The Enzyme Function and Application Analysis of A Cellulose-degrading Bacterium RA Based on Genomic Analysis

The Enzyme Function and Application Analysis of A Cellulose-degrading Bacterium RA Based on Genomic Analysis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The Enzyme Function and Application Analysis of A Cellulose-degrading Bacterium RA Based on Genomic Analysis Yue Ren, Jie Zhang, Konglu Zhang, Chen Zhu, Juan Zhang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9195208/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study identifies the multi-enzyme system of a cellulose-degrading Bacillus safensis strain RA through screening and biological characterization. Genomic analysis revealed that the strain harbors both cellulase and pectinase genes. Optimal cellulase production (26.57 ± 0.46 U/mL) was achieved with 10 g/L CMC-Na, 4 g/L peptone, 2.0% (v/v) inoculum, 40°C, pH 7.7, and 3.25 days, while optimal pectinase activity (76.36 ± 2.23 U/mL) was obtained after 3 days at 40°C and pH 5. Functional studies showed that filter paper strips began to break within 5 hours of inoculation and fragmented into small pieces by 36 hours. In juice clarification, the optimal conditions for apple juice were 20% crude enzyme, 50°C, and 1 hour (27.69% clarification), and for orange juice, 30% crude enzyme, 40°C, and 3 hours (39.7% clarification). This strain shows potential as a compound biological agent for dual-enzyme applications. The study highlights the utility of genomic techniques in analyzing multifunctional enzymes, overcoming the “single-function orientation” in genomic research and providing insights for low-cost industrial enzyme production. Bacillus safensis Whole-genome sequencing Response surface optimization Degradation of filter paper strips Pectinase juice clarification Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9195208","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":611033695,"identity":"5f8f82d5-84ca-4a65-98c7-a4f7a7509037","order_by":0,"name":"Yue Ren","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Yue","middleName":"","lastName":"Ren","suffix":""},{"id":611033698,"identity":"3ddb295d-8cd5-4c6b-bacc-2c247787c3b2","order_by":1,"name":"Jie Zhang","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Jie","middleName":"","lastName":"Zhang","suffix":""},{"id":611033699,"identity":"da5b6cef-26b5-41e5-b755-8400d5e5b0b0","order_by":2,"name":"Konglu Zhang","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Konglu","middleName":"","lastName":"Zhang","suffix":""},{"id":611033700,"identity":"e37b72f5-046c-4461-adb8-a6948ff42378","order_by":3,"name":"Chen Zhu","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Chen","middleName":"","lastName":"Zhu","suffix":""},{"id":611033701,"identity":"f46b8696-67e4-41f4-abc6-05ebfb955d21","order_by":4,"name":"Juan Zhang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA0ElEQVRIiWNgGAWjYDACCYaEAx8qJHjY2BsbH34gUkviwRlnLOT4eQ43G0sQqYX5MG9bhbHkjPQ2AR5idMjPbnhwmOeMROKGmw/bgPrt5HQbCGhhnHMg4eCcCqCW24ltDwoYko3NDhDQwiyRkHDgzRmwlnYDCYYDidsIaWEDaeFtAznsYJsEDzFaeIBaDgK1AL3PSKQWCZCWGWckgIGcCAxkAyL8Ij8jJ/nDh4o6YFQef/jwQ4WdHEEtQKclIHEMCCoHAXbCpo6CUTAKRsEIBwAo6kjouDVm4AAAAABJRU5ErkJggg==","orcid":"","institution":"","correspondingAuthor":true,"prefix":"","firstName":"Juan","middleName":"","lastName":"Zhang","suffix":""}],"badges":[],"createdAt":"2026-03-23 03:53:37","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9195208/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9195208/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":106960687,"identity":"370a9d70-e20a-412c-bb9f-975b94eb2b17","added_by":"auto","created_at":"2026-04-15 09:22:38","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1215900,"visible":true,"origin":"","legend":"","description":"","filename":"TheEnzymeFunctionandApplicationAnalysisofACellulosedegradingBacteriumRABasedonGenomicAnalysis.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9195208/v1_covered_686888b1-3cce-468e-ae1c-041c6fe8fa68.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"The Enzyme Function and Application Analysis of A Cellulose-degrading Bacterium RA Based on Genomic Analysis","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Bacillus safensis, Whole-genome sequencing, Response surface optimization, Degradation of filter paper strips, Pectinase juice clarification","lastPublishedDoi":"10.21203/rs.3.rs-9195208/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9195208/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study identifies the multi-enzyme system of a cellulose-degrading Bacillus safensis strain RA through screening and biological characterization. Genomic analysis revealed that the strain harbors both cellulase and pectinase genes. Optimal cellulase production (26.57\u0026thinsp;\u0026plusmn;\u0026thinsp;0.46 U/mL) was achieved with 10 g/L CMC-Na, 4 g/L peptone, 2.0% (v/v) inoculum, 40\u0026deg;C, pH 7.7, and 3.25 days, while optimal pectinase activity (76.36\u0026thinsp;\u0026plusmn;\u0026thinsp;2.23 U/mL) was obtained after 3 days at 40\u0026deg;C and pH 5. Functional studies showed that filter paper strips began to break within 5 hours of inoculation and fragmented into small pieces by 36 hours. In juice clarification, the optimal conditions for apple juice were 20% crude enzyme, 50\u0026deg;C, and 1 hour (27.69% clarification), and for orange juice, 30% crude enzyme, 40\u0026deg;C, and 3 hours (39.7% clarification). This strain shows potential as a compound biological agent for dual-enzyme applications. 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