Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles

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This study aimed to the effects of various newly formulated products on the amino acid composition of the diet and whole-body carcass, and to analyse the growth coefficient, body indices, and gut micromorphology. Methods: One hundred gram of palm sap sugar was cooked in 1.1 litre of fresh water for fifteen minutes, to create 1 litre of 11% palm sap sugar solution (after some of it had been boiled off). Two litres of coconut water were then mixed with the litre of palm sugar solution. One litre of this product was added in turn to 2 g of Aspergillus niger (CP2), 2 g of Rhizopus oligosporus (CP3), and 2 g of Saccharomyces cerevisiae (CP4), while freshwater was used as a control (labeled CP1). Aquafeed was added to CP1, CP2, CP3, and CP4, to make diets labeled KP1, KP2, KP3, and KP4. The dosage was 150 ml/kg of feed. Juvenile giant gourami (initial weight 50±0.25 g and length 13.2±0.07 cm) were reared in triplicate net frames (2×1×1 m; water volume 1.5 m 3) in a freshwater concrete pond with a stocking density of 30 juveniles/net. Results: The results supported our hypothesis that different product formulations have a significant effect (P < 0.05) on aquafeed nutrition and the whole-body carcass, growth coefficient, feed utilization, body indices, and gut micromorphology of giant gourami juveniles. The thermal growth coefficient strongly correlated with the daily growth coefficient (r 2 = 92%), condition factor (r2 = 77%), protein efficiency ratio (r2 = 75%), while a moderate relationship with the feed intake ( r2 = 69%). Conclusions: Diet KP3 contains higher total amino acids in diets and carcasses and leads to feed efficiency and better growth for giant gourami." } { "@context": "http://schema.org", "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": "1", "item": { "@id": "https://f1000research.com/", "name": "Home" } }, { "@type": "ListItem", "position": "2", "item": { "@id": "https://f1000research.com/browse/articles", "name": "Browse" } }, { "@type": "ListItem", "position": "3", "item": { "@id": "https://f1000research.com/articles/12-140", "name": "Effect of feed enriched by products formulated from coconut water,..." } } ] } Home Browse Effect of feed enriched by products formulated from coconut water,... ALL Metrics - Views Downloads Get PDF Get XML Cite How to cite this article Undefined A, Syandri H, Aryani N and Mardiah A. Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.12688/f1000research.124706.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. Close Copy Citation Details Export Export Citation Sciwheel EndNote Ref. Manager Bibtex ProCite Sente EXPORT Select a format first Track Share ▬ ✚ Research Article Revised Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] Azrita Undefined https://orcid.org/0000-0003-2407-5587 1 , Hafrijal Syandri https://orcid.org/0000-0002-3684-8842 1 , Netti Aryani https://orcid.org/0000-0003-1960-4288 2 , Ainul Mardiah 3 Azrita Undefined https://orcid.org/0000-0003-2407-5587 1 , Hafrijal Syandri https://orcid.org/0000-0002-3684-8842 1 , Netti Aryani https://orcid.org/0000-0003-1960-4288 2 , Ainul Mardiah 3 PUBLISHED 11 Sep 2023 Author details Author details 1 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, West Sumatera, 25113, Indonesia 2 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Riau, Pekanbaru, 28293, Indonesia 3 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Nahdlatul Ulama, Padang, West Sumatera, 25118, Indonesia Azrita Undefined Roles: Conceptualization, Data Curation, Formal Analysis, Methodology, Validation, Writing – Original Draft Preparation Hafrijal Syandri Roles: Data Curation, Funding Acquisition, Investigation, Methodology, Validation, Writing – Review & Editing Netti Aryani Roles: Data Curation, Formal Analysis, Methodology, Writing – Original Draft Preparation Ainul Mardiah Roles: Data Curation, Software, Writing – Original Draft Preparation OPEN PEER REVIEW DETAILS REVIEWER STATUS This article is included in the Agriculture, Food and Nutrition gateway. Abstract Background: Giant gourami, Osphronemus goramy is the most important freshwater fish species produced by aquaculture in Indonesia. This study aimed to the effects of various newly formulated products on the amino acid composition of the diet and whole-body carcass, and to analyse the growth coefficient, body indices, and gut micromorphology. Methods: One hundred gram of palm sap sugar was cooked in 1.1 litre of fresh water for fifteen minutes, to create 1 litre of 11% palm sap sugar solution (after some of it had been boiled off). Two litres of coconut water were then mixed with the litre of palm sugar solution. One litre of this product was added in turn to 2 g of Aspergillus niger (CP2), 2 g of Rhizopus oligosporus (CP3), and 2 g of Saccharomyces cerevisiae (CP4), while freshwater was used as a control (labeled CP1). Aquafeed was added to CP1, CP2, CP3, and CP4, to make diets labeled KP1, KP2, KP3, and KP4. The dosage was 150 ml/kg of feed. Juvenile giant gourami (initial weight 50±0.25 g and length 13.2±0.07 cm) were reared in triplicate net frames (2×1×1 m; water volume 1.5 m 3 ) in a freshwater concrete pond with a stocking density of 30 juveniles/net. Results: The results supported our hypothesis that different product formulations have a significant effect (P < 0.05) on aquafeed nutrition and the whole-body carcass, growth coefficient, feed utilization, body indices, and gut micromorphology of giant gourami juveniles. The thermal growth coefficient strongly correlated with the daily growth coefficient (r 2 = 92%), condition factor (r 2 = 77%), protein efficiency ratio (r 2 = 75%), while a moderate relationship with the feed intake ( r 2 = 69%). Conclusions: Diet KP3 contains higher total amino acids in diets and carcasses and leads to feed efficiency and better growth for giant gourami. READ ALL READ LESS Keywords Giant gourami, amino acid profile, growth performance, palm sap sugar, coconut water, gut micromorphology Corresponding Author(s) Azrita Undefined ( [email protected] ) Close Corresponding author: Azrita Undefined Competing interests: No competing interests were disclosed. Grant information: We are grateful to the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia for funding this research under grant number: 076/E5/PG.02.00. PT/2022 The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Copyright: © 2023 Undefined A et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. How to cite: Undefined A, Syandri H, Aryani N and Mardiah A. Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.12688/f1000research.124706.2 ) First published: 07 Feb 2023, 12 :140 ( https://doi.org/10.12688/f1000research.124706.1 ) Latest published: 11 Sep 2023, 12 :140 ( https://doi.org/10.12688/f1000research.124706.2 ) Revised Amendments from Version 1 We have consistently used “giant gourami” and "whole-body carcasses" throughout this article. Furthermore, several scientific names have been italicized in the abstract and introduction; Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae . Additionally, we added the gut micromorphology of giant gourami juveniles for each experiment (Figure 4) and regression values for the thermal growth coefficient, condition factor, protein efficiency ratio, and feed intake in the abstract and conclusion. We have consistently used “giant gourami” and "whole-body carcasses" throughout this article. Furthermore, several scientific names have been italicized in the abstract and introduction; Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae . Additionally, we added the gut micromorphology of giant gourami juveniles for each experiment (Figure 4) and regression values for the thermal growth coefficient, condition factor, protein efficiency ratio, and feed intake in the abstract and conclusion. See the authors' detailed response to the review by Fatimah Hashim and Nor Omaima Harun See the authors' detailed response to the review by Qadar Hasani READ REVIEWER RESPONSES Introduction In this decade, the production of capture fisheries has decreased; meanwhile, the demand for fish products for human consumption is increasing. Therefore, according to the Food and Agriculture Organisation, 60% of fisheries production in the future will come from aquaculture activities and this figure will continue to rise. 1 The utilization of a variety of fish for aquaculture has now increased the need for commercial feed. 2 – 5 At the same time, for aquaculture operations, the cost of aquafeed is still a significant challenge. 2 , 6 – 8 On the other hand, commercial feed produced by factories still does not contain complete nutrition for fish growth, while being acknowledged for its positive effects on food safety. 9 – 11 In this context, enriching fish feed with cost-effective natural ingredient resources is key to increasing feed nutrient quality and feed efficiency in commercial fish farming and ensuring the sustainability of aquaculture operations. 2 , 12 , 13 The target is fish feed that is wealthy in many important nutrients, including protein, fat, vitamins, and minerals that cultured fish can utilize to increase their growth rate and survival and that is beneficial for human health. 4 , 14 – 16 Therefore, novel approaches have been developed by scientists to improve the nutrition of fish feeds, such as feed supplemented with EPA and DHA, 17 iodine and selenium, 10 methionine, 18 fish oil, 11 , 19 and soybean oil. 20 In addition, supplementing probiotics into the diet 21 and supplemental glycine, prebiotics, and nucleotides in a soybean meal-based diet have been studied. 22 The progress of aquaculture biotechnology has stimulated the interest of scientists in improving aquatic animal production, for example, to increase giant gourami production. One of the experimental techniques is to increase feed nutrition used for this purpose, such as, the use of fish meal and Azolla ( Azolla pinnata ) flour as a feed ingredient for giant gourami, 23 and the utilization of new products formulated from water coconut, palm sap sugar, and fungus for the enrichment of commercial feed. 9 Additional research has involved a diet supplemented using glutamine, 24 feed supplemented with a growth hormone, 25 and substitute fish meal incorporating chicken feather. 26 Whether using coconut water and palm sap sugar fermented with mushrooms ( Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae ) affects the amino acid composition of the diet, body carcass, growth coefficient, and body indices is still not understood. Coconut water has extraordinary nutritional value and contains supplements for health like minerals, amino acids, fatty acids, vitamins, enzymes, organic acids, and several phenolic compositions. 27 – 30 Palm sap sugar also has health benefits due to its essential nutrient content, such as a low glycaemic index, and it contains antioxidants, vitamins, and minerals. 31 – 34 Meanwhile, mushrooms have been widely used in fermentation due to their ability to degrade antigenic proteins in fish feed ingredients. 7 , 35 , 36 Additionally, coconut water is a functional food that can protect the lens from diabetic cataract development in rats. 37 Coconut water is also a treatment for burning pain during urination, dysuria, gastritis, increasing semen, and indigestion. 