Bulk RNAseq of Th1, Th1/17, and Th17 cell subsets in endometriosis patients and controls

This dataset contains bulk RNA sequencing (RNAseq) differential gene expression results from circulating Th1, Th1/17, and Th17 cell subsets derived from endometriosis patients and healthy controls. The dataset supports analyses presented in a doctoral thesis submitted to Queen’s University. At the time of deposit, the data are under embargo as they are associated with unpublished research findings. Overall, we aimed to gain a comprehensive understanding of transcriptional profiles of circulating Th1, Th1/17, and Th17 cells in endometriosis patients (n=10) as compared to healthy controls (n=12). Briefly, fresh peripheral blood was collected from subjects, peripheral blood mononuclear cells (PBMCs) were stained for FACS and sorted into viable populations of Th17 (CD3+CD4+IFNg-IL-17+), Th1/17 (CD3+CD4+IFNg+IL-17+), and Th1 (CD3+CD4+IFNg+IL-17-) cells using BD FACSAria™ III. RNA was then isolated from Th1, Th1/17, and Th17 cell populations from patients and controls. Samples were then quality checked and sent for bulk RNAseq at McGill Genome Centre (MGC, Canada) using Illumina NovaSeq X Plus 10B (Illumina, USA) and subsequent bioinformatic analyses, as per standard pipelines. This dataset specifically depicts fold change values for differentially expressed genes in each comparison. Collectively, our findings reveal significant immune remodeling in endometriosis and highlight a distinct, aberrant Th17 cell phenotype, contributing to endometriosis pathophysiology.