Different effects of cortisol on pro-inflammatory gene expressions of LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells

Abstract Background: Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogen like E.coli . However, the mechanism underlying how cortisol regulates E.coli -induced inflammatory response in bovine endometrial epithelial cells (BEEC) remains elusive. Results: Cortisol decreased the expressions of IL1β, IL6, TNF-α, IL8, and TLR4 mRNA in BEEC treated with LPS or heat-killed E.coli , but up-regulated the these gene expressions in BEEC stimulated by live E.coli . Conclusion: Cortisol exerted the anti-inflammation action on LPS- or heat-killed E.coli -stimulated BEEC, but the pro-inflammation action on live E.coli -induced BEEC.