38 On the other hand, Azrita et al. 9 have reported using new formulations of products containing coconut water and palm sap sugar that are fermented with various mushrooms involving a dosage of 300 ml/kg feed. Their newly formulated products can increase fatty acid levels in the diet and whole-body carcasses. Besides that, they also improve giant gourami's growth performance and feed efficiency. However, the effect of these new formulation products at a dosage of 150 ml/kg feed on the diet amino acid composition, and body meat's amino acid composition has not yet been analyzed. In line with that, the relationships between thermal growth coefficient and condition factor, daily growth coefficient, and feed utilization coefficient, including body indices parameters, as well as the gut micromorphology of giant gourami, have not yet been analyzed. We hypothesized that commercial aquafeed combined with different newly formulated products at the dosage of 150 ml/kg feed could improve the amino acids compositions of the aquafeed and whole-body carcass, body indices, and gut micromorphology. Hence, this investigation's first purpose was to analyze the effect of various newly formulated products on the diet's proximate compositions, amino acid composition, and whole-body carcass. The second aim was to analyze the impact of newly formulated products on condition factor, the growth coefficient and relation to thermal growth coefficient, body indices, and gut micromorphology in giant gourami juveniles. Methods Ethical approval The Research and Community Service Ethics Committee at Universitas Bung Hatta, West Sumatera, Indonesia approved this research (89/LPPM/Hatta/III-2022) which followed the ARRIVE guidelines. The Ministry of Education, Culture, Research and Technology of the Republic of Indonesia funded the research under grant No. 076/E5/PG.02.00 PT/2022 on March 16, 2022. Approval was given by the ethics committee to collect and rear juvenile of giant gourami in the aquaculture laboratory, Faculty of Fisheries and Marine Science at Universitas Bung Hatta. All efforts were made to relieve the suffering of experimental animals. Therefore, the animal did not suffer for this study, and they were still in good condition when returned to the pond after research was completed. Where some fish were euthanized, this was carried out by piercing part of the fish’s brain. Giant gourami are not classified as a protected animal according to Indonesian legislation. Preparation of formulated product We prepared 100 g of palm sap sugar by traditional production and cooked it in 1.1 litre of fresh water for fifteen minutes at 60 0 C, and only got 1 litre of palm sap sugar solution due to some of it being boiled off. Then, this was cooled in an open space for twenty minutes. Furthermore, we also prepared 2.0 litres of mature coconut water ( Cocos nucifera L . ) and mixed it with the 1.0 litre of palm sap sugar solution. The product was stored for ten minutes in a cool air-conditioned room. A total of 3.0 litres of the formulated product was divided into three parts of 1.0 litre each. We added 2 g of Aspergillus niger (labeled as CP2 product) to the first part of the formulated product solution, 2 g of Rhizopus oligosporus (labeled as CP3 product) to the second part, and 2 g of Saccharomyces cerevisiae (labeled as CP4 product) to the third portion. The CP2, CP3, and CP4 products were fermented for 48 hrs in a jerry can (2.0 litres) using an Aerasi PUJIMAC, MAC-40 K 40 L/min. The products of CP2, CP3, and CP4 were used to enrich the nutrition of commercial aquafeed (781-2, PT. Japfa Comfeed Indonesia, Tbk) and labeled as the KP2, KP3, and KP4 diets. The aquafeed was supplemented with freshwater (labeled as the KP1 diet; placebo). Preparation of experimental diets Giant gourami juveniles were adapted for one month to standard feed, namely floating commercial aquafeed 781-2 (pellet size 2 mm), which contained 10.66% water content, 30.10% crude protein, 4.09% crude fat, 45.35% total carbohydrates, 2.5% ash, and 9.18% crude fibre. The minerals in the commercial feed were 280.08 mg/100 g Na, 1415.02 mg/100 g Ca, 1358.07 mg/100 g K, 1200.31 mg/100 g P, 292.03 mg/100 g Mg, 18.14 mg/100 g Fe, and 13.83 mg/100 g Zn. The aquafeed was added to freshwater to create the KP1 diet as observed, and the formulated CP2, CP3, and CP4 products were added to the aquafeed at a dosage of 150 ml/kg of feed to create the enriched fish diets. The formulated product added to the aquafeed was mixed manually with it for three minutes to obtain maximum homogenization and then the blend was dried in the open air for thirty minutes. Thereafter, it was given to the trial animal. Experimental procedures and sampling In the present study, we measured fish weight using AD-600i scales with 0.001 g accuracy (ACIS model number AD-600i, China). At the same time, a meter ruler with 1 mm accuracy was used to estimate the body length. A total of 360 sago strain juveniles of giant gourami were counted; the initial mean weight was 50 ± 0.25 g, and the initial length was 13.2±0.07 cm. For rearing juveniles, twelve nets framed with 2 m 3 (2 × 1 × 1 m) PVC pipe (water volume of 1.5 m 3 ) were placed inside two freshwater concrete ponds with a size of 18 m 3 (6 × 2 × 1.5 m). This experiment consisted of four treatments and three replications, and each frame net was stocked with 30 juveniles. The giant gourami were fed the KP1, KP2, KP3, and KP4 diets three times a day (08:00, 12:00, and 17:00 hrs) during the 90-day feeding trial. Juveniles of giant gourami were fed at a 3% body weight rate per day based on the percentage of stored biomass. Fish samples were collected every 30 days for body weight and length measurements. Ten fish per net frame were collected and anesthetized orally using clove oil. Then, their lengths and weights were measured. Prior to sampling, the fish fasted for 24 hrs to empty their intestinal contents. Proximate and amino acid composition The diet samples and proximate carcass composition were analyzed using standard AOAC methods. 39 The matter was dried to a constant weight at 105°C. We used the standard Kjeldahl method to analyse crude protein (N × 6.25). We used the Soxhlet method with ether extraction to analyse crude lipids; the ash was incinerated at 550°C for 16 hrs, whereas gross energy was measured in a bomb calorimeter. The amino acid composition was determined by using a high-performance liquid chromatography (HPLC) system consisting of a water 1525 binary HPLC pump, 717 autosamplers (water ® ), and water 2475 multi λ fluorescence detector optics (wavelengths: 250 nm for excitation and 395 nm for emission). It was hydrolysed in triplicate with 6 N hydrochloric acid for 24 hrs at 11°C. 40 Nutrient utilization and body indices The growth coefficients in the fish experiments were measured by using the thermal growth coefficient (TGC), daily growth coefficient (DGC), total feed intake (FI), and protein efficiency ratio (PER) of giant gourami, assessed using the following formulae: TGC = final weight g ⅓ – initial weight g ⅓ / [ mean water temperature ° C × duration of rearing period day ] × 1000 DGC = Wf ⅓ – Wi ⅓ / duration of rearing period day × 100 FI as feed FI as feed in g / fish / day = Total feed fed / n × t PER = wet weight gain / total protein intake Three fish from each net frame were sacrificed and dissected immediately to determine the Condition factor (CF), Viscerosomatic index (GSI%), Hepatosomatic index (HSI%), Visceral fat-somatic indexes (VFSI%), and Bilesomatic index (BSI) as given below: CF = 100 × weight of the juvenile g / Length of juvenile cm 3 GSI = 100 × viscera weight g / whole body weight g HSI = 100 × liver weight g / whole body weight g VFSI = 100 × visceral fat weight g / whole body weight g BSI = 100 × Bile weight g / weight of liver g Histological examination of the gut For histological analyses, each gut specimen of the animal was cut into the foregut, midgut, and hindgut. Moreover, the cells were cleaned in saline solution and fixed in Bouin's fixative solution for 24 hrs. After sequential dehydration steps in alcohol, the gut samples were embedded in paraffin. The implanted tissue blocks were sectioned at 5 μm, and sections were consistently stained with Haematoxylin-eosin and observed under a light microscope (Olympus IX71) equipped with Image-Pro Plus 7.0 software, Japan. The digitalized analysis measures the micrometer length of various enteric structures of gut images. We determined the average fold height (hF), fold width (wF), and enterocyte height (hMV) of the gut per slice (5 fields per individual sample) according to procedures described by Li et al. 18 The specific measurement method of gut samples is shown in Figure 1 . Figure 1. Transversal section photomicrographs of giant gourami juvenile foregut. (A) Fold height and fold width were analyzed in a lower magnification of objective lens of microscope (magnification × 100), (B) Enterocytes height and microvilli height were analyzed using a higher magnification of an objective lens microscope (magnification × 200). hF = fold height, wF = fold width, hE = enterocyte height, hMV = microvillus height (hematoxylin and eosin). Pond water quality The water quality values of the freshwater concrete ponds that were used to rear the giant gourami juveniles were recorded weekly. The water samples were collected at 10:00 am at a depth of 20 cm from each concrete pond to determine the water temperature, dissolved oxygen, and pH value. In addition, we also measured the total alkalinity, hardness, and nitrates of the water in the pond experiments. A thermometer (Celsius scale) was used to measure water temperature. To measure water dissolved oxygen (O 2 ; mg L -1 ), we used an oxygen meter (YSI Model 52, Yellow Instrument Co, Yellow Spring, OH USA). A digital pH meter (Mini 0–14 pH IQ, Scientific Cemo Science, Thailand) was used to determine the pH values of water in the experiments. The level of nitrate-nitrogen (NO 3 -N; mg L -1 ), alkalinity (mg L -1 ), and hardness (mg L -1 ) were measured according to standard procedures. 41 Calculations and statistical method The data from this study were reported in the form of the mean ± standard deviation for each treatment. Data were analysed using the SPSS 16.0 software package (SPSS; Chicago, IL). Normality was tested using the Kolmogorov–Smirnov statistic. Homogeneity was checked using absolute residuals according to Levine's test. One-way ANOVA was used to determine the treatment effect, followed by a post-hoc Duncan's multiple range test. 42 To create the figures, Microsoft Office Professional Plus 2019 was used. Results Proximate and amino acid profiles of the diets Commercial feed supplemented with different formulated products with the dosage of 150 ml/kg of feed significantly affects the proximate composition of diets. One-way ANOVA results showed a marginal interaction among treatments in the case of protein content (F (3,8) = 1.522, P = 0.282), fat (F (3,8) = 5.663, P = 0.022), carbohydrates (F (3,8) = 1.862, P = 0.214), crude fibre (F (3,8) = 1.445, P = 0.300), and ash (F (3,8) = 0.272, P = 0.844), and the total energy content (F (3,8) =1.112, P = 0.400) differed considerably ( P < 0.05) among the four diets ( Table 1 ). Duncan's Post-hoc test revealed that the protein content (21.6967 ± 0.17%) was significantly higher ( P < 0.05) in the KP3 diet than in the other treatments, while the carbohydrate (31.19 ± 0.38%), crude fibre (2.82 ± 0.06%), and ash (6.57 ± 0.04%) contents were significantly higher ( P < 0.05) in the KP3 diet than in the other diets. Conversely, the total energy content was 240.88 ± 0.74 (kg calories/100 g), which was significantly higher ( P < 0.05) in the KP3 diets than in the KP1, KP2, and KP4 diets ( Table 1 ). Table 1. The experimental diets' proximate and amino acid composition (% dry matter). Mean ± SD * . KP1 KP2 KP3 KP4 %, dry weight basis Proximate composition Dry matter 38.42 ± 0.25 a 38.27 ± 0.01 a 37.59 ± 0.16 a 38.41± 0.10 a Crude protein 19.68 ± 0.41 a 20.27 ± 0.13 b 21.70 ± 0.18 c 20.44 ± 0.10 d Crude lipid 3.41 ± 0.02 a 3.67 ± 0.13 b 3.50 ± 0.02 ac 3.48 ± 0.04 ad Carbohydrate 26.37 ± 0.17 a 29.50 ± 0.54 b 31.19 ± 0.38 c 30.57 ± 0.06 d Crude fibre 2.23 ± 0.05 a 2.36 ± 0.01 b 2.82 ± 0.06 c 2.45 ± 0.06 d Ash 2.75 ± 0.03 a 6.66 ± 0.05 b 6.57 ± 0.04 c 6.67 ± 0.06 d Energy total (kg calorie/100 g) 240.87 ± 0.38 a 234.41 ± 0.30 b 240.88 ± 0.74 ac 237.11 ± 0.43 d Amino acid composition EAA Leucine 1.36 ± 0.01 a 1.42 ± 0.01 b 1.46 ± 0.01 c 1.36 ±0.01 d Isoleucine 0.76 ± 0.01 a 0.79 ± 0.01 b 0.81 ± 0.01 c 0.76 ± 0.01 d Lysine 0.95 ± 0.01 a 1.10 ± 0.01 b 0.98 ± 0.01 c 1.20 ± 0.01 d Valine 0.86 ± 0.01 a 0.94 ± 0.01 b 0.96 ± 0.01 c 0.89 ± 0.01 d Threonine 0.79 ± 0.02 a 0.92 ± 0.01 b 1.04 ± 0.01 c 0.83 ± 0.01 d Arginine 1.02 ± 0.01 a 1.19 ± 0.01 b 1.30 ± 0.01 c 1.03 ± 0.01 d Phenylalanine 0.67 ± 0.01 a 0.93 ± 0.01 b 1.05 ± 0.01 c 0.77 ± 0.01 d Tyrosine 0.43 ± 0.01 a 0.53 ± 0.00 b 0.57 ± 0.06 c 0.45 ± 0.01 d Methionine 0.18 ± 0.01 a 0.26 ± 0.01 b 0.30 ± 0.01 c 0.21 ± 0.01 d Histidine 0.40 ± 0.01 a 0.50 ± 0.01 b 0.57 ± 0.01 c 0.43 ± 0.01 d Tryptophan 0.06 ± 0.01 a 0.11 ± 0.01 b 0.07 ± 0.00 bc 0.09 ± 0.01 bd NEAA Alanine 0.85 ± 0.01 a 0.94 ± 0.01 b 0.87 ± 0.06 c 0.97 ± 0.01 bd Serine 1.01 ± 0.01 a 1.12 ± 0.01 b 1.23 ± 0.01 c 1.01 ± 0.01 d Glycine 1.15 ± 0.01 a 1.32 ± 0.01 b 1.29 ± 0.01 c 1.19 ± 0.01 d Proline 1.01 ± 0.01 a 1.05 ± 0.01 b 1.03 ± 0.01 c 1.03 ± 0.02 d Aspartic acid 1.25 ± 0.01 a 1.50 ± 0.01 b 1.40 ± 0.01 c 1.56 ± 0.01 d Glutamic 2.15 ± 0.03 a 2.88 ± 0.03 b 2.59 ± 0.01 c 3.01 ± 0.03 d Cystine 0.09 ± 0.01 a 0.07 ± 0.01 b 0.04 ± 0.01 c 0.09 ± 0.01 ad ∑EAA 7.56 ± 0.003 a 8.70 ± 0.003 b 9.03 ± 0.003 c 8.04 ± 0.003 d ∑NEAA 7.51 ± 0.008 a 8.88 ± 0.007 b 8.88 ± 0.004 c 8.84 ± 0.008 d ∑AA 15.07 ± 0.004 a 17.58 ± 0.002 b 17.91 ± 0.00 c 16.88 ± 0.003 d * Values represent the means of triplicate samples. The levels of free amino acids in the diets supplemented with different formulated products with a dosage of 150 ml/kg of feed are presented in Table 1 . All types of amino acids in the diets of KP1, KP2, KP3, and KP4 were significantly different ( P 0.05) between KP2, KP3, and KP4. Among the essential amino acids, leucine and arginine were found in the highest amounts in the KP1, KP2, KP3, and KP4 diets. There was no significant difference ( P > 0.05) in the alanine content between KP2 and KP3 diets and the cystine level in KP1 and KP3 diets. Of the nonessential amino acids, glutamic and aspartic acid represented a significant portion of all four diets. The present study found significant differences in the overall free essential and nonessential amino acid pools in the KP1, KP2, KP3, and KP4 diets ( Table 1 ). One-way ANOVA results exhibited a marginally significant interaction between experimental diets in terms of essential amino acids (F (3,8) = 11.371, P = 0.003), nonessential amino acids (F (3,8) = 0.407, P = 0.752), and overall amino acid pools (essential plus nonessential) (F (3,8) = 7.355, P = 0.011). Duncan's Post-hoc test revealed that the free essential amino acids (9.03 ± 0.003%), nonessential amino acids (8.88 ± 0.004%), and overall amino acid pools (17.91%) were significantly higher ( P < 0.05) in feed supplemented with CP3 products, followed by CP2, CP4, and CP1 products ( Table 1 ). Proximate and amino acid profile of the whole body of giant gourami Commercial feed combined with a new formulation product significantly affected the proximate carcass composition of juvenile giant gourami. One-way ANOVA results showed a marginal interaction among group treatments in the case of protein contents (F (3,8) = 1.522, P = 0.282), fat (F (3,8) = 5.663, P = 0.022), carbohydrates (F (3,8) = 1.862, P = 0.214), and crude fibre (F (3,8) = 1.445, P = 0.300). Duncan's Post-hoc test revealed that the protein content (28.85 ± 0.45%), fat (2.67 ± 0.04%), carbohydrates (1.97 ± 0.09%), and crude fibre (0.83 ± 0.02%) were significantly higher ( P 0.05) between treatments. For the energy total, KP3 was significantly higher ( P < 0.05) than the other treatments ( Table 2 ). However, the moisture content of the carcass did not show any significant variation among the KP1, KP2, KP3, and KP4 diets. Table 2. Whole-body proximate and amino acid composition of giant gourami after a 90-day feeding trial. Mean ± SD * . KP1 KP2 KP3 KP4 %, dry wet basis Proximate composition Dry matter 64.59 ± 0.16 a 64.51 ± 0.34 a 64.14 ± 0.33 a 64.24 ± 0.12 a Crude protein 28.64 ± 0.28 a 28.07 ± 0.79 ab 28.85 ± 0.45 c 28.66 ± 0.44 ad Crude fat 2.79 ± 0.03 a 2.88 ± 0.02 b 2.67 ± 0.04 c 3.00 ± 0.02 d Carbohydrate 1.38 ± 0.01 a 1.99 ± 0.06 b 1.97 ± 0.09 c 1.31 ± 0.02 d Crude fibre 0.97 ± 0.02 a 0.68 ± 0.01 b 0.83 ± 0.02 c 0.95 ± 0.04 d Ash 1.63 ± 0.02 a 1.70 ± 0.02 b 1.54 ± 0.01 c 2.11 ± 0.04 d Energy total (kg calorie/100 g) 144.77 ± 1.58 a 155.48 ± 1.26 b 157.90 ± 0.91 c 149.60 ± 0.29 d Amino acid composition EAA Leucine 2.13 ± 0.01 a 2.37 ± 0.01 b 2.42 ± 0.01 c 2.26 ± 0.01 d Isoleucine 1.13 ± 0.01 a 1.25 ± 0.01 b 1.38 ± 0.01 c 1.19± 0.01 d Lysine 2.77 ± 0.01 a 3.16 ± 0.02 b 3.88 ± 0.01 c 2.86 ± 0.01 d Valine 1.26 ± 0.01 a 1.40 ± 0.01 b 1.32 ± 0.01 c 1.35 ± 0.01 d Threonine 1.38 ± 0.02 a 1.49 ± 0.01 b 1.43 ± 0.01 d 1.48 ± 0.01 d Arginine 1.58 ± 0.01 a 1.71 ± 0.01 b 1.63 ± 0.01 c 1.70 ± 0.01 d Phenylalanine 1.02 ± 0.01 a 1.11 ± 0.01 b 1.08 ± 0.01 c 1.11 ± 0.01 d Tyrosine 0.80 ± 0.01 a 0.84 ± 0.00 b 0.83 ± 0.01 c 0.85 ± 0.06 d Methionine 0.15 ± 0.01 a 0.21 ± 0.01 b 0.18 ± 0.01 c 0.16 ± 0.01 d Histidine 0.55 ± 0.01 a 0.56 ± 0.01 ab 0.59 ± 0.01 c 0.57 ± 0.01 d Tryptophan 0.08 ± 0.01 a 1.02 ± 0.01 b 1.08 ± 0.01 c 0.06 ± 0.00 d NEAA Alanine 1.86 ± 0.01 a 2.08 ± 0.01 b 2.92± 0.01 c 1.97 ± 0.01 d Serine 1.28 ± 0.01 a 1.31 ± 0.01 b 1.26 ± 0.01 c 1.31 ± 0.01 d Glycine 1.58 ± 0.01 a 1.68 ± 0.01 b 1.61 ± 0.01 c 1.77 ± 0.01 d Proline 1.06 ± 0.01 a 1.16 ± 0.01 b 1.08 ± 0.01 c 1.16 ± 0.01 d Aspartic acid 2.71 ± 0.01 a 3.08 ± 0.01 b 3.79± 0.01 c 2.77 ± 0.01 d Glutamic 4.36 ± 0.03 a 4.92 ± 0.01 b 4.97± 0.01 c 4.66 ± 0.01 d Cystine 0.06 ± 0.01 a 0.09 ± 0.01 b 0.06 ± 0.01 c 0.05 ± 0.01 d ∑EAA 12.68 ± 0.003 a 15.13 ± 0.005 b 15.82± 0.001 c 13.61 ± 0.008 d ∑NEAA 12.91 ± 0.007 a 14.32 ± 0.01 b 15.69 ± 0.002 c 13.50 ± 0.001 d ∑AA 25.59 ± 0.003 a 29.45 ± 0.04 b 31.51 ± 0.001 c 27.11 ± 0.004 d * Values represent the means of triplicate samples. The mean quantities of total amino acids in the carcasses of O. goramy fed different diets are given in Table 2 . Lysine and leucine represented a significant portion of the essential amino acids of the whole body carcass, and methionine was present in small quantities in all of the whole-body meat. Of the nonessential amino acids, glutamic acid, aspartic acid, and alanine were the highest, and cystine was the lowest for all whole-body carcasses of giant gourami fed different diets. The levels of glutamic acid were significantly higher in carcasses of fish fed the KP3 diet than in those provided the KP1, KP2, and KP4 diets. When the overall quantities of total essential and nonessential amino acids were compared, the whole-body carcass amino acid content was significantly lower ( P < 0.05) in fish fed the KP1 diet than in those fed the KP2, KP3, and KP4 diets ( Table 2 ). The number of amino acids (essential plus nonessential) in the carcasses of fish fed the KP3 diet was significantly higher than that in fish fed the KP1, KP2, and KP4 diets. Growth coefficient and survival The growth coefficient and feed utilization of the giant gourami juveniles displayed significant differences among the diets. One-way ANOVA results exhibited a marginally significant difference between experimental diets in the case of the thermal unit growth coefficient (F (3,8) = 153.99, P = 0.458), and daily growth coefficient (F (3,8) = 59.88, P = 0.288), while total feed intake (% BW day-1) (F (3,8) = 14.938, P = 0.56), and protein efficiency ratio (F (3,8) = 15.78, P = 0.29) also showed significant differences ( P < 0.05) among the treatment diets ( Figure 2 ). Figure 2. Growth coefficient and feed utilization of the giant gourami juveniles reared under different diets during 90 days of the experiment period. (A) Thermal growth coefficient (TGC), (B) daily growth coefficient (DGC), (C) feed intake (FI), and (D) protein efficiency ratio (PER). The mean value and standard deviation (mean ± SD) are presented for giant gourami (n = 3). Different superscripts in the bar diagram of the giant gourami juvenile TGC, DGC, FI, and PER indicate significant differences among other diets ( P < 0.05, One-way ANOVA Duncan Post-Hoc). Furthermore, the thermal growth coefficient (TGC) has often been used to predict growth performance and production performance of aquaculture using water temperature at the fish-rearing location. This study presents the relationship between thermal growth coefficient and condition factor, daily growth coefficient, and protein efficiency ratio ( Figure 3 ). The thermal growth coefficient had strong relationships with the condition factor ( r 2 = 0.777, Figure 3A ), daily growth coefficient ( r 2 = 0.920, Figure 3B ), and protein efficiency ratio ( r 2 = 0.749, Figure 3D ), while the thermal growth coefficient had a moderate relationship with the feed intake ( r 2 = 0.698, Figure 3C ). Figure 3. Relationships between thermal growth coefficient and condition factor (A), daily growth coefficient (B), feed intake (C) and protein efficiency ratio (D) for giant gourami ( O. gourami ) over 90 days. Condition factor and body indices of giant gourami after 90 days of feeding The condition factor was significantly different between diets (F (3,8) = 19.98, P = 0.566) in the present study; while the GSI, HIS, and VFSI displayed marginally significant differences between diets. The HIS was significantly (F (3,8) = 5.389, P = 0.500) higher in the KP3 diet, but KP1, KP2, and KP4 diets had no significant differences among them ( Table 3 ). The GSI value of giant gourami was significantly (F (3,8) = 10.492, P = 0.243) different between diets, and the GSI of giant gourami fed KP3 rations was higher than if fed KP1, KP2, or KP4 diets. The VFSI was not considerably different among the KP1, KP2, and KP4 diets. The Duncan's post-hoc test revealed that the HIS (1.30 ± 0.13%), GSI (4.15 ± 0.36%), and VFSI (2.75 ± 0.34%) were significantly higher ( P 0.05) among the treatment diets ( Table 3 ). Table 3. Mean (± SD) value condition factor and body indices of giant gourami during the 90-day experimental period. Growth coefficients KP1 KP2 KP3 KP4 Condition factor (CF) 2.45 ± 0.09 a 2.90 ± 0.07 b 2.92 ± 0.13 c 2.61 ± 0.04 d Viscerosomatic index (GSI%) 3.20 ± 0.21 a 3.77 ± 0.09 b 4.15 ± 0.36 c 3.17 ± 0.02 d Hepatosomatic (HIS%) 0.97 ± 0.05 a 1.06 ± 0.19 ab 1.30 ± 0.13 c 1.04 ± 0.12 ad Visceral fat-somatic indexes (VFSI%) 2.15 ± 0.13 a 2.29 ± 0.22 ab 2.75 ± 0.34 c 1.74 ± 0.21 ad Bilesomatic (BSI%) 10.11 ± 0.76 10.58 ± 1.01 10.48 ± 1.28 10.29 ± 0.77 Gut micromorphology The gut morphometric measurements of giant gourami juveniles are presented in Table 4 . Fish gut micromorphology was significantly affected by different feeds. One-way ANOVA results showed a significant effect of feed differences between groups in terms of foregut fold height (F (3.8) = 816.70, P = 0.135), foregut fold width (F (3.8) = 129.34, P = 0.974), height of the foregut (F (3.8) = 169,80, P = 0.882), and microvillus height of the foregut (F (3.8) = 56,01, P = 0.285). The Duncan's post-hoc test demonstrated that the foregut fold height (434.13 ± 1.76 μm), fold width (53.23 ± 0.88 μm), enterocyte height (27.42 ± 0.42 μm), and microvillus height (2.79 ± 0.45 μm) were significantly higher ( P < 0.05) in fish fed the KP3 diet than those fed the other diets. For the midgut, one-way ANOVA results showed a significant interaction among treatments in the case of fold height (F (3,8) = 5602.628, P = 0.055), fold width (F (3,8) = 129.341, P = 0.974), enterocyte height (F (3,8) = 169.809, P = 0.882), and microvillus height (F (3,8) = 56.016, P = 0.285). The Duncan's post-hoc test showed that the fold height of the midgut (324.96 ± 1.43 μm), fold width (61.50 ± 1.02 μm), and enterocytes (32.82 ± 0.54 μm) were significantly higher ( P < 0.05) in fish fed the KP3 diet, whereas microvillus height was significantly higher in fish fed the KP2 diet ( Table 4 ). Fish fed the KP3 diet showed a higher fold height of the hindgut (F (3,8) = 5459.01, P = 0.066), fold width (F (3,8) = 271.94, P = 0.865), enterocyte height (F (3,8) = 299.180, P = 0.821), and microvillus height (F (3,8) = 253.57, P = 0.316) ( Figure 4 ). Table 4. Gut micromorphology of giant gourami juveniles fed different diets for 90 days. Foregut Midgut Hindgut hF (μm) a wF (μm) b hE (μm) c hMV (μm) d hF (μm) wF (μm) hE (μm) hMV (μm) hF (μm) wF (μm) hE (μm) hMV (μm) KP1 336.17 ± 5.59 a 51.30 ± 0.85 a 26.21 ± 0.43 a 2.56 ± 0.45 a 227.50 ± 0.16 a 47.16 ± 0.78 a 24.31 ± 0.31 a 1.64 ± 0.03 a 213.92 ± 0.19 a 42.91 ± 0.59 a 20.22 ± 0.25 a 1.49 ± 0.02 a KP2 343.43 ± 1.38 b 52.14 ± 0.86 b 26.84 ± 0.44 b 2.77 ± 0.45 b 274.61 ± 1.21 b 58.12 ± 0.97 b 29.87 ± 0.49 b 1.85 ± 0.01 b 243.51 ± 1.07 b 53.01 ± 0.88 b 28.00 ± 0.46 b 1.64 ± 0.01 b KP3 434.13 ± 1.76 c 53.23 ± 0.88 a 27.42 ± 0.42 c 2.79 ± 0.45 c 324.96 ± 1.43 c 61.50 ± 1.02 c 32.82 ± 0.54 c 1.80 ± 0.03 c 305.60 ± 1.35 c 60.02 ± 0.99 c 29.54 ± 0.49 c 1.77 ± 0.02 c KP4 321.18 ± 1.42 d 50.20 ± 0.83 d 25.62 ± 0.79 d 2.31 ± 0.07 d 228.45 ± 1.01 d 56.95 ± 0.95 d 29.19 ± 0.48 d 1.69 ± 0.01 d 217.69 ± 0.96 d 61.64 ± 1.03 d 24.32 ± 24.32 d 1.40 ± 0.01 d a hF = fold height. b wF = fold width. c hE = enterocyte height. d hMV = microvillus height. Figure 4. Light microscopy of the foregut (A), midgut (B) and hindgut (C) morphology of juvenile giant gorami of sago strain showing fold height (hF), enterocyte height (hE), and microvillus height (hMV) in fish fed KP1, KP2, KP3, and KP4 over 90 days. (Scale bar: 500μm; magnification ×100). Pond water quality The pond water quality values of the giant gourami juvenile rearing freshwater concrete pond were recorded; water temperatures, dissolved oxygen (DO), total alkalinity, hardness, pH, and nitrates were in the range of typical values as given by WHO/FAO, as shown in Table 5 . Table 5. The average values and range of water quality parameters in the concrete pond during the 90-days of experiment. Water quality parameters n Mean ± SD Range WHO/FAO limits References Water temperatures (°C) 45 28.01 ± 1.06 27–30 25–33 Prokoso et al. 43 Dissolved oxygen (mg/L) 14 6.01 ± 0.14 5.80–6.20 3–5 Syandri et al. 44 Total alkalinity (mg/L as CaCO 3 ) 14 58.09 ± 3.33 52.5–62.5 120 Boyd et al. 45 Hardness (mg/L as CaCO 3 ) 14 66.34 ± 1.32 65–68.5 168 Boyd et al. 45 pH 14 7.48 ± 0.19 7.2–7.8 6.5–9.0 Boyd et al. 45 Nitrates (mg/L) 14 0.04 ± 0.01 0.03–0.05 0.2–219 Boyd and Tucker 46 Discussion The chemical analysis of fish feed is essential because it provides valuable information to aquafeed nutritionists concerned with readily available sources of proximate and amino acid compositions, including minerals and vitamins. This study investigated the nutritional quality of fish feed enriched with three different formulation products and one as a placebo. Dietary protein levels for giant gourami ranged from 19.68 to 21.70%. Overall, the crude protein content in the feed of this study was within the ranges observed by other authors. 47 – 49 The giant gourami belongs to the trophic level of herbivorous fish. 50 Generally, herbivorous fish require a lower dietary protein level than carnivorous fish. 51 , 49 Reducing the protein content of aquafeed is one method to increase continuous fish farming, by diminishing feed costs and reducing the impact on the aquatic environment. 2 , 52 The fat content of the feed ranged from 3.41 to 3.67%, which is similar to the feed fat content for juvenile grass carp, Ctenopharyngodon idella , 53 and lower than the feed fat content for the herbivorous fish Ancistrus cirrhosis 48 and for rearing rohu, Labeo rohita . 