Formulation and Evaluation of Hyaluronate-Coated Novasomes Loaded with Beta vulgaris subsp. Cicla Arial Parts Extract (Swiss Chard) Cultivated in Egypt Along with Phytochemical Investigation

Formulation and Evaluation of Hyaluronate-Coated Novasomes Loaded with Beta vulgaris subsp. Cicla Arial Parts Extract (Swiss Chard) Cultivated in Egypt Along with Phytochemical Investigation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Formulation and Evaluation of Hyaluronate-Coated Novasomes Loaded with Beta vulgaris subsp. Cicla Arial Parts Extract (Swiss Chard) Cultivated in Egypt Along with Phytochemical Investigation Doaa A. H. Deabes, Eman A.W. El-abd, Alaa M. Saleh, Eman S. Shalaby This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8535761/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 12 You are reading this latest preprint version Abstract Arial parts extracts of Beta vulgaris subsp. Cicla (BV) along with their novasomes coated with hyaluronate formulations (BV1 and BV2) were investigated for their antimicrobial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231 and their scavenging potential against (DPPH) radical. The selected formulation BV1 was tested for encapsulation efficiency, particle size zeta potential, FTIR and TEM., Fifteen chromatographic peaks were identified by GC/MS analysis represented (89.64%) from BV dichloromethane fraction, the most abundant compound was oleic acid (30.52%), followed by Palmitic acid (21.92%)and Stearic acid (10.50%) triterpenes and sterol also identified. LC/MS/MS of total ethanol extract results the identification of 33 compounds including flavonoids, phenolic acids and their derivatives which represented the major compounds also coumarin, fatty acid and amino acid were identified. In addition to isolation of four compounds Oleanolic acid and campsterol from dichloromethane fraction while isovitexin and Apigenin-O- hexoside from ethanol extract. The crude extract showed antimicrobial activity against C.albicans only whilenovasomes formulationsBV1 was active against both bacteria and yeast. As for BV2, it was active against bacteria only. Regarding antioxidant activity, crude extract together with both formulas showed ability to scavenge DPPH free radical. Biological sciences/Biochemistry Biological sciences/Biotechnology Physical sciences/Chemistry Biological sciences/Drug discovery Biological sciences/Microbiology Biological sciences/Plant sciences Antimicrobial Antioxidant GC/MS LC/MS/MS Novosomes Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 09 Feb, 2026 Reviews received at journal 07 Feb, 2026 Reviews received at journal 31 Jan, 2026 Reviewers agreed at journal 26 Jan, 2026 Reviews received at journal 24 Jan, 2026 Reviewers agreed at journal 23 Jan, 2026 Reviewers agreed at journal 23 Jan, 2026 Reviewers invited by journal 23 Jan, 2026 Editor assigned by journal 23 Jan, 2026 Editor invited by journal 19 Jan, 2026 Submission checks completed at journal 12 Jan, 2026 First submitted to journal 12 Jan, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8535761","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":581396514,"identity":"7d895bec-964f-49f8-9365-6ee1d48dcd2f","order_by":0,"name":"Doaa A. H. 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