In situ Hi-C for mosquito embryos

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AI-generated summary by claude@2026-07+body, 2026-07-05

This paper describes a step-by-step protocol for performing in situ Hi-C experiments specifically on mosquito embryos.

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AI-generated deep summary by claude@2026-07, 2026-07-05 · read from full text

This protocols.io paper describes an in situ Hi-C workflow for mosquito embryos, detailing step-by-step preparation and processing to capture 3D chromatin interactions directly within developing tissue. It focuses on generating chromosome conformation data suitable for downstream analysis from embryos, rather than reporting specific biological findings from a hypothesis-driven study. The main limitation is that, as a protocol resource, it provides methodological instructions without including results that demonstrate performance across conditions or stages. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Abstract The step-by-step Hi-C protocol is described for mosquito embryos at the 15-18 h developmental stage. In brief, we collect mosquito eggs, fix them with 3% formaldehyde, and prepare Hi-C libraries using nuclei isolated from ~1000-3000 embryos of mixed sexes. We use MboI restriction enzyme and T4-ligase to generate two biological replicates per species. Libraries can be prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina. The protocol was adapted for Anopheles species and successfully verified for Culex and Aedes embryos.
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