Inoculation of microalgae distinctly shapes the structure and enhances the stability of eukaryotic plankton community in shrimp rearing water

Inoculation of microalgae distinctly shapes the structure and enhances the stability of eukaryotic plankton community in shrimp rearing water | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Inoculation of microalgae distinctly shapes the structure and enhances the stability of eukaryotic plankton community in shrimp rearing water Jie Xiang, Chen Lian, Huifeng Cai, Jiangdong Ke, Heng Ni, Jinyong Zhu, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4542405/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Inoculating microalgae has been verified to have the potential to improve the microenvironment of shrimp rearing water and modulate bacterial communities. However, the impact of this intervention on the water eukaryotic plankton community remains relatively unexplored. In this study, we inoculated two indigenous microalgae, Nannochloropsis oculata and Thalassiosira weissflogii, into shrimp rearing water to elucidate the dynamics and interactions among microalgae, nutrient factors, and the small-sized (0.22-3 μm) and large-sized (3-200 μm) eukaryotic plankton community. Our findings demonstrated significant differences in the composition and diversity the small-sized (0.22-3 μm) and large-sized (3-200 μm) eukaryotic plankton community under various microalgae treatments. PERMANOVA (permutational multivariate analysis of variance) revealed significant differences among treatments in terms of rearing time and Size fraction. Moreover, partial least squares path modeling (PLS-PM) analysis showed that microalgae and nitrite could directly affect the small-sized community or indirectly affect them through trophic interactions. According to the neutral community model, inoculation of microalgae amplifies the relative contribution of deterministic processes in community assembly. Furthermore, the average variation degree (AVD) index analysis results indicated that the inoculation of N. oculata was particularly effective in enhancing community stability. These results indicate the importance of microalgae in shaping the structure and stability of eukaryotic plankton communities in shrimp rearing water, which holds great promise for the understanding and development of technologies aimed at improving the quality of rearing water, thereby contributing to more successful shrimp cultivation practices. Nannochloropsis oculata Thalassiosira weissflogii Environmental factors Diversity Community stability Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementaryfile1.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4542405","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":317923726,"identity":"9ca0b7fe-04bd-486f-a86f-80739ff6ae04","order_by":0,"name":"Jie Xiang","email":"","orcid":"","institution":"Ningbo University","correspondingAuthor":false,"prefix":"","firstName":"Jie","middleName":"","lastName":"Xiang","suffix":""},{"id":317923727,"identity":"7738b74a-2809-443b-8c5c-274b77d1ee8b","order_by":1,"name":"Chen Lian","email":"","orcid":"","institution":"Ningbo 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