Prophylactic effect of Abelmoschus esculentus seed extract on some key parameters in asthmatic mice: bioactive compound analysis and histopathological examination

Prophylactic effect of Abelmoschus esculentus seed extract on some key parameters in asthmatic mice: bioactive compound analysis and histopathological examination | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Prophylactic effect of Abelmoschus esculentus seed extract on some key parameters in asthmatic mice: bioactive compound analysis and histopathological examination Suleman Hussain Shah, Ghulam Murtaza, Mubashar Hussain, Razia Iqbal, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8000549/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Seeds from Abelmoschus esculentus have strong anti-inflammatory properties and may effectively treat inflammatory diseases. This study explores the therapeutic potential of A. esculentus seed extract on bioactive components, hematological parameters, liver function tests, leukocytes, cytokines, and histology in the OVA-induced untreated asthmatic group. Mice were divided into six groups: the normal group, groups treated with A. esculentus (10, 15, and 20 mg/mL), a dexamethasone group (3 mg/mL), and an asthmatic control group. On days 1 and 14 after acclimation, all groups except the normal control received intraperitoneal injections of ovalbumin, followed by oral doses of A. esculentus (10, 15, and 20 mg/mL) or dexamethasone (3 mg/mL) during 15th to 26th day of the experiment. All treated groups were exposed to ovalbumin inhalation for three days afterward. The bioactive components in the acetonic extract were identified through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The primary bioactive compounds identified were fatty acids, flavonoids, and phenolics. Mice given the extract showed improved liver enzyme levels, reduced leukocyte infiltration, alleviated airway constriction, lower cytokine levels (IgE, IL-4, IL-5, IL-10, IL-13, and IL-17A), and normalized blood indices. In conclusion, the seed extract of A. esculentus has significant potential to reduce asthma symptoms in the OVA-induced untreated asthmatic mouse model. Abelmoschus esculentus Mice Ovalbumin Interleukins Histology Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8000549","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":546328367,"identity":"e7c3edfc-9e15-4cc6-be52-f3300f21bb96","order_by":0,"name":"Suleman Hussain 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examination","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Abelmoschus esculentus, Mice, Ovalbumin, Interleukins, Histology","lastPublishedDoi":"10.21203/rs.3.rs-8000549/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8000549/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSeeds from \u003cem\u003eAbelmoschus esculentus\u003c/em\u003e have strong anti-inflammatory properties and may effectively treat inflammatory diseases. This study explores the therapeutic potential of \u003cem\u003eA. esculentus\u003c/em\u003e seed extract on bioactive components, hematological parameters, liver function tests, leukocytes, cytokines, and histology in the OVA-induced untreated asthmatic group. Mice were divided into six groups: the normal group, groups treated with \u003cem\u003eA. esculentus\u003c/em\u003e (10, 15, and 20 mg/mL), a dexamethasone group (3 mg/mL), and an asthmatic control group. On days 1 and 14 after acclimation, all groups except the normal control received intraperitoneal injections of ovalbumin, followed by oral doses of \u003cem\u003eA. esculentus\u003c/em\u003e (10, 15, and 20 mg/mL) or dexamethasone (3 mg/mL) during 15th to 26th day of the experiment. All treated groups were exposed to ovalbumin inhalation for three days afterward. The bioactive components in the acetonic extract were identified through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The primary bioactive compounds identified were fatty acids, flavonoids, and phenolics. Mice given the extract showed improved liver enzyme levels, reduced leukocyte infiltration, alleviated airway constriction, lower cytokine levels (IgE, IL-4, IL-5, IL-10, IL-13, and IL-17A), and normalized blood indices. In conclusion, the seed extract of \u003cem\u003eA. esculentus\u003c/em\u003e has significant potential to reduce asthma symptoms in the OVA-induced untreated asthmatic mouse model.\u003c/p\u003e","manuscriptTitle":"Prophylactic effect of Abelmoschus esculentus seed extract on some key parameters in asthmatic mice: bioactive compound analysis and histopathological examination","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-18 17:04:27","doi":"10.21203/rs.3.rs-8000549/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a74069ff-cdb3-429c-a497-2fa3434e20e7","owner":[],"postedDate":"November 18th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-11-21T19:23:26+00:00","versionOfRecord":[],"versionCreatedAt":"2025-11-18 17:04:27","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8000549","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8000549","identity":"rs-8000549","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}