Green synthesis of silver nanoparticles loaded in bacterial cellulose obtained from mothers of vinegar: In situ photoreduction, characterization, and antibacterial activity

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Abstract Silver nanoparticles (AgNPs) are known for their outstanding antimicrobial activity. However, most traditional methods used to synthesize silver nanoparticles require either the use of chemical reducing agents and activators that are highly polluting or that require investments in expensive equipment with high energy consumption. Thus, finding green, fast, and simple alternatives to synthesize silver particles for antiseptic purposes is important. In this study, AgNPs were synthesized in situ on a matrix of bacterial cellulose (BC) obtained from mothers of pineapple vinegar by a photoreduction reaction assisted by a sodium lamp that emitted yellow light (λ = 589 nm). BC-AgNP films were synthesized with AgNP concentrations of 5, 25, 50, 75, and 100 ppm, and their antibacterial activity was evaluated; the results revealed that they inhibited the growth of Staphylococcus aureus and Escherichia coli by up to 100% and 95.6%, respectively.
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Green synthesis of silver nanoparticles loaded in bacterial cellulose obtained from mothers of vinegar: In situ photoreduction, characterization, and antibacterial activity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Green synthesis of silver nanoparticles loaded in bacterial cellulose obtained from mothers of vinegar: In situ photoreduction, characterization, and antibacterial activity Ana Beatriz Morales Cepeda, Abigail María Díaz-Guerrero, Karla Sintia Gallardo Urguell, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8882830/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Silver nanoparticles (AgNPs) are known for their outstanding antimicrobial activity. However, most traditional methods used to synthesize silver nanoparticles require either the use of chemical reducing agents and activators that are highly polluting or that require investments in expensive equipment with high energy consumption. Thus, finding green, fast, and simple alternatives to synthesize silver particles for antiseptic purposes is important. In this study, AgNPs were synthesized in situ on a matrix of bacterial cellulose (BC) obtained from mothers of pineapple vinegar by a photoreduction reaction assisted by a sodium lamp that emitted yellow light (λ = 589 nm). BC-AgNP films were synthesized with AgNP concentrations of 5, 25, 50, 75, and 100 ppm, and their antibacterial activity was evaluated; the results revealed that they inhibited the growth of Staphylococcus aureus and Escherichia coli by up to 100% and 95.6%, respectively. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8882830","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":602624046,"identity":"41ef0b45-cf94-4a0c-b68d-54d18ba6ff3a","order_by":0,"name":"Ana Beatriz Morales 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