High-dimensional solid-state NMR facilitated by transverse-mixing optimal control

High-dimensional solid-state NMR facilitated by transverse-mixing optimal control | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article High-dimensional solid-state NMR facilitated by transverse-mixing optimal control Rasmus Linser, Alexander Klein, Jan Blahut, Zdeněk Tošner, Dmitrii Blokhin, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8003957/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Owing to fast magic-angle spinning, solid-state nuclear magnetic resonance has evolved as a versatile method to decipher protein structure, dynamics, and chemical properties. While higher-dimensional approaches such as 4D and 5D correlation spectra are generally capable of exceeding the established molecular-weight limitations by ameliorating signal overlap, their intrinsic implications for sensitivity and measurement times required have severely limited these important prospects in practice. Here we show that the extensive use of dedicated transverse-mixing optimal control pulses (TROPs) in high-dimensional experiments to enable concomitant transfer of complex signals along complex magnetization transfer pathways can reduce the necessary measurement time by an order of magnitude. Owing to the multiplicative benefits of the enhancement for successive indirect chemical-shift dimensions, combined with non-linear benefits upon spectral reconstruction, the combination of non-uniformly sampled, higher-dimensional approaches with an extensive use of TROPs hence presents itself as transformative for the conventional limitations of solid-state NMR. Biological sciences/Structural biology/NMR spectroscopy/Solid-state NMR Biological sciences/Structural biology/NMR spectroscopy NMR Spectroscopy Sensitivity enhancement Higher-dimensionality experiments Resonance assignment Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SI.pdf Supplementary information pdf Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8003957","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":547544003,"identity":"47261b81-ad63-463a-9dff-931a655ff692","order_by":0,"name":"Rasmus 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