eSVF/SVC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.1pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eSVC/SVC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.1pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePGF/PGF\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 60.35pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePGF/PGC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.55pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePGC/PGC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.4pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eNo.\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:left;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:left;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:left;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e5\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:left;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 60.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:left;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e9\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.55pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.4pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePercentage (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.9pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e5.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.9pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e5.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.1pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e25.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 59.1pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e15.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 60.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e45.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 61.55pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e5.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eTable 2. Frequency (percentage) of point mutations (S989P, V1016G, F1534C) and intron types identified in the voltage-gated sodium channel gene (\u003cem\u003eVSGC\u003c/em\u003e) of \u003cem\u003eAedes aegypti\u003c/em\u003e collected from Jeddah, Saudi Arabia.\u003c/span\u003e\u003c/p\u003e\n\u003cdiv align=\"center\" style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:等线;\"\u003e\n \u003ctable style=\"width:559.7pt;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:right;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eCodon\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"4\" style=\"width: 108.95pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e989\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"4\" style=\"width: 108.95pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1016\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"4\" style=\"width: 115.15pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1534\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 138.2pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eIntron type between exons 20 and 21\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eAllele type\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.65pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eS\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 53.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eP\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eV\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 53.5pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eG\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 59.5pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eF\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.65pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eType A\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 80.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eType B\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eNo.\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.65pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e13\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 53.3pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e27\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e13\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 53.5pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e27\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 59.5pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e18\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.65pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e22\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e28\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 80.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e12\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePercentage (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.65pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e32.50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 53.3pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e67.50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.45pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e32.50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 53.5pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e67.50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 59.5pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e45.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 55.65pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e55.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.1pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e70.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 80.1pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e30.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eGenotype\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eSS\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 32.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eSP\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePP\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eVV\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 32.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eVG\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eGG\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eFF\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 38.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eFC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eCC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eType A\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 80.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eType B\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eNo.\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e6\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 32.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e13\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e6\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 32.25pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e13\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 38.45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e10\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e6\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e14\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 80.1pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e6\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 88.45pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003ePercentage (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e30.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 32.25pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e5.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e65.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e30.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 32.25pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e5.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e65.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e20.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 38.45pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e50.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 38.35pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e30.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 58.1pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e70.