54 At the same time, the carbohydrate content of all feed treatments ranged from 26.37 to 31.19%, and the energy total (kg calorie/100 g) was between 234.41 and 240.87. Although protein content as an energy source for the maintenance and growth of giant gourami is relatively low, energy can be acquired from either protein or nonprotein sources, i.e. , fat and carbohydrates. In the present study, the commercial fish feed was enriched with natural sources, i.e. , formulated products of mature coconut water and palm sap sugar fermented with various fungi ( Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae ). In the recent past, the dose used was 300 ml/kg of feed. This method is a new approach that has been developed by Azrita et al. 9 to improve feed nutrition and whole-body carcasses, covering fatty acids, the atherogenic index and thrombogenic, feed efficiency, and growth performance of giant gourami. Here, we continued the investigation by reducing the feed dose to 150 ml/kg. This study's results found that supplementing feed with newly formulated products can increase feed nutrition, covering amino acids in diet and body meat, and the growth coefficient of giant gourami. Several authors have reported increasing feed nutrition and maximizing the digestive enzyme activity of aquacultured fish by providing feed supplemented with EPA and DHA, 17 iodine and selenium, 10 methionine, 12 fish oil, 19 , 11 and soybean oil. 20 In addition, the provision of feed has been supplemented with probiotics, 21 glycine, and prebiotics. 22 In this study, mature coconut water and palm sap sugar solution fermented with various fungi were used to supplement fish feed. In addition to coconut water and palm sugar, mushrooms also play a role in increasing feed nutrition. However, it's better to use Rhizopus oligosporus. As in the present study, Varzakas 55 and Vong et al. 56 showed that Rhizopus oligosporus can produce various extracellular enzymes. Aspergillus niger. has a high capacity to degrade antigenic proteins, including carbohydrases, proteases, lipases, and phosphatases, when used for fermenting plant-sourced fish feed ingredients. 12 , 57 Saccharomyces cerevisiae is one of the most acclaimed microorganisms. Its effectiveness is due to its useful composition, such as “β-glucans, nucleic acids, mannan oligosaccharides and chitin,” which are used for fermented ingredients. 7 , 58 The amino acid composition can be used to assess feed quality. Leucine, arginine, and glutamic acid were the most abundant free amino acids in the KP1, KP2, KP3, and KP4 diets. Similarly, in other studies on fish feed, such as feed for largemouth bass, Micropterus salmoides , the feeds were supplemented with glycine, prebiotics, and nucleotides in a soybean meal-based diet. 22 Feed for pacu, Piaractus mesopotamicus, was supplemented with an essential amino acid, 59 and feed for snubnose pompano, Trachinotus blochii , was supplemented with different levels of protein. 60 Apparently, supplementing feed with different ingredients is common, and in other species, leucine, arginine, and glutamic acid were the most abundant FAAs. Conversely, methionine levels were low in all experimental feeds. Methionine is one amino acid that must be available in fish feed because methionine is needed to protect body cells from stress. For optimal growth of juvenile hybrid grouper, 1.89% methionine is required in the feed. 18 The experimental feed contained 0.18–0.30% methionine, but whether this amount is sufficient for the needs of giant gourami is poorly understood. In the current study, the nonessential amino acid compositions were slightly higher than the essential amino acid compositions in all the experimental diets. It was higher in the KP3 diet than the other diets. In contrast, the essential amino acids of fish feed for snubnose pompano were slightly higher than the nonessential amino acids content. 60 This difference may be caused by differences between freshwater fish and marine fish. As in the present study, Prabu et al. 60 reported that different dietary protein levels also caused different pools of FAAs, including limiting essential amino acid types in the diet 59 and supplemental glycine, prebiotic, and nucleotide levels in the soybean meal-based diet. 22 In the present study, this difference in FAA content is caused by various mushrooms used in the formulated products. Giant gourami juveniles fed the KP3 diet showed higher levels of glutamic acid, aspartic acid, leucine, and lysine and lower levels of tyrosine, methionine, histidine, tryptophan, and cystine in their carcasses than those fed other diets. The carcasses of giant gourami fed the KP3 diet showed the highest sum of FAAs compared to cultured fish fed the KP1, KP2, and KP4. The differences in the FAA profile in the whole-body carcasses of giant gourami could be related to the fungus type used in the formulated products for enriched feed. Each type of mushroom has a different function depending on the fermented fish feed ingredients and is correlated with the whole-body carcass amino acids. 12 , 57 The FAA profile differences could be related to different aspects, such as diet composition, 61 dietary protein level, 62 and methionine levels in the diet, 18 including the water quality of the ponds. 63 This study does not analyse the relationship between growth performance and FAA profile or pond water quality. Several authors have reported that the physiological parameters of water quality and animal body composition are usually interrelated. 64 , 60 The present study did not examine whether the difference in FAAs in the whole-body carcass is correlated to pond water quality. The lower weight gain of fish fed the KP1 diet compared to fish fed the KP2, KP3, and KP4 diets shows that a deficiency of either fungus in the formulated product for the enriched diet could lower the protein content and related sum amino acids, leading to the inhibition of giant gourami growth. In addition, it also affects feed intake and feed conversion ratios. The low protein efficiency ratio and daily growth coefficient in fish provided the insufficient KP1 diet were perhaps due to an amino acid imbalance. The amino acid content of the KP2, KP3, and KP4 diets increased, ranging from 16.88% to 17.91% after fermentation. The increase may be due in part to the increased protein content in the KP2, KP3, and KP4 diets, which was in line with the results of Jannatullah et al. 57 and Li et al. , 12 who found that Aspergillus niger and Aspergillus awamori fermentation increased the amino acid content of soybean meal by 2.56% and 15.56%, respectively. In addition, Dawood et al. 36 stated that the essential amino acid profile was changed after fermentation by Saccharomyces cerevisiae. This might result from the different fungi used having different utilization patterns for amino acids in this study. It influences the growth performance and nutrient utilization of giant gourami juveniles. We found that the methionine proportion was lower in the diets in the current study. In addition, methionine is an essential amino acid that plays a unique role in protein structure and metabolism. 18 It is possible that Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae fermentation promoted the conversion of specific amino acids to methionine. However, the exact mechanisms need to be studied further. In the present study, the thermal growth coefficient (TGC) strongly correlated with the daily growth coefficient (DGC). Because faster daily fish growth requires a quality diet and constant water temperature during the rearing period, in this study, water temperature ranged from 27 to 30°C, and dissolved oxygen was between 5.8 and 6.2 mg/L. According to Besson et al. , 65 higher daily energy availability in the diet can lead to faster-growing fish, which is supported by constant water temperature and higher daily oxygen levels. The thermal growth coefficient had an essential change in environmental value. 66 Therefore, it was very important to keep the water temperature and dissolved oxygen constant in the aquaculture locations. At the same time, 78% of TGC values were determined by the condition factor connected to whole body weight and the total fish length. TGC of Atlantic cod, Gadus morhua , is influenced by body size and condition factors. 67 In this study, a higher value of TGC was detected in fish fed KP3; the effect is that the daily growth coefficient, and the protein efficiency ratio is better. Conversely, decreasing TGC has two effects, i.e. , a slow fish growth and lowered daily feed intake. Many scientists state that in aquaculture operations, net yield (kg/m 3 ) depends upon TGC fluctuation, feed intake, and daily oxygen consumption. 65 , 68 , 69 In the present study, food enrichment with different formulated products did not affect HIS or VFSI except in the KP3 diet. Whereas GSI is influenced by differences in diet, it did not affect BSI. The condition factor of largemouth bass, Micropterus salmoides (1.49–1.52%), fed enriched 1–2% EPA + DHA 17 was different from the value (0.68) reported by Arriaga-Hernandez et al. 70 for white snook ( Centropomus viridis ) juveniles fed a 15% replacement of fish meal with soybean meal. Moreover, Hassan et al. 71 reported condition factor values ranging from 1.52 to 2.95 and an HSI between 1.4 and 1.5 for Lates calcarifer under different feeding rates (3–9% body weight d -1 ). Barbosa et al. 72 reported VSI and LSI values of 2.24 and 3.86, respectively, for Centropomus parallelus fed a commercial diet. On the other hand, Syed et al. 64 also reported HSI and VSI values of 3.41 and 4.90, respectively, for Oreochromis niloticus at different levels of aloe vera extract as feed additives. In our study, the VSI of giant gourami ranged from 3.17 to 4.15, and the LSIs were between 1.74 and 2.75, both higher than those recorded at different stocking densities of giant gourami. 44 The high content of visceral fat observed in fish fed the KP3 diet might be explained by the diet having fat contents that exceed the needs of giant gourami juveniles and by the reduced energy expenditure of fish that are confined to rearing frame nets. Therefore, further analysis is necessary to determine the optimum dosage of the formulated product for the enrichment of feed to improve the growth performance of giant gourami. For fish, the gut plays a significant role in absorbing nutrients, which is closely related to feed utilization. 18 , 73 Rossi et al. 22 demonstrated that the development of enterocytes affected the nutrient-absorbing efficiency of the gut of Micropterus salmoides. Feeding Lates calcarifer juveniles with the same basal diet supplemented with 1% probiotic yeast, Saccharomyces cerevisiae , and lactic acid bacteria, Lactobacillus casei, revealed a higher number of gut mucosal goblet cells and increased microvillous length. 74 In contrast, substituting as much as 12.5–25% soya protein concentrates with lupin ( Lupinus albus ) meal in carp ( Cyprinus carpio ) diets does not significantly affect the villi length and villi width of the gut. 75 In the current study, enriched feed with products supplemented from coconut water, palm sap sugar, and fungus significantly affected the micromorphology and gut size. The fold height, fold width, enterocyte height, and microvilli of fish fed the KP3 diet were higher than those of fish fed the KP1, KP2, and KP4 diets. The KP3 diet is a relevant formulated product to enrich commercial feed to promote the development of the gut in animal experiments, which may somewhat describe the significant growth performance and feed efficiency used in this study. Furthermore, the micromorphology gut size of giant gourami is smaller than that of juvenile hybrid grouper, 18 turbot, Scophthalmus maximus , 12 largemouth bass, Micropterus salmoides , 22 and common carp, Cyprinus carpio . 75 The trophic food habits of fish may also affect the gut's hF, wF, hE, and hMV size because these habits are correlated with the digestibility coefficient. Under natural conditions, giant gourami is an herbivorous fish, while grouper, largemouth bass, and turbot are predatory fish, and common carp are omnivorous. Whether giving fish from different trophic levels the same diet affects the size of gut hF, wF, hE, and hMV is poorly understood. Conclusions The present investigation observed that feed enriched with newly formulated products made from mature coconut water and palm sap sugar, and fermented with various mushrooms, given to fish in a dose of 150 ml/kg substantially affected the amino acid composition of the diet and whole-body carcass of giant gourami juveniles. It also affected the growth coefficient, feed utilization, body indices, and gut micromorphology size. The thermal growth coefficient had a strong relationship with the daily growth coefficient ( r 2 = 92%), condition factor ( r 2 = 77%), and protein efficiency ratio ( r 2 = 75%), while a moderate relationship with the feed intake ( r 2 = 69%). The CP3 formulation was optimal for feed quality, and the KP3 diet was optimal for body carcass, growth coefficient, body indices, and the ability of the intestines for feed absorption. Thus, our study also informs fish farmers about culturing good quality giant gourami and fulfilling nutrition requirements for food security. Data availability Underlying data Figshare: Underlying data for ‘Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles’. https://doi.org/10.6084/m9.figshare.20407647 . 