Fast fabrication of “all-in-one” injectable hydrogels as antibiotic alternatives for enhanced bacterial inhibition and accelerating wound healing

Fast fabrication of “all-in-one” injectable hydrogels as antibiotic alternatives for enhanced bacterial inhibition and accelerating wound healing | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Fast fabrication of “all-in-one” injectable hydrogels as antibiotic alternatives for enhanced bacterial inhibition and accelerating wound healing Juan Xin, Zhangyou Yang, Shurong Zhang, Lili Sun, Xin Wang, Yang Tang, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3999983/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 26 Jul, 2024 Read the published version in Journal of Nanobiotechnology → Version 1 posted 9 You are reading this latest preprint version Abstract Skin wound infection has become a notable medical threat. Herein, the polysaccharide-based injectable hydrogels with multifunctionality were developed by a simple and fast gelation process not only to inactivate bacteria but also to accelerate bacteria-infected wound healing. Sodium nitroprusside (SNP) loaded PCN-224 metal-organic frameworks nanoparticles were introduced into the polymer matrix formed by the dynamic and reversible coordinate bonds between Ag + with carboxyl and amino or hydroxyl groups on carboxymethyl chitosan (CMCS), multiple hydrogen bonds and electrostatic interactions in the polymer to fabricate SNP@PCN@Gel hydrogels. SNP@PCN@Gel displayed interconnected porous structure, excellent self-healing capacity, low cytotoxicity, good blood compatibility, and robust antibacterial activity. SNP@PCN@Gel could produce reactive oxygen species (ROS), showed rapid generation of NO along with Fe 2+ and long-term sustained release of Ag + , thereby effectively killing bacteria by synergistic photothermal (hyperthermia), photodynamic (ROS), chemodynamic (Fenton reaction), gas (NO) and ion (Ag + and -NH 3 + in CMCS) therapy. Remarkably, the hydrogels significantly promoted granulation tissue formation, reepithelization, collagen deposition and angiogenesis as well as wound contraction in bacteria-infected wound healing. Taken together, the strategy represented a general method to engineer the unprecedented photoactivatable “all-in-one” hydrogels with enhanced antibacterial activity and paved a new way for development of antibiotic alternatives and wound dressing. Metal-organic framework Carboxymethyl chitosan Hydrogels Nitric oxide Antibiotic alternatives Wound healing Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementarymaterialsforNB.docx Cite Share Download PDF Status: Published Journal Publication published 26 Jul, 2024 Read the published version in Journal of Nanobiotechnology → Version 1 posted Editorial decision: Revision requested 15 Apr, 2024 Reviews received at journal 12 Apr, 2024 Reviews received at journal 10 Apr, 2024 Reviewers agreed at journal 05 Apr, 2024 Reviewers agreed at journal 05 Apr, 2024 Reviewers invited by journal 05 Apr, 2024 Editor assigned by journal 05 Mar, 2024 Submission checks completed at journal 05 Mar, 2024 First submitted to journal 29 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3999983","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":276501296,"identity":"9574b740-6a53-4cd9-9a87-4cf287444ea3","order_by":0,"name":"Juan Xin","email":"","orcid":"","institution":"Chongqing Medical University","correspondingAuthor":false,"prefix":"","firstName":"Juan","middleName":"","lastName":"Xin","suffix":""},{"id":276501297,"identity":"a0dce08e-799f-4c2d-9045-15dfecdd05dc","order_by":1,"name":"Zhangyou Yang","email":"","orcid":"","institution":"Chongqing Medical 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Herein, the polysaccharide-based injectable hydrogels with multifunctionality were developed by a simple and fast gelation process not only to inactivate bacteria but also to accelerate bacteria-infected wound healing. Sodium nitroprusside (SNP) loaded PCN-224 metal-organic frameworks nanoparticles were introduced into the polymer matrix formed by the dynamic and reversible coordinate bonds between Ag\u003csup\u003e+\u003c/sup\u003e with carboxyl and amino or hydroxyl groups on carboxymethyl chitosan (CMCS), multiple hydrogen bonds and electrostatic interactions in the polymer to fabricate SNP@PCN@Gel hydrogels. SNP@PCN@Gel displayed interconnected porous structure, excellent self-healing capacity, low cytotoxicity, good blood compatibility, and robust antibacterial activity. 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