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 80.1pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e30.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd style=\"border:none;\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp style=\"margin:0in;margin-bottom:6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eTable 3. Chi\u003csup\u003e\u0026nbsp;\u003c/sup\u003esquare analysis of the linkage between point mutations and type A and B introns located between exons 20 and 21 on voltage-gated sodium channel gene (\u003cem\u003eVGSC\u003c/em\u003e) reported in \u003cem\u003eAedes aegypti\u003c/em\u003e from Jeddah, Saudi Arabia and homologous sequences retrieved from the GenBank from other geographic regions.\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003ctable style=\"border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 89.85pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width: 91.9pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e\u0026chi;\u003csup\u003e2\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eDf\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border-top: 1pt solid windowtext;border-left: none;border-bottom: 1pt solid windowtext;border-right: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cem\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eP\u003c/span\u003e\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"5\" style=\"width: 89.85pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1016\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 45.95pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e982\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 45.95pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eJeddah\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e22.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width: 45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e989\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 46.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eJeddah\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e44.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"2\" style=\"width: 46.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eGlobal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e49.910\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width: 45pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1534\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 46.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eJeddah\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e15.402\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.004\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"2\" style=\"width: 46.9pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eGlobal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e16.297\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.003\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"4\" style=\"width: 89.85pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eIntron type between exons 20 and 21\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd rowspan=\"2\" style=\"width: 45pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1016\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width: 46.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eJeddah\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e22.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"2\" style=\"width: 46.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003eGlobal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e34.333\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.75pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width: 45pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e1534\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" 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\u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 89.9pt;border-top: none;border-right: none;border-left: none;border-image: initial;border-bottom: 1pt solid windowtext;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style=\"margin:0in;margin-bottom: 6.0pt;text-align:justify;font-size:14px;font-family:等线;margin-top:6.0pt;margin-right:0in;margin-left:0in;line-height:150%;\"\u003e\u003cspan style='font-size:15px;line-height:150%;font-family:\"Calibri\",\"sans-serif\";'\u003e0.000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n 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channel gene","lastPublishedDoi":"10.21203/rs.3.rs-35782/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-35782/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground:\u003c/strong\u003e Gene mutations on target sites can be a valuable reference to the status of insecticide resistance. Jeddah, a global commercial and major port-of-entry city, is bearing the most (\u0026gt;70%) dengue disease burden and the population of the dengue vector \u003cem\u003eAedes aegypti\u003c/em\u003e (Linnaeus, 1762) in Saudi Arabia. \u003cem\u003eCulex quinquefasciatus\u003c/em\u003e Say, 1823 is a second example as one of the major arboviral vectors in the region. However, the status of insecticide resistance and targeted site mutations on the responsible genes are not fully characterized.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods: \u003c/strong\u003eWe randomly sampled both mosquito species, \u003cem\u003eAe. aegypti\u003c/em\u003e (25 individuals) and \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e (24 individuals) across Jeddah by daily mosquito surveillance in 2016 to detect the resistance-associated target site mutations on the voltage gated sodium channel (\u003cem\u003eVGSC\u003c/em\u003e) and acetylcholinesterase 1 (\u003cem\u003eace-1\u003c/em\u003e) genes by PCR amplification and direct sequencing. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults: \u003c/strong\u003eOur findings showed that \u003cem\u003eAe. aegypti\u003c/em\u003e resistance-associated \u003cem\u003eVGSC \u003c/em\u003egene mutations revealed polymorphic mutations on the 989, 1016, and 1534 sites. Additionally, we documented two types of introns between exons 20 and 21, however, the I1011M point mutation was undetected. Linkage disequilibrium associations were shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. Furthermore, no mutation on \u003cem\u003eace-1\u003c/em\u003e was identified in \u003cem\u003eAe. aegypti\u003c/em\u003e. In \u003cem\u003eCx. quinquefasciatus\u003c/em\u003e, homozygous L1014F/L1014F (95.23%) on the \u003cem\u003eVGSC\u003c/em\u003e and heterozygous G119/G119S (100%) on \u003cem\u003eace-1\u003c/em\u003e were widely distributed in the samples studied. Analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (P = 0.000) allele type, and may reflect the history of insecticide treatment in different continents.\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusions: \u003c/strong\u003eTo the best of our knowledge, this is the first record of the intron types between exons 20 and 21 on \u003cem\u003eVGSC\u003c/em\u003e of \u003cem\u003eAe. aegypti\u003c/em\u003e populations. It can serve as a reference marker to evaluate the local insecticide resistance status. In addition, the first report of insecticide resistance gene mutation being present in field caught \u003cem\u003eCx. quinquefasciatus \u003c/em\u003ein Saudi Arabia. High prevalence of insecticide resistance gene mutations in local primary mosquito vector species alert the urgent need to carry out a comprehensive insecticide resistance surveillance.\u0026nbsp;\u003c/p\u003e","manuscriptTitle":"Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2020-10-05 20:36:27","doi":"10.21203/rs.3.rs-35782/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2020-06-16 16:02:33","doi":"10.21203/rs.3.rs-35782/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"39ea1176-9882-4ef1-93da-37d8a20aee24","owner":[],"postedDate":"October 5th, 2020","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":697299,"name":"Parasitology"}],"tags":[],"updatedAt":"2020-10-22T23:12:28+00:00","versionOfRecord":[],"versionCreatedAt":"2020-10-05 20:36:27","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-35782","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-35782","identity":"rs-35782","version":["v2"]},"buildId":"_2-kVJe1T_tPrBINL-cwx","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}