76 This project contains the following underlying data: - Table 1. Raw data of the experimental diets’ proximate composition - Table 2. Raw data of amino acid of feed experimental - Table 3. Raw data of whole body carcass proximate composition - Table 4. Raw data of amino acid of whole-body carcass - Table 5. Daily growth coefficient, feed utilization and body indices of giant gourami after 90 days of feeding. - Table 6. Raw data gut micromorphology of giant gourami juveniles fed different diets for 90 days Data are available under the terms of the Creative Commons Attribution 4.0 International License (CC-BY 4.0). Acknowledgments We are grateful to the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia for funding this research. We would also like to thank the students and fish farmers who helped with data collection in the field and in the laboratory. References 1. Food and Agriculture Organization (FAO): The state of world fisheries and aquaculture: Opportunities and challenges. Food and Aqriculture Organization of the United Nations; 2018; 209. 2. Hua K, Cobcroft JM, Cole A, et al. : The future of aquatic protein: Implications for protein sources in aquaculture diets. One Earth. 2019; 1 (3): 316–329. Publisher Full Text 3. Gòmez B, Munekata PES, Zhu Z, et al. : Challenges and opportunities regarding the use of alternative protein sources: Aquaculture and Insects. Adv. Food Nutr. Res. 2019; 89 : 259–295. PubMed Abstract | Publisher Full Text 4. Yuru Z, Ronghua L, Chaobin Q, et al. : Precision nutritional regulation and aquaculture. Aquac. Rep. 2020; 18 : 100496. 5. Nagappan S, Das P, AbdulQuadir M, et al. : Potential of microalgae as a sustainable feed ingredient for aquaculture. J. Biotechnol. 2021; 341 : 1–20. PubMed Abstract | Publisher Full Text 6. Hasimuna O, Maulu S, Monde C, et al. : Cage aquaculture production in Zambia: Assessment of opportunities and challenges on Lake Kariba Siavonga district. Egypt. J. Aquat. Res. 2019; 45 : 281–285. Publisher Full Text 7. Dawood MAO, Koshio S: Application of fermentation strategy in aquafeed for sustainable aquaculture. Rev. Aquac. 2019; 12 : 987–1002. Publisher Full Text 8. Junaidi, Syandri H, Azrita, et al. : Floating cage aquaculture production in Indonesia: Assessment of opportunities and challenges in Lake Maninjau. AIMS. Environ. Sci. 2022; 9 (1): 1–15. 9. Azrita, Syandri H, Aryani N, et al. : The utilization of new products formulated from water coconut, palm sap sugar, and fungus to increase nutritional feed quality, feed efficiency, growth, and carcass of gurami sago, Osphronemus goramy (Lacepède, 1801) juvenile. F1000Res. 2021; 10 : 1121. Publisher Full Text 10. Barbosa V, Maulvault AL, Anacleto P, et al. : An enriched feed with iodine and selenium from natural and sustainable sources to modulate farmed gilthead seabream ( Sparus aurata ) and common carp ( Cyprinus carpio ) fillets elemental nutritional value. Food Chem. Toxicol. 2020; 140 : 111330. PubMed Abstract | Publisher Full Text 11. Duarte FOS, Faula FG, Prado CS, et al. : Better fatty acid profile in fillets of Nile tilapia ( Oreochromis niloticus ) supplemented fish oil. Aquaculture. 2020; 534 : 736241. Publisher Full Text 12. Li X, Wu X, Dong Y, et al. : Effects of dietary levels on growth, feed utilization and related gene expression of juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinenephelus lanceolatus ♂). Aquaculture. 2019; 502 : 153–161. Publisher Full Text 13. Nagarajan D, Varjani S, Lee D, et al. : Sustainable aquaculture and animal feed from microalgae: Nutritive value and techno-functional components. Renew. Sust. Energ. Rev. 2021; 150 : 111549. Publisher Full Text 14. Lall SP, Dumas A: Nutritional requirements of cultured fish. Feed and Feeding Practices in Aquaculture. 2015; 53–109. Publisher Full Text 15. Mohanty BP, Mahanty A, Ganguly S, et al. : Nutritional composition of food fishes and their importance and providing food and nutritional security. Food Chem. 2019; 293 : 561–570. PubMed Abstract | Publisher Full Text 16. Beltrȃn JMG, Esteban MA: Nature-identical compounds as feed additives in aquaculture. Fish Shellfish Immunol. 2022; 123 : 409–416. PubMed Abstract | Publisher Full Text 17. Yadav AK, Rossi W, Habte-Tsion, et al. : Impacts of dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) level and ratio on the growth, fatty acid composition, and hepatic-antioxidant status of largemouth bass ( Micropterus salmoides ). Aquaculture. 2020; 529 : 735683. Publisher Full Text 18. Li X, Mu W, Wu X, et al. : The optimum methionine requirement in diet of juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂): Effects on survival, growth performance, gut micromorphology and immunity. Aquaculture. 2020; 520 : 735014. Publisher Full Text 19. Aryani N, Azrita, Mardiah A, et al. : Influence of feeding rate on the growth, feed efficiency, and carcass composition of the giant gourami ( Osphronemus goramy ). Pakistan. J. Zool. 2017; 49 (5): 1775–1781. 20. Godoy AC, Oliveira SO, Oxford JH, et al. : Soybean oil for Nile tilapia ( Oreochromis niloticus ) in finishing diets: Economic, zootechnical and nutritional meat improvements. Aquaculture. 2019; 512 : 734324. Publisher Full Text 21. Ringø E: Probiotics in shellfish aquaculture. Aquac. Fish. 2020; 5 (1): 1–27. Publisher Full Text 22. Rossi W, Allen KM, Habte-Tsion H-M, et al. : Supplementation of gycine, prebiotic, and nucleotides in soybean meal-based diets for largemouth bass ( Micropterus salmoides ): Effects on production performance, whole-body nutrient composition and retention, and intestinal histopathology. Aquaculture. 2021; 532 : 736031. Publisher Full Text 23. Afriyanti EK, Hasan ODS, Djunaidi IS: Growth performance of giant gourami, Osphoronemus goramy fed with a combination of fish meal and Azolla flour, Azolla microphyll. Jurnal Iktiologi Indonesia. 2020; 20 (2): 114–133. Publisher Full Text 24. Andriani J, Setiawati M, Sunarno MTJ: Diet digestibility and growth performance of giant gourami juvenile, Osphronemus goramy fed on a diet supplemented using glutamine. Jurnal Iktiologi Indonesia. 2019; 19 (1): 1–11. Publisher Full Text 25. Budi DS: Alimuddin, Suprayudi MA: Growth response and feed utilization of giant gourami ( Osphronemus goramy ) juvenile feeding different protein levels of the diets supplemented with recombinant growth hormone. HAYATI J. Biosci. 2015; 22 (1): 12–19. Publisher Full Text 26. Wibowo WP, Sumidjan I, Rachmawati D: Analysis of relative growth rate, feed efficiency and survival of giant gourami, Osphronemus goramy juvenile through substituting chicken feather flour silage in artificial feed. J. Aquac. Manag. Technol. 2017; 6 (2): 51–58. 27. Segura-Badilla O, Lazcano-Hernández M, Kammar-García A, et al. : Use of coconut water ( Cocus nucifera L) for the development of a symbiotic functional drink. Heliyon. 2020; 6 (3): e03653. PubMed Abstract | Publisher Full Text | Free Full Text 28. Kumar M, Saini SS, Agrawal PK, et al. : Nutritional and metabolomics characterization of the coconut water at different nut developmental stages. J. Food Compos. Anal. 2021; 96 : 103738. Publisher Full Text 29. Paixâo LB, Brandâo GC, Araujo RGO, et al. : Assessment of cadmium and lead in commercial coconut milk employing HR-CS GF AAS. Food Chem. 2019; 284 : 259–263. PubMed Abstract | Publisher Full Text 30. Lakshmanan J, Zang B, Wright K, et al. : Tender coconut water suppresses hepatic inflammation by activating AKT and JNK signaling pathways in an in vitro model of sepsis. J. Funct. Foods. 2020; 64 : 103637. PubMed Abstract | Publisher Full Text | Free Full Text 31. Ho CW, Wan Aida WM, Maskat, et al. : Changes in volatile compounds of palm sap ( Arenga pinnata ) during the heating process for production of palm sugar. Food Chem. 2007; 102 : 1156–1162. Publisher Full Text 32. Saputro AD, de Walle DV , Dewettinck K: Physicochemical properties of coarse palm sup sugars as a natural alternative sweetener. Food Biosci. 2020; 38 : 100780. Publisher Full Text 33. Heryeni H: The virtues of palm sugar & product development strategy. Lambung Mangkurat University Press; 2016. (In Indonesian). 34. Sarma C, Mummaleti G, Sivanandham V, et al. : Anthology of palm sap: The global status, nutritional composition, health benefits & value added products. Trends Food Sci. Technol. 2022; 119 : 530–549. Publisher Full Text 35. Garrido-Galand S, Asensio-Grau A, Calvo-Lerna J, et al. : The potential of fermentation on nutrition and technological improvement of cereal and legume flours: A review. Int. Food Res. J. 2021; 145 : 110398. PubMed Abstract | Publisher Full Text 36. Dawood MAO, Eweedah NM, Khalafalla MM, et al. : Saccharomyces cerevisiae increases the acceptability of Nile tilapia ( Oreochromis niloticus ) to date palm seed meal. Aquac. Rep. 2020; 17 : 100314. Publisher Full Text 37. Zhang X, Peng L, Dai Y, et al. : Anti-cataract effects of coconut water in vivo and in vitro. Biomed. Pharmacother. 2021; 143 : 112032. PubMed Abstract | Publisher Full Text 38. Prades A, Dornier M, Diop N, et al. : Coconut water uses, composition and properties: a review. Fruits. 2012; 67 (2): 87–107. Publisher Full Text 39. AOAC: Official Methods of Analysis. 15th ed.Washington, DC., USA: Association of Official Analytical Chemists (AOAC); 1990. 40. Cohen SA: Amino acid analysis using pre-column derivatization with6-aminoquinolyl-N-hydroxysuccnimidyl carbomate. Protein Sequencing Protocols. 2nd Edn. vol. 211 . . Smith BJ, editor. Totowa, NJ: Humana Press Inc.; 2003; pp. 143–154. 9781592593422. Publisher Full Text 41. APHA: Standard Methods for Examination of Water and Wastewater. 19th ed.Washington DC, USA: American Public Health Association; 1995. 42. Duncan DB: Multiple ranges and multiple F tests. Biometrics. 1955; 11 : 1–42. Publisher Full Text 43. Prokoso VA, Pouil S, Chahyanti W, et al. : Fluctuating temperature regime impairs growth in giant gourami ( Osphronemus goramy ) larvae. Aquaculture. 539 : 736606. Publisher Full Text 44. Syandri H, Mardiah A, Azrita, et al. : Effect of stocking density on the performance of juvenile gurami sago ( Osphronemus goramy ) in the synthetic sheet pond. Pak. J. Zool. 2020; 52 (2): 717–726. 45. Boyd CE, Tucker CS, Somridhivej B: Alkalinity and hardness: Critical but elusive concepts in aquaculture. J. World Aquacult. Soc. 2016; 47 (1): 6–41. Publisher Full Text 46. Boyd CE, Tucker CS: Pond aquaculture water quality management. Boston: Kluwer Academic Publishers; 1988. 47. Timalsina P, Yadav CNR, Lamsal GP, et al. : Effect of stocking density and source of animal protein on growth and survival of rainbow trout fingerling in flow-through system at Nuwakot Nepal. Aquac. Rep. 2017; 8 : 58–64. Publisher Full Text 48. Vucko MJ, Cole AJ, Moorhead J, et al. : The freshwater macroalga Oedogonium intermedium can meet the nutritional requirements of the herbivorous fish Ancistrus cirrhosis. Algal. Res. 2017; 27 : 21–31. Publisher Full Text 49. Yu H, Liang H, Ren M, et al. : A study to explore the effects of low dietary protein levels on the growth performance and nutritional metabolism of grass carp ( Ctenopharigodon idella ) fry. Aquaculture. 2022; 546 : 737324. 50. Syandri H, Azrita, Sumiarsih E, et al. : Nutrient loading and farm characteristics of giant gourami fish aquaculture systems in Lake Maninjau, Indonesia: Basic knowledge of production performance. F1000 Res. 2021; 10 : 378. Publisher Full Text 51. Omasaki SK, Janssen K, Besson M, et al. : Economic values of growth rate, feed intake, feed conversion ratio, mortality and uniformity for Nile tilapia. Aquaculture. 2017; 481 : 124–132. Publisher Full Text 52. Arenas M, Alvarez-Gonzalez A, Barreto A, et al. : Effect of dietary carbohydrates on growth performance, feed efficiency and glucose metabolism in common snook ( Centropomus undecimalis ) and yellowtail snapper ( Ocyurus chrysurus ) juveniles. Aquaculture. 2021; 543 : 736958. Publisher Full Text 53. Tian J, Li Y, Zhang K, et al. : Dietary Pennisetum sinese Roxb meal reduces lipid accumulation and alleviates enteritis in juvenile grass carp, Ctenopharingodon Idella. Aquac. Rep. 2021; 21 : 1000915. 54. Ali S, Kaviraj A: Aquatic weed Ipomoea aquatica as a feed ingredient for rearing Rohu, Labeo rohita (Hamilton). Egypt. J. Aquat. Res. 2018; 44 (4): 321–325. Publisher Full Text 55. Varzakas T: Rhizopus oligosporus mycelial penetration and enzyme diffusion in soya bean tempee. Process Biochem. 1998; 33 (7): 741–747. Publisher Full Text 56. Vong WC, Hua XY, Liu SQ: Solis-state fermentation with Rhizopus oligosporus and Yarrowia lipolytia improved nutritional and flavor properties of okara. LWT. 2018; 90 : 316–322. Publisher Full Text 57. Jannathulla R, Dayal JS, Ambasankar K, et al. : Effect of Aspergillus niger fermented soybean meal and sunflower oil cake on growth carcass composition and hemolymph indices in Peneus vannamei Boone , 1931. Aquaculture. 2018; 486 : 1–8. Publisher Full Text 58. Huang L, Ran CHS, Ren P, et al. : Effects of dietary Saccharomyces cerevisae culture or live cells with Bacillus amyloliquefaciens spores on growth performance, gut mucosal morphology, hsp70 gene expression, and disease resistance of juvenile common carp ( Cyprinus carpio ). Aquaculture. 2015; 438 : 33–38. Publisher Full Text 59. Oliveira TS, Khan KU, Boarartt AZ, et al. : Evaluation of the optimum dietary essential amino acid pattern for adult pacu ( Piarachus mesopotamicus ). Aquaculture. 2021; 540 : 736686. Publisher Full Text 60. Prabu DL, Ebeneezar S, Chandrasekar S, et al. : Influence of graded level of dietary protein with equated level of limiting amino acids on growth, feed utilization, body indices and nutritive profile of snubnose pompano, Trachinotus blochii (Lacepede, 1801) reared in low saline water. Anim. Feed Sci. Technol. 