Occurrence and distribution of Streptomyces species causing potato scab in Brazil - Characterization of two new species Streptomyces pococaldensis sp. nov. and Streptomycesshimoyamaii sp. nov.

Occurrence and distribution of Streptomyces species causing potato scab in Brazil - Characterization of two new species Streptomyces pococaldensis sp. nov. and Streptomycesshimoyamaii sp. nov. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Occurrence and distribution of Streptomyces species causing potato scab in Brazil - Characterization of two new species Streptomyces pococaldensis sp. nov. and Streptomycesshimoyamaii sp. nov. Suzete Aparecida Lanza Destéfano, Lucas Vitor, Danilo Trabuco Amaral, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9021977/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 5 You are reading this latest preprint version Abstract Potato scab, caused by species of the genus Streptomyces is a significant disease affecting potato production worldwide, leading to economic losses. This study aimed to investigate the occurrence and distribution of Streptomyces species associated with potato scab in Brazil and to characterize two new species. A total of 91 Streptomyces strains were isolated from symptomatic potato tubers collected across seven states. Morphological, molecular, and pathogenicity assays were performed to classify the isolates. The identification of Streptomyces species was carried out using PCR-RFLP of the atpD gene, sequencing of housekeeping genes, and multilocus sequence analysis (MLSA). Phylogenetic analysis confirmed that two strains, IBSBF 2435T and IBSBF 2507T, represent novel species. Genome sequencing and in silico DNA-DNA hybridization further supported their classification as distinct taxa. The study highlights the presence of pathogenic Streptomyces species in Brazilian potato fields, underscoring the importance of accurate identification for disease management. The characterization of new species expands knowledge on the diversity of phytopathogenic Streptomyces and provides valuable data for further studies on their pathogenic mechanisms and control strategies. According to the data, IBSBF 2435ᵀ and IBSBF 2507ᵀ represent two new Streptomyces species related to potato scab. The proposed names for these strains are Streptomyces pococaldensis sp. nov. (IBSBF 2435ᵀ = CBMAI 2707ᵀ = ICMP 24293ᵀ = MUM 22.66ᵀ) and Streptomyces shimoyamaii sp. nov. (IBSBF 2507ᵀ = CBMAI 2709ᵀ = ICMP 24295ᵀ = MUM 22.64ᵀ). Plant Disease Common scab Taxonomic Phylogenetic delineation Biogeographical distribution Full Text Supplementary Files FigureSupplementary1FigureS1.png FigureSupplementary2FigureS2.png FigureSupplementary3FigureS3.png FigureSupplementary4FigureS4.png TableSupplementary1TableS1.docx TableSupplementary2TableS2.docx Cite Share Download PDF Status: Under Review Version 1 posted Reviewers agreed at journal 05 May, 2026 Reviewers invited by journal 30 Mar, 2026 Editor invited by journal 25 Mar, 2026 Editor assigned by journal 24 Mar, 2026 First submitted to journal 03 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9021977","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":614724607,"identity":"a3318163-77bf-44c8-b8ac-c0df49cd3f4b","order_by":0,"name":"Suzete Aparecida Lanza 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