Finding Consensus miRNAs Silencing KLF1 Expression as A Promising Therapeutic Option of Sickle Cell Anemia

1 Sickle cell anemia (SCA) is the almost the severest hemoglobinopathy known with no cure till date. Patients with SCA has a shorter lifespan and suffer from painful crises and end-organ damages. The goal of this in silico work is to find the consensus miRNAs targeting KLF1 gene, responsible for HbF-to-HbA switching followed by generating the corresponding miRNA sponge for gene silencing purposes. 3 publicly available databases were searched, miRDB, miRWalk and TargetScan. Afterwards, the hybridization examination of the predicted miRNAs was evaluated. Finally, the design of miRNA sponge as a means to target miRNA was performed. In conclusion, hsa-miR-330-5p was the best miRNA targeting KLF1 gene in many aspects and its miRNA sponge sequence was provided.