2020; 269 : 114685. Publisher Full Text 61. Hua K, Bureau D: Estimating changes in essential amino acid requirements of rainbow trout and Atlantic salmon as a function of body weight or diet composition using a novel factorial requirement model. Aquaculture. 2019; 513 : 734440. Publisher Full Text 62. Lu X, Peng D, Chen X, et al. : Effect of dietary protein levels on growth, muscle composition, digestive enzymes activities, hemolymph biochemical indices and ovary development of pre-adult red swamp crayfish ( Procombarus clarkia ). Aqua. Rep. 2020; 18 : 100542. Publisher Full Text 63. Fatima S, Komal W, Manzoor F, et al. : Analysis of the growth performance, stress, profile of fatty acids and amino acids and cortisol in Tilapia ( Oreochromis niloticus ), cultured at high stocking density using in-pond raceway system. Saudi J. Biol. Sci. 2021; 28 (12): 7422–7431. PubMed Abstract | Publisher Full Text | Free Full Text 64. Syed R, Masood Z, Hasan HU, et al. : Growth performance, heamatological assessment and chemical composition of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) fed different levels of Aloe vera extract as feed additives in a closed aquaculture system. Saudi J. Biol. Sci. 2022; 29 : 296–303. PubMed Abstract | Publisher Full Text | Free Full Text 65. Besson M, Vandeputte M, van Arendonk JAM , et al. : Influence of water temperature on the economics of growth in fish farming: The case of sea bass ( Dicentrarchus labrax ) cage farming in the Mediterranean. Aquaculture. 2016; 462 : 47–55. Publisher Full Text 66. Besson M, Aubin J, Komen H, et al. : Environmental impacts of genetic improvement of growth rate and feed conversion ratio in fish farming under rearing density and nitrogen output limitations. J. Clean. Prod. 2015; 116 (10): 100–109. 67. Jobling M: The thermal growth coefficient (TGC) model of fish growth: A cautionary note. Short Communication. Aquac. Res. 2003; 34 : 581–584. Publisher Full Text 68. Zhou X, Wang J, Huang L, et al. : Modelling and controlling dissolved oxygen in recirculating aquaculture systems based on mechanism analysis and an adaptive PID controller. Comput. Electron. Agric. 2022; 192 : 106583. Publisher Full Text 69. Sun M, Hassan SG, Li D: Models for estimating feed intake in aquaculture: A review. Comput. Electron. Agric. 2016; 127 : 425–438. Publisher Full Text 70. Arriaga-Hernandez D, Hernandez C, Martinez-Montano E, et al. : Fish meal replacement by soybean products in aquaculture feeds for white snook, Centropomus viridis : Effect on growth, diet digestibility, and digestive capacity. Aquaculture. 2021; 530 : 735823. Publisher Full Text 71. Hassan HU, Ali QM, Ahmad N, et al. : Assessment of growth characteristics, the survival rate and body composition of Asian sea Lates calcarifer (Bloch, 1790) under different feeding rates in closed aquaculture system. Saudi J. Biol. Sci. 2021; 28 : 1324–1330. PubMed Abstract | Publisher Full Text | Free Full Text 72. Barbosa MC, Jatoba A, Vieira FN, et al. : Cultivation of Juvenile Fat Snook ( Centropomus parallelus , Poey, 1860) Fed Probiotic in Laboratory Conditions. Braz. Arch. Biol. Technol. 2011; 54 (4): 795–801. Publisher Full Text 73. Mohammadi M, Imani A, Farhangi M, et al. : Replacement of fishmeal with processed canola meal in diets for juvenile Nile tilapia ( Oreochromis niloticus ): Growth performance, mucosal innate immunity, hepatic oxidative status, liver and intestine histology. Aquaculture. 2019; 518 : 734824. Publisher Full Text 74. Siddik MAB, Foysal MJ, Fotedar R, et al. : Probiotic yeast Saccharomycs cerevisiae coupled with Lactobacillus casei modulates physiological performance and promotes gut microbiota in juvenile barramundi. Lates calcarifer. Aquaculture. 2022; 546 : 737346. Publisher Full Text 75. Anwar A, Wan AH, Omar S, et al. : The potential of a solid-state fermentation supplement to augment white lupin ( Lupinus albuas ) meal incorporation in diets for farmed common carp ( Cyprinus carpio ). Aqua. Rep. 2020; 17 : 100348. Publisher Full Text 76. Azrita A, Syandri H, Aryani N, et al. : Underlying data for ‘Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles’. At Figshare. 2022. Publisher Full Text Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 07 Feb 2023 ADD YOUR COMMENT Comment Author details Author details 1 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, West Sumatera, 25113, Indonesia 2 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Riau, Pekanbaru, 28293, Indonesia 3 Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Nahdlatul Ulama, Padang, West Sumatera, 25118, Indonesia Azrita Undefined Roles: Conceptualization, Data Curation, Formal Analysis, Methodology, Validation, Writing – Original Draft Preparation Hafrijal Syandri Roles: Data Curation, Funding Acquisition, Investigation, Methodology, Validation, Writing – Review & Editing Netti Aryani Roles: Data Curation, Formal Analysis, Methodology, Writing – Original Draft Preparation Ainul Mardiah Roles: Data Curation, Software, Writing – Original Draft Preparation Competing interests No competing interests were disclosed. Grant information We are grateful to the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia for funding this research under grant number: 076/E5/PG.02.00. PT/2022 The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Article Versions (2) version 2 Revised Published: 11 Sep 2023, 12:140 https://doi.org/10.12688/f1000research.124706.2 version 1 Published: 07 Feb 2023, 12:140 https://doi.org/10.12688/f1000research.124706.1 Copyright © 2023 Undefined A et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Download Export To Sciwheel Bibtex EndNote ProCite Ref. Manager (RIS) Sente metrics Views Downloads F1000Research - - PubMed Central info_outline Data from PMC are received and updated monthly. - - Citations open_in_new 0 open_in_new 0 open_in_new SEE MORE DETAILS CITE how to cite this article Undefined A, Syandri H, Aryani N and Mardiah A. Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.12688/f1000research.124706.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS track receive updates on this article Track an article to receive email alerts on any updates to this article. TRACK THIS ARTICLE Share Open Peer Review Current Reviewer Status: ? Key to Reviewer Statuses VIEW HIDE Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Version 2 VERSION 2 PUBLISHED 11 Sep 2023 Revised Views 0 Cite How to cite this report: Hashim F and Harun NO. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.155597.r205339 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v2#referee-response-205339 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 09 Oct 2023 Fatimah Hashim , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia Approved VIEWS 0 https://doi.org/10.5256/f1000research.155597.r205339 ... Continue reading READ ALL Approved Competing Interests: No competing interests were disclosed. Reviewer Expertise: Cytotoxicity, microscopy techniques, protozoology We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Hashim F and Harun NO. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.155597.r205339 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v2#referee-response-205339 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Hasani Q. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.155597.r205340 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v2#referee-response-205340 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 21 Sep 2023 Qadar Hasani , Program Studi Sumberdaya Akuatik, Fakultas Pertanian, Lampung University, Bandar Lampung, Lampung, Indonesia Approved VIEWS 0 https://doi.org/10.5256/f1000research.155597.r205340 The article has been revised properly. ... Continue reading READ ALL The article has been revised properly. I approve this article for indexing. Competing Interests: No competing interests were disclosed. Reviewer Expertise: Plant Botany and Ecology, Pollution, and Environmental Chemistry, The Dynamics of the Plankton Community, fish biology, and fish nutrition. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Hasani Q. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.155597.r205340 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v2#referee-response-205340 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Version 1 VERSION 1 PUBLISHED 07 Feb 2023 Views 0 Cite How to cite this report: Hasani Q. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.136930.r196596 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v1#referee-response-196596 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 25 Aug 2023 Qadar Hasani , Program Studi Sumberdaya Akuatik, Fakultas Pertanian, Lampung University, Bandar Lampung, Lampung, Indonesia Approved VIEWS 0 https://doi.org/10.5256/f1000research.136930.r196596 Abstract Background : Please add the sentence "of juvenile giant gourami" to the end of the sentence. Methods: Scientific names of Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae "please write in italic" ... Continue reading READ ALL Abstract Background : Please add the sentence "of juvenile giant gourami" to the end of the sentence. Methods: Scientific names of Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae "please write in italic" Results: Please add include the regression values (r 2 ) for the condition factor, feed intake, and protein efficiency ratio. Conclusion: Please add a sentence for the condition factor and body indices to the end of the sentence. Introduction Please include the scientific name of the Azolla plant. Please maintain consistency with the term "whole-body carcass". Please enhance the third paragraph by introducing or used fermented mushrooms (specifically Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae ). Please incorporate into the seventh paragraph the second objective of the research, which focuses on analyzing the condition factor and feed intake of giant gourami juveniles, along with their correlation to thermal growth coefficient, body index, and gut micromorphology. Methods: Ethical approval part; Please explain, namely "Gurami sago" whether it is a new species or strain. Experimental procedures part and sampling; Please ensure consistency with the term "gurami sago." or changed to "giant gourami" Please add state the manufacturing country of the light microscope (Olympus IX71) equipped with Image-Pro Plus 7.0 software. Results: The authors have displayed data concerning intestinal micromorphology (Table 4). It is recommended that the authors present all morphological findings related to intestinal micromorphology in the form of images/figures. Discussion: Please be consistent with the name used for example giant gourami. Conclusions: Please include regression values for each parameter, such as the condition factor (r 2 = 0.77) and protein efficiency ratio (r 2 = 0.74). Please maintain consistency with the term "whole-body carcass". Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Partly Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: Plant Botany and Ecology, Pollution, and Environmental Chemistry, The Dynamics of the Plankton Community, fish biology, and fish nutrition. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Hasani Q. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.136930.r196596 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v1#referee-response-196596 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 29 Aug 2023 Azrita Azrita , Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, 25113, Indonesia 29 Aug 2023 Author Response Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence ... Continue reading Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence "of juvenile giant gourami" at the end of the sentence. These suggestions will be fixed in a new revised version. Methods: We have agreed that some scientific names should be italicized as shown in the abstract, for example Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. These suggestions will be fixed in a new revised version. Results: We will add the regression value (r 2 ) for each parameter if the abstract does not exceed 300 words. Conclusion: We will add the condition factor and body indices if the abstract does not exceed 300 words. Introduction We will add scientific name of Azolla plant (Azolla pinnata) in new revised version. In the new revised version, we will consistently use the phrase "whole-body carcass" We have mutually decided to include the scientific names of three specific species: Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae, in the upcoming revised version. The seventh paragraph of the new revised version will encompass the second research objective. This objective entails investigating the condition factor concerning the feeding of giant gourami fingerlings and its correlation with the thermal growth coefficient, body index, and gut micromorphology. Methods Ethical Approval Section: The Sago gourami represents one of the giant gourami strains, and we will include it in the new revised version. We will consistently write "Giant gourami" in the revised version of the article. We will append a newly revised version of the article with the information that Japan is the nation where light microscopes (Olympus IX71) equipped with Image-Pro Plus 7.0 software are manufactured. In order to enhance the data presented in Table 4, we will incorporate images or figures depicting intestinal micromorphology within the new version of the revision. Discussion In the revised version, we will consistently use the name "Giant gourami". Conclusions: In the new revised version, we agreed to include the coefficient of determination (r 2 ) in the conclusion section, for the condition factor (r 2 = 0.77) and the protein efficiency ratio (r 2 = 0.74). Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence "of juvenile giant gourami" at the end of the sentence. These suggestions will be fixed in a new revised version. Methods: We have agreed that some scientific names should be italicized as shown in the abstract, for example Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. These suggestions will be fixed in a new revised version. Results: We will add the regression value (r 2 ) for each parameter if the abstract does not exceed 300 words. Conclusion: We will add the condition factor and body indices if the abstract does not exceed 300 words. Introduction We will add scientific name of Azolla plant (Azolla pinnata) in new revised version. In the new revised version, we will consistently use the phrase "whole-body carcass" We have mutually decided to include the scientific names of three specific species: Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae, in the upcoming revised version. The seventh paragraph of the new revised version will encompass the second research objective. This objective entails investigating the condition factor concerning the feeding of giant gourami fingerlings and its correlation with the thermal growth coefficient, body index, and gut micromorphology. Methods Ethical Approval Section: The Sago gourami represents one of the giant gourami strains, and we will include it in the new revised version. We will consistently write "Giant gourami" in the revised version of the article. We will append a newly revised version of the article with the information that Japan is the nation where light microscopes (Olympus IX71) equipped with Image-Pro Plus 7.0 software are manufactured. In order to enhance the data presented in Table 4, we will incorporate images or figures depicting intestinal micromorphology within the new version of the revision. Discussion In the revised version, we will consistently use the name "Giant gourami". Conclusions: In the new revised version, we agreed to include the coefficient of determination (r 2 ) in the conclusion section, for the condition factor (r 2 = 0.77) and the protein efficiency ratio (r 2 = 0.74). Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 29 Aug 2023 Azrita Azrita , Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, 25113, Indonesia 29 Aug 2023 Author Response Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence ... Continue reading Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence "of juvenile giant gourami" at the end of the sentence. These suggestions will be fixed in a new revised version. Methods: We have agreed that some scientific names should be italicized as shown in the abstract, for example Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. These suggestions will be fixed in a new revised version. Results: We will add the regression value (r 2 ) for each parameter if the abstract does not exceed 300 words. Conclusion: We will add the condition factor and body indices if the abstract does not exceed 300 words. Introduction We will add scientific name of Azolla plant (Azolla pinnata) in new revised version. In the new revised version, we will consistently use the phrase "whole-body carcass" We have mutually decided to include the scientific names of three specific species: Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae, in the upcoming revised version. The seventh paragraph of the new revised version will encompass the second research objective. This objective entails investigating the condition factor concerning the feeding of giant gourami fingerlings and its correlation with the thermal growth coefficient, body index, and gut micromorphology. Methods Ethical Approval Section: The Sago gourami represents one of the giant gourami strains, and we will include it in the new revised version. We will consistently write "Giant gourami" in the revised version of the article. We will append a newly revised version of the article with the information that Japan is the nation where light microscopes (Olympus IX71) equipped with Image-Pro Plus 7.0 software are manufactured. In order to enhance the data presented in Table 4, we will incorporate images or figures depicting intestinal micromorphology within the new version of the revision. Discussion In the revised version, we will consistently use the name "Giant gourami". Conclusions: In the new revised version, we agreed to include the coefficient of determination (r 2 ) in the conclusion section, for the condition factor (r 2 = 0.77) and the protein efficiency ratio (r 2 = 0.74). Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence "of juvenile giant gourami" at the end of the sentence. These suggestions will be fixed in a new revised version. Methods: We have agreed that some scientific names should be italicized as shown in the abstract, for example Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. These suggestions will be fixed in a new revised version. Results: We will add the regression value (r 2 ) for each parameter if the abstract does not exceed 300 words. Conclusion: We will add the condition factor and body indices if the abstract does not exceed 300 words. Introduction We will add scientific name of Azolla plant (Azolla pinnata) in new revised version. In the new revised version, we will consistently use the phrase "whole-body carcass" We have mutually decided to include the scientific names of three specific species: Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae, in the upcoming revised version. The seventh paragraph of the new revised version will encompass the second research objective. This objective entails investigating the condition factor concerning the feeding of giant gourami fingerlings and its correlation with the thermal growth coefficient, body index, and gut micromorphology. Methods Ethical Approval Section: The Sago gourami represents one of the giant gourami strains, and we will include it in the new revised version. We will consistently write "Giant gourami" in the revised version of the article. We will append a newly revised version of the article with the information that Japan is the nation where light microscopes (Olympus IX71) equipped with Image-Pro Plus 7.0 software are manufactured. In order to enhance the data presented in Table 4, we will incorporate images or figures depicting intestinal micromorphology within the new version of the revision. Discussion In the revised version, we will consistently use the name "Giant gourami". Conclusions: In the new revised version, we agreed to include the coefficient of determination (r 2 ) in the conclusion section, for the condition factor (r 2 = 0.77) and the protein efficiency ratio (r 2 = 0.74). Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Hashim F and Harun NO. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.136930.r163500 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v1#referee-response-163500 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 28 Feb 2023 Fatimah Hashim , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia Approved with Reservations VIEWS 0 https://doi.org/10.5256/f1000research.136930.r163500 Dear authors, thank you for your paper and we appreciate the effort and contribution that you have made to the field. However, we noticed that your paper lacks results for the micromorphology part, which makes it difficult ... Continue reading READ ALL Dear authors, thank you for your paper and we appreciate the effort and contribution that you have made to the field. However, we noticed that your paper lacks results for the micromorphology part, which makes it difficult to fully understand the conclusions of your study. Specifically, we're unable to fully assess the morphological characteristics of the samples and how they relate to the overall findings. Therefore, we suggest that you consider adding some additional data/results where necessary. Additionally: Some scientific names were not italicised. The number at the beginning of the sentence must be spelt out. Some literature in the introduction compares the use of coconut water on terrestrial animal or human levels. It is better to compare with aquatic organisms that are more related to the gourami. The author should provide all morphology results on gut micromorphology (in the form of images/ figures), not only the data measurement (Table 4.) Additional comments from Fatimah Hashim attached here . Additional comments from Nor Omaima Harun attached here . Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Partly Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: Cytotoxicity, microscopy techniques, protozoology We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however we have significant reservations, as outlined above. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Hashim F and Harun NO. Reviewer Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.136930.r163500 ) The direct URL for this report is: https://f1000research.com/articles/12-140/v1#referee-response-163500 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 11 Sep 2023 Azrita Azrita , Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, 25113, Indonesia 11 Sep 2023 Author Response Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be ... Continue reading Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be italicized as in the Abstract : Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. Author response: We agree that when a number appears at the beginning of a sentence, the number must be spelled out with letters, just like the example in the Abstract where the number 100 is changed to One hundred, the number 2 liters is changed to Two litres, and 1 litres changed to One litres. Author's Response: Previously, we stated in the introduction that animals or humans have used coconut water on land. However, no literature has been found testing the use of coconut water on aquatic animals, including gourami. Therefore, this study focuses on the novelty of using new product formulas for coconut water, palm sugar, and mushroom in aquatic animals, especially giant gourami. Author response: Olympus Model IX71, HBO 100W, Phase Contrast Microscopy, Fluorite Lenses, Inverted Cell Culture, Fluorescence Microscope, Country: Japan. Author response: We have prepared gut micromorphology images and are willing to display Figure 4 in the article if the editorial team approves the addition (Please open this link ). Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be italicized as in the Abstract : Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. Author response: We agree that when a number appears at the beginning of a sentence, the number must be spelled out with letters, just like the example in the Abstract where the number 100 is changed to One hundred, the number 2 liters is changed to Two litres, and 1 litres changed to One litres. Author's Response: Previously, we stated in the introduction that animals or humans have used coconut water on land. However, no literature has been found testing the use of coconut water on aquatic animals, including gourami. Therefore, this study focuses on the novelty of using new product formulas for coconut water, palm sugar, and mushroom in aquatic animals, especially giant gourami. Author response: Olympus Model IX71, HBO 100W, Phase Contrast Microscopy, Fluorite Lenses, Inverted Cell Culture, Fluorescence Microscope, Country: Japan. Author response: We have prepared gut micromorphology images and are willing to display Figure 4 in the article if the editorial team approves the addition (Please open this link ). Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 11 Sep 2023 Azrita Azrita , Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, 25113, Indonesia 11 Sep 2023 Author Response Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be ... Continue reading Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be italicized as in the Abstract : Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. Author response: We agree that when a number appears at the beginning of a sentence, the number must be spelled out with letters, just like the example in the Abstract where the number 100 is changed to One hundred, the number 2 liters is changed to Two litres, and 1 litres changed to One litres. Author's Response: Previously, we stated in the introduction that animals or humans have used coconut water on land. However, no literature has been found testing the use of coconut water on aquatic animals, including gourami. Therefore, this study focuses on the novelty of using new product formulas for coconut water, palm sugar, and mushroom in aquatic animals, especially giant gourami. Author response: Olympus Model IX71, HBO 100W, Phase Contrast Microscopy, Fluorite Lenses, Inverted Cell Culture, Fluorescence Microscope, Country: Japan. Author response: We have prepared gut micromorphology images and are willing to display Figure 4 in the article if the editorial team approves the addition (Please open this link ). Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be italicized as in the Abstract : Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. Author response: We agree that when a number appears at the beginning of a sentence, the number must be spelled out with letters, just like the example in the Abstract where the number 100 is changed to One hundred, the number 2 liters is changed to Two litres, and 1 litres changed to One litres. Author's Response: Previously, we stated in the introduction that animals or humans have used coconut water on land. However, no literature has been found testing the use of coconut water on aquatic animals, including gourami. Therefore, this study focuses on the novelty of using new product formulas for coconut water, palm sugar, and mushroom in aquatic animals, especially giant gourami. Author response: Olympus Model IX71, HBO 100W, Phase Contrast Microscopy, Fluorite Lenses, Inverted Cell Culture, Fluorescence Microscope, Country: Japan. Author response: We have prepared gut micromorphology images and are willing to display Figure 4 in the article if the editorial team approves the addition (Please open this link ). Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 07 Feb 2023 ADD YOUR COMMENT Comment keyboard_arrow_left keyboard_arrow_right Open Peer Review Reviewer Status info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Reviewer Reports Invited Reviewers 1 2 Version 2 (revision) 11 Sep 23 read read Version 1 07 Feb 23 read read Fatimah Hashim , Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun , Universiti Malaysia Terengganu, Terengganu, Malaysia Qadar Hasani , Lampung University, Bandar Lampung, Indonesia Comments on this article All Comments (0) Add a comment Sign up for content alerts Sign Up You are now signed up to receive this alert Browse by related subjects keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2023 Hashim F et al. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 09 Oct 2023 | for Version 2 Fatimah Hashim , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia 0 Views copyright © 2023 Hashim F et al. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Approved Competing Interests No competing interests were disclosed. Reviewer Expertise Cytotoxicity, microscopy techniques, protozoology We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Hashim F and Harun NO. Peer Review Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.155597.r205339) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/12-140/v2#referee-response-205339 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2023 Hasani Q. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 21 Sep 2023 | for Version 2 Qadar Hasani , Program Studi Sumberdaya Akuatik, Fakultas Pertanian, Lampung University, Bandar Lampung, Lampung, Indonesia 0 Views copyright © 2023 Hasani Q. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions The article has been revised properly. I approve this article for indexing. Competing Interests No competing interests were disclosed. Reviewer Expertise Plant Botany and Ecology, Pollution, and Environmental Chemistry, The Dynamics of the Plankton Community, fish biology, and fish nutrition. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Hasani Q. Peer Review Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.155597.r205340) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/12-140/v2#referee-response-205340 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2023 Hasani Q. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 25 Aug 2023 | for Version 1 Qadar Hasani , Program Studi Sumberdaya Akuatik, Fakultas Pertanian, Lampung University, Bandar Lampung, Lampung, Indonesia 0 Views copyright © 2023 Hasani Q. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Abstract Background : Please add the sentence "of juvenile giant gourami" to the end of the sentence. Methods: Scientific names of Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae "please write in italic" Results: Please add include the regression values (r 2 ) for the condition factor, feed intake, and protein efficiency ratio. Conclusion: Please add a sentence for the condition factor and body indices to the end of the sentence. Introduction Please include the scientific name of the Azolla plant. Please maintain consistency with the term "whole-body carcass". Please enhance the third paragraph by introducing or used fermented mushrooms (specifically Aspergillus niger , Rhizopus oligosporus , and Saccharomyces cerevisiae ). Please incorporate into the seventh paragraph the second objective of the research, which focuses on analyzing the condition factor and feed intake of giant gourami juveniles, along with their correlation to thermal growth coefficient, body index, and gut micromorphology. Methods: Ethical approval part; Please explain, namely "Gurami sago" whether it is a new species or strain. Experimental procedures part and sampling; Please ensure consistency with the term "gurami sago." or changed to "giant gourami" Please add state the manufacturing country of the light microscope (Olympus IX71) equipped with Image-Pro Plus 7.0 software. Results: The authors have displayed data concerning intestinal micromorphology (Table 4). It is recommended that the authors present all morphological findings related to intestinal micromorphology in the form of images/figures. Discussion: Please be consistent with the name used for example giant gourami. Conclusions: Please include regression values for each parameter, such as the condition factor (r 2 = 0.77) and protein efficiency ratio (r 2 = 0.74). Please maintain consistency with the term "whole-body carcass". Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Partly Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise Plant Botany and Ecology, Pollution, and Environmental Chemistry, The Dynamics of the Plankton Community, fish biology, and fish nutrition. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (1) Author Response 29 Aug 2023 Azrita Azrita, Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, 25113, Indonesia Reviewer Report response by Qadar Hasani, Aquatic Resources Study Program, Faculty of Agriculture, Lampung University, Bandar Lampung, Lampung, Indonesia. Abstract Background: We agree to add the sentence "of juvenile giant gourami" at the end of the sentence. These suggestions will be fixed in a new revised version. Methods: We have agreed that some scientific names should be italicized as shown in the abstract, for example Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. These suggestions will be fixed in a new revised version. Results: We will add the regression value (r 2 ) for each parameter if the abstract does not exceed 300 words. Conclusion: We will add the condition factor and body indices if the abstract does not exceed 300 words. Introduction We will add scientific name of Azolla plant (Azolla pinnata) in new revised version. In the new revised version, we will consistently use the phrase "whole-body carcass" We have mutually decided to include the scientific names of three specific species: Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae, in the upcoming revised version. The seventh paragraph of the new revised version will encompass the second research objective. This objective entails investigating the condition factor concerning the feeding of giant gourami fingerlings and its correlation with the thermal growth coefficient, body index, and gut micromorphology. Methods Ethical Approval Section: The Sago gourami represents one of the giant gourami strains, and we will include it in the new revised version. We will consistently write "Giant gourami" in the revised version of the article. We will append a newly revised version of the article with the information that Japan is the nation where light microscopes (Olympus IX71) equipped with Image-Pro Plus 7.0 software are manufactured. In order to enhance the data presented in Table 4, we will incorporate images or figures depicting intestinal micromorphology within the new version of the revision. Discussion In the revised version, we will consistently use the name "Giant gourami". Conclusions: In the new revised version, we agreed to include the coefficient of determination (r 2 ) in the conclusion section, for the condition factor (r 2 = 0.77) and the protein efficiency ratio (r 2 = 0.74). View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Hasani Q. Peer Review Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.136930.r196596) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/12-140/v1#referee-response-196596 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2023 Hashim F et al. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 28 Feb 2023 | for Version 1 Fatimah Hashim , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun , BIOSES Research Interest Group, Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Terengganu, Malaysia 0 Views copyright © 2023 Hashim F et al. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved With Reservations info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Dear authors, thank you for your paper and we appreciate the effort and contribution that you have made to the field. However, we noticed that your paper lacks results for the micromorphology part, which makes it difficult to fully understand the conclusions of your study. Specifically, we're unable to fully assess the morphological characteristics of the samples and how they relate to the overall findings. Therefore, we suggest that you consider adding some additional data/results where necessary. Additionally: Some scientific names were not italicised. The number at the beginning of the sentence must be spelt out. Some literature in the introduction compares the use of coconut water on terrestrial animal or human levels. It is better to compare with aquatic organisms that are more related to the gourami. The author should provide all morphology results on gut micromorphology (in the form of images/ figures), not only the data measurement (Table 4.) Additional comments from Fatimah Hashim attached here . Additional comments from Nor Omaima Harun attached here . Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Partly Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise Cytotoxicity, microscopy techniques, protozoology We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however we have significant reservations, as outlined above. reply Respond to this report Responses (1) Author Response 11 Sep 2023 Azrita Azrita, Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, 25113, Indonesia Reviewer Report by Fatimah Hashim, Universiti Malaysia Terengganu, Terengganu, Malaysia Nor Omaima Harun, Universiti Malaysia Terengganu, Terengganu, Malaysia Author Respond: We agree that several scientific names should be italicized as in the Abstract : Aspergillus niger, Rhizopus oligosporus, and Saccharomyces cerevisiae. Author response: We agree that when a number appears at the beginning of a sentence, the number must be spelled out with letters, just like the example in the Abstract where the number 100 is changed to One hundred, the number 2 liters is changed to Two litres, and 1 litres changed to One litres. Author's Response: Previously, we stated in the introduction that animals or humans have used coconut water on land. However, no literature has been found testing the use of coconut water on aquatic animals, including gourami. Therefore, this study focuses on the novelty of using new product formulas for coconut water, palm sugar, and mushroom in aquatic animals, especially giant gourami. Author response: Olympus Model IX71, HBO 100W, Phase Contrast Microscopy, Fluorite Lenses, Inverted Cell Culture, Fluorescence Microscope, Country: Japan. Author response: We have prepared gut micromorphology images and are willing to display Figure 4 in the article if the editorial team approves the addition (Please open this link ). View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Hashim F and Harun NO. Peer Review Report For: Effect of feed enriched by products formulated from coconut water, palm sap sugar, and mushroom on the chemical composition of feed and carcass, growth performance, body indices, and gut micromorphology of giant gourami, Osphronemus goramy (Lacepède, 1801), juveniles [version 2; peer review: 2 approved] . F1000Research 2023, 12 :140 ( https://doi.org/10.5256/f1000research.136930.r163500) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/12-140/v1#referee-response-163500 Alongside their report, reviewers assign a status to the article: Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions Adjust parameters to alter display View on desktop for interactive features Includes Interactive Elements View on desktop for interactive features Competing Interests Policy Provide sufficient details of any financial or non-financial competing interests to enable users to assess whether your comments might lead a reasonable person to question your impartiality. 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