Improving the detection ofA. cantonensisin brain tissues of mammalian hosts

Angiostrongylus cantonensis is an invasive nematode parasite that can cause eosinophilic meningitis in many vertebrate hosts, including humans. This parasite is spreading rapidly through the six continents, with Europe being the final frontier. Sentinel surveillance may be a cost-effective surveillance strategy to monitor the arrival of this pathogen to new geographical regions as this can be easily expanded to incorporate symptomatic wildlife. Necropsy and tissue digestion techniques are often used to recover helminth parasites from vertebrate host tissues, however their application to detect brain parasites is poorly utilized. Here we show that employing these techniques in combination can 1) help resolve false positive and negative animals, 2) provide accurate parasitic load values and 3) establish an accurate prevalence of A. cantonensis . Our adapted tissue digestion technique can be easily performed, especially in wildlife hospitals where animal infections often precede human cases. Early detection of the parasite increases the efficacy of prevention, treatment, and disease control strategies not only in humans, but also in susceptible animal populations. Key Findings Optimized brain digestion techniques can detect parasitic helminths ( A. cantonensis ) in mammalian hosts. Accuracy identifying neurotropic parasitic infections can be increased if a standard digestion protocol is applied. The technique presented here can be easily implemented to detect brain nematodes in wildlife hospitals.