Genotyping and Pre-treatment drug resistance surveillance among ART- naive HIV-1 patients in Tunisia.

Genotyping and Pre-treatment drug resistance surveillance among ART- naive HIV-1 patients in Tunisia. | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 24 January 2025 V1 Latest version Share on Genotyping and Pre-treatment drug resistance surveillance among ART- naive HIV-1 patients in Tunisia. Authors : Ibtihel Smeti [email protected] , Salma Mhalla , Ikbel Kooli , Yosr Kadri , Hajer Rhim , Ons Haddad , Amel Nabli , Nabil Abid , Mohamed Chakroun , and Maha MASTOURI Authors Info & Affiliations https://doi.org/10.22541/au.173771933.33397780/v1 235 views 116 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Drug resistance to Human Immunodeficiency Virus (HIV) is a major factor in treatment failure. Although this resistance is mostly related to drug exposure, treatment-naïve patients living with HIV have also been shown to develop primary drug resistance. Resistance monitoring before treatment prevents the first-line antiretroviral therapy (ART) failure and improves treatment outcomes. Very few data are available on the prevalence of HIV drug-resistance in Tunisia, especially among ART-naïve patients. This study aimed to investigate HIV-1 drug resistance patterns among ART-naïve Tunisian and African individuals. The study cohort included 77 treatment-naïve PLHIV who visited the HIV Center of Monastir to initiate ART. Viral RNAs were partially sequenced in the RT and PR regions using Sanger sequencing. Subtypes and Drug Resistance mutations (DRMs) were determined using the Stanford HIV Database. HIV-1 subtying revealed the predominance of the CRF02_AG, followed by the subtype B. 12,9% of variants were shown to have at least one Surveillance DRM (SDRM) in the PR/RT region. Most observed resistance cases (60%) were noted for RT inhibitors, with K103N the most involved SDRM-inducing resistance to first and second generation NNRTs (efavirenz, nevirapine, and rilpivirine). In the PR gene, resistance was found in four samples on 46 and 85 codons, affecting the sensitivity for Atazanavir and Lopinavir drugs. This study provides the first baseline information for pre-treatment drug resistance surveillance of HIV-1 variants in Tunisia, and shows considerable mutations, which may impede patient management. Supplementary Material File (medical virology sub smeti i. mhalla s.docx) Download 71.18 KB File (tables.docx) Download 15.36 KB Information & Authors Information Version history V1 Version 1 24 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords anti-retrovirus drug antiviral agents human immunodeficiency virus infection non-nucleoside reverse transcriptase inhibitor nucleoside analog reverse transcriptase protease inhibitor resistance virus classification Authors Affiliations Ibtihel Smeti [email protected] Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Salma Mhalla Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Ikbel Kooli Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Yosr Kadri Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Hajer Rhim Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Ons Haddad Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Amel Nabli Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Nabil Abid Universite de Monastir Faculte de Pharmacie de Monastir View all articles by this author Mohamed Chakroun Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Maha MASTOURI Hopital Universitaire Fattouma Bourguiba a Monastir View all articles by this author Metrics & Citations Metrics Article Usage 235 views 116 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Ibtihel Smeti, Salma Mhalla, Ikbel Kooli, et al. Genotyping and Pre-treatment drug resistance surveillance among ART- naive HIV-1 patients in Tunisia.. Authorea . 24 January 2025. DOI: https://doi.org/10.22541/au.173771933.33397780/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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