Exploring the potential of three-layered self-assembled biomaterial-based dry powder inhaler for enhanced structural integrity and lung deposition of bioactive Lysozyme

Exploring the potential of three-layered self-assembled biomaterial-based dry powder inhaler for enhanced structural integrity and lung deposition of bioactive Lysozyme | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Exploring the potential of three-layered self-assembled biomaterial-based dry powder inhaler for enhanced structural integrity and lung deposition of bioactive Lysozyme Pradip Thakare, Bothiraja Chellampillai, Vijaykumar Kuvar, Vaibhav Shinde, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3867336/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 26 Jun, 2024 Read the published version in BioNanoScience → Version 1 posted 7 You are reading this latest preprint version Abstract Aquasome (AQ) is one of the most recently developed self-assembled novel nanocarrier systems that is predominantly effective due to its property of maintaining the structural integrity of proteins, peptides, and delicate bioactive molecules. The objective of the present study was to investigate the feasibility of AQ as a carrier for dry powder inhalers (DPI) to achieve enhanced aerosol performance for Lysozyme (LYS). The water-in-water emulsification method was explored to prepare AQ by using gelatin and cellobiose. The lyophilized LYS-loaded AQ-based dry powder inhaler (DPI) (AQ/LYS-DPI) was evaluated and compared with commercial form of DPI (C-DPI) for its physicochemical properties, in vitro lung deposition, circular dichroism (CD), haemolysis assay, and toxicity study. The developed AQ/LYS-DPI had a particle size of 104.3 ± 3.8 µm, drug loading of 88.70 ± 2.4, and zeta potential of -8.4 mV with sustained release up to 18 h. The mass median aerodynamic diameter (MMAD) and fine particle fraction (FPF) of AQ/LYS-DPI were found to be 2.43 ± 0.27 µm and 61.52 ± 0.38 %, respectively, reflecting enhanced lung deposition. The CD, hemolytic activity, and toxicity study revealed better structural integrity and biocompatibility with no signs of inflammation or toxicity in the formulation. It was observed that the developed AQ/LYS-DPI effectively controls the structural integrity of lysozyme and can be explored as a novel carrier for pulmonary administration of bioactive molecules. Lysozyme Aquasome Proteins and peptide Structural integrity Dry powder inhaler Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 26 Jun, 2024 Read the published version in BioNanoScience → Version 1 posted Editorial decision: Revision requested 28 May, 2024 Reviews received at journal 23 Apr, 2024 Reviewers agreed at journal 23 Apr, 2024 Reviewers invited by journal 22 Jan, 2024 Editor assigned by journal 18 Jan, 2024 Submission checks completed at journal 18 Jan, 2024 First submitted to journal 15 Jan, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3867336","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":267812149,"identity":"af02e970-2029-45f9-bfdb-b5489ca1bc08","order_by":0,"name":"Pradip Thakare","email":"","orcid":"","institution":"Bharati Vidyapeeth (Deemed to be) University, Poona College of Pharmacy","correspondingAuthor":false,"prefix":"","firstName":"Pradip","middleName":"","lastName":"Thakare","suffix":""},{"id":267812150,"identity":"2f836b90-9c90-4f1d-a395-e7a64cc3f9f8","order_by":1,"name":"Bothiraja Chellampillai","email":"","orcid":"","institution":"Bharati 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