Caloric and Time Restriction Diets Improve Acute Kidney Injury in Experimental Menopausal Rats: Role of SIRT1 and TGF-β1

Background: Estrogen has a protective effect on acute kidney injury (AKI). In addition, reducing daily calorie intake prevents disease. The aim of this study was to determine the effect of calorie restriction (CR) and time restriction (TR) diets on the expression of SIRT1, TGF-β1 and other indicators in the presence or absence of Ovaries in female rats with AKI.MethodsFemale rats were divided into two groups of resected ovaries (OVX) and sham and were subjected to CR and TR diets for eight weeks. AKI was then induced by glycerol injection and renal injury indices and biochemical parameters were measured before and after AKI.ResultsAfter AKI, the levels of urine albumin excretion rate, urea, and creatinine in serum, and TGF-β1 increased, while GFR and SIRT1 decreased in kidney tissue. CR improved kidney indicators and caused a reduction in TGF-β1 and an increase in SIRT1 in ovary-intact rats. Moreover, CR prevented TAC decrease and MDA increase resulting from AKI. Before AKI, an increase in body weight, fasting blood sugar (FBS), LDL, TG, and TC and a decrease in HDL were observed in OVX rats compared to sham rats, but CR prevented these changes. The effects of TR were similar to those of CR in all indicators except for TGF-β1, SIRT1, urea, creatinine, and albumin. ConclusionThis study showed that CR diet is more effective than TR diet in preventing AKI by increasing SIRT1 and reducing TGF-β1 in ovary-intact animals.