Aceptable day-to-day variability of cerebral perfusion using a non-invasive image derived input function in [15O]H2O PET

Aceptable day-to-day variability of cerebral perfusion using a non-invasive image derived input function in [15O]H2O PET | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 16 January 2026 V1 Latest version Share on Aceptable day-to-day variability of cerebral perfusion using a non-invasive image derived input function in [15O]H2O PET Authors : Thomas Ehlig Hjermind Justesen 0000-0002-6431-1268 [email protected] , Jørgen Rungby , Markus Nowak Lonsdale , Inge Lise Rasmussen , Stefan Fuglsang , Carl-Johan Boraxbekk , Eva Irene Bossano Prescott , and Lisbeth Marner Authors Info & Affiliations https://doi.org/10.22541/au.176857035.57040678/v1 134 views 69 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The PET radiotracer [ 15 O]H 2 O is the reference standard for quantifying myocardial blood flow and cerebral blood flow. However, the requirement for an invasive arterial input function limits clinical applicability. An alternative, non-invasive image-derived input function from the left ventricle has been validated for the heart, and recent work has shown that the image-derived input function obtained in an additional scan of the heart can be applied for the brain at rest and during acetazolamide-induced vasodilation. To further establish its clinical and research utility, we investigated the intra-individual day-to-day variability. Eight healthy participants underwent [ 15 O]H 2 O PET to assess cerebral blood flow on 2 occasions within 14 days using an image-derived input function placed in the left ventricle of the heart at rest. Day-to-day variability was assessed with Bland-Altman Plots, correlation coefficients, and Coefficient of Variation across three larger and three smaller brain regions. Day 1 and Day 2 measurements showed strong linear associations at rest (r = 0.75–0.85), during stress (r = 0.78–0.86) and for perfusion capacity (r = 0.75–0.82). Limits of agreement ranged from -0.096 to 0.121 mL/(g·min) at rest, -0.164 to 0.164 mL/(g·min) during stress and -0.255 to 0.189 mL/(g·min) for perfusion capacity. Coefficient of variation ranged from 11.9% to 12.6% at rest, 12.3% to 12.9% during stress and 7.8% to 8.2% for perfusion capacity. This non-invasive method is reproducible on a group level with coefficients of variation below 13% and is thus suitable for detecting group-level changes in intervention studies, although individual variability is substantial. Supplementary Material File (figures.docx) Download 178.88 KB File (tables.docx) Download 140.23 KB File (test-retest 15oh2o brain perfusion_ejn.docx) Download 314.96 KB Information & Authors Information Version history V1 Version 1 16 January 2026 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords (neuro)physiology functional neuroimaging kinetics positron emission tomography regional cerebral blood flow Authors Affiliations Thomas Ehlig Hjermind Justesen 0000-0002-6431-1268 [email protected] Bispebjerg Hospital View all articles by this author Jørgen Rungby Steno Diabetes Center Copenhagen View all articles by this author Markus Nowak Lonsdale Bispebjerg Hospital View all articles by this author Inge Lise Rasmussen Bispebjerg Hospital View all articles by this author Stefan Fuglsang Bispebjerg Hospital View all articles by this author Carl-Johan Boraxbekk Bispebjerg Hospital Institut for Idraetsmedicin Kobenhavn View all articles by this author Eva Irene Bossano Prescott Bispebjerg Hospital View all articles by this author Lisbeth Marner Herlev Hospital View all articles by this author Metrics & Citations Metrics Article Usage 134 views 69 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Thomas Ehlig Hjermind Justesen, Jørgen Rungby, Markus Nowak Lonsdale, et al. Aceptable day-to-day variability of cerebral perfusion using a non-invasive image derived input function in [15O]H2O PET. Authorea . 16 January 2026. DOI: https://doi.org/10.22541/au.176857035.57040678/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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