Hyphomonas sediminis sp. nov., isolated from marine sediment

A Gram-staining-negative, aerobic and pear-shaped bacterial strain, designated WL0036 T , was isolated from coastal sediment sample collected in Nantong city, Jiangsu province of China (120°51′13″E, 32°6′26″N) in October 2020. Strain WL0036 T was found to grow at 20–37°C (optimum, 28°C) with 0–9.0% NaCl (optimum, 2.5-4.0%) and displayed alkaliphilic growth with the pH range of pH 6.0–10.0 (optimum, pH 7.0–8.0). The polar lipids profile of strain WL0036 T included phosphatidylcholine, phosphatidylethanolamine, glycolipid and an unidentified lipid. The major isoprenoid quinone was determined to be Q-11 and the major fatty acids were C 16:0 , 11-methyl-C 18:1 ω 7 c , and summed features 8 (C 18:1 ω 6 c and/or C 18:1 ω 7 c ). The G + C content of genomic DNA was 61.8%. Phylogenetic trees constructed based on 16S rRNA gene sequence and bac120 gene set indicted that strain WL0036 T clustered with strains Hyphomonas neptunium ATCC 15444 T and H. polymorpha PS728 T . The average nucleotide identities between strain WL0036 T and strains H. neptunium ATCC 15444 T and H. polymorpha PS728 T were 80.7% and 81.2%, respectively. Strain WL0036 T showed 22.8% and 23.2% of digital DNA-DNA hybridization identities with H. neptunium ATCC 15444 T and H. polymorpha PS728 T , respectively. As inferred from the phenotypic and genotypic characteristics and the phylogenetic trees, strain WL0036 T ought to be recognized as a novel species in genus Hyphomonas , for which the name Hyphomonas sediminis sp. nov. is proposed. The type strain is WL0036 T (= MCCC 1K05843 T  = JCM 34658 T  = GDMCC 1.2413 T ).