Single-cell RNA sequencing reveals the heterogeneity of peripheral blood mononuclear cells in renal cell carcinoma

Background: Peripheral blood mononuclear cells (PBMCs) are closely related to tumors, and the functions of T cells and B cells are related to tumor occurrence, development, and prognosis. Conventional second-generation sequencing cannot distinguish the characteristics of various peripheral blood mononuclear cell subsets, so it is impossible to study PBMCs accurately. Recently, single-cell sequencing technology has improved, and it provides a tool to study immune cells in circulating blood. Methods PBMCs from patients with renal cell carcinoma (RCC) were sequenced to explore the characteristics of PBMC subsets in patients with renal cell carcinoma, mainly B cells and Treg cells, to study the relationship between renal cell carcinoma and peripheral blood immune cells. Results our PBMC study of RCC patients successfully separated several types of immune cells from the blood. PBMCs can map the tumor to a certain extent. Conclusions We can expand the samples based on the current research, and further research will discover more meaningful information related to cancer.