Improved data analysis for non-invasive, label-free biomolecular characterisation of single bacterial cells via Raman microspectroscopy

Improved data analysis for non-invasive, label-free biomolecular characterisation of single bacterial cells via Raman microspectroscopy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Brief Communication Improved data analysis for non-invasive, label-free biomolecular characterisation of single bacterial cells via Raman microspectroscopy Christoph Engl, Zechuan Gong, Andrey Sapelkin This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6673956/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract The biological application of Raman microspectroscopy - a non-invasive, label-free, imaging technology for detecting biomolecules – has been limited by the challenging interpretation of cellular spectra. Using an optimised methodology, we deliver quantitative biologically relevant information to characterise biomolecular variation within cell populations that provides a clear distinction between cellular states. This approach yields content-rich, high-resolution molecular analysis of complex cell populations without the need for genetic modification, fluorescent reporters, or disruptive techniques. Biological sciences/Cell biology/Cellular imaging Biological sciences/Biological techniques/Optical spectroscopy/Raman spectroscopy Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupplementaryInformation.pdf Supplementary Information Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6673956","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Brief Communication","associatedPublications":[],"authors":[{"id":472066938,"identity":"eb10ac40-f991-48c9-8594-4ca7227ce77b","order_by":0,"name":"Christoph Engl","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAw0lEQVRIiWNgGAWjYNCCAhsgwdwAZksQp8UgDUgwkqblMAla+BuYjz38YnBedu2MxAaGHzUMiTMbCGiROMCWbixjcNt4243EBsaeYwyJswk6iYHHTFrC4HYiSAsDbwND4jzCWvi/AbWcA2th/EucFh42yQ8GB8BamEG2EHSYxGE2M2kGg2TjbWceNhyWOSZhTND7/O3NzyR/VNjJbjuefPDhmxob2RkHCFnDDEQ80Eg5QHREMv6Ax+MoGAWjYBSMAiwAAOlKPo3tBlFxAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0003-0335-267X","institution":"Queen Mary University of London","correspondingAuthor":true,"prefix":"","firstName":"Christoph","middleName":"","lastName":"Engl","suffix":""},{"id":472066939,"identity":"f53e5e05-e8ce-4348-ac9b-05e836281390","order_by":1,"name":"Zechuan Gong","email":"","orcid":"","institution":"Queen Mary University of London","correspondingAuthor":false,"prefix":"","firstName":"Zechuan","middleName":"","lastName":"Gong","suffix":""},{"id":472066940,"identity":"74d9b305-3e22-4988-9948-9dff0b81efad","order_by":2,"name":"Andrey Sapelkin","email":"","orcid":"https://orcid.org/0000-0001-7648-8215","institution":"Queen Mary University of London","correspondingAuthor":false,"prefix":"","firstName":"Andrey","middleName":"","lastName":"Sapelkin","suffix":""}],"badges":[],"createdAt":"2025-05-15 15:21:57","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6673956/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6673956/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":85815417,"identity":"9ed5a3b4-63d5-41c2-b6cd-1f6661e64c19","added_by":"auto","created_at":"2025-07-02 05:13:13","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1066569,"visible":true,"origin":"","legend":"Article File","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6673956/v1_covered_68118c9b-a9d5-4de3-b3bd-60e0e78b4168.pdf"},{"id":85814992,"identity":"d1a54d89-90ba-474e-9ef4-68cfd378c654","added_by":"auto","created_at":"2025-07-02 05:05:12","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1113567,"visible":true,"origin":"","legend":"Supplementary Information","description":"","filename":"SupplementaryInformation.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6673956/v1/57f4eacf9eff579a13f66db4.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"\u003cp\u003eImproved data analysis for non-invasive, label-free biomolecular characterisation of single bacterial cells via Raman microspectroscopy\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-6673956/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6673956/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"The biological application of Raman microspectroscopy - a non-invasive, label-free, imaging technology for detecting biomolecules – has been limited by the challenging interpretation of cellular spectra. 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