Experimental study on inhibiting effect of recombinant human endostatin on ectopic endometrium in rats with endometriosis

Objective: To establish rat model of endometriosis treated with endostatin,explore the curative effect of endostatin.Methods: SD rat model of endometriosis was established by autologous transplantation method,and then the rats were randomly divided into model group,danazol group,endostatin group and sham operation group( 8 rats). The rats were treated with different drugs at four weeks after operation,then the rats in four groups were killed at 14 days after drug administration,electron microscope was used to observe ultrastructure,immunohistochemical method was used to detect the expressions of vascular endothelial growth factor( VEGF) and CD34. Results: Rat model was established successfully in 32 rats among 37 rats; at 14 days after drug administration,the cell ultrastructure changed under electron microscope in danazol group and endostatin group,including irregular cell nucleus and nuclear membrane notch,fuzzy mitochondrial cristae,autophagosome in cytoplasm. The structure of capsule inner wall of endometrial epithelial cells in model group was normal,and the structure of eutopic endometrial in sham operation group was normal. Compared with sham operation group,the expression levels of VEGF and CD34( microvessel density) were higher in model group,there were statistically significant differences( P 0. 05). The expression levels of VEGF and CD34( microvessel density) in danazol group and endostatin group were statistically significantly lower than those in model group( P 0. 05). There was no statistically significant difference between danazol group and endostatin group( P 0. 05).Conclusion: The successful rate of SD rat model establishment of endometriosis is high,endostatin can inhibit the growth of ectopic